Labshake search
Citations for Addgene :
1451 - 1500 of 10000+ citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... using second generation packaging plasmids pMD2.G and psPAX2 (Addgene plasmid #12259 and #12260) using Lipofectamine LTX Reagent (ThermoFisher ...
-
bioRxiv - Cancer Biology 2022Quote: ... The plasmids were packaged into Lentiviral particles using the 2rd generation packaging plasmid (Addgene). 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio ...
-
bioRxiv - Molecular Biology 2022Quote: ... guide sequences were cloned into the pX330 sgRNA expression plasmid 90 (Addgene plasmid 42230), and the full target site was cloned into pM427 ...
-
bioRxiv - Cancer Biology 2022Quote: The pA-Tn5 plasmid was a generous gift from Steven Henikoff (Addgene Plasmid #124601). The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB) ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the envelope plasmid pMD2.G (Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259), both gifts from Didier Trono.
-
bioRxiv - Developmental Biology 2021Quote: ... Xenopus transgenic plasmids were constructed using the pI- SceI-d2EGFP plasmid backbone (Addgene 32674). First ...
-
bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
-
bioRxiv - Cell Biology 2022Quote: Microtubules were visualised by expressing a plasmid containing β-tubulin-mCherry (Addgene plasmid #175829).
-
bioRxiv - Molecular Biology 2022Quote: ... The pAV-U6+27-Tornado-Broccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles ...
-
bioRxiv - Biophysics 2020Quote: ... both plasmids hEGFR-EGFP and mEGFP-N1 (gift from Michael Davidson, Addgene plasmid #54767) were digested with AgeI and NotI (New England Biolabs GmbH ...
-
bioRxiv - Biophysics 2019Quote: The plasmids mEmerald-Zyxin-6 (Addgene plasmid # 54319; http://n2t.net/addgene:54319; RRID: Addgene_54319) and mCherry-Paxillin-22 (Addgene plasmid # 55114 ...
-
bioRxiv - Synthetic Biology 2019Quote: Plasmids encoding transcription templates were constructed using pUC19-T7-3WJdB-T (Addgene plasmid #87308) as a backbone ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the individual CRISPR components (Cas9 expressing plasmid or sgRNA expressing plasmid: MCB306, Addgene Plasmid #89360 or MCB320 ...
-
bioRxiv - Molecular Biology 2019Quote: ... HA-SUMO-2 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48967). Flag-PIAS4 WT plasmid was a gift from Ke Shuai (Addgene plasmid # 15208) ...
-
bioRxiv - Molecular Biology 2019Quote: ... HA-SUMO-1 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48966). HA-SUMO-2 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48967) ...
-
bioRxiv - Cancer Biology 2021Quote: ... pRK5-TGFβRI-FLAG plasmid (#14833) and pCMV5B- TGFβRII plasmid (#11766) were purchased from Addgene.
-
bioRxiv - Biochemistry 2021Quote: ... plasmid only or additionally with 125 ng of Lyn11-FRB-mCherry plasmid (Addgene, #38004) using Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... Plasmid pAc-sgRNA-Cas9 was a gift from Ji-Long Liu (Addgene plasmid # 49330). The codons of LwaCas13a gene were optimized to Drosophila preference ...
-
bioRxiv - Microbiology 2021Quote: ... RNA motif plasmid cloning backbone [(pRMB) (Addgene plasmid # 107253; http://n2t.net/addgene:107253; RRID:Addgene_107253)] ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 µg of the resulting plasmid and 0.5 µg of plasmid hCas9 (Addgene 41815) were co-transfected using 3 µl of Turbofect transfection reagent (Thermo Fisher) ...
-
bioRxiv - Genomics 2021Quote: ... and cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene plasmid #62988) as described in Ran et al ...
-
bioRxiv - Immunology 2021Quote: The plasmid pHIV-EGFP was gifted by Bryan Welm & Zena Werb (Addgene plasmid #21373) and pMD2.G and psPAX2 were gifted by Didier Trono (Addgene plasmid #12259 and #12260) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2) the NLS-Cas9 gene from the pHsp70 Cas9 plasmid (Addgene plasmid #46294 (32)) connected to the promoters and 3’-UTRs from the β2tubulin (AAEL019894) ...
-
bioRxiv - Bioengineering 2022Quote: ... were generated by cutting the same previous backbone plasmid OA-984 (Addgene plasmid #120363), with the restriction enzymes AvrII and AscI ...
-
bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151) ...
