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Citations for Addgene :
301 - 350 of 707 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... we used stereotactically targeted virus injections of rAAV S1 FLEX-CAG-jGCaMP7s-WPRE (Lot v28549, Addgene, #104495 AAV-1) into the MSDB of 3— 6 months old ChAT-Cre mice ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding oligonucleotides (Table S1) were annealed and cloned in the pU6-BbsI-chiRNA vector linearized with BbsI (Addgene plasmid #45946 ...
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bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Developmental Biology 2020Quote: ... Bot oligo – 5’ aaacGGGTGAGACCCATGTATTTc3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
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bioRxiv - Cancer Biology 2023Quote: ... and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella)67 for expressing the mutant Cyclin E ...
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bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032) 39 ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenised and barcoded spike sequence templates were then added into MluI and XbaI digested lentivirus backbone (Addgene #204579) using HiFi reaction (NEB E2621L) ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... The plasmid encoding Control-TAG is available on Addgene (Addgene #138209, pcDNA3.1- (empty)-TAG) ...
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bioRxiv - Cell Biology 2021Quote: ... for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version.
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bioRxiv - Cancer Biology 2022Quote: ... maintaining the DDK-MYC tag and then into Pentr4FLAG (Addgene Cat. no. 17423) before cloning into the lentiviral DOX-inducible destination vector pLIX403 (Addgene Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 10 pmol of PCR repair template amplified from pSNAP-tag plasmid (Addgene #101135) or mTagBFP2 plasmid (Addgene #75029 ...
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bioRxiv - Molecular Biology 2024Quote: ... The SMASh tag was removed and replaced with 2xHA dTAG derived from Addgene plasmid 9179224 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli cells containing the plasmid pCAS were obtained from Addgene (#60847). Plasmid pCAS (8.7 Kb ...
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bioRxiv - Biochemistry 2022Quote: ... coli NiCo21(DE3) cells were prepared and transformed with pMSP2N2 (Addgene) using a standard New England BioLabs (NEB ...
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bioRxiv - Immunology 2022Quote: ... coli transduced with a SpyCatcher003-mi3 expression plasmid (Addgene, Watertown, MA) were lysed with a cell disrupter in the presence of 2 mM PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180 ...
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bioRxiv - Developmental Biology 2023Quote: ... coli colonies with the pET28a-EGFP-CNA35 gene (Addgene, Plasmid #61603) were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were transformed with arabinose inducible EGFP-pBAD plasmid (Addgene #54762) overnight at 37°C ...
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bioRxiv - Genomics 2019Quote: ... A guide RNA sequence with a suitable protospacer adjacent motif (PAM) was found nearby the excision site (Figure 1A, Table S1) and cloned into gRNA_Cloning Vector (Addgene 41824)17 ...
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bioRxiv - Genomics 2019Quote: ... PCR products corresponding to each crRNA were amplified with U6 forward primer and corresponding antisense oligos (as listed in Supplemental Table S1) from the pX330 plasmid (Cat. 42230, Addgene). After digestion with DpnI (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, single guide RNAs (sgRNAs, designed using crispr.mit.edu, listed in Table S1) were cloned into pX458 vector (Addgene plasmid #48138) and transfected into H1299 cells ...
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bioRxiv - Biochemistry 2021Quote: ... sgRNAs in Table S1 were cloned in pSpCas9(BB)-2A-GFP (PX458, a gift from Feng Zhang, Addgene plasmid #48138) and transfected into mESCs ...
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bioRxiv - Cancer Biology 2023Quote: ... Enpp1 guide sequences listed in Table S1 were cloned into the BbsI site of PX458-GFP or P458-mCherry backbones (synthesized by Addgene) following the protocol from Ryuji Morizane Lab at Harvard in order ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides corresponding to designed guide RNAs (gRNA, listed in Table S1) were ligated into an entry vector pMpGE_En03 (Addgene #71535) and then transferred to a destination vector pMpGE010 (Addgene #71536).
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bioRxiv - Bioengineering 2019Quote: ... TGFB1-bio-His (proTGFβ) which was a gift from Gavin Wright (Addgene plasmid # 52185) [17] and HA-OVOL2 (OVOL2 ...
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bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
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bioRxiv - Biochemistry 2022Quote: ... pET29b-IPP1-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124137). 15µL of Lemo21(DE3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-E-Syt1 was a gift from Pietro De Camilli (Addgene plasmid # 66830) (41) ...
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bioRxiv - Developmental Biology 2023Quote: ... Bot oligo – 5’-aaacTTGGCACTCCATTAGATCCG-3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Biophysics 2024Quote: ... MDCK -wildtype or -transformed cells were transfected with E-cadherin-GFP plasmid (Addgene) using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and a plasmid encoding the spike protein (with a C-terminal truncation) from either SARS-CoV (Addgene cat 170447), SARS-CoV-2 53 ...
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bioRxiv - Immunology 2023Quote: The expression construct used to generate SARS-CoV-2 spike protein was a gift from Jason McLellan (Addgene #154754). Spike protein was prepared as previously described in20 ...
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bioRxiv - Immunology 2022Quote: ... and a 1xHA tag was created using pSL1394 as a base plasmid (Addgene # 129522). A homology-directed repair (HDR ...
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bioRxiv - Microbiology 2020Quote: ... was replaced with PspCas13b with HIV NES and HA tag (from pC0046 (Addgene #103862)) to generate pC0068-PspCas13b-HA for PspCas13b-HA purification.
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bioRxiv - Genomics 2022Quote: ... and either pENN.AAV.TBG.PI.N-FLAG-tag mSTAT5bCA.WPRE.bGH (see above) or pENN.AAV.TBG.PI.ffLuciferase.RBG (firefly luciferase expressed from TBG promoter; Addgene # 105538). Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... into the TfR-sfGFP-myc tag-SpyCatcher003 plasmid (Keeble et al. [55]. Addgene #133451) through Gibson Assembly (primers listed in Table S3) ...
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bioRxiv - Microbiology 2021Quote: ... SpyCatcher/Spy-tag and SUMO containing plasmids were purchased from Addgene (#133449 and #111560).
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bioRxiv - Biochemistry 2021Quote: ... and pGAT2 (N-terminal polyhistidine and GST tags; Addgene #112588; last three genes (61)) by Twist Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...