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Citations for Addgene :
201 - 250 of 1063 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: After co-transfection for 36 hours with plasmid DNA encoding the relevant SNAP-tagged receptor (1 μg) and AKAR4-NES (1 μg; a gift from Dr Jin Zhang, Addgene plasmid #647270), wild-type or dual β-arrestin knockout HEK293 cells were suspended in HBSS in black 96 well plates ...
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bioRxiv - Neuroscience 2024Quote: ... 0.85 μl of virus coding for either muscarinic-receptor-based DREADD AAV8-hSyn-hM4Di(Gi)-mCherry or control virus AAV8-hSyn-EYFP (AddGene, Cambridge, MA) was injected bilaterally into either the mPFC (AP ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs: 12251, 12253, 12259) (67) ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The corresponding primers were cloned into the sgRNA expression cassette of pGREB31 (Addgene). The pTGE2 vector was constructed as described above based on target site sequence 12 (Figure 1B ...
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bioRxiv - Bioengineering 2024Quote: ... pFA6a-link-yoEGFP-SpHis5 (primers OM-8 and OM-9) (Addgene plasmid #44836), and pBTK522::FAST-PETase 21 (gift from Hal Alper ...
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bioRxiv - Biophysics 2024Quote: ... two primer sets were made for cpHaloTag in pCAG-HASAP (AddGene plasmid #138325) and rTRPV1 ...
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bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
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bioRxiv - Genetics 2024Quote: ... and a virus coding for the Cre-dependent expression of an excitatory DREADD receptor (AAV8-hSyn-DIO-hM3Dq-mCherry; Addgene catalog # 44361-AAV8) was injected into the LC (Fig ...
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bioRxiv - Genetics 2024Quote: ... and either a virus coding for the Cre-dependent expression of an excitatory DREADD receptor (AAV8-hSyn-DIO-hM3Dq-mCherry; experimental group) or a control virus (AAV8-hSyn-DIO-mCherry; control group; Addgene catalog # 114472-AAV8) was injected into the LC (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... 6 × 107 RPE1 cells were infected with the human sgRNA library (Human GeCKO v2 Library Cat#1000000048, Addgene) at an MOI of ∼0.3 ...
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bioRxiv - Systems Biology 2021Quote: ... human knockout CRISPR v1 library (Addgene #67989) (39) ...
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bioRxiv - Microbiology 2021Quote: The human CRISPR Brunello library (Addgene 73178) (16 ...
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bioRxiv - Neuroscience 2022Quote: ... including the human IgG1 Fc (Addgene #145165), and mouse IgG1 Fc (Addgene #28216 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human Sin1.1 was PCR-amplified from Addgene 73388 plasmid (gift from Taekjip Ha32 ...
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bioRxiv - Biochemistry 2024Quote: ... and recombinant human GST-CK2α (Addgene #27083) enzymes were produced in-house as previously described [30] ...
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bioRxiv - Cell Biology 2023Quote: ... from human pcDNA3.1-2xFLAG-SREBP1a (#26801, Addgene), pcDNA3.1-2xFLAG-SREBP1c (#26802 ...
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bioRxiv - Evolutionary Biology 2024Quote: Coding sequences for human RIPK1 (Addgene #78834), human RIPK2 (ORFeome ID #4886) ...
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bioRxiv - Biophysics 2023Quote: The full-length α-synuclein monomer (1−140) expression plasmid (pET21a-alpha-synuclein) was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 51486). The α-synuclein monomer was expressed and purified following the previous method20,21 ...
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bioRxiv - Biochemistry 2024Quote: ... The RGS domain of human RGS16 (residue 86–205) and human Gαi1 (residues 31-354) were expressed in the pNIC-SGC1 vector (Addgene), and pProEXHTb vector (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: An adeno-associated viral vector carrying the inhibitory hM4Di designer receptor exclusively activated by designer drugs (DREADDs; Armbruster et al., 2007; Rogan and Roth, 2011) was obtained from Addgene (AAV8-CaMKIIa-hM4D(Gi)-mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... gRNA primers were individually ligated into a variant of the pX335 vector (Addgene #42335) additionally containing the reporter protein GFP and a puromycin selection cassette (Cong et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10xUAS promoter fragment amplified with primers 1010.C3 and 1010.C4 from Addgene plasmid 78897 ...
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bioRxiv - Cell Biology 2021Quote: LAMP1-GFP was amplified using PCR (using primers listed in Table S2) from Addgene plasmid #34831 and then cloned into lentiviral vector FUGW (Addgene plasmid #14883 ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The synthesized oligo DNA primers for the guides were inserted into pX330 (Addgene #42230). For Tet-inducible expression of exogenous TopoII ...
