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Citations for Addgene :
1 - 50 of 1672 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and PI-Rac1 (Addgene plasmid #22028) constructs into pAAV-EF1a-double floxed-hChR2(H134R)-eYFP-WPRE (Addgene plasmid #20298)(Wu et al. ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1.syn.jGCaMP7s 50 (Addgene, 104487-AAV1 ...
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bioRxiv - Genomics 2024Quote: ... the pIS-0 vector48 (RRID: Addgene_12178, Addgene, Cambridge, MA) was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB) ...
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bioRxiv - Genomics 2024Quote: ... the pIS-0 vector48 (RRID: Addgene_12178, Addgene, Cambridge, MA) was first linearized using SacI-HF and BmtI-HF restriction enzymes (New England Biolabs (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were injected with a target transgene (50 ng μl-1) with plasmid pJL43.1 (50 ng μl-1) (Addgene) that expresses MosTase under a germline promoter and plasmid pMR910 (20 ng μl-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Xenopus transgenic plasmids were constructed using the pI- SceI-d2EGFP plasmid backbone (Addgene 32674). First ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... Control animals were injected with a control virus (pAAV-CMV-PI-eGFP-WPRE-bGH; Addgene; #105530). For both experiments ...
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bioRxiv - Neuroscience 2023Quote: ... We injected 50-100 nL of AAV (AAV2/1-Syn-jGCaMP8m-WPRE, Addgene #162375 or AAV2/1-Syn--GCaMP6s-WPRE-SV40 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... pCMV-p38-CA-EGFP and pCMV-eGFP-N1 (Addgene, 6085-1) by using standard Lipofectamine 3000 protocols (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... 50 nL pACAGW-ChR2-Venus-AAV1 (Addgene cat#20071, titer ≥ 1×10¹³ vg/mL) was released to express ChR2 throughout the cortical layers and their outgoing axons for a long-range CRACM experiment measuring reciprocal connectivity in the visual cortex (FBLM→V1 ...
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bioRxiv - Molecular Biology 2020Quote: ... digested pCDF4 (50) (Addgene plasmid # 49411 ...
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bioRxiv - Neuroscience 2023Quote: ... We injected 50-100 nL of AAV (AAV2/1-Syn-jGCaMP8m-WPRE, Addgene #162375 or AAV2/1-Syn--GCaMP6s-WPRE-SV40, Addgene #100843) either in the AC in two locations (Coordinates ...
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bioRxiv - Neuroscience 2024Quote: ... 50 nl of AAVretro-EF1a-Flpo (Addgene 55637-AAVrg, 1.10E+13, diluted 50%) was injected into right IC and 75 nl of AAV-DJ-hSyn-FLEX_FRT-mGFP-2A-synaptophysin.mRuby (Stanford Vector Core ...
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bioRxiv - Biochemistry 2020Quote: ... A 50:50 mixture by mass of pCAG-NLS-HA-Bxb1 (Addgene Plasmid #51271), for Bxb1 recombinase expression ...
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bioRxiv - Bioengineering 2022Quote: ... psPAX2 (Addgene 12260, 50 μg), and 40 μg of the transfer vector (e.g ...
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bioRxiv - Cell Biology 2022Quote: ... These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0, 50 mM NaCl, 1 mM EDTA) as recommended by Addgene (https://www.addgene.org/protocols/annealed-oligo-cloning) ...
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bioRxiv - Bioengineering 2020Quote: ... 50 ng sfGFP-N1 (Addgene #54737) was also included to assess transfection efficiency ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng pMD2.G (Addgene #12259) and 3.1 uL of FuGene HD (Promega ...
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bioRxiv - Cell Biology 2021Quote: To generate the nrfl-1(null) deletion allele a mix containing Peft-3::Cas9 (Addgene #46168; 50 ng/μl), two pairs of sgRNA plasmids targeting the 5’ or 3’ ends of the nrfl-1 open reading frame (75 ng/μl each) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 ng pBS-GGAC-ATGC (51) (Addgene plasmid # 60949 ...
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bioRxiv - Neuroscience 2023Quote: ... or EF1a-FLAG-dCas9-VPR 50 (Addgene #114195 ...
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bioRxiv - Cancer Biology 2022Quote: Retroviral pLPCX vectors expressing the mitochondrial or cytoplasmic form of Grx1-roGFP227 and roGFP2-ORP123 were obtained from Addgene (Addgene, Watertwon, CA, USA) and used to transduce FL5.12MycER cells ...
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bioRxiv - Cancer Biology 2024Quote: pLEX304mNeonGreen (Addgene; CA#162034)
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bioRxiv - Neuroscience 2022Quote: ... Three injections of AAV9-rTH-PI-Cre-SV40 (functional, titer >7×1012 vg/ml, viral prep #107788-AAV9, Addgene, US, a gift from James M. Wilson) or were made analogously to spinal injections ...
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bioRxiv - Genomics 2024Quote: ... 50 ng of Clover-PUFc plasmid (Addgene #73689), and 200 ng of the sgRNA-15xPBSc plasmid (derived from Addgene #183213 ...
