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Citations for Addgene :
151 - 200 of 552 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The corresponding guide DNA sequences were cloned into the lentiCRISPRv2 plasmid (Addgene #52961) according to the instructions of the Zhang laboratory (https://www.addgene.org/52961)31 ...
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bioRxiv - Neuroscience 2021Quote: ... and donor DNA plasmid containing a neomycin-resistance cassette (adapted from Addgene, PL552). Transfected cells were selected with neomycin for one week ...
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bioRxiv - Cell Biology 2020Quote: ... The gBlock DNA was inserted into pCSCMV:tdTomato (a gift from Gerhart Ryffel, Addgene plasmid #30530 ...
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bioRxiv - Cancer Biology 2022Quote: DNA plasmids were constructed using the pLX302 and pLX304 Gateway system (Addgene, #25890). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA oligonucleotides were annealed and ligated into the lentiGuide-Cherry vector (Addgene # 170510) at the BsmBI restriction enzyme cutting sites ...
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bioRxiv - Molecular Biology 2022Quote: ... CRISPR-Prime Editing system encoding lentiviral pLenti-PE2-BSD plasmid DNAs (Addgene, # 161514) encoding CRISPR-PE containing the blasticidin resistance gene ...
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bioRxiv - Biophysics 2023Quote: DNA was synthesized from a Widom 601 sequence containing plasmid pGEMz_601 (Addgene, 26656) with primers containing a biotin and Cy3 ...
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bioRxiv - Cell Biology 2023Quote: ... The mCherry-Cry2 constructs were made by amplifying mCherry-Cry2 DNA from Addgene plasmid #101221 (gift from Brangwynne) ...
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bioRxiv - Cell Biology 2022Quote: ... The corresponding guide DNA sequences were cloned into the lentiCRISPRv2 plasmid (#52961; Addgene) according to the instructions of the Zhang laboratory (https://www.addgene.org/52961/) ...
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bioRxiv - Genetics 2023Quote: ... or an EcoRI-digested pLKO.1 backbone using T4 DNA ligase (Addgene #8453). To generate intein-split PE plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA sequence of μNS was obtained from pRS306-PHIS3-GFP-μNS (Addgene plasmid #116935 ...
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bioRxiv - Immunology 2024Quote: ... Synthesized double stranded DNA fragments were then cloned into AbVec2.0-IGHG1 (Addgene #80795), AbVc2.0-1.1-IGKC (Addgene #80796) ...
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bioRxiv - Bioengineering 2021Quote: ... The GoldenPiCS Kit was a gift from the Gasser/Mattanovich/Sauer group (Addgene kit #1000000133). All coding sequences were amplified with high-fidelity Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: TALEN plasmids were constructed using the Platinum Gate TALEN Kit (Kit #1000000043, Addgene, Cambridge, MA) as previously described (Sakuma et al. ...
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bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
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bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... a DNA fragment containing 5′-AGCCATGGGAAACATCGCAC-3′ was cloned into pL-CRISPR.EFS.tRFP (Addgene#57819) (Heckl et al. ...
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bioRxiv - Immunology 2019Quote: ... DNA sequence encoding microRNA-722 tagged with Dendra2 were amplified from (Addgene plasmid # 97163) with the following primers ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR amplified from PmCDA1-1x uracil-DNA glycosylase inhibitor protein (UGI) (Addgene #79620), evoCDA1 pBT277 (Addgene #122608) ...
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bioRxiv - Cell Biology 2021Quote: ... the cassettes were cloned via NEBuilder® HiFi DNA Assembly into AAVS1_SA_2A_Neo_CAG_RTTA3 (Addgene, #60431) (Sim et al. ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-mNep2-FLAG (mNeptune2-N1, a gift from Michael Davidson (Addgene plasmid # 54837 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-EmGFP and PGK promoter (a gift from Tyler Jacks (Addgene plasmid # 11586 ...
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bioRxiv - Developmental Biology 2022Quote: DNA constructs reported in this study are available from Addgene (plasmid ID: 176836-176841). Genetically modified cells used in this study will be available from the corresponding author with an MTA ...
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bioRxiv - Genomics 2020Quote: ... sgRNA cassettes were synthesized (Integrated DNA Technologies) and cloned into lentiGuide-Puro (Addgene, 52963) vector ...
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bioRxiv - Neuroscience 2019Quote: ... plasmid DNA mixed with either pCAG-ChR2-Venus (Petreanu et al., 2007) (Addgene #15753) or pCAG-ChETA-EYFP (1.5μg/μL) ...
