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Citations for Addgene :
1651 - 1700 of 2007 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mEmerald-MAP4-C-1069 was a gift from Michael Davidson (Addgene plasmid # 54152; http://n2t.net/addgene:54152; RRID:Addgene_54152). MAP9 in pCR-XL-TOPO (#BC146864 ...
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bioRxiv - Microbiology 2022Quote: ... mEmerald-MAP4-C-10 (a gift from Dr. Michael Davidson (Addgene plasmid # 54152; http://n2t.net/addgene:54152; RRID:Addgene_54152)) ...
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bioRxiv - Molecular Biology 2023Quote: ... before using LR reaction to transfer the cDNA into destination vectors pLEX_305-C-dTAG or pLEX_305-N-dTAG (Addgene #91797 & #91798 ...
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bioRxiv - Cell Biology 2024Quote: ... The sequences were subsequently cloned in the destination vector MAC-tag-C, a gift from Markku Varjosalo (Liu et al., 2018) (Addgene plasmid # 108077; http://n2t.net/addgene:108077; RRID:Addgene_108077), using the Gateway™ LR Clonase™ II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... plus a five-amino-acid linker plus a C-terminal flag tag was cloned into the pENN.AAV.CB7.CI.pm20d1flag.WPRE.rBG vector (Addgene plasmid no. 132682). AAV8-GFP (pENN.AAV.CB7.CI.eGFP.WPRE.rBG) ...
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bioRxiv - Molecular Biology 2024Quote: ... pcDNA3 c-SRC (WT) was a gift from Robert Lefkowitz (Addgene plasmid # 42202 ; http://n2t.net/addgene:42202 ; RRID:Addgene_42202) (39) ...
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bioRxiv - Immunology 2024Quote: ... pCCL-c-MNDU3-X was a gift from Donald Kohn (Addgene plasmid # 81071 ; http://n2t.net/addgene:81071 ; RRID:Addgene_81071). pHAGE6 was a gift from David Baltimore.
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bioRxiv - Biophysics 2024Quote: ... and C were cloned into the pNH-TrxT vector (pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Synthetic Biology 2024Quote: An mCherry-based fluorescent DAG biosensor was made by C-terminally tagging mCherry to the C1PKCγ from Addgene plasmid #2120540 and cloning into the pCDH lentivirus backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral cloning vector pLKO.1-TRC and pcDNA3.1-C-Flag plasmids were purchased from Addgene (Watertown, MA, USA). The double-stranded oligonucleotide shRNAs targeting LINC00094 were cloned into the Age I/EcoR I sites of the pLKO.1-TRC lentiviral vector ...
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bioRxiv - Synthetic Biology 2024Quote: ... The plasmids pKanCMV-mClover3-10aa-H2B (mClover3 fused to a C-terminal histone H2B; Addgene, Plasmid#74257 (53)) ...
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bioRxiv - Biochemistry 2024Quote: ... The sequence encoding NiV L was cloned into the 438-C vector (kind gift from Scott Gradia; Addgene plasmid # 55220 ...
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bioRxiv - Neuroscience 2024Quote: ... mEmerald-EB3-C-20 was a gift from Michael Davidson (Addgene plasmid # 54076; http://n2t.net/addgene:54076; RRID:Addgene_54076)
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bioRxiv - Genetics 2020Quote: ... Ishikawa cells were plated at a density of ~300,000 cells per well in 6 well plates and transfected the following day with 1650 ng Cas9 plasmid (Addgene 62988, a gift from Feng Zhang), 550 ng of each guide RNA (Table S2) ...
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bioRxiv - Systems Biology 2022Quote: ... we prepared the vector backbone by incubating 5 ug of CROPseq-Guide-Puro (Addgene #86708) for 2 h at 37 °C with 2 ul of FastDigest Mph1103I (ThermoFisher cat ...
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bioRxiv - Cancer Biology 2020Quote: pCFDg1-5 gRNA-tRNA array was constructed stepwise as previously described using pCFD5 (Addgene #73914)11 as a template and V8 targeting gRNAs.
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 5 μg of mEmerald-Kinesin11-N-18 plasmid (Addgene number: 54137) 24 hours prior to imaging ...
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bioRxiv - Developmental Biology 2020Quote: ... into the HindIII/XbaI site 5’ of the Gateway cassette of pMpGWB401 (Addgene enry #68666). The reversely transcribed MpSUK1 locus (2.7kb ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (5×1011 gc/mL; Addgene) bilaterally into the lateral hypothalamus of the previously characterized and validated MCH-Cre mice42 ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Bioengineering 2022Quote: ... pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (RRID:Addgene_17448)[33] ...
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bioRxiv - Neuroscience 2024Quote: Intracerebroventricular injections of 5 μL of either AAV9-hSyn-DIO-hM3Dq-mCherry (Addgene # 44361-AAV9) (90 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.25 µL of EnvA G-Deleted Rabies-mCherry (diluted 1:5 in dPBS; Addgene: 32636) was injected in the basal forebrain using the same coordinates ...
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bioRxiv - Immunology 2022Quote: ... pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17448 ...
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bioRxiv - Neuroscience 2024Quote: ... while the Halo tag sequence was amplified from the TUBB-5 Halo plasmid (Addgene, #64691). These elements ...
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bioRxiv - Microbiology 2024Quote: ... pLenti PGK GFP Puro (w509-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19070 ...
