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Citations for Addgene :
501 - 550 of 1451 citations for Recombinant Human PRMT5 Flag & His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: pAd-Track FLAG-SIRT1 was a gift from Pere Puigserver (Addgene plasmid # 8438). pAdtrack CMV plasmid was a gift from Bert Vogelstein (Addgene plasmid # 16405) ...
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bioRxiv - Biochemistry 2021Quote: ... pFRT/TO/FLAG/HA-DEST PABPC4 was from Thomas Tuschl (Addgene plasmid #19882) (Landthaler ...
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bioRxiv - Neuroscience 2021Quote: ... Flag-HA-HuD was obtained by cutting the pFRT-TODestFLAGHA_HuD plasmid (Addgene, #65757) with PaeI and BglII enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... HDAC6-FLAG (#13823) and pEF5B-FRT-GFP-aTAT1 (#27099) were purchased from Addgene. Tub-GFP-HDAC6 was made by PCR amplifying the HDAC6 sequence from the HDAC6-FLAG construct and ligating the PCR amplicon into the Tub-GFP construct ...
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bioRxiv - Cell Biology 2022Quote: The coding sequence of Mettl3 was amplified from pcDNA3/Flag-METTL3 (Addgene, #53739) and inserted into the lentiviral plasmid pLOXCMV (Qi et al ...
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bioRxiv - Physiology 2022Quote: ... FLAG-IKKβ-S177E/S181E (#11105) and HA-Ubiquitin (#18712) were acquired from Addgene. HA-TRAF6 was generated by cloning the TRAF6 fragment into pcDNA3-HA plasmid ...
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Histone H3K36me2-specific methyltransferase ASH1L is required for the MLL-AF9-induced leukemogenesisbioRxiv - Cancer Biology 2021Quote: The pMIG-FLAG-MLL-AF9 retroviral vectors as obtained from Addgene (Plasmid #71443). Retroviral vectors were generated by co-transfection of retroviral vectors with pGag-pol ...
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bioRxiv - Cancer Biology 2021Quote: ... The pcDNA5-FRT/TO-LacR-FLAG-TOPBP1 plasmid was obtained from Addgene (#31313). Point mutants were introduced by site-directed mutagenesis using Quikchange (Agilent) ...
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bioRxiv - Developmental Biology 2019Quote: ... pcDNA-HDAC6-FLAG was a gift from Dr.Tso-Pang Yao (Addgene plasmid # 30482).
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bioRxiv - Molecular Biology 2019Quote: ... Flag-PIAS4 WT plasmid was a gift from Ke Shuai (Addgene plasmid # 15208). RNF4-Myc-FLAG WT plasmid was purchased from OriGene (CAT# ...
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bioRxiv - Cell Biology 2019Quote: ... The pcDNA3-Flag-Apex-Nes was a gift from Alice Ting (Addgene, 49386). The N1-mTurquoise2 (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... A plasmid expressing anti-CRISPR protein (pEJS581; pCSDest2-AcrIIC4Hpa-FLAG-NLS; Addgene # 113436) was included in the triple-transfection packaging process to maintain intact rAAV:HDR:cleaved plasmids during production ...
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bioRxiv - Cell Biology 2021Quote: ... pcDNA-HDAC6-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30482) (Kawaguchi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... K562 or HeLa cells were transfected with pEBB-Flag-GCN5 (Addgene, Plasmid #74784) or an empty vector using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: lenti SYN-FLAG-dCas9-KRAB-MeCP2 was a gift from Jeremy Day (Addgene plasmid # 155365 ...
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bioRxiv - Cancer Biology 2022Quote: ... pENTR4-FLAG (w210-2) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17423 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG-β-catenin K19R (Plasmid #Addgene plasmid # 44749; http://n2t.net/addgene:44749; RRID:Addgene_44749), FLAG- β-catenin K19R/K49R(Addgene plasmid #44751 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG- β-catenin K19R/K49R(Addgene plasmid #44751; http://n2t.net/addgene:44751; RRID:Addgene_44751)) ...
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bioRxiv - Cell Biology 2023Quote: ... and N131-N160 was inserted using the pEXPqcxip-hCCDC47-FLAG vector (Addgene, 159141) as a template and primers designed to include a C-terminus MYC-tag ...
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bioRxiv - Immunology 2024Quote: ... pcDNA3-HA-RIPK3 (78804) and pcDNA3-FLAG-RIPK1 (78842) were procured from Addgene.
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bioRxiv - Immunology 2021Quote: Recombinant HA (rHA) proteins were expressed using the pcDNA 3.1+ plasmid (Addgene, Watertown, MA). Each HA gene was truncated by removing the transmembrane (TM ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant Lentiviral Vector encoding Myoc Y437H was constructed from plasmids pLentCMV-GFP(Addgene 17448)(Campeau et al. ...
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bioRxiv - Cell Biology 2020Quote: Plasmids for the expression of recombinant α-Syn were: pT7-7 α-Syn (Addgene plasmid #3604636 ...
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bioRxiv - Neuroscience 2022Quote: ... The recombinant AAV vector was pseudotyped with AAV5 capsid protein and packaged by Addgene.
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bioRxiv - Cancer Biology 2023Quote: ... The recombinant plasmids were packaged into the lentivirus with pSPAX2 (Addgene, 12260, RRID: Addgene_12260) and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... The recombinant plasmids were packaged into the lentivirus with pSPAX2 (Addgene, 12260, RRID: Addgene_12260) and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: The lentiviral construct containing a truncated version of 53BP1 tagged with mApple was a gift from Ralph Weissleder (Addgene plasmid # 69531 ...
