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Citations for Addgene :
1301 - 1350 of 10000+ citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... nmrHAS2 coding sequence was subcloned into the pCALNL-GFP plasmid (Addgene plasmid # 13770) to replace GFP ...
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bioRxiv - Cell Biology 2023Quote: ... and transiently transfected for 24h with 10μg psPax2 packaging plasmid (plasmid #12260, Addgene), 5μg pMD2.G envelope plasmid (plasmid #12259 ...
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bioRxiv - Microbiology 2023Quote: The following plasmids were used for lentivirus production: psPAX2 packaging plasmid (Addgene, #12260), pMD2-G VSV-G envelope plasmid (Addgene ...
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bioRxiv - Immunology 2023Quote: Plasmid encoding for the spycatcher003-mi3 particles was acquired from Addgene (Plasmid #159995) and transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... 2XPDZ-mCherry-Larg(DH) (Plasmid #80407) plasmids were bought from Addgene (Watertown, MA). pLVX ...
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bioRxiv - Molecular Biology 2023Quote: The pHis-hTGM2 plasmid was gifted from Byung Il Lee (Addgene plasmid #100719). The His6-tagged TGM2 C277A mutation was cloned by site-directed mutagenesis using pHis-hTGM2 as the template and the following primer sequences ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pEZ-Tet-pLKO-hygro (Addgene plasmid 85972; (Frank et al., 2017)) was adapted to express sgRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... into the mTurquoise2-N1 plasmid (Addgene plasmid #54843;http://n2t.net/addgene:54843;RRID:Addgene_54843 ...
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bioRxiv - Synthetic Biology 2023Quote: ... helper shuttle plasmid pLH01 (a kind gift from Sheng Yang -Addgene plasmid # 117261), which contains L ...
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bioRxiv - Molecular Biology 2024Quote: A pET28b plasmid encoding C-terminal 6xHis-tagged nuclear Pif1 (Addgene plasmid #65047)64 was employed to express and purify Pif1 from E ...
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bioRxiv - Neuroscience 2024Quote: ... were obtained from Addgene (Tet-O-Fuw-Ascl1 Addgene plasmid #27150, pLV.PGK.mLmx1a Addgene plasmid #33013, Nurr1 Addgene plasmid #35000 ...
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bioRxiv - Biochemistry 2023Quote: ... The pET28a plasmid carrying the MSP1E1 gene was obtained from Addgene (plasmid #20062). The membrane scaffold protein (MSP1E1 ...
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bioRxiv - Genetics 2024Quote: ... we transfected the pU6-sgRosa26-1_CBh-Cas9-T2A-BFP plasmid (Addgene Plasmid # 64216) which contains a Rosa26 targeting gRNA and the pTLR repair vector (Addgene Plasmid # 64322 ...
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bioRxiv - Cancer Biology 2021Quote: ... or the third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) were mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Cancer Biology 2021Quote: The lentivirus was packed in HEK293T cells by transfecting lentiviral expression vector plasmids together with the lentiviral packaging plasmid (psPAX2, Addgene plasmid # 12260) and the envelope protein expressing plasmid (VSV-G ...
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bioRxiv - Developmental Biology 2020Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were co-transfected with pAAV-Cre-GFP and pAAV-CMV-DIO-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA plasmids or pAAV-EF1a-DIO-mCherry plasmids (Addgene plasmid #50462) at the ratio of 1:3 using X-tremeGENE HP DNA Transfection Reagent (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... sh3_MDC1 5’-GTCTCCCAGAAGACAGTGA-3’ cloned into pSUPERblast plasmid (Stewart et al, 2003) The pSpCas9(BB)-2A-puro plasmid (a generous gift from Dr. Feng Zhang, Addgene plasmid #62988) was used for CRISPR/Cas9 mediated knockout of SMCHD1.
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bioRxiv - Genomics 2020Quote: ... The LentiSAMPHv2 plasmid was assembled by adding the P2A-MS2-p65-HSF1 fragment amplified from the lentiMPH v2 plasmid (Addgene plasmid #89308) (32 ...
