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Citations for Addgene :
251 - 300 of 686 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg pSMPUW-IRIS-Neo-H2BmRFP (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying CRISPR-Cas9 constructs were produced using HEK293 cells with the following plasmids: pSS172 (pMD2.G, Addgene #12259), pSS173 (psPAX2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The PDE6D scan library contains 116 unique sgRNA was packaged by HEK293 cells (ATCC) co-transfected with psPAX2 (Addgene) and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R cells were co-transfected with an AP2-HA plasmid (μ2-HA-WT; Addgene plasmid # 32752 ...
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bioRxiv - Developmental Biology 2023Quote: ... viral lenti-particles were generated by transfecting HEK293 cells in a T25 flask with 15 μg of the lentiviral WβS-reporter vector (7TGC; Addgene #24304 ...
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bioRxiv - Microbiology 2023Quote: ... hGM-CSF and hIL-4 were produced from HEK293 cells stably transduced with pAIP-hGMCSF-co (Addgene no. 74168) or pAIP-hIL4-co (Addgene no ...
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bioRxiv - Molecular Biology 2024Quote: ... Parental HEK293 cells were co-transfected with 1.0 µg each of PBKS-Cas9-2A-eGFP plasmid (Addgene plasmid #68371) and plasmid encoding for gRNA ...
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bioRxiv - Microbiology 2024Quote: ... pseudovirus constructs (PV) have been developed by three-plasmid co-transfection in HEK293 cells using lentiviral backbone (Addgene # 8455), firefly luciferase reporter (Addgene #170674 ...
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bioRxiv - Cell Biology 2019Quote: pFRT/FLAG/HA-DEST QKI (#19891) was purchased from Addgene and was subsequently cloned into a pCDH1-MSCV-EF1α-GreenPuro vector (SBI) ...
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... USP7 expression vector pCI-neo-Flag backbone (Addgene Plasmid # 16655). All the constructs were verified by restriction digestion and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... and pIRESneo-FLAG/HA EYFP (Addgene plasmid # 10825, FH-EYFP) were gifts from T ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-STX17 was a gift from Noboru Mizushima (Addgene #45911) and mCherry-DFCP1 was a gift from Do-Hyung Kim (Addgene #86746).
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bioRxiv - Cell Biology 2021Quote: ... HDAC4 Flag (Addgene plasmid # 13821; http://n2t.net/addgene:13821; RRID:Addgene_13821), HDAC5 Flag (Addgene plasmid # 13822 ...
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bioRxiv - Cell Biology 2021Quote: ... HDAC3 Flag (Addgene plasmid # 13819; http://n2t.net/addgene:13819; RRID:Addgene_13819), HDAC4 Flag (Addgene plasmid # 13821 ...
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bioRxiv - Cell Biology 2021Quote: ... HDAC5 Flag (Addgene plasmid # 13822; http://n2t.net/addgene:13822; RRID:Addgene_13822), and HDAC7 (Addgene plasmid # 13824 ...
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bioRxiv - Immunology 2020Quote: ... and cloned into FLAG-HA-pcDNA3.1 vector (Addgene, plasmid #52535) using Gibson assembly kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and 125 ng expression plasmid (pcDNA3-FOXO::FLAG, Addgene #13507) using manufacturer’s recommendations for lipofectamine 3000 ...
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bioRxiv - Genetics 2019Quote: FLAG tagged ubiquitin was cloned in pCW57.1 vector (Addgene # 41393) between BsrG1 sites and verified through sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... and β-arrestin 1-FLAG (#14687) were obtained from Addgene.
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bioRxiv - Cell Biology 2021Quote: ... The plasmids ordered were SIRT1 Flag (Addgene plasmid # 13812; RRID:Addgene_13812), SIRT2 Flag (Addgene plasmid # 13813 ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids ordered were SIRT1 Flag (Addgene plasmid # 13812; RRID:Addgene_13812), SIRT2 Flag (Addgene plasmid # 13813 ...
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bioRxiv - Immunology 2021Quote: ... pCMV14-3X-FLAG-SARS-CoV-2S was from Addgene (#145780) and was described earlier41 ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmids used include: 1436 pcDNA3 Flag HA (Addgene plasmid #10792) and pCMV3-human NEK2-Myc (HG10054-CM ...
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bioRxiv - Microbiology 2022Quote: ... we also expressed WT and D614 S-protein-FLAG (Addgene plasmids 156420 and 156421 ...
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bioRxiv - Neuroscience 2023Quote: ... into the backbone of pAAV CaMKII iRFP-FLAG (Addgene 149513) after digestion with NheI and AscI restriction enzymes (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... pCAG_smFP FLAG (Addgene plasmid # 59756; http://n2t.net/addgene:59756; RRID:Addgene_59756), and pCAG_smFP Myc (Addgene plasmid # 59757 ...
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bioRxiv - Molecular Biology 2023Quote: – LV_CMV-Flag-eIF4E (lentivirus of second generation; Addgene plasmid #38239).
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bioRxiv - Molecular Biology 2023Quote: ... The SOX4-FLAG construct was obtained from Addgene (catalog #110360). Expression of these constructs was confirmed by western blot (see SFig2 in (8)].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pCMV-FLAG LAP2 was a gift from Joan Massague (Addgene_15738). The oligonucleotides targeting enhancer regions (E9 ...
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bioRxiv - Physiology 2024Quote: ... and pcDNA3-Flag-FKHR-ΔDB-AAA (Addgene 9022, 9023, 10694) were kind gifts from Dr ...
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bioRxiv - Biochemistry 2024Quote: ... 2xStrep-Flag-FOXA1-HA was introduced to pINDUCER21 (Addgene #46948) to generate pINDUCER21_FOXA1 by Gateway cloning protocol (Thermo).
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bioRxiv - Cell Biology 2020Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol (Tropea et al. ...
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bioRxiv - Bioengineering 2019Quote: ... TGFB1-bio-His (proTGFβ) which was a gift from Gavin Wright (Addgene plasmid # 52185) [17] and HA-OVOL2 (OVOL2 ...
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bioRxiv - Cell Biology 2019Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol(Tropea et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
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bioRxiv - Biochemistry 2022Quote: ... pET29b-IPP1-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124137). 15µL of Lemo21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... coli MG1655 into a His-SUMO-TEV (HST) plasmid backbone (Addgene product number: 48313). Each HST construct was grown in LB to an OD600 of 0.4 at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293/T17 cells were transfected with DNAJB1-PRKACA K128H plasmid along with psPAX2 (Addgene plasmid #12260, gift from Didier Trono) and pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: Rhodopsin protein was expressed in HEK293 cells using transient transfection (pcDNA3 rod opsin construct, a gift from Robert Lucas (Addgene plasmid # 109361 ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected in 96-well plates with the 8xGTIIC-luciferase plasmid (firefly luciferase, # 34615, Addgene, Watertown, MA, US) and the pRL-SVl40P plasmid (Renilla luciferase ...
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bioRxiv - Cell Biology 2022Quote: ... subcloning from the following constructs: Flag-Axin1 purchased from Addgene (#109370), tdmRuby3 from Max Wilson into XLone-GFP purchased from Addgene (#96930 ...
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bioRxiv - Biochemistry 2019Quote: ... and FLAG-eIF4E2 was a gift from Dong-Er Zhang (Addgene plasmid # 17342 ...