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Citations for Addgene :
201 - 250 of 3696 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and pLenti-CRISPRvs-puro (Plasmid 98291, Addgene; target = GCAGCACCGAAGCTTCGTTG-AGG). Lentivirus was generated in HEK293 cells and placed directly on fibroblasts ...
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bioRxiv - Cancer Biology 2024Quote: ... the autophagosome reporter plasmid DEST-CMV mCherry-GFP-LC3B WT (Addgene, 123230) and the autophagic flux reporter plasmid pMRX-IP-GFP-LC3-RFP-LC3ΔG (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... we utilized the pLenti-CMV-TetR-blastR construct (Addgene cat. 17492). Both pLenti-CMV/TO-AXL-puroR and pLenti-CMV-TetR-blastR constructs were independently packaged into lentiviral particles ...
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bioRxiv - Cancer Biology 2024Quote: ... and subcloned into pLenti-CMV-EGFP-Blasticidin lentiviral vector (Addgene, 17445) after removal of eGFP region ...
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bioRxiv - Biophysics 2020Quote: We amplified OsTIR1 from pMK232 100 via PCR with the primers Cloning_OsTIR1_fw and Cloning_OsTIR1_rev and inserted it into the pLenti backbone from pLenti-CMV-rtTA3 (Addgene plasmid #26429) by digestion with BstXI ...
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bioRxiv - Cancer Biology 2022Quote: ... Visualization of nuclei was achieved using pLenti PGK DEST-H2B-iRFP670 (Addgene plasmid #90237).
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bioRxiv - Cancer Biology 2024Quote: ... The mutant cDNAs were transferred by Gateway reaction into pLENTI PGK Neo DEST (Addgene #19067 ...
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bioRxiv - Biochemistry 2024Quote: ... pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro (132773, Addgene), using JetOptimus transfection reagent (117-01 ...
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bioRxiv - Genetics 2020Quote: sgRNAs (Supplementary Table S1) were cloned into pLenti-Guide-Puro (Addgene) and delivered to hTERTimmortalized RPE-1 cells carrying a tetracycline-inducible promoter by lentiviral transduction ...
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bioRxiv - Molecular Biology 2020Quote: ... A lentiviral backbone was obtained from Addgene (pLenti-puro, Addgene #39481) and the CMV promoter was removed to prevent aberrant transcription by digesting the plasmid with ClaI-HF and BamHI-HF (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... The pLenti CMV rtTA3 Blast (w756-1) was a gift from Eric Campeau (Addgene plasmid #26429).
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bioRxiv - Cancer Biology 2021Quote: ... TetR was expressed in cells using pLenti CMV TetR Blast (Addgene #17492). Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria) ...
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bioRxiv - Cancer Biology 2023Quote: ... + pLenti-CMV-GFP-Neo (657-2) GFP expression vector (Addgene, Plasmid 17447). All cells were cultured with RPMI 1640 media ...
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bioRxiv - Cell Biology 2023Quote: ... Pitx2-GFP expressing cells were cloned into pLenti PGK Neo DEST (Addgene plasmid # 19067 ref68), EGFP was cloned at the C terminus of the coding sequence of Pitx2 isoform 1 (Origene ...
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bioRxiv - Microbiology 2021Quote: Transduction retrovirus particles were collected from HEK293T cells following Lipofectamine 3000 transfection of expression plasmids (pLenti CMV TetR BLAST and plenti X1 shRNA plasmids) and packaging plasmids pMD2.G (Addgene; number 12259 ...
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bioRxiv - Microbiology 2024Quote: ... were cloned into CMV Blast DEST (706–1) (a gift from Eric Campeau & Paul Kaufman, Addgene plasmid #17451) expression vector resulting in plasmid AX581 ...
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bioRxiv - Neuroscience 2021Quote: ... the successful clone was transduced with lentivirus using pLenti CMV V5-LUC Blast (Addgene, cat# w567-1), followed by blasticidin selection ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti CMV GFP Blast (659–1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17445). pHAGE-PIK3CA-H1047L was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116499 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti-CMV-GFP-Blast (659-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17445). pRL-SV40P was a gift from Ron Prywes (Addgene plasmid # 27163) ...
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bioRxiv - Cell Biology 2022Quote: ... was constructed by recombining pENTR1a emGFP-Slc37a2 iso 2 with the pLenti PGK Hygro DEST (w530-1) vector (a gift from Eric Campeau and Paul Kaufman, Addgene plasmid # 19066 ...
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bioRxiv - Cell Biology 2020Quote: Human SAR1 was subcloned into pLenti-puro (Addgene Cambridge, MA; Plasmid #39481). The plasmid containing SAR1 was transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847) and an NheI/PmeI-digested piggyBac dox inducible expression vector PB-TRE-Cas91 including a puromycin selection marker ...
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bioRxiv - Molecular Biology 2023Quote: ... was generated by cloning HSV-TK cDNA into pLenti-puro (Addgene # 312043). Lentiviral particles were generated by transfection of HEK293FT cells with either LV-mCherry ...
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bioRxiv - Cell Biology 2023Quote: ... The new vector was recombined with pLenti PGK puro (Addgene Plasmid #19068). The plasmid pLenti-PGK-GFP was kindly gifted by Ana María Cuervo (Albert Einstein College of Medicine ...