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bioRxiv - Genomics 2020Quote: The pCW-Cas9-2A-EGFP plasmid was constructed by replacing the FseI-BamHI fragment in the pCW-Cas9 plasmid (Addgene plasmid #50661) (21 ...
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bioRxiv - Bioengineering 2020Quote: ... The construction of PEgRNA transcript carrying plasmid: The empty PEgRNA plasmid was modified from the pgRNA-bacteria (ColE1 ori, Addgene plasmid #44251)30 by removing the 20 bp spacer ...
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bioRxiv - Cell Biology 2021Quote: ... HEK 293 cells were transfected with pLJM1-eGFP or pLJM1-DN-FYN plasmid together with lentivirus packing and envelope plasmids pMD2.G (gift from Didier Trono, Addgene plasmid# 12259) and psPAX2 (gift from Didier Trono ...
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bioRxiv - Biochemistry 2021Quote: ... and 2000 ng total plasmid DNA per dish including the 50-1000 ng FP-tagged encoding plasmids supplemented with an empty plasmid vector (pCAG-FALSE, Addgene plasmid #89689) depending on the aimed fluorescence level [35] ...
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bioRxiv - Synthetic Biology 2022Quote: ... and pAJM.771 were gifts from Christopher Voigt34 (pAJM.717 - Addgene plasmid # 108517 // pAJM.718 - Addgene plasmid # 108519 // pAMJ.771 - Addgene plasmid # 108534). Then ...
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bioRxiv - Immunology 2020Quote: ... the lentiCas9-v2 lentivirus were produced from HEK-293FT cells transfected with the lentiCas9-v2 plasmid mixed at a 2:1:1 DNA ratio of the lentiviral packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Neuroscience 2022Quote: ... the PG13-mCherry p53 reporter plasmid was generated by replacing the luciferase sequence of PG13-Luc reporter plasmid (el-Deiry et al. 1993) (Addgene plasmid #16442), which contains 13 copies of a p53-binding site followed by the polyoma promoter ...
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bioRxiv - Synthetic Biology 2019Quote: The construction of the plasmid library is based on plasmids previously published: pJBL2801 was a gift from Julius Lucks (Addgene plasmid 71207) and was used for cloning the STARs and triggers ...
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bioRxiv - Developmental Biology 2021Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... Non-replicating lentiviruses were generated by transient co-transfection of the transfer plasmids into HEK293T together with the packaging plasmids pMDL (Addgene Plasmid #12251), pRSV-REV (Addgene Plasmid #12253 ...
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bioRxiv - Cancer Biology 2020Quote: ... The ECFC-EC and cancer cells were transduced with lentivirus expressing mCherry (LeGO-C2, plasmid # 27339), green fluorescent protein (GFP) (LeGO-V2, plasmid # 27340), or azurite (pLV-Azurite, plasmid # 36086) (Addgene, Cambridge, Massachusetts)39,40 ...
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bioRxiv - Biophysics 2021Quote: ... ΔmutL strains were co-transformed with MutL/MutL(R-E) expression plasmid and pTARA plasmid (for T7 RNA polymerase expression, a gift from Kathleen Matthews, Addgene plasmid #31491)73 ...
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bioRxiv - Genetics 2019Quote: ... 600 ng of the mutant SCN5A plasmid and 100 ng of a plasmid expressing Bxb1 recombinase (a gift from Pawel Pelczar,21 Addgene plasmid #51271). On day 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... values were converted into copy numbers by measuring against a standard curve of the AAV transfer plasmid or the Ai9 plasmid (a gift from H. Zeng Addgene plasmid # 22799). The AAV genome copy number was divided by the number of nuclei to normalize for tissue input.
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bioRxiv - Developmental Biology 2022Quote: ... the five-copy plasmid obtained above was mixed with the pPGKFLPobpA plasmid (a kind gift from Dr. Philippe Soriano – Addgene plasmid 1379357), encoding FLPo recombinase and the mix was co-electroporated into ES cell clones which had confirmed replacement of Mir483 with PGKPuroΔtk allele (Extended Data Fig ...
