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Citations for Addgene :
501 - 550 of 686 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE(79) was a gift from Matthew Banghart (Addgene plasmid # 166970 ...
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bioRxiv - Physiology 2023Quote: ... the pAS_4xG1 PylT FLAG-G1 PylRS Y125A tRNA/aminoacyl-tRNA synthetase plasmid (Addgene, Watertown, MA #154773) was used ...
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bioRxiv - Genetics 2021Quote: ... Putative enhancers were attached to a pes-10 minimal promoter::HIS-24::mCherry::let-858 3’UTR fragment amplified from POPTOP plasmid (71) (Addgene #34848) by using PCR stitching to create an enhancer reporter which was sequence verified and purified with a PureLink PCR purification kit (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... DNA encoding peptides derived from the C-terminal last 10 residues of the norovirus and cellular proteins were cloned into the pET-His-GST vector (Addgene:29655) with an N-terminal GST tag ...
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bioRxiv - Genetics 2022Quote: ... Enhancer reporters were produced by fusing via PCR stitching these constructs to a pes-10 minimal promoter::HIS-24::mCherry::let-858 3’UTR fragment amplified from the POPTOP plasmid [53] (Addgene #34848). The enhancer reporters were purified with a PureLink PCR purification kit (ThermoFisher ...
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bioRxiv - Synthetic Biology 2019Quote: A plasmid for SpCas9 expression (2x NLS and C-terminal His tag, pET-28a) was a gift from the Gao group (Addgene #98158).50 E ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAGEN-Ntn4 expression plasmid was constructed by cloning the full-length coding sequence out of mouse Ntn4-AP-His plasmid (Addgene 71980) using the primers in Table 1 ...
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bioRxiv - Biophysics 2024Quote: The expression plasmid for HIS-tagged AavLEA1 was obtained from the Addgene plasmid repository [pET15b-AavLEA1 was a gift from Claude Férec (Addgene plasmid # 53093)]52 The expression plasmid for HIS-tagged RvLEAMshort was made by inserting a truncated cDNA from pEThT-RvLEAM49 [pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033)] ...
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bioRxiv - Cancer Biology 2023Quote: ... from the expression vector pcDNA3.1/Myc-His(-)-HSulf-2 bearing human Sulf-2 cDNA (a gift from Steven Rosen, Addgene plasmid # 13004) (Morimoto-Tomita et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... pYM-N2339 and pFA6a-hphMX-(3×FLAG)-TEV-ProtA (gift from Michael Nick Boddy, Addgene plasmid # 52692).
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bioRxiv - Microbiology 2019Quote: ... ADRB1 was amplified from pcDNA3 Flag beta-1-adrenergic-receptor (gift from Robert Lefkowitz; Addgene plasmid # 14698). All fragments contained ~20bp overhangs and were assembled into EcoRV cut pLenti CMV Puro DEST (w118-1 ...
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bioRxiv - Cancer Biology 2020Quote: The HA-FLAG-hKDM5A.dn3 (#1048) was a gift from William Kaelin (Addgene plasmid # 14799; http://n2t.net/addgene:14799; RRID:Addgene_14799) (72) ...
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bioRxiv - Neuroscience 2020Quote: ... pRK5-EGFP-tau (plasmid # 46904) and pWZL Neo Myr Flag TBK1 (plasmid # 20648) were purchased from Addgene. The TBK1 Open Reading Frame (ORF ...
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bioRxiv - Cancer Biology 2020Quote: ... AR was cloned from pCMV-FLAG-hAR(Bai et al., 2005) (a gift from Elizabeth Wilson; Addgene plasmid #89080 ...
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bioRxiv - Cell Biology 2021Quote: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre was a gift from Robert Singer (Addgene plasmid # 84561) and was used to generate the actin isoform SINAPS reporters (Wu et al. ...
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bioRxiv - Developmental Biology 2022Quote: Sufu KO #2 was transfected with 2 μg of either 1436 pcDNA3 Flag HA (Addgene plasmid: 10792), a pcDNA Flag HA vector with the Sufu open reading frame cloned from pcDNA Su(fu ...
