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Citations for Addgene :
101 - 150 of 1672 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The injection mix was prepared in MilliQ H2O and contained 50 ng/μL Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...
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bioRxiv - Physiology 2022Quote: ... day 6-7 organoids were mechanically disrupted and re-plated into 50 μL of matrigel/AAV mixture (0.8 μL each of Cre-dependent AAV harboring tdTomato and GCaMP6s [Addgene, #28306-AAV9 and #100842-AAV9] mixed with 50 μL of matrigel [Corning]).
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bioRxiv - Cancer Biology 2023Quote: ... TERT+ cells were transiently transfected with human CA-FOXO1 (pcDNA3 Flag-FKHR-AAA mutant; Addgene) (15) ...
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bioRxiv - Neuroscience 2020Quote: ... GABAergic projection neurons in the basal forebrain were retrogradely labeled using a unilateral injection of AAVrg-hSyn-DIO-eGFP in the OB (50 nL, Catalog #50457-AAVrg, Addgene), guided with a stereotaxic apparatus (Kopf ...
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bioRxiv - Neuroscience 2020Quote: ... a series of 6 - 10 microinjections (50 - 100 nL each, 200-300 μm depth) of AAV5.Flex.ArchT.tdTomato (Addgene, #28305-AAV5) were delivered along the posterior to anterior axis of RSC (2.0 to 4.0 mm posterior ...
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bioRxiv - Physiology 2024Quote: ... plasmid using the “Golden Gate” cloning protocol.50 gRNAs were cloned into plasmids containing either puromycin resistance (gift from Feng Zhang, Addgene plasmid #52961 ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: Cells were seeded in 12-well plates and transfected at 50-70% confluence with 0.5 µg/well TOPflash (Addgene, #12456) and 0.3 µg pRL-TK (Promega ...
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bioRxiv - Biochemistry 2024Quote: The dCas9-KRAB-MeCP2 expression vector (#122205) (49) and the EXOSC4-targeting gRNA vector (pXPR, #96925) (50) were purchased from Addgene. Custom gRNA sequences (Supplementary Table 5 ...
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bioRxiv - Developmental Biology 2024Quote: ... in 180 μL TiF2 medium were mixed with 20 μL RNP (50 pmol Cas9 and 250 pmol sgRNA) or 10 μg PB-CAG-eGFP and pCAG-PBase plasmids (Addgene) in 20 μL Opti-MEM and added into 4 mm gene pulser cuvettes (total volume 200 μL) ...
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bioRxiv - Biochemistry 2020Quote: The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) system with pX330 vector (Addgene) was used to edit the CDC50A gene in HEK293S GnT1-cells as described 4 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T cells were co-transfected with the pcw107-V5 plasmid expressing CA-STAT3 (Addgene #64611), VSV-G envelope plasmid ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bruce Conklin (University of California San Francisco, CA) (Conklin et al., 1993): qo5 (Addgene plasmid #24500), qi15 (Addgene plasmid #24501) ...
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bioRxiv - Microbiology 2020Quote: ... CRISPR/Cas-9 vectors were derived from the plasmid lentiCRISPRv2 (a gift from Feng Zhang; Addgene plasmid # 52961 ...
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bioRxiv - Cell Biology 2024Quote: ... and Roger Tsien (University of California at San Diego, La Jolla, CA, USA, Addgene, plasmid # 54667) and described in (Campbell et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an ER marker (eGFP-Calnexin; Addgene: 57122), 50 ng of a Golgi marker [mTag-β-galactosidase (1-61)] ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant WNK1 kinase domain was eluted via addition of 50 nM bdSENDP1 protease (Frey et al., 2014; Addgene ID 104962) in wash buffer 4.
