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Citations for Addgene :
251 - 300 of 667 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... coli cells using the plasmid pRK793 (Addgene, Plasmid # 8827)55 ...
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bioRxiv - Genomics 2021Quote: ... coli containing pTXB1-3xFlag-pA-Tn5-FL (Addgene, #124601), a kind gift from (Kaya-Okur et al. ...
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bioRxiv - Cell Biology 2019Quote: We generated Schizosaccharomyces pombe strains with SNAP-tag (Addgene plasmid 87024) or mEGFP (Addgene plasmid 87023 ...
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bioRxiv - Biochemistry 2023Quote: ... or N-terminal His10-MBP Tag (2CT-10; Addgene Plasmid #37237) were chosen as the MBP tag has been shown to increase the solubility of select proteins [9] ...
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bioRxiv - Plant Biology 2023Quote: ... a C-terminal 3×FLAG® epitope tag (pICSL50007; Addgene #50308), and 3′ UTR and terminator sequences from Agrobacterium tumefaciens nopaline synthase (AtuNOST ...
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bioRxiv - Cell Biology 2022Quote: ... The dual-tag Galectin3 plasmid was acquired from Addgene (ref. 64149). The TBC1D15 coding sequence was subcloned into vectors pEGFPC1 and pGEX6P1 for expression in mammalian and bacterial cells ...
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bioRxiv - Immunology 2021Quote: ... with pcDNa3.1 vector expressing SARS-CoV-2 spike (BEI repository) and the helper plasmid pSPAX2 (Addgene). The VSV-G and empty lentiviruses were produced by replacing pCDNA3.1-Spike with pcDNA3.1-VSV-G or pCDNA3.1 empty vector ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1-SARS2-Spike (2) and pcDNA3.1-hACE2 (2) were obtained from Addgene (Addgene plasmids #145032; #145033); pCI-SARS2-Spike expressing a codon optimized version of the gene coding for S protein of SARS-CoV-2 was provided by Nicolas Escriou (Institut Pasteur) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 whole spike protein HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754). SARS-CoV-2 HexaPro was expressed in Expi293F cells and purified with Ni-NTA resin followed by size exclusion as described previously [61] ...
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bioRxiv - Cancer Biology 2022Quote: The pLentiCRISPR E plasmid (Addgene, 78852, a gift from Phillip Abbosh) was modified with different selectable marker genes ...
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bioRxiv - Cancer Biology 2024Quote: ... The lentiviral E-cadherin-EGFP were generated from pCS-CG (Addgene) for E-cadherin knock-in of MDA-MB-231 as previously described [58] ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...
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bioRxiv - Immunology 2022Quote: ... pVitro-hygro-N-myc-hVps34/Vps15-C-V5-his-plasmid (Addgene #24055), pEGFP-Atg14L (Addgene #21635 ...
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bioRxiv - Biochemistry 2022Quote: pQE-60 roGFP2-His was a gift from Tobias Dick (Addgene #65046)(50) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid for expression of EPS15-GFP-His was obtained from Addgene (#170860). EPS15D177S was generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... and the SLC35A1 gene were designed using CRISPR direct (https://crispr.dbcls.jp/; S1 Table) and each sequence was cloned into plentiCRISPR plasmids [37] (Addgene plasmid #52961 ...
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bioRxiv - Cancer Biology 2023Quote: ... using pLenti_mENPP1_fwd and pLenti_mENPP1_rev primers in Table S1 and inserted into the XbaI-BamHI sites of pLenti-CMV-GFP-Puro (Addgene). To clone pLenti-CMV-mENPP1-T238A-GFP-Puro plasmid ...
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bioRxiv - Plant Biology 2021Quote: ... coli strains containing pAC-BETA-At (Addgene plasmid no. #53288), pAC-ZETA (Addgene plasmid no ...
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bioRxiv - Bioengineering 2019Quote: ... and bioluminescent ilux pGEX(-) Escherichia coli was purchased from Addgene, a gift from Dr ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-CodonPlus (DE3) cells containing pET28a-MSP1D1delltaH5 vector (Addgene cat# 71714 was kindly purchased by Dr ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DHL708 (a gift from Johan Paulsson, Addgene plasmid # 98417) [8] for all insertions ...
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bioRxiv - Biochemistry 2023Quote: ... coli with lambda phosphatase spectinomycin-resistant plasmid (Addgene plasmid #79748). We used either wild-type ...
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bioRxiv - Biophysics 2023Quote: ... coli biotin ligase BirA from the T7 promoter (Addgene#109424) in E ...
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bioRxiv - Neuroscience 2021Quote: ... CalR-HaloTag-Snap-tag-KDEL (ER-localizing) or GCaMP6f (Addgene 100837-AAV1). On day 14-18 the neurons were incubated for at least 2 h with 1 μM of the corresponding MaPCa dyes containing 0.04% Pluronic-F127 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Biophysics 2022Quote: ... The His6-SUMO tag was subsequently cleaved with human SenP1 (Addgene #16356) 27 and separated on Ni2+-Histrap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: GST-tag human HP1⍺ was a kind gift from Naoko Tanese (Addgene plasmid # 24074 ...
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bioRxiv - Genomics 2023Quote: ... Mito-tag plasmids were based on pMXs-3XHA-EGFP-OMP25 (Addgene, #83356) and pMXs-3XMyc-EGFP-OMP25 (Addgene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.5 μg of SARS-CoV-2 Spike plasmid (a gift from Fang Li, Addgene plasmid #145032) using Lipofectamine 3000 transfection reagent (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-SCoV-2S (Spike envelope plasmid; Sinobiologicals – Cat #: VG40589-UT) and pLJM1-EGFP (reporter; Addgene plasmid #19319) in a ratio of 1:1:2 respectively ...
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bioRxiv - Genetics 2020Quote: ... we co-transfected pcDNA3.1-SARS2-Spike or pcDNA3.1-SARS2-SpikeD614G (see above) with pcDNA3.1-ACE2 (Addgene 1786) at a 1:1 ratio (Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032);34 this gene was cloned into a modified pcDNA 3.1 backbone (Clontech-Takara ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032).
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV S was cloned into pcDNA3.3 vector from pcDNA3.1-SARS-Spike (Addgene plasmid#145031). SARS-CoV-2 N was cloned into a pSGC-his vector as described in our previous paper [17] ...
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bioRxiv - Developmental Biology 2020Quote: ... Bot oligo – 5’ aaacGGGTGAGACCCATGTATTTc3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Cancer Biology 2023Quote: ... and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella)67 for expressing the mutant Cyclin E ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
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bioRxiv - Cell Biology 2022Quote: ... guide RNAs targeting a gene of interest (Table S1) were cloned into pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene plasmid #50946), using the BbsI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs (Table S1) were designed using an online CRISPR design tool (crispr.mit.edu) and cloned into a gRNA cloning vector (Addgene, 41824) following option B from the gRNA Synthesis Protocol(53) ...
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bioRxiv - Neuroscience 2022Quote: ... we used stereotactically targeted virus injections of rAAV S1 FLEX-CAG-jGCaMP7s-WPRE (Lot v28549, Addgene, #104495 AAV-1) into the MSDB of 3— 6 months old ChAT-Cre mice ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding oligonucleotides (Table S1) were annealed and cloned in the pU6-BbsI-chiRNA vector linearized with BbsI (Addgene plasmid #45946 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...