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Citations for Addgene :
101 - 150 of 667 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Plasmids encoding the full-length spike proteins with native signal peptides were cloned into the background of the HDM-SARS2-Spike-delta21 plasmid (Addgene Plasmid #155130). This construct contains a 21 amino acid c-terminal deletion to promote viral expression ...
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bioRxiv - Immunology 2022Quote: The 21-amino acid C-terminally truncated spike proteins with native signal peptides were cloned in place of the HDM-SARS2-spike-delta21 gene (Addgene plasmid, 155130). This construct contains a 21-amino acid C-terminal deletion to promote viral production ...
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bioRxiv - Biochemistry 2023Quote: The 21-amino acid C-terminally truncated spike proteins with native signal peptides were cloned in place of the HDM-SARS2-spike-delta21 gene (Addgene plasmid, 155130). This construct contains a 21-amino acid C-terminal deletion to promote viral production ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-E-Syt1(Addgene #66830), EGFP-JPH3 and EGFP-VAP-B ...
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bioRxiv - Microbiology 2023Quote: ... M and E (Addgene 177938); and S (D614G N501Y ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Addgene Cat# 17827) bacterial culture obtained from overnight culture in LB broth (Luria Bertani broth ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10GFP (Addgene #109392), a strain constructed in the same way in the prior study of burden by Ceroni et al.21
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bioRxiv - Cancer Biology 2020Quote: ... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and SERPINE1-bio-his (Addgene #52077) which were gifts from Gavin Wright without signal sequence to prevent cleavage of fused proteins (75) ...
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bioRxiv - Developmental Biology 2021Quote: pET-28b-RfxCas13d-His (Addgene #141322) plasmid containing the T7 promoter was linearized by using NotI restriction site ...
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bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-BMAL1-His (AddGene CN: 31367), pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN ...
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bioRxiv - Developmental Biology 2023Quote: pcDNA3-BMAL1-His (AddGene CN: 31367) were purchased from the Addgene ...
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bioRxiv - Genetics 2020Quote: sgRNAs (Supplementary Table S1) were cloned into pLenti-Guide-Puro (Addgene) and delivered to hTERTimmortalized RPE-1 cells carrying a tetracycline-inducible promoter by lentiviral transduction ...
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bioRxiv - Neuroscience 2020Quote: ... AAV plasmids for DREADD viruses were purchased from Addgene (Table S1) and packaged at the European Molecular Biology Laboratory ...
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids used in this work (Table S1) were obtained from Addgene. All primers were synthesized by IDT with standard desalting ...
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bioRxiv - Cell Biology 2023Quote: ... pCMV-Tag/WT M87 (Addgene #89322), pCIG2-SNAP-M87 (generated for this study from pCIG2-GFP-MACF43 by replacing GFP with SNAP-tag from pSNAPf vector (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... MBP-tag was cloned from Addgene CAT#98651 and fusion constructs were assembled using 2xHiFi mastermix (NEB #E2621L ...
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bioRxiv - Biochemistry 2021Quote: ... spike was cloned into a pcDNA5-based vector (Addgene #113547) with the following additional N-terminal Ig-Kappa leader and C-terminal epitopes ordered as two separate gBlocks (IDT) ...
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bioRxiv - Biophysics 2020Quote: ... and 750 ng of Spike-18aa truncated (Addgene plasmid # 149541) were mixed ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Immunology 2021Quote: ... and pcDNA 3.1-SARS-CoV-2 Spike (Addgene plasmid #145032) for 72 hours at 37°C under 5% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... and David Nemanzee [32] (SARS-CoV Spike ΔC28 (Addgene #: 170447), Spike ΔC18 (Addgene # ...
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bioRxiv - Immunology 2024Quote: ... using the SARS-CoV1 SPIKE plasmid pcDNA3.3_CoV1_D28 (Addgene plasmid # 170447)39 ...
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bioRxiv - Neuroscience 2020Quote: ... engineered and optimized glycoprotein (oG) [76] sequence was ligated to pAAV-FLEX sequence from pAAV-FLEX-GFP (Addgene).
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bioRxiv - Biochemistry 2021Quote: ... GST-tag and MBP-tag were attached to its N- and C-terminus respectively (Addgene ID 169195). The cleavage at G//A resulted in 25 and 42 kDa products ...
