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Citations for Addgene :
51 - 100 of 1672 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... alongside 50-100 ng of a plasmid that expressed Cre recombinase (Addgene pBT140). Transfection was performed with Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... blunted and was subcloned into pAAV-CA-FLEX (Addgene, #38042) (Watabe-Uchida et al. ...
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bioRxiv - Genetics 2021Quote: CRISPR-Cas and anti-CRISPR plasmids were ordered from Addgene, thanks to gifts from many investigators (Hou et al ...
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bioRxiv - Biochemistry 2024Quote: ... Akt2 and Akt2CA: pLenti-FLAG-Akt2-CA (Addgene plasmid #64050) [19] ...
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bioRxiv - Cell Biology 2024Quote: ... The cpmTurquoise2 in pFHL-Tq-Ca-FLITS (Addgene plasmid #129628) was replaced with different cpScI or cpScA220 variants by digestion of the vector and the PCR fragments with SacI and MluI ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 ng pBS-GGAC-ATGC (51) (Addgene plasmid # 60949; http://n2t.net/addgene:60949; RRID:Addgene_60949), 1.5 μl 10x T4 ligation buffer ...
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bioRxiv - Biochemistry 2021Quote: ... HA-HIF1-alpha-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18949 [50]). pcDNA3 LATS1 was a gift from Erich Nigg (Addgene plasmid # 41156 ...
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bioRxiv - Cell Biology 2022Quote: ... pDD162 (50 ng/μl, to express Cas9; a gift from Bob Goldstein, Addgene #47549) (Dickinson et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 non-targeting control sgRNAs were randomly picked from the Brie library59 (Addgene #73633). All 500 sgRNA sequences are listed in Supplementary Information Table 1.
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bioRxiv - Molecular Biology 2020Quote: ... Purified amplicons were mixed (30 