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Citations for Addgene :
551 - 600 of 686 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the RBD expression vectors were co-transfected into Expi293F cells together with the secreted BirA-Flag plasmid (Addgene) in the presence of 100 μM biotin.
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bioRxiv - Developmental Biology 2020Quote: ... The 3x FLAG 2x Strep tag sequence was amplified from AAVS1 Puro Tet3G 3xFLAG Twin Strep (Addgene, # 92099) (Dalvai et al. ...
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bioRxiv - Cancer Biology 2020Quote: Expression plasmid encoding N-terminally Flag-tagged hFOXO1-3A (#13508) was purchased from Addgene (Cambridge, MA, pCDNA3 backbone). FOXO1-3A has Ala residue substitutions at Thr-24 ...
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bioRxiv - Cell Biology 2021Quote: ... a vector for overexpression of constitutively active FOXO1 with mutated phosphorylation site (pcDNA3 Flag FKHR AAA mutant was a gift from Kunliang Guan (Addgene plasmid #13508; http://n2t.net/addgene:13508; RRID:Addgene_13508) (32) ...
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bioRxiv - Neuroscience 2023Quote: lenti SYN-FLAG-dCas9-KRAB-MeCP2 was a gift from Jeremy Day (Addgene plasmid # 155365; http://n2t.net/addgene:155365; RRID:Addgene_155365)
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bioRxiv - Developmental Biology 2023Quote: ... The full-length RUVBL1 with N-terminal 3×FLAG tag (pCDNA-3xFLAG-Pontin) was obtained from Addgene (51635). All cell lines were grown in DMEM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The constructs were LR-recombined into pDest-pcDNA3.1 with N-terminal FLAG-tag or into pLIX_403 (Plasmid #41395, Addgene) with C-terminal GFP-tag ...
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bioRxiv - Cancer Biology 2023Quote: The YAP constitutively active overexpression lentiviral vector was generated using pCMV-Flag YAP127/128/131/381A plasmid (Addgene) and Duet011 lentiviral plasmid.
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bioRxiv - Physiology 2023Quote: ... plus a five-amino-acid linker plus a C-terminal flag tag was cloned into the pENN.AAV.CB7.CI.pm20d1flag.WPRE.rBG vector (Addgene plasmid no. 132682). AAV8-GFP (pENN.AAV.CB7.CI.eGFP.WPRE.rBG),used as control ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE(79) was a gift from Matthew Banghart (Addgene plasmid # 166970 ; http://n2t.net/addgene:166970 ; RRID:Addgene_166970) which was modified to express the oScarlet fluorophore in place of the original mCherry.
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bioRxiv - Molecular Biology 2023Quote: ... we generated pcDNA3.1-EGFR-flag-TurboID by substituting APEX2 with a GeneStrands fragment (Eurofins) encoding TurboID (Addgene: #124646). From these vectors EGFR-flag-APEX2 and EGFR-flag-TurboID were subcloned into pcDNA5/FRT/TO to generate pcDNA5/FRT/TO-EGFR-flag-APEX2 and pcDNA5/FRT/TO-EGFR-flag-TurboID ...
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bioRxiv - Molecular Biology 2024Quote: ... pcDNA3.1-FLAG-gp130:sarA chimera codon-optimized for mammalian expression were previously described in and available from Addgene. Site-directed mutagenesis was carried out using Quikchange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... pGEX6P1-DEST-FLAG was a gift from Andrew Jackson & Martin Reijns (Addgene plasmid # 119754; http://n2t.net/addgene:119754; RRID:Addgene_119754).CAPS-GST protein was expressed in BL21Star bacteria (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were co-transfected with pcDNA3.0 plasmids containing human CDC50A (NM_018247, N-terminal FLAG tag; RRID: Addgene_203694) and ATP10B variants (O94823.2 ...