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bioRxiv - Bioengineering 2023Quote: ... Two plasmids were chosen for further development: a plasmid containing both an empty sgRNA scaffold as well as GFP (AddGene plasmid# 99375) with a final goal of VLP generation ...
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bioRxiv - Cell Biology 2023Quote: ... which involved the co-transfection of the plasmid containing the CDC50A target sequences and the Cas9 gene with a donor plasmid (pDonor-tBFP-NLS-Neo; Addgene plasmid #80766) referred to as Donor ...
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bioRxiv - Cancer Biology 2023Quote: ... third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) was mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus containing all mentioned plasmids was generated by transfecting HEK293T cells with the appropriate plasmid along with the psPAX2 (Addgene; Plasmid #12260) and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... AsCas12a/AsCpf1 protein was purified as described47 from an expression plasmid generated by deleting the MBP sequence from plasmid pDEST-hisMBP-AsCpf1-EC (Addgene plasmid # 79007) transformed into Rosetta(DE3)pLysS Competent Cells (Novagen) ...
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bioRxiv - Immunology 2023Quote: ... were co-transfected with the pHR plasmids and second-generation lentiviral packaging plasmids pMD2.G and psPAX2 (Addgene plasmid #12259 and #12260) using Genejuice transfection reagent (EMD Millipore ...
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bioRxiv - Molecular Biology 2024Quote: Lentivirus particles for transduction of IMR90 E6E7 hTERT cells were produced by transfection of an expression plasmid with packaging and envelope plasmids gifted from Didier Trono (Addgene plasmid #12260) and Robert A ...
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bioRxiv - Cell Biology 2020Quote: ... SKO cells were transfected with pSH-EFIRES-P-GFP(1-10)opti AAVS1 Safe Harbor integration plasmid (Addgene plasmid # 129416, [35] and pCas9-sgAAVS1-2 (Addgene plasmid # 129727 [47]), and a stable pool was selected using puromycin (1 µg/ml puromycin for 6 days ...
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bioRxiv - Cell Biology 2020Quote: ... the Mouse GeCKOv2 CRISPR knockout pooled library plasmids were co-transfected with packaging plasmids pCMV-VSV-G and pCMV-dR8.2 dvpr (Gifts from Bob Weinberg, Addgene plasmid #8485 and #8455) into HEK293T cells ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293T cells were transfected with above lentiCRISPR SHMT2 plasmid or control V2 plasmid together with helper plasmids pPAX2 and UVSVG (Addgene #8454 and #12260) using Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... the retroviral FLAG-5SA-YAP and FLAG-5SA-S94A-YAP plasmids were generated via restriction-based cloning from pQCXIH-Myc-YAP-5SA (Addgene plasmid #33093 36 and pCMV-Flag-YAP-5SA/S94A (Addgene plasmid #33103 42, respectively, and pBABE-PURO (Addgene plasmid #1764 59). The CCDEL deletion was introduced in the latter via overlapping PCR with the primers YAP-DEL-CC-HA-F and YAP-DEL-CC-HA-R as described in 31 ...
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bioRxiv - Biophysics 2021Quote: ... The pDrVSP-IRES2-EGFP plasmid was a gift from Yasushi Okamura (Addgene plasmid # 80333). Voltage-clamp recording of Kv7.2 current upon depolarization-induced Dr-VSP activation was performed as described 89 with an external solution containing 144 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... the pET30-2-GAPDH plasmids was a gift from David Sabatini (Addgene plasmid # 83910) (Pacold et al. ...
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bioRxiv - Molecular Biology 2021Quote: The CD63-pEGFP C2 plasmid was a gift from Paul Luzio (Addgene plasmid #62964)(28) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 plasmid (pSpCas9(BB)-2A-BFP (a modified version of PX458 (Addgene plasmid # 48138), in which eGFP is replaced for tagBFP) ...
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bioRxiv - Genetics 2020Quote: ... the NLS:LEXA (1-214aa) fragment from the pBPnlsLexA::GADflUw plasmid [35] (Addgene plasmid #26232) was cloned in frame in a myc-BAP170 (2-1688 aa ...