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bioRxiv - Cancer Biology 2024Quote: pLBS-mScarlet (Addgene; CA#129337)
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... and FUCCI(CA) (Addgene, 153521). For EB3:tdStayGold ...
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bioRxiv - Neuroscience 2024Quote: Neurons were isolated by first virally labeling neurons from the same batch with pAAV-CAG-tdTomato for 24 hours (1:50 dilution, Addgene 59462-AAV1) prior to incorporation into miBrain or monoculture ...
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bioRxiv - Cell Biology 2020Quote: ... and pCW57.1-mDux-CA (Addgene, 99284) plasmids were used for lentivirus production.
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bioRxiv - Developmental Biology 2020Quote: ... a FLAG-tag version of the codon-optimized mouse DUX was amplified by PCR (Primers in Extended Table 1) from pCW57.1-mDUX-CA (Addgene 99284) and subcloned into the pBS31 plasmid (pBS31-FLAG_mDUX) ...
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bioRxiv - Developmental Biology 2022Quote: ... ~50 pg of in vitro transcribed Cre.zf1 mRNA (#61391, Addgene) was injected into the embryos (Horstick et al. ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid pCW57.1-mDux-CA (Addgene, 99284) was used as a template to amplify the full-length mouse Dux for in vitro transcription ...
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bioRxiv - Molecular Biology 2024Quote: The HTLV-1 CA (Gag amino acids 132-349) was cloned into the 6×His SUMO bacterial expression plasmid (pHYRSF53, Addgene, Watertown, MA), with mutants created by using the Gibson assembly procedure ...
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bioRxiv - Molecular Biology 2020Quote: ... digested pCDF4 (50) (Addgene plasmid # 49411; http://n2t.net/addgene:49411; RRID:Addgene_49411) expression vector ...
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bioRxiv - Cell Biology 2021Quote: ... or 50 ng of a mitochondrial marker (eBFP2-Mito, Addgene: 55248). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... and then pressure injected (50 nL) with AAV-Cre (AAV.hSyn.Cre.WPRE.hGH, Addgene) in the medial ACB ...
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bioRxiv - Microbiology 2020Quote: ... Cells at 50% confluence were co-transfected with pMD2G (Addgene 12259), pCMVR8.74 (Addgene 22036 ...
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bioRxiv - Developmental Biology 2022Quote: ... of pTT3-based vector backbones (50) (Addgene IDs 71471 and 36153) and shuttle vectors containing the synthesised fragments ...
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bioRxiv - Neuroscience 2023Quote: ... 50 nl of undiluted pENN.AAV.CamKII 0.4.Cre.SV40 (Plasmid #105558, AAV5, Addgene) were injected into the CA1 area of the hippocampus ...
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bioRxiv - Microbiology 2022Quote: ... and 50 mg of pEGFP-C1 Lifeact-EGFP (purchased from Addgene) with 2.5 ml of polyethylenimine max (polysciences) ...
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bioRxiv - Synthetic Biology 2024Quote: ... catalog number #12964] CA-MLCK (constitutively active mutant of human MLCK, MLCKED785-786KK) was amplified from plasmid pSLIK CA MLCK [bought from Addgene, catalog number #84647] ...
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bioRxiv - Bioengineering 2023Quote: ... together with tFucci(CA)5 plasmid29 (Addgene #153521), as per manufacture’s protocol ...
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bioRxiv - Genetics 2024Quote: ... These duplexes were then ligated into BsaI cut DR274 (Addgene 42250, (50)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Injection mixes contained eft-3p::Cas9 plasmid (50 ng/μL; Addgene #46168), single guide RNA (sgRNA ...
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bioRxiv - Microbiology 2023Quote: Plasmids expressing HA-tagged Rab9a CA or DN variants were constructed as follows: CA and DN mutant Rab9a genes were amplified from JB84 (Addgene #128908) and DsRed-rab9 DN (Addgene ...
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bioRxiv - Microbiology 2024Quote: Plasmids expressing HA-tagged Rab6a CA or DN variants were constructed as follows: CA and DN mutant Rab6a genes were amplified from EGFP-Rab6AQ72L (Addgene #49483) and EGFP-Rab6AT27N (Addgene ...
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bioRxiv - Biophysics 2022Quote: ... a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764, (50)) ...
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bioRxiv - Neuroscience 2020Quote: ... we injected 50 nl of AAVrg-hSyn1- GCaMP6s-P2A-nls-dTomato virus (Addgene) at three locations within the left auditory cortex ...
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bioRxiv - Cell Biology 2021Quote: ... the Cas9-expression plasmid pDD162 (Peft-3::Cas9, 50 ng/μL, Addgene #47549) (Dickinson et al. ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2-CAG-GFP (50 µL, 1.5 x 1013 GC/mL, Addgene 37825-AAV2) was injected intravitreally 4 mm posterior to the temporal aspect of the limbus ...