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bioRxiv - Cell Biology 2020Quote: ... The amplicons were inserted into pcDNA3 Flag HA plasmid (kind gift from William Sellers, Addgene plasmid # 10792) using EcoRI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... pCS2-FLAG was a gift from Peter Klein (Addgene plasmid # 16331; http://n2t.net/addgene:16331; RRID:Addgene 16331).
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bioRxiv - Developmental Biology 2019Quote: ... pCIG-CA-mTOR was generated by modifying the pcDNA3-FLAG-mTOR-S2215Y vector purchased from Addgene (# 69013), which was deposited by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... USP36 was amplified from Flag-HA-USP36 (kind gift from Wade Harper, Addgene plasmid #22579, RRID: Addgene_22579) and cloned using the BamHI and EcoRV restriction sites of a pcDNA5/FRT/TO/nEGFP vector ...
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bioRxiv - Cell Biology 2021Quote: ... USP36 was amplified from Flag-HA-USP36 (kind gift from Wade Harper, Addgene plasmid #22579, RRID: Addgene_22579) and cloned using the BamHI and EcoRV restriction sites of a pcDNA5/FRT/TO/nEGFP vector ...
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bioRxiv - Immunology 2021Quote: Plasmid pCMV14-3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian (Addgene plasmid # 145780)68 ...
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bioRxiv - Cell Biology 2022Quote: ... pLJM1-FLAG-GFP-OSBP was a gift from Roberto Zoncu (Addgene plasmid # 134659 (Lim et al, 2019)) ...
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bioRxiv - Immunology 2022Quote: ... The original plasmid pST1374-NLS-flag-linker-Cas9 (kindly donated by Xingxu Huang [30] #44758, Addgene, USA), was modified to remove the flag linker ...
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bioRxiv - Neuroscience 2022Quote: ... Expression plasmids for Pak1 (pCMV6M-Pak1) and Pak4 (pWZL-Neo-Myr-Flag-Pak4) were obtained from Addgene (plasmid #12209 and # 20460 ...
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bioRxiv - Biochemistry 2023Quote: ... Full length PER1 and PER2 were cloned into the pEZYflag vector with a flag tag (Addgene #18700) resulting in the expression clones PER2 WT_pEZYflag ...
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bioRxiv - Cell Biology 2023Quote: ... the AGO2 CDS was PCR-amplified using pFRT/FLAG/HA-DEST EIF2C2 plasmid (Addgene: Cat. # 19888[57]) as template and Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting chimera genes for the light chain and the His-tagged heavy chain of the Fabs were separately cloned into the pCAGEN vector (a gift from Connie Cepko (Addgene plasmid #11160)) 34 ...
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bioRxiv - Biochemistry 2020Quote: ... coli RP hk339-GFP (monomeric His-tagged Kif5B kinesin motor domain) expression plasmid was a gift from Ron Vale (Addgene plasmid #24431).
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bioRxiv - Biochemistry 2021Quote: ... Nsp14 was subcloned into a modified pBIG1a vector containing a pLIB-derived polyhedrin expression cassette to either contain an N-terminal 3xFlag-6His tag (sequence: MDYKDHDGDYKDHDIDYKDDDDKGSHHHHHHSAVLQ-nsp14) or no tag to generate SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164). Baculoviruses were generated and amplified in Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 2.3 x 1013 vg/mL) or 200 nL/side AAV5-CMV-HI-eGFP-Cre-WPRE-SV4 (Addgene; ≥ 1 x 1013 vg/mL) was injected at a rate of 100 nL/min into the insula of Pdynlox/lox and Oprk1lox/lox mice ...
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bioRxiv - Biophysics 2023Quote: ... was cloned into the pEG-BacMam expression vector with the GFP and 8X-His tags at the C-terminus (Addgene: Table S2). All constructs with large domain insertions and deletions were made using standard protocols for Gibson assembly (New England Biolabs ...
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bioRxiv - Biophysics 2023Quote: ... was expressed in E.coli using a gene with an N-terminal 6×His-tag and an upstream TEV-protease site cloned into pET28a(+) (Addgene plasmid #20061). MSP1D1 was purified using IMAC with further cleavage of 6×His-tag by TEV protease 50,51 ...
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bioRxiv - Molecular Biology 2024Quote: The HTLV-1 CA (Gag amino acids 132-349) was cloned into the 6×His SUMO bacterial expression plasmid (pHYRSF53, Addgene, Watertown, MA), with mutants created by using the Gibson assembly procedure ...