-
bioRxiv - Biochemistry 2022Quote: ... These plasmids were co-expressed with the pKW2-EFSep phosphoserine machinery plasmid (Addgene #173897). All amino acid sequences of recombinantly expressed 14-3-3 constructs can be found in the Supplementary information provided.
-
bioRxiv - Cell Biology 2022Quote: For transient cell transfections the following plasmids were used: talin-mCherry (Addgene plasmid # 55137), vinculin-mCherry14 ...
-
bioRxiv - Cell Biology 2022Quote: The following plasmids were used: H2B-mCherry in pcDNA3 (Robert Benezra; Addgene plasmid #20972), pGEMHE-eGFP-MAP4 (Jan Ellenberg ...
-
bioRxiv - Cell Biology 2022Quote: ... each TF expression plasmid was co-transfected with the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene ...
-
bioRxiv - Genomics 2022Quote: The hSTARR-seq_ORI plasmid vector was a gift from Alexander Stark (Addgene plasmid #99296) and the pcDNA3-EGFP was a gift from Doug Golenbock (Addgene plasmid #13031) ...
-
bioRxiv - Neuroscience 2022Quote: ... The donor plasmids were constructed by modifying the AAVS1-CAG-hrGFP (Addgene plasmids #52344) and replacing the GFP with Cas9 (Addgene plasmids #42230) ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
-
bioRxiv - Bioengineering 2022Quote: ... nanopore reads were aligned to plasmids carrying either gFLE (1154B, Addgene as plasmid #187238) or Cas9 (21 ...
-
bioRxiv - Cancer Biology 2024Quote: Plasmid expressing constitutively activate RhoA was ordered from Addgene (pSLIK CA RhoA, Plasmid #112894) (MacKay and Kumar ...
-
bioRxiv - Genetics 2023Quote: ... The gRNA plasmid libraries were combined with VSVg (Plasmid #8454, Addgene, Watertown, MA, USA) and psPAX2 (Plasmid #12260 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Cas9 mRNA was in vitro transcribed from pSpCas9 plasmid (PX165; Addgene plasmid #48137) using Ambion mMESSAGE mMACHINE T7 Transcription Kit ...
-
Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... pLVX-eGFP-Cre (plasmid #86805) and pLVX-GFP (plasmid #17448) were purchased from Addgene. IRSp53 I-BAR domain was amplified from the pLVX-IRSp53 plasmid and ligated into a pRK5-myc backbone using SalI and NotI.
-
bioRxiv - Cell Biology 2024Quote: ... a pME-Lifeact (65) plasmid was a gift from Rob Parton (Addgene plasmid #109545). Using gateway cloning ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 3 μg of PX459 plasmid (kind gift from Feng Zhang; Addgene plasmid ##62988) expressing both cas9 and a cloned gRNA (5’-TCTCCCATGCATTCAAACTG-3’ ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with lentivirus generated from the PIP-FUCCI plasmid (Addgene plasmid #138715) as described above ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the viral envelope plasmid pMD2.G (gift from Didier Trono; Addgene plasmid # 12259) using Lipofectamine 3000 Transfection Reagent (Invitrogen) ...
-
bioRxiv - Developmental Biology 2024Quote: Plasmid pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) (Addgene, plasmid #42335; Cong et al., 2013) was used to synthesize DNA templates containing the single guide (sg)RNA scaffold ...
-
bioRxiv - Developmental Biology 2024Quote: ... The Minos transposase (pBlueSKMimRNA) plasmid was a gift from Michalis Averof (Addgene plasmid #102535). The piggyBac transposase (pT7mRNA-PB transposase ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids pLJM1-FLAG-GFP-OSBP was from Addgene (Plasmid #134659; (Lim et al., 2019)) ...
-
bioRxiv - Biochemistry 2023Quote: ... Then PCR products were Gibson-cloned into the PEmax mRNA plasmid (Addgene plasmid #204472) digested with RsrII and XhoI enzymes ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Plasmids encoding NS3a and CP5 were obtained from Addgene (Addgene plasmid #133608 and #133614). Cloning was performed similarly to the peptide-based CAST designs through PCR amplification and Gibson Assembly of the fragments.
-
bioRxiv - Genomics 2022Quote: The lentivirus backbone plasmid was a gift from Kazuhiro Oka (Addgene plasmid #72263, RRID:Addgene_72263). This vector was digested with BspDI and KpnI and gel purified ...
-
bioRxiv - Immunology 2022Quote: ... and −8 were cloned into the LentiCRISPR v2 plasmid (Feng Zhang, Addgene plasmid #52961). The sequences for the guide RNAs were as follows ...