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bioRxiv - Developmental Biology 2020Quote: ... The U6.3>gRNA.f+e (#99139, Addgene) vector was digested over night with BsaI-HF enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 (KIlacZ, Addgene: #52696) harboring BAC-pks was back-diluted 1:100 into fresh M9-CAS and mixed in a 1:1 ratio with a similarly back-diluted culture of lacZ− E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli Marionette DH10B (Addgene, #108251) was used to construct and characterize genetic circuits ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10B M.XmnI (Addgene, 114269)34 and plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... coli EC1000 strain94 (Addgene #71852), a kanamycin resistant strains carrying a single copy of the repA gene in the glgB gene ...
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bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ...
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bioRxiv - Cancer Biology 2024Quote: ... S1 Table) were annealed and cloned into the pLKO.1 vector (RRID:Addgene_8453) (29 ...
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bioRxiv - Cell Biology 2023Quote: ... A pair of 20-mer oligonucleotides targeting the 5’ end of the dtat coding sequence was ligated into pAc-sgRNA-Cas9 (Addgene) and validated by sequencing ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 spike or VSV-G (pMD2.G (Addgene #12259)) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Molecular Biology 2021Quote: ... and either 750 ng of Spike-18aa truncated (Addgene plasmid # 149541), or generated Spike variant plasmids (alpha ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T cells were transfected with Spike-18aa truncated (Addgene plasmid # 149541), or engineered Spike-variants of concern (Alpha ...
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bioRxiv - Immunology 2021Quote: ... myc (pCSF107mT-GATEWAY-3’-Myc tag, Addgene), green fluorescence protein (GFP ...
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bioRxiv - Developmental Biology 2022Quote: ... gRNA expression vectors U6.3> gRNA.f+e and U6.3> Control.gRNA.f+e were obtained from Addgene (Gandhi et al., 2017). Oligo DNAs for the gRNA templates were designed as follows (lowercase letters indicate BsaI overhang) ...
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bioRxiv - Immunology 2019Quote: ... were incorporated into two complementary 100-mer oligonucleotides and cloned into a gRNA containing plasmid containing the (NeoR/KanR) cassette (Addgene # 41824). The human codon optimized pCAGGS-Cas9-mCherry was used for gene-editing experiments (a gift from Stem Cell Core Facility at Columbia University) ...
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bioRxiv - Bioengineering 2023Quote: ... The AAV capsid AAV1-X1 was built by inserting a 7-mer peptide between AAs 588-589 of the AAV1 cap gene in AAV1-Rep-Cap (Challis et al. 2019) (Addgene 112862).
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral packaging plasmid psPAX2 and pMD2.G encoding the vesicular stomatitis virus glycoprotein were gifts from Didier Trono (Univ. of Geneva) and purchased from Addgene (http://n2t.net/addgene:12260 and http://n2t.net/addgene:12259 ...
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bioRxiv - Immunology 2020Quote: ... pCMV-VSV-G plasmid encoding for the vesicular stomatitis virus glycoprotein was a gift from Bob Weinberg (Addgene plasmid #8454). LeGO-G2 ...
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bioRxiv - Molecular Biology 2019Quote: ... MmEsco2-myc/his and H2B-mCherry were amplified from the vectors pcDNA3.1/myc-His and pcDNA3-H2B-mCherry (Addgene, 20972), respectively ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The CyPet gene was amplified by PCR from the pCyPet-His vector (pCyPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14030 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The YPet gene was amplified by PCR from the pYPet-His vector (pYPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14031 ...
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bioRxiv - Developmental Biology 2019Quote: ... CrispR guides were made by cloning annealed oligos (Table S1) into px458 (Addgene). 2μg of vector DNA were transfected into 8×105 ESCs using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... carrying a previously constructed helper plasmid (24) (pDY118A, Figure S1, Addgene ID 182958). pDY118A encodes SsrA-tagged SpCas9 (10 ...
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bioRxiv - Molecular Biology 2020Quote: ... coli C321.ΔprfA EXP11 (Addgene #48998). For stop codon suppression by genetic code expansion ...
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bioRxiv - Biophysics 2021Quote: ... coli from pet30b-7M SrtA (Addgene plasmid # 51141 was a gift from Hidde Ploegh ...