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bioRxiv - Cell Biology 2019Quote: The following DNA constructs were used in this study: EGFP-PFN1 (Plasmid #56438, Addgene), Lifeact mRuby (pN1-Lifeact-mRuby ...
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bioRxiv - Biophysics 2021Quote: ... with 1 μg of DNA plasmid vector Cry2Olig-mCherry (purchased from Addgene, number 60032), and then platted on fluorodishes ...
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bioRxiv - Cell Biology 2021Quote: ... Oligo DNAs for the sgRNA were cloned into the lentiCRISPRv2 (Addgene Plasmid: no. 52961) vector or pX459 (Addgene Plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... and electroporated with the linear library DNA as well as the SS9_RNA (Addgene #71656) vector containing the guide RNA for Cas9 to target the safe harbor ...
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bioRxiv - Neuroscience 2021Quote: ... 1 million cells were nucleofected with iμg pAAV-CAG-GFP plasmid DNA (Addgene 37825) in Ingenio electroporation buffer (Mirus MIR50111 ...
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bioRxiv - Cell Biology 2019Quote: ... Annealed double strand DNAs were ligated into pSpCas9(BB)-2A-GFP (PX458) vector (Addgene) at the Bpi1 (Bbs1 ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The synthesized oligo DNA primers for the guides were inserted into pX330 (Addgene #42230). For Tet-inducible expression of exogenous TopoII ...
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bioRxiv - Neuroscience 2022Quote: ... The TauRD(P301S)-YFP plasmid DNA with virus packing pDNAs (psPAX2; pMD2.G, Addgene) was transfected into 293T cells by 1mg/mL PEI transfection reagent and incubated for 36 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 0.24μg EGFP-LC3 plasmid DNA (Addgene #11546; Watertown, MA, USA) using the FUGENE6 transfection reagent (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... DNA templates for PCR products were as follows - myo-3p from pCFJ104 (Addgene #19328), pgl-1::GFP::FLAG from DUP75 (Andralojc et al. ...
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bioRxiv - Neuroscience 2023Quote: ... either rescue construct along with two helper DNA constructs (pHR-CMV8.2 deltaR (Addgene 8454) and pCMV-VSVG (Addgene 8455) ...
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bioRxiv - Genomics 2023Quote: ... 2.5 ug of DNA (the pegRNA plasmid and the pCMV-PE2 plasmid (Addgene #132775) at a 1:2 ratio by mass of pegRNA plasmid:PE2) ...
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bioRxiv - Genetics 2023Quote: ... DNA plasmids used for transgene cloning were lenti-EF1α-dCas9-VPR-Puro (Addgene 99373) (24 ...
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bioRxiv - Cell Biology 2023Quote: ... Oligo DNAs for the sgRNA were cloned into the lentiCRISPRv2 (plasmid no. 52961; Addgene) vector or pX459 (plasmid no ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG mt-SypHer was created by PCRing the DNA encoding mt-SypHer from Addgene plasmid #48251 (a gift from Nicolas Demaurex) ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG 4xmt-iATPSnFR1.0 was created by PCR of the DNA encoding iATPSnFR1.0 from Addgene Plasmid #102556 (a gift from Baljit Khakh) ...
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bioRxiv - Plant Biology 2020Quote: ... from Sylvestre Marillonnet (Addgene kit # 1000000044). Specific parts (target gRNA or siRNA sequence ...
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bioRxiv - Synthetic Biology 2022Quote: The MoClo Toolkit (Addgene Kit #1000000044), MoClo Plant Parts Kit (Addgene Kit #1000000047) ...
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bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
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bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...
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bioRxiv - Bioengineering 2024Quote: ... including existing parts (Addgene Kit # 1000000080) and custom-made parts (Table 2) ...
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bioRxiv - Genetics 2021Quote: ... These DNA fragments were subsequently assembled into a XbaI/AscI-linearized w+attB plasmid (Addgene, Watertown ...
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bioRxiv - Genetics 2021Quote: ... is intended for small-medium sized donor DNAs (<15kb) while the p5155 (Addgene reference 175391) is a low-copy version for use with larger donor DNAs (>15kb) ...
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bioRxiv - Cancer Biology 2021Quote: ... as oligonucleotide DNA sequences and cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene; Cat#: 48138) to construct two active CRISPR vectors ...
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bioRxiv - Developmental Biology 2022Quote: E.coli strain BL21-DE3 was transformed with plasmid DNA pAG-MNase-6xHis (Addgene, plasmid #123461). Recombinant pAG-MNase was purified from cells grown in LB medium to OD600 0.6 at 37°C ...