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bioRxiv - Neuroscience 2020Quote: ... Dphox and phox vectors were C-terminally tagged with GFP by infusion cloning in frame into CAG-GFP (Addgene) using the following primers ...
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bioRxiv - Neuroscience 2022Quote: ... The AU1-tagged wild-type and two mutant (S2215Y and R2505P) mTOR constructs were gifts from Fuyuhiko Tamanoi (Addgene) 59 ...
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272; http://n2t.net/addgene:27272; RRID: Addgene_27272) (Amin et al. ...
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bioRxiv - Neuroscience 2022Quote: ... A retrograde GFP-tagged adeno-associated virus rAAV2/1-retro (retrograde AAV-CAG-GFP; serotype “retro”, Addgene, Cat. # 37825) was pressure injected into M2 (170 ...
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bioRxiv - Molecular Biology 2023Quote: All-in-one lentiviral plasmids for Dox-inducible expression of UNK without GFP in HeLa cells were created by insertion of the Flag-HA-tagged full-length WT or mutant UNK amplified from the corresponding entry vector into the pLIX_403 plasmid (Addgene_41395) between NheI and AgeI sites ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... A viral vector containing a Cre expressing cassette (pAAV-CMV-HI-eGFP-Cre-WPRE-SV40, Addgene; #105545) was used to induce Fkbp5 deletion in Fkbp5lox/lox mice ...
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bioRxiv - Biochemistry 2020Quote: hnRNPA2 LC (190-341), insoluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98657)
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bioRxiv - Biochemistry 2022Quote: AR-LBD (663-919) containing an N-terminal His-tag and encoded in pET15b plasmid (Addgene #89083) was expressed in Rosetta (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... 6 × 107 RPE1 cells were infected with the human sgRNA library (Human GeCKO v2 Library Cat#1000000048, Addgene) at an MOI of ∼0.3 ...
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bioRxiv - Biochemistry 2019Quote: ... and a mammalian expression vector pcDNA3 encoding hemagglutinin tagged constitutively active glycogen synthase kinase-3β (GSK-3β) (S9A) (pcDNA3-GSK3β, # 14754, RRID: Addgene_14754). The EGFP in pRK5 vector was first replaced by iRFP to construct an iRFP tagged WT tau by golden gate assembly (pRK5-iRFP-tau ...
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bioRxiv - Cancer Biology 2021Quote: ... and after selection in 300 μg/ml G418 cells were tagged with luciferase by transduction with lentiviruses expressing pFU-Luc2-eGFP (Addgene).
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bioRxiv - Cancer Biology 2021Quote: ... GFP and GFP-tagged K19 WT and mutantconstructs were cloned from pEGFP-C3 K19 constructs into pLenti CMV Hygro (plasmid #17484. Addgene) using the primers listed in Table 1 with the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Cell Biology 2019Quote: ... cells expressing GFP alone or pLVX:GFP-CLIC4 were lysed and proteins were immunoprecipitated using a GST-tagged GFP-nanobody (Addgene #61838) that was covalently linked to Affigel 10/15 resin (BioRad) ...
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bioRxiv - Microbiology 2019Quote: ... were chromosomally tagged with YFP using the mini-Tn7 system 41 (Plasmid pUC18T-mini-Tn7T-Gm-eyfp and pTNS1, Addgene plasmid # 65031 ...
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bioRxiv - Cell Biology 2020Quote: ... Green fluorescent protein-tagged HIF-1α DM(P402/564A) was generated by inserting the sequence of Clover (Addgene plasmid #40259) behind the myc-tag in the BamHI-digested pCMV-Myc-HIF-1α DM(PP/AA ...
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bioRxiv - Biochemistry 2023Quote: Dephosphorylated AurA (residues 122-403, TEV-cleavable, N-terminal His6-tagged, kanamycin-resistance) in pET28a and LPP (#79748) from Addgene were cotransformed in BL21(DE3 ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA for expressing CRY2olig tagged with mCherry at its C-terminus was obtained from Addgene (#60032; Watertown, MA, USA). The plasmid (1.0 µg/dish ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid expressing HA-tagged CK2α (K68M) was subcloned from pGV15-CK2α-K68M (a gift from David Litchfield, Addgene plasmid 27089) into SalI and NotI restriction enzyme sites in the pCMV-HA vector.
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bioRxiv - Cell Biology 2022Quote: pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A was a gift from Nicolas Manel (Addgene plasmid # 86674 ...
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bioRxiv - Cell Biology 2019Quote: ... pFRT/FLAG/HA-DEST QKI was a gift from Thomas Tuschl (Addgene plasmid #19891)56 ...
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bioRxiv - Molecular Biology 2019Quote: ... RACK1-FLAG was cloned into pLenti CMV Puro DEST (w118-1) (Addgene plasmid #17452) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and pBa-EGFP-flag-BicD2594-FKBP (Addgene plasmid #63569; http://n2t.net/addgene:63569; RRID:Addgene_63569), pBa-FRB-3myc-KIF13A tail 361- 1749 (Addgene plasmid #64288 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-mNep2-FLAG (mNeptune2-N1, a gift from Michael Davidson (Addgene plasmid # 54837 ...