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bioRxiv - Molecular Biology 2022Quote: ... was amplified using primers casI.for and casv.rev2 and introduced into SwaI-opened pBig1b (Addgene) by Gibson assembly ...
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bioRxiv - Bioengineering 2023Quote: Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
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bioRxiv - Cell Biology 2022Quote: ... having N of human coronavirus OC43 and pGBW-m4134901 (plasmid number 151922) having N of human coronavirus HKU1 229E were obtained from Addgene. Those N expression vectors were subcloned into pcDNA3.1 with C-terminal flag or EGFP tag ...
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bioRxiv - Cancer Biology 2022Quote: ... encoding human KDM6A with HA tag and pCS2-UTX-F-MT2 (40619) encoding enzyme-dead human KDM6A were purchased from Addgene. The HR and NHEJ reporter plasmids were kind gifts from Tomasz Skorski (61) ...
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bioRxiv - Cancer Biology 2021Quote: The human MYC cDNA was purchased from Addgene (pDONR223_MYC_WT ...
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bioRxiv - Cancer Biology 2020Quote: ... Human ETV1 (Addgene, Cambridge, MA, USA; plasmid #82209) and ETV5 (Horizon Discovery ...
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bioRxiv - Biochemistry 2020Quote: Plasmids encoding human full-length WDR5 (2GNQ, Addgene) or a 20aa N-terminal truncation (ΔN20 ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: Human GeCKOv2 CRISPR knockout pooled library (Addgene # 1000000049) and lenti-Cas9 plasmid were obtained from addgene (Addgene # 52962) ...
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bioRxiv - Neuroscience 2021Quote: Heterologous expression of human NaV1.2 WT (Addgene #162279)(DeKeyser et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... human TERT (LOX-TERT-iresTK; Addgene plasmid #12245), generously provided by Didier Trono ...
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bioRxiv - Biochemistry 2022Quote: ... Human BIN1-EGFP (isoform 8) (Addgene plasmid #27305) was cloned in pET15b with N-terminal 6xHis and C-terminal StrepII tags ...
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bioRxiv - Microbiology 2023Quote: The human SAM CRISPRa sgRNA library (Addgene #1000000078) was cloned into the pHW-TRPPC-NS rescue plasmid backbone for PR8 (Fig S2A ...
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bioRxiv - Biophysics 2023Quote: Human MeCP2 in the pTXB1 plasmid (Addgene #48091) was propagated in E ...
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bioRxiv - Biochemistry 2023Quote: Human BIN1-EGFP (isoform 8) (Addgene plasmid #27305) and human dynamin2 C-terminally tagged to mCherry were cloned in pET15b with an N-terminal 6xHis and a C-terminal StrepII tag ...
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bioRxiv - Neuroscience 2022Quote: ... Human VAC14 was obtained from Addgene (Plasmid #47418) and subcloned into the mCherry- C1 vector ...
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bioRxiv - Immunology 2023Quote: A lentiviral construct containing human ACE2 (Addgene 155295) or mScarlet (Addgene 85044 ...
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bioRxiv - Cancer Biology 2023Quote: ... we used human decipher module 1 library (RRID:Addgene_28289)(Diehl et al ...
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bioRxiv - Physiology 2023Quote: ... containing human Fis1 gene were purchased from Addgene. The working viral vectors were constructed from these two plasmids by Custom DNA Constructs ...
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bioRxiv - Neuroscience 2024Quote: Flag-Human CXCR7(ACKR3)-Tango (Plasmid #66265, Addgene) immune-purification was performed as described in 87 ...
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bioRxiv - Neuroscience 2024Quote: Human RBFOX1 cDNA (from pENTR-A2BP1, Addgene, 16176) or human U2AF2 cDNA (from pCDNA3-U2AF65-DYK ...
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bioRxiv - Cancer Biology 2024Quote: Human pooled metabolic CRISPR KO library (Addgene #110066), pLV-mCherry-hCdt1(1-100)ΔCy (Addgene #193759) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were co-transfected with plasmids encoding wide-type myc-human TREM2 and GFP-human TDP-43 (residues 216-414, Addgene, 28197) or GFP control by the calcium phosphate precipitation method ...
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bioRxiv - Genetics 2020Quote: sgRNA sequences targeting human USP15 were selected from the Human Brunello CRISPR knockout pooled library (Doench et al., 2016)(Addgene #73178) and further selected on the basis of high quality score in two additional online tools ...
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bioRxiv - Cancer Biology 2021Quote: ... we amplified human KEAP1 and LKB1 off of human cDNA and used Gibson assembly to replace GFP in pMCB306 (Addgene #89360) with these sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The TRPML1-GCamP6s expression construct was created by subcloning the human TRPML1 insert from the human TRPML1-EYFP plasmid 63 into the pGP backbone harboring GCaMP6s (Addgene #40753) using restriction sites BglII (N-terminal ...