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Citations for Addgene :
301 - 350 of 1451 citations for Recombinant Human PRMT5 Flag & His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Full length FOXN1 cDNA without a stop codon was cloned into vector backbones positioning at its C-terminus either a flag (pCSF107mT-GATEWAY-3’-FLAG, Addgene), myc (pCSF107mT-GATEWAY-3’-Myc tag ...
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bioRxiv - Genetics 2020Quote: pDEST-FLAG-HA-USP5 and pDEST-FLAG-HA-OTUD5 were gifts from Wade Harper (Addgene plasmids # 22590 and # 22610(42). OTUD5 patient mutations (G494S ...
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bioRxiv - Cell Biology 2023Quote: ... To generate KEAP1-FLAG mutants, single and double point mutations (C151S/A, K131A) were introduced into FLAG-KEAP1 (Addgene #28023) using primers synthesized by IDT and a Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... and pQCXIH-Flag-YAP-S381A (Addgene plasmid, #33068)(Zhao et al. ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV-hSyn-Flpo-3×FLAG (no. 173047, Addgene), which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629 ...
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bioRxiv - Molecular Biology 2019Quote: ... the FLAG-TPR-pcDNA3.0 plasmid (Addgene plasmid # 60882) (61 ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-LacZ (ORF originated from Addgene #25893), were subcloned into SmaI (NEB #R0141 ...
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bioRxiv - Cancer Biology 2020Quote: ... and XE251-pcDNA3.1 (zeo) FLAG-hDsh3 (Addgene #16758) respectively ...
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bioRxiv - Cell Biology 2020Quote: ... pRK7-FLAG-Rheb vector21 was purchased from Addgene and was used to generate Rheb mutants via site-directed mutagenesis ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product and pCS2-FLAG (AddGene 16331) were digested with EcoRI and XhoI and ligated with T4 DNA Ligase ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA3 FLAG-TPR was acquired from Addgene (60882). pcDNA6.2 NXF1 was generated by Gateway cloning pENTR NXF1 (DNASU ...
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bioRxiv - Synthetic Biology 2020Quote: ... a C-terminal FLAG tag (pICSL50007, Addgene #50308), and a 3’ untranslated region and terminator sequence (3UTR ...
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bioRxiv - Cancer Biology 2020Quote: ... and pcDNA3-neo-Strep-flag-Cterm (#102645, Addgene) vectors to generate constructs encoding (hemagglutinin [HA])-tagged Decorin and E-Cadherin ...
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bioRxiv - Biochemistry 2021Quote: ... pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG (Addgene # 74261); pCMV6-XL4 ASXL1 (1-479 ...
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bioRxiv - Cancer Biology 2021Quote: FLAG-TRIM67 was cloned into pLVX-puro (Addgene) using appropriate restriction enzyme sites ...
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bioRxiv - Neuroscience 2022Quote: ... FLAG-FUS WT vector backbone (Addgene no. 44985) was digested with KpNI and XhoI enzymes and the FUS CDS is replaced by ER81 CDS which was amplified using mouse OB cDNA.
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bioRxiv - Molecular Biology 2023Quote: ... or IntS12 (pMtnA FLAG-IntS12 puro, Addgene #195077) under the control of the Metallothionein A promoter were generated using the previously described pMT FLAG MCS puro plasmid.26 The reporter plasmid expressing eGFP downstream of the U4:39B snRNA gene was previously described.41 An analogous reporter expressing eGFP downstream of U5:34A snRNA (Hy_U5:34A eGFP SV40 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti-TetONE-FLAG-Puro were synthesized by Addgene and used to generate E0771.lmb.PuroR cell line ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3-FLAG-DDB1 was obtained from Addgene (#19918). SR-4835 was purchased from MedChemExpress and CU-0904 ...
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bioRxiv - Molecular Biology 2023Quote: ... The C-FLAG-pcDNA3 expression vector (Addgene 20011) was also used as a negative control for FLAG-tagged ZDHHC expression ...