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bioRxiv - Cancer Biology 2020Quote: Cells were plated in 24-well plates and transfected with YAP/TAZ luciferase reporter 8XGTIIC-lux plasmid (50 ng/cm2) (Addgene 34615) together with CMV-lacZ (75 ng/cm2 ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the landing pad sequence was produced by co-transfecting 293T cells at ∼50% confluence with psVSV-G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Microbiology 2023Quote: ... and pRN-Luc (50 ng) reporter plasmids in combination with S protein expressing plasmids pTwist-SARS-CoV-2 Δ18 D614G (Addgene, 164437) or pTwist-SARS-CoV-2 Δ18 B.1.1.529 (Addgene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lentiviral particles for CEBPB knockdown were generated by transfecting shRNA plasmids (TRCN0000007440 and TRCN0000007442) or scramble shRNA control (a gift from David Sabatini; Addgene_1864 [50]), psPAX2 (a gift from Didier Trono ...
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bioRxiv - Neuroscience 2023Quote: ... mixed 1:1 with pAAV.CAMKII.Cre.SV40 (#105558; Addgene), or pAAV.CAG.GFPsm-myc.WPRE.SV40 (#98926 ...
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bioRxiv - Microbiology 2020Quote: ... the EF1a promoter driven rtTA fragment from PB-CA-rtTA Adv (gift from Andras Nagy, Addgene # 20910), and an in-frame Hygromycin selection marker connected by 2A peptide sequence ...
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bioRxiv - Molecular Biology 2023Quote: ... U6-driven sgRNA plasmids for the various Cas effectors were cloned using pBluescript sgRNA expression plasmids (Addgene #122089 ...
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bioRxiv - Microbiology 2024Quote: ... Pooled gRNAs were synthesized (Twist Biosciences, South San Francisco, CA) and cloned into lentiCRISPRv2 (Cat# 52961, Addgene), packaged into lentivirus as described above ...
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bioRxiv - Cell Biology 2024Quote: ... Of the following vectors the FP was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag ...
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bioRxiv - Developmental Biology 2024Quote: ... lentivirus containing Doxycycline-inducible plasmid construct (pCW57.1-mDux-CA, a gift from Stephen Tapscott (Addgene plasmid #99284; http://n2t.net/addgene:99284; RRID:Addgene_99284) was produced in 293T cells and used to infect an independent 2C-GFP reporter ESC line (Percharde et al ...
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bioRxiv - Neuroscience 2021Quote: ... solution carrying the jGCaMP7s gene under the human synapsin promoter (AAV1-hsyn-jGCaMP7s, ~1e12 GC/ml, 50 nl in each injection spot, Addgene plasmid #104487) was injected into the visual ...
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bioRxiv - Biochemistry 2021Quote: ... and 2000 ng total plasmid DNA per dish including the 50-1000 ng FP-tagged encoding plasmids supplemented with an empty plasmid vector (pCAG-FALSE, Addgene plasmid #89689) depending on the aimed fluorescence level [35] ...
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bioRxiv - Physiology 2022Quote: ... An optimized rat insulin promoter (RIP) (50) and the coding sequence of hM4D(Gi)-mCherry (Gi/o-coupled DREADD-mCherry fusion protein; Plasmid #75033; Addgene, Watertown, MA) (80 ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression construct containing the target sequence 5’-GGTTTGGTTGATTTCTGCAC-3’ was obtained using a PCR-fusion technique and the PCR product was injected at 15 ng/μL together with 50 ng/μL pDD162 (eft-3prom::Cas9, a gift from Bob Goldstein, Addgene plasmid #47549). After injection ...
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bioRxiv - Microbiology 2024Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988; [49] into pSBtet-pur (Addgene #60507; [50]). Cas9 was first cloned into pSBtet-pur using the following primers ...
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bioRxiv - Cell Biology 2023Quote: Donor plasmid encoding homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1 was designed by the Allen Institute for Cell Science and obtained from Addgene (AICSDP-50). The plasmid encoding NPM1-mEGFP was a gift from the Allen Institute for Cell Science (Addgene plasmid # 109122) ...