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bioRxiv - Cell Biology 2024Quote: ... AOX overexpression plasmid was achieved with pCW57.1_AOX-FLAG (pCW57.1_AOX-FLAG was a gift from David Sabatini & Jessica Spinelli (Addgene plasmid # 177984; http://n2t.net/addgene:177984 ; RRID:Addgene_177984)) ...
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bioRxiv - Genomics 2019Quote: ... coli expressing Streptococcus Pyogenes Cas9 carrying a C-terminal fusion to a hexa-histidine tag from the pET-28b-Cas9-His plasmid (Addgene http://www.addgene.org/47327) (56) ...
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bioRxiv - Biochemistry 2020Quote: hnRNPA2 LC S285C and S329C variants for PRE, insoluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98665, 98667 respectively)
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bioRxiv - Biophysics 2023Quote: ... and its variants were cloned into the pEG-BacMam expression vector with GFP and 8X-His tags at the N-terminus (Addgene, see Table S2). For FLAG-tagged KRAS expression constructs ...
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bioRxiv - Biochemistry 2020Quote: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre was a gift from Robert Singer (Addgene plasmid # 84561; RRID: Addgene_84561). pUbC-OsTIR1-myc-IRES-scFv-sfGFP was a gift from Robert Singer (Addgene plasmid # 84563 ...
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bioRxiv - Biochemistry 2020Quote: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre was a gift from Robert Singer (Addgene plasmid # 84561; RRID: Addgene_84561). pUbC-OsTIR1-myc-IRES-scFv-sfGFP was a gift from Robert Singer (Addgene plasmid # 84563 ...
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bioRxiv - Biochemistry 2021Quote: ... pLPC-puro-N-Flag was a gift from Titia de Lange (Addgene plasmid # 12521; http://n2t.net/addgene:12521; RRID: Addgene_12521). The pLPC-puro-N-Flag-NELFE plasmid was obtained by amplification of the human NELFE cDNA (obtained from gene synthesis ...
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bioRxiv - Biochemistry 2021Quote: ... was used to generate pcDNA3.1-p300-FLAG from the plasmid pcDNA3.1-p300-His6 obtained from Addgene (Catalog number 23252) by following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556) ...
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bioRxiv - Cancer Biology 2020Quote: ... pGL3-U6-sgRNA-PGK-puromycin and pST1374-N-NLS-flag-linker-Cas9-D10A were gifts from Xingxu Huang (Addgene plasmid # 51133 ...
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bioRxiv - Cancer Biology 2021Quote: ... pcDNA3-based plasmids encoding FLAG-tagged wild type and SATA (S939A/T1462A)-mutant TSC2(51) were obtained from Addgene. The TSC2-5A (S939A ...
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bioRxiv - Immunology 2019Quote: ... 2×106 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg myc-Rictor plasmid (Addgene). Control HEK293T cells were transfected with myc-Rictor or 10 ng GFP-3xFLAG as described ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... full-length Prdm16 overexpressing plasmid with an in-frame N-terminal FLAG tag was purchased from Addgene (Addgene #15504). Full-length Dnmt1 cDNA clone was obtained from Open Biosystems and further subcloned into the pLVX lentiviral expression vector (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... The pcDNA-FLAG-V5-Nsp10/14/16 vectors were constructed from pDONR223 SARS-CoV-2 Nsp10 (Cat. # 141264, Addgene), Nsp14 (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Either a control vector (c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid #20011; http://n2t.net/addgene:20011; RRID:Addgene_20011(47)) ...
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bioRxiv - Biochemistry 2021Quote: ... Jürg Müller (for generation of pcDNA5.1-FRT/TO-puro-(N)GFP-TEV-FLAG-3C-BAP1) or originated from Wade Harper’s laboratory obtained from Addgene (for generation of pcDNA5-FRT/TO-puro-eGFP-BAP1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Flag-hHOXA1 (pchHOXA1-3XFlag-myc) and Flag-mHoxA1 (pcmHoxa1-3XFlag-myc) were generated by cloning PCR-amplified inserts into a pcDNA3.1 vector (Addgene) featuring a 3XFlag-myc tag sequence in the multiple cloning site ...