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bioRxiv - Physiology 2023Quote: FLAG-IKK2 kinase inactive K44M (IKK2DN, Addgene, #15466) was cloned into pLenti-CMV-Puro DEST vector (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... pN3-3×Flag-Control was purchased from Addgene (Watertown ...
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bioRxiv - Biochemistry 2024Quote: ... mMib1-Flag was purchased from Addgene (catalog #37116).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with tandem mRFP-GFP fluorescent-tagged LC3 (ptfLC3) (Addgene #21074). 24h post-transfection ...
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bioRxiv - Immunology 2019Quote: ... Expression plasmids encoding EGFP-tagged Rab5a and its mutants were purchased from Addgene, USA ...
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bioRxiv - Physiology 2019Quote: ... GFP-PGC1 plasmid expressing eGFP-tagged mouse PGC1a was acquired from Addgene(50). For in vivo electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2019Quote: ... Histidine-tagged PKA catalytic subunit (a gift from Susan Taylor, Addgene plasmid #14921) and PKI3 were previously described ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-tagged LAMP1 was a gift from Amy Palmer (Addgene plasmid # 45147). The myc-tagged WWOX plasmid was kindly provided by R ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged SQSTM1 and RFP-LC3 were gifts from Qing Zhong (Addgene, 28027)20 and Tamotsu Yoshimori (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: A pET28b plasmid encoding C-terminal 6xHis-tagged nuclear Pif1 (Addgene plasmid #65047)64 was employed to express and purify Pif1 from E ...
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bioRxiv - Cancer Biology 2024Quote: ... HA or myc-tagged cDNAs were from Addgene (66851, 66852, 32834, 32836, 32839) or synthesized (IDT) ...
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bioRxiv - Cancer Biology 2022Quote: ... human E2F5 and human DP1 were purchased from Addgene, whereas the ORF encoding human p130 from Origene.
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bioRxiv - Cancer Biology 2021Quote: HEK293T cells were transfected with plasmids encoding for Flag-p105 or Flag-p27 together with the plasmid HA-Ubiquitin (Addgene #18712). After 24h ...
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bioRxiv - Microbiology 2020Quote: ... pcDNA-FLAG-Ulk1 (Plasmid # 27636) 62 and pcDNA4-VPS34-FLAG (Plasmid # 24398) 63 were purchased from Addgene (Cambridge, MA 02139, USA). Myc-Msr1 was constructed by inserting Msr1 ORF (amplified from Origene clone MR205384 ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...
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bioRxiv - Immunology 2022Quote: ... pVitro-hygro-N-myc-hVps34/Vps15-C-V5-his-plasmid (Addgene #24055), pEGFP-Atg14L (Addgene #21635 ...
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bioRxiv - Biochemistry 2022Quote: pQE-60 roGFP2-His was a gift from Tobias Dick (Addgene #65046)(50) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid for expression of EPS15-GFP-His was obtained from Addgene (#170860). EPS15D177S was generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Immunology 2019Quote: ... DNA sequence encoding microRNA-722 tagged with Dendra2 were amplified from (Addgene plasmid # 97163) with the following primers ...
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bioRxiv - Cancer Biology 2020Quote: The vector for V5-tagged active WNT11 was a gift from Xi He (Addgene plasmid #43824 ...
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bioRxiv - Cancer Biology 2022Quote: ... pLV-ER GFP encoding GFP tagged SEC61β was a gift from Pantelis Tsoulfas (Addgene plasmid #80069 ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
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bioRxiv - Cell Biology 2019Quote: ... cells expressing GFP-tagged endogenous CLIC4 were transfected with Cry2PHR-mCH-RhoA (Addgene #42959). Cells were kept in the dark and incubated for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151) ...
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bioRxiv - Molecular Biology 2022Quote: ... The ER was marked using a Cytochrome p450 (CytERM) tagged to mScarlet (Addgene #85066), which was utilized as an ER marker ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... A cDNA for GFP-tagged mouse septin 6 was obtained from Addgene (plasmid #38296) and cloned into the pLVX-IRES-puro vector (Clontech) ...