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bioRxiv - Biochemistry 2024Quote: ... blasticidin (Sigma-Aldrich, 5 μg/mL for four days; pLenti CMV Blast – Addgene plasmid #17451), or hygromycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... glycans were released by treatment with 2.0 µL of PNGase F for 20 hr at 37°C (in-house, Addgene #114274 ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were digested with 2.0 µL of PNGase F for 20 h at 37°C (in-house, Addgene #114274 ...
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bioRxiv - Immunology 2021Quote: ... and a plasmid encoding the spike protein (with a C-terminal truncation) from either SARS-CoV (Addgene cat 170447), SARS-CoV-2 53 ...
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bioRxiv - Cancer Biology 2020Quote: ISG15-PA was synthesised in the Kessler lab using the ISG15 C-term (79-156) construct from Addgene (#110760) and DUB ABP assays were performed as previously described69 ...
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bioRxiv - Neuroscience 2020Quote: ... Dphox and phox vectors were C-terminally tagged with GFP by infusion cloning in frame into CAG-GFP (Addgene) using the following primers ...
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bioRxiv - Neuroscience 2022Quote: ... was digested with NheI/KpnI and ligated with similarly digested pPD96.52 (Fire lab C. elegans Vector Kit 1999; 1608: L2534, Addgene) to generate pKMC166 (myo-3p::mCherry) ...
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bioRxiv - Developmental Biology 2022Quote: ... that contain DENDRA-expressing sequences optimized for use in C. elegans (Gallo et al. 2010) are annotated in Addgene as containing DENDRA2 (e.g., pEG545, Addgene plasmid #40116 and pEG345 ...
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bioRxiv - Biochemistry 2022Quote: The C-terminal P2A-EGFP sequence was added to SaABE8e or pCMV-PE2 (Addgene IDs 138500 and 132775, respectively), and the N-terminal BPNLS was added to an SpCas9 plasmid similar to pCMV-T7-SpCas9 (Addgene plasmid ID 139987 ...
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bioRxiv - Cancer Biology 2022Quote: ... pTag-RFP-C-h-Rab11a-c-Myc was a gift from James Johnson (Addgene plasmid #79806; http://n2t.net/addgene:79806; RRID: Addgene_79806)66 ...
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bioRxiv - Biochemistry 2021Quote: ... the specific sybody and the C-terminal MBP were cloned in parallel into the expression vector pBXNPH3M (Addgene #110099) 38–40 using FX cloning 41 ...
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bioRxiv - Genetics 2020Quote: ... The guides were inserted into a deadCas9-KRAB-T2A-GFP lentiviral backbone containing both the guide RNA under the U6 promoter and dead-Cas9-KRAB and GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, a gift from Charles Gersbach, Addgene plasmid #71237 RRID:Addgene_71237). The guides were inserted into the backbone using annealed oligos and the BsmBI cloning site ...
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bioRxiv - Cell Biology 2020Quote: ... The codon-optimized mCherry sequence with synthetic introns and a C-terminal linker was amplified from pJJR83 (Addgene #75028). Correct amplification and assembly was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: Full-length and C-terminal truncated KDM6B cDNA was amplified by PCR from KDM6B plasmids (Addgene, #21212 and #21214) and subcloned into pLVX-Ubc-FLAG vector ...
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bioRxiv - Biochemistry 2020Quote: ... After cooling on ice for five min the sample was treated with 5.0 µL of PNGase F for 20 hr at 37 °C (in-house, Addgene #114274 ...
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bioRxiv - Cell Biology 2021Quote: ... Either a control vector (c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid #20011; http://n2t.net/addgene:20011; RRID:Addgene_20011(47)) ...
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bioRxiv - Cell Biology 2023Quote: ... mRuby2-Actin-C-18 was a gift from Michael Davidson (Addgene plasmid # 55889; http://n2t.net/addgene:55889; RRID: Addgene_55889). PaGFP-UtrCH was a gift from William Bement (Addgene plasmid # 26738 ...
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bioRxiv - Cell Biology 2023Quote: ... mPA-GFP-MyosinIIA-C-18 was a gift from Michael Davidson (Addgene plasmid # 57149; http://n2t.net/addgene:57149; RRID: Addgene_57149). pBa-KIF5C 559-tdTomato-FKBP was a gift from Gary Banker (Addgene plasmid # 64211 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dnmt3a CD and the C-terminal part of mouse Dnmt3L (3a3L) were amplified from pET28-Dnmt3a3L-sc27 (Addgene #71827). The resulting constructs (collectively ...
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bioRxiv - Cell Biology 2024Quote: Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid #45203; http://n2t.net/addgene:45203; RRID:Addgene 45203). Cells were transiently transfected with a and plated onto glass-bottom plates ...
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bioRxiv - Molecular Biology 2024Quote: ... The G418 (Geneticin) resistance gene was subcloned from pDEST-CMV-C-eGFP (a gift from Robin Ketteler, Addgene: 122844) and inserted into pLX303-DD-HA-ER-I-PpoI using In-Fusion cloning ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We first constructed pKB11 by replacing the TRP1 auxotrophic cassette of pDEST-DHFR F[1,2]-C (TRP1) (Addgene #177795) (Marchant et al ...
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bioRxiv - Neuroscience 2023Quote: ... The guides were inserted into a deadCas9-KRAB-T2A-GFP lentiviral backbone containing both the guide RNA under the U6 promoter and dead-Cas9-KRAB and GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, a gift from Charles Gersbach, Addgene plasmid #71237 RRID:Addgene_71237). The guides were inserted into the backbone using annealed oligos and the BsmBI cloning site ...