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bioRxiv - Cell Biology 2022Quote: ... the defined concentration of viral packaging plasmids VSV-G and GAG-POL for overexpressing Flag-HA-USP1 (Addgene, #22596) and pLV-TetO-ID2 (Addgene plasmid #70764 ...
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bioRxiv - Developmental Biology 2023Quote: The ORF of FLAG-NLS-dCas9-NLS was amplified from the plasmid pWalium20-10XUAS-3XFLAG-dCas9-VPR (Addgene: #78897) (Lin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The pSLIK-Neo-FLAG-hBirA*-GEMC1/MCIDAS plasmids were co-transfected with lentiviral packaging plasmid vectors REV (Addgene 12253)24 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCDH-Flag-c-MYC was a gift from Hening Lin (Addgene plasmid # 102626; http://n2t.net/addgene:102626; RRID: Addgene_102626).
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Biophysics 2024Quote: ... FLAG-Mma PyIRS (Mma PylT/RS) and pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A (G1PylT/RS) tRNA/aminoacyl-tRNA synthetase pairs from Addgene (Watertown ...
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse vsv-plexin-B3 and human pECE-M2-BAIAP2 (IRSP53-Flag) were purchased from Addgene (#68038, #68039, #31656, USA).
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bioRxiv - Physiology 2023Quote: ... FLAG-Mma PyIRS (Mma PylT/RS) and pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A (G1PylT/RS) tRNA/aminoacyl-tRNA synthetase pairs from Addgene (Watertown ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Cas9 protein was produced by the Weizmann Institute of Science Protein Purification Unit using the pET-28b-Cas9-His (Alex Schier Lab Plasmids, Addgene, Cambridge, MA, United States) as a template ...
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bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-APEX plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and APEX from pcDNA3 APEX-nes (Addgene) into XPack CMV constructs (System Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell line inducibly expressing Flag-NSP2 was generated by co-transfecting pcDNA5-FRT-TO-FH-Nsp2 (Addgene plasmid 157683) and pOG44 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The GFP-P2A-FLAG-K(AAA)20-P2A-mKate2 construct was modified based on GFP-P2A-FLAG-K(AAG)20-P2A-RFP (105689, Addgene). For MTS (Mitochondrial targeting sequence)- GFP-K(AAA)20-P2A-mKate2 construction ...
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bioRxiv - Biochemistry 2020Quote: ... H-HCF-1) and full length human THAP11 cDNA with carboxy-terminal FLAG tag (Plasmid #28020; F-THAP11) were obtained from Addgene. Vectors containing full length human THAP1 cDNA with carboxy-terminal 1X FLAG tag (THAP1-F ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse Halo-FMRP construct was generated by replacing the EGFP with the HaloTag coding sequence in the p-EGFP-C1-Flag-mFmr1(wt) vector which was a gift from Stephanie Ceman (Addgene plasmid #87929 ...
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bioRxiv - Cancer Biology 2022Quote: ... were purchased from Horizon Discovery while the lentiviral vectors expressing scrambled shRNA (Sarbassov et al., 2005) and flag-tagged DPYD (Shaul et al., 2014) were bought from Addgene Inc ...
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bioRxiv - Molecular Biology 2020Quote: Human Drosha cDNA with a Flag-tag at the amino-terminus was cloned into pBABE-puro vector (Addgene plasmid#1764) for producing retrovirus of human Drosha wild type (WT) ...
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bioRxiv - Cancer Biology 2020Quote: ... pGL3-U6-sgRNA-PGK-puromycin and pST1374-N-NLS-flag-linker-Cas9-D10A were gifts from Xingxu Huang (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene: 51133). The knockout analysis was performed as previously described(86) ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lines overexpressing SLC7A11 were generated using pLenti-CMV-SLC7A11-sh926R-FLAG-IRES-Hygro (a gift from William Kaelin (Addgene plasmid #118702 ...