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bioRxiv - Biochemistry 2024Quote: ... a ∼1:1:1 molar ratio mixture of library transfer (lentiCRISPRv2; Addgene plasmid #52961) and packaging plasmids (psPAX2 ...
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bioRxiv - Neuroscience 2021Quote: ... DJ-1 (pGEX-5X-1-DJ1-WT; Addgene) or pcDNA plus RFP plasmid(57 ...
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bioRxiv - Neuroscience 2023Quote: ... muscarinic receptor type 3/1 (CHRM3/1, Addgene) and GFP were co-expressed at a ratio of 8:4:3:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and pRSV-Rev (at a ratio of 4:1:1:1, Addgene#12259, #12251, #12253)41 into HEK293T cells ...
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bioRxiv - Neuroscience 2020Quote: ... A small (~0.5 mm) craniotomy was made and the virus AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6s.WPRSE.SV40 (Addgene 50942, titer 1.9 × 1013 vg/ml, −50 nL) was injected into the hippocampus (from bregma ...
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bioRxiv - Cell Biology 2022Quote: ... pRK5myc RhoA L63 (RhoA CA - constitutively active) was a gift from Alan Hall (Addgene plasmid 15900; http://n2t.net/addgene:15900; RRID:Addgene_15900) (38) ...
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bioRxiv - Cell Biology 2021Quote: ... Of the following vectors the fluorescent protein was exchanged for Tq-Ca-FLITS: 3xnls-mTurquoise2 (Addgene plasmid #98817) for a nuclear tag ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) or amplified by PCR with overhangs containing BpiI (BbsI) recognition sites and assembled into pUAP1 (Addgene #63674) to create parts compatible with the phytobrick standard (3) ...
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bioRxiv - Cell Biology 2020Quote: H2B-GFP was a gift from Geoff Wahl (The Salk Institute, La Jolla, CA; Addgene plasmid no. 11680). mCherry-Nucleus-7 plasmid was generated by Michael Davidson and obtained from Addgene (Addgene plasmid no ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA for all enzymes used was ordered from Twist Bioscience (CA, USA) in the vectors pJL1 (Addgene #69496) for expression via CFPS or pETBCS ...
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bioRxiv - Neuroscience 2023Quote: ... 1:1 mixture of pENN.AAV.CamKII 0.4.Cre.SV40(AAV1; Addgene #105558 ...
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bioRxiv - Molecular Biology 2023Quote: ... and pRSFDuet-1 IntS8 AA 1-308 (Addgene #196905), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... The GFP lentiviral vector was a gift from Marius Wernig’s laboratory (Stanford School of Medicine, CA; Addgene plasmid # 30130). An empty lentiviral construct was used as control ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The sgRNA target sequences were designed with Breaking-Cas web tool62 and cloned into MLM3636 plasmid vector (Addgene #43860) through Golden Gate cloning method ...
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bioRxiv - Neuroscience 2023Quote: The virus and transgenes infused were AAV2/9-Ef1α-DIO-eGFP-htauE14 (2.05e13 vg/mL; Virovek CA, USA; the htauE14 plasmid, a kind donation from Karen Ashe, Addgene), or AAV2/9-Ef1α-Flex-eGFP (1.8E13 vg/mL ...
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bioRxiv - Developmental Biology 2024Quote: To generate Dox-inducible Dux overexpressing mouse ESCs with a 2C-GFP reporter, lentivirus containing Doxycycline-inducible plasmid construct (pCW57.1-mDux-CA, a gift from Stephen Tapscott (Addgene plasmid #99284 ...
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bioRxiv - Neuroscience 2024Quote: ... we microinjected a 1:1 ratio of AAV1.hSyn.GCaMP6s.WPRE.SV40 (Addgene) and the somatically targeted AAV1.hSyn.ChrimsonR.mRuby2.ST (University of Minnesota Viral Vector and Cloning Core ...
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bioRxiv - Cell Biology 2021Quote: ... pLKO.1 (Addgene plasmid 8453 ...