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Citations for Addgene :
351 - 400 of 1005 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... pCasTn plasmid cloning: ShCAST genes were PCR amplified from pHelper_ShCAST_sgRNA (Addgene #127921). The vector backbones were amplified from plasmids carrying an RK2 origin of replication with a Kanamycin resistance gene for pKSh2 (Addgene #167515 ...
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bioRxiv - Neuroscience 2020Quote: ... dLight1.1 sequence was PCR amplified from pCMV.dLight1.1 (Addgene #111053, Dr. Lin Tian)56 using Q5 polymerase (primers ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry was PCR amplified from mCherry-Alpha-5-Integrin-12 (Addgene #54970) using forward primer 54970RMmCherryaddNHis_F2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR with pKD3 (pKD3 was a gift from Barry L. Wanner; Addgene plasmid #45604 ...
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bioRxiv - Genetics 2020Quote: ... The His2Av polyA sequence was PCR amplified from pDEST-APLO (Addgene 112805). DNA fragments were assembled together using NEBuilder DNA Assembly (New England Biolabs Inc.).
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bioRxiv - Neuroscience 2020Quote: ... secA5 sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene) and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene) sequences ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24x_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Genomics 2021Quote: ... The PCR amplicon was cloned into a lacZ reporter vector (Addgene #139098) using Gibson assembly (New England Biolabs)57 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MetRSL274G coding sequence was PCR amplified using pMarsL274G construct (Addgene 63177) as a template and inserted into BamHI and MluI sites within the pLV-EF1a-IRES-puro vector (Addgene 85132 ...
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bioRxiv - Cancer Biology 2021Quote: ... TUBA1B was amplified using PCR (donor plasmid: Addgene #57159, from Michael Davidson) and an EGFP-TUBA1B fusion protein was generated with two-stage overhang extension PCR using the TUBA1B and EGFP cDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Cell Biology 2022Quote: ... 24xSuntag (24xGCN4) was PCR-amplified from pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (Addgene, #74928) and inserted between the BsrGI and ApaI sites of pcDNA-MCP-mCherry.
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bioRxiv - Molecular Biology 2022Quote: ... IκBα DNA was PCR amplified from pBABE-GFP-IκBα-wt (Addgene, 15263) and was digested and cloned into pET28-CBP-His6 ...
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bioRxiv - Neuroscience 2022Quote: ... we first PCR amplified myr- 4xSNAPf from pUAS-myr-4xSNAPf (Addgene #87637) using FRT-containing primers ...
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bioRxiv - Genomics 2022Quote: ... KRAB(ZIM3) was PCR-amplified from pLX303-ZIM3-KRAB-dCas9 (Addgene # 154472). The final construct is KRAB(ZIM3)-dCas9 or Zim3-dCas9 for short ...
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with AgeI and XbaI and cloned into cut sites upstream of HaloTag insert in pGW1-Halo.
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with KpnI and SalI and cloned into cut sites upstream of the 2A in the pGW1-2A-GFP vector.
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bioRxiv - Cancer Biology 2023Quote: ... The IRES site was amplified by PCR from pInducer-21 (Addgene #46948). YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Immunology 2023Quote: ... eGFP was amplified via PCR from pLJM-1-eGFP (Addgene: Plasmid #19319) using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1 ...
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bioRxiv - Pathology 2023Quote: Cre recombinase DNA was amplified by PCR using pCAG-Cre (Addgene #13775) as a template ...
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bioRxiv - Cancer Biology 2023Quote: ... The bGH poly(A) signal was PCR amplified from pX459 (Addgene, 62988) using primers forward ...
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR amplicon was cloned into a lacZ-reporter vector (Addgene #139098) or mCherry reporter vector (available upon request ...
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bioRxiv - Microbiology 2023Quote: ... mKO2 was PCR amplified from the template mKO2-N1 (Addgene, Cat. #4625) using primers containing matching restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... and a synthetic intron was PCR-amplified from pDEST-APLO (Addgene 112805) (77) ...
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bioRxiv - Cancer Biology 2023Quote: ... were ordered from GenScript (https://www.genscript.com) and PCR-cloned into pHIV-Luc-ZsGreen (Addgene plasmid #39196) using and EcoRI and XbaI digestion.
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bioRxiv - Neuroscience 2023Quote: ... a Cas9-T2A fragment PCR amplified from pDEST-APIC-Cas9 (Addgene 121657) (9) ...