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bioRxiv - Molecular Biology 2024Quote: The human MPST expression plasmid with the N-terminal His tag with TEV cleavage site (MPSTA) was a gift from Nicola Burgess-Brown (Addgene plasmid #42482). This construct was co-expressed with chaperones groEL ...
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bioRxiv - Molecular Biology 2024Quote: The human MPST expression plasmid with the N-terminal His tag with TEV cleavage site (MPSTA) was a gift from Nicola Burgess-Brown (Addgene plasmid #42482). The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340) ...
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bioRxiv - Cell Biology 2020Quote: ... a fragment containing the 3×Flag-nls-cas9-nls coding sequence isolated from pBS-Hsp70-cas9 (Addgene, #46294) with same enzymes was inserted ...
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bioRxiv - Molecular Biology 2020Quote: ... with FLAG-APEX2-HA subcloned from pcDNA3-APEX2-NES (a gift from Dr. Alice Ting, Addgene plasmid # 49386) using the following primers ...
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bioRxiv - Cell Biology 2022Quote: pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A was a gift from Nicolas Manel (Addgene plasmid # 86674; http://n2t.net/addgene:86674; RRID:Addgene_86674), and Lem2 were subcloned into mScarlet-1 and mEOS-C1 ...
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bioRxiv - Biochemistry 2019Quote: ... and FLAG-eIF4E2 was a gift from Dong-Er Zhang (Addgene plasmid # 17342; http://n2t.net/addgene:17342; RRID:Addgene_17342). Control vectors were of the same backbone and tag without a gene insert ...
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bioRxiv - Microbiology 2021Quote: ... Flag-tagged full-length Human gamma-catenin construct in the pcDNA3 vector was obtained from Addgene (plasmid #16827).
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bioRxiv - Cancer Biology 2020Quote: ... AR was cloned from pCMV-FLAG-hAR(Bai et al., 2005) (a gift from Elizabeth Wilson; Addgene plasmid #89080; http://net.net/addgene89080; RRID:Addgene_89080). See Table 1 for cloning primers ...
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bioRxiv - Genomics 2020Quote: ... The pCMV-FLAG LAP2 plasmid was a gift from Joan Massague (Addgene plasmid #15738; http://n2t.net/addgene:15738; RRID:Addgene_15738) (Gomis et al. ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-mNep2-FLAG (mNeptune2-N1, a gift from Michael Davidson (Addgene plasmid # 54837; http://n2t.net/addgene:54837; RRID:Addgene_54837), Mitochondrial target sequence with linker in N-terminal ...
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bioRxiv - Cancer Biology 2021Quote: ... or RUNX1ΔEx6 were ligated into XhoI/EcoRI restriction sites in the c-Flag pcDNA3vector (Addgene plasmid #20011; http://n2t.net/addgene:20011; RRID:Addgene_20011) (Sanjabi et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid expressing FLAG-tagged wild-type human HDAC1 was a gift from Eric Verdin (Addgene Plasmid # 13820). HCT116 cells were transfected in 10 cm plates with 8 μg plasmid and 40 μl PEI ...
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bioRxiv - Developmental Biology 2022Quote: The rat Ocm gene (sequence in Figure S01) was cloned into pcDNA3.1CFP and pEGFP-N1 FLAG (Addgene #60360). Then ...
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bioRxiv - Immunology 2022Quote: ... pCAGGS-SARS2-S-FKO (C-flag) was a gift from Hyeryun Choe & Michael Farzan (Addgene plasmid # 159364; http://n2t.net/addgene:159364; RRID:Addgene_159364). After 48 hours ...
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bioRxiv - Genetics 2022Quote: ... The plenti-CAG-gate-FLAG-IRES-GFP vector was a gift from William Kaelin (Addgene plasmid #107398; http://n2t.net/addgene:107398; RRID:Addgene_107398) (82) ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lines overexpressing SLC7A11 were generated using pLenti-CMV-SLC7A11-sh926R-FLAG-IRES-Hygro (a gift from William Kaelin (Addgene plasmid #118702; http://n2t.net/addgene:118702; RRID:Addgene_118702)).Unless otherwise specified ...