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bioRxiv - Cell Biology 2020Quote: ... cells were initially infected with pLenti-CMV-TetR-Blast lentiviral vector (Addgene, 17492) and selected using Blasticidin (10 μg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... into the pLenti CMV Hygro destination vector (gift from Eric Campeau, Addgene #19066). All gblocks were purchased from IDT.
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bioRxiv - Cancer Biology 2024Quote: ... the Sox11 coding sequence was amplified from pLenti-CMV-GFP-Sox11 (AddGene, 120387). P2A-EGFP was amplified from a self-cloned sLP-CreERT2-P2A-EGFP plasmid ...
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bioRxiv - Genomics 2024Quote: GSCs were first infected with lentiviruses harboring pLenti CMV rtTA3 Blast (Addgene, 26429) and underwent blasticidin selection for 5 days ...
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bioRxiv - Microbiology 2020Quote: ... PspCas13b on pENTR1A was shuttled to pLenti-CMVtight-Blast-DEST(Campeau et al., 2009) (Addgene #26434) with Gateway cloning (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: The FoxO1-mRuby2 construct was cloned into the pLenti-puro lentiviral backbone using Gibson cloning and the pLenti-FoxO1-Clover70 and ERKKTR-mRuby constructs23 (Addgene plasmids nb 67759 & 90231 ...
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bioRxiv - Cancer Biology 2020Quote: Human Tks5α was cloned into pCDH-CMV-MCS-EF1-Puro (Addgene) with a GFP sequence fused at the C-terminus ...
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bioRxiv - Cancer Biology 2023Quote: ... were amplified by PCR and cloned into pLenti-GFP-puro (Addgene plasmids #17481) Lentivirus were produced by transforming pLenti constructs ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids pLenti-Cas9-puro and psPAX2 and pMD2.G were purchased from Addgene. The sgRNAs specific for mouse Sik1 ...
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bioRxiv - Cancer Biology 2024Quote: ... We generated two customized sgRNA construct backbones: pLenti-sgStuffer-GFP-Puro (Addgene 208349) and pLenti-sgStuffer-mCherry-Puro (Addgene 208350) ...
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bioRxiv - Molecular Biology 2021Quote: ... pLenti-CMV-GFP-Hygro was a gift from Eric Campeau & Paul Kaufman (Addgene #17446), psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmid # 12260 and # 12259) ...
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bioRxiv - Systems Biology 2021Quote: ... Lyn-FRB was cloned into the pLenti CMV blast destination vector (Addgene plasmid #17451). To generate lentivirus ...
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bioRxiv - Cell Biology 2021Quote: ... pLenti-CMV-Blast-PIP-FUCCI was a gift from Jean Cook (Addgene plasmid # 138715) (Grant et al ...
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bioRxiv - Cell Biology 2021Quote: ... promoter regions were amplified and cloned into pLenti CMV GFP Blast vector (AddGene, #17445) (Campeau et al. ...
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bioRxiv - Cell Biology 2022Quote: RPE-1.iPLK4 and RPE-1.iPLK41-608 cell lines were generated using pLenti-CMV-TetR-Blast lentiviral vector (Addgene, 17492) and selected using Blasticidin (10 µg/mL) ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-T/t antigens expressing lentiviruses using vectors pLenti CMV-RasV12-Neo (w108-1) (HRAS G12V, #22259, Addgene), and pLenti-CMV/TO-SV40 small + Large T (w612-1 ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... For assessing transduction efficiency a CMV-GFP-Puro-expressing lentivirus (Addgene #17448) was used at MOI=1 ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... OsTIR1 and AtAFB2 plasmids were used (AtAFB2) (pMK232 CMV-OsTIR1-PURO, Addgene #72834 ...
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bioRxiv - Cancer Biology 2021Quote: ... LentiCRISPRv2-puro (#98290) and pLenti-PGK-Hygro-KRAS(G12V) (#35635) were obtained from Addgene.
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bioRxiv - Molecular Biology 2023Quote: This construct was then cloned into the pLenti-Puro-AU-flip-3xBsmBI (Addgene #196709) (75 ...
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bioRxiv - Neuroscience 2022Quote: ... the pLenti- TetO-hNGN2-puro (pLV-TetO-hNGN2-Neo deposited by Kristen Brennand, Addgene plasmid # 99378 ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized constructs with a Kozak sequence were subcloned into pLENTI-puro (Addgene #39481) to create MCPyV ST ko/co ...
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bioRxiv - Molecular Biology 2022Quote: ... WM1366 was transduced with pLenti-CMV-rtTA3-Blast (a gift from Eric Campeau, Addgene #26429) and selected with 20 μg/mL blasticidin (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... blasticidin (Sigma-Aldrich, 5 μg/mL for four days; pLenti CMV Blast – Addgene plasmid #17451), or hygromycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: RNH1-KO THP1 cells were infected with lentiviruses expressing RNH1 (pLenti CMV Blast GFP-RNH1) or the empty vector pLentiCMV-GFP-Blast (659-1, Addgene) as previously described (Papin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS 2-6-3 were transduced with pLenti CMV rtTA3 Blast (w756-1, plasmid #26429; Addgene, gift from E. Campeau) for the expression of rtTA ...