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR amplifying the ORFs from cDNA templates of HSC70 (19514, Addgene) and LAMP2A (86146 ...
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bioRxiv - Cell Biology 2023Quote: ... with HA-TurboID amplified by PCR from 3xHA-TurboID-NLS_pCDNA3 (Addgene #107171) and inserted with a Gly-Gly-Gly-Ser linker either side of the HA-TurboID sequence ...
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bioRxiv - Plant Biology 2022Quote: ... For this the mNeonGreen coding sequence was amplified using primers mNG-HindIII-F and mNG-stop-EcoRI-R (Table S6) from pPY22 (Addgene plasmid #137082; Yi and Goshima, 2020), introducing a GSGGSG-encoding linker before mNeonGreen in the process) ...
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bioRxiv - Cancer Biology 2021Quote: ... CyclinB1-GFP was amplified using PCR (donor plasmid: Addgene #26061, from Jonathon Pines) and was subcloned into pDONR221 and subsequently subcloned into pLX303 ...
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bioRxiv - Molecular Biology 2020Quote: ... ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425 ...
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bioRxiv - Genetics 2021Quote: ... The sequence of GeneSwitch was amplified by PCR using pElav-GeneSwitch (Addgene: 83957). The backbone vector pElav-GeneSwitch was digested with KpnI ...
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bioRxiv - Developmental Biology 2020Quote: MoxMaple3 was amplified by PCR from the pmCherry-T2A-moxMaple3 (Addgene Plasmid #120875) [43] and inserted into the pUASp by SpeI (3’)/EcoRI (3’) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons of sT and LT gene regions from plasmid RAZ1 (Addgene #114381) were used as target DNA templates (Refer Supplementary Table 1 for primers) ...
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bioRxiv - Genomics 2020Quote: ... PCR amplified and cloned into Esp3I-digested plentiGuide-Puro (Addgene plasmid ID: 52963) lentiviral vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... the natNT2 marker was PCR amplified from pUG-natNT2 (Addgene plasmid #110922, (26)) using primers 10297 and 10298 ...
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bioRxiv - Neuroscience 2019Quote: ... the iGluu gene was amplified by PCR from pCI-syn-iGluu (Addgene #106122) using Phusion polymerase (forward 5’-CATCAGGATCCATGGAGACAGACACACTCC-3’ ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
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bioRxiv - Bioengineering 2021Quote: ... Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966) as a template and cloned together with either pJ23119 or pAtU6 for sgRNA expression.
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bioRxiv - Cell Biology 2021Quote: The KIF18B coding sequence was amplified by PCR from 24xMoonTag-kif18b-24xPP7 (Addgene plasmid #128604 [38] was a gift from Marvin Tanenbaum ...
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bioRxiv - Neuroscience 2019Quote: ... nGFP was PCR amplified from pAd nGFP (Addgene, 19413, deposited by Douglas Melton) using primers (forward 5’-CATCATCGAT ATGGTGCACGTGGATCCAA −3’ ...
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bioRxiv - Biophysics 2022Quote: ... Kinesin 1 1-401 (K401) was PCR amplified from pWC2 plasmid (Addgene # 15960). Ncd 236-701 was PCR amplified from a plasmid gifted by Andrea Serra-Marques.
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bioRxiv - Microbiology 2020Quote: The SNAP-tag gene was PCR amplified from pSNAP-tag(m) (Addgene #101135) and cloned into pVpr-IN.eGFP(Albanese et al. ...
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bioRxiv - Microbiology 2021Quote: ... The Cas9 part was generated via PCR amplification of pCas9/Decoy (Addgene #80324)8 using primers P12/P13 ...
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bioRxiv - Molecular Biology 2019Quote: ... The ERT2 DNA fragment was PCR amplified from pCAG-Cre-ERT2 (Addgene #14797) using 1Fw and 1Rv primers and inserted between SalI and BamHI sites of pBudCE4.1-3xHA ...
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bioRxiv - Immunology 2020Quote: ... The mAID-Clover cassette was PCR amplified from the plasmid pMK290 (Addgene, #72828). These fragments together with a puro selection cassette amplified from pEN84 were assembled in an order diagramed in Extended Data Fig ...
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bioRxiv - Systems Biology 2021Quote: ... individual drivers were PCR amplified out of the Cancer Pathways kit (Addgene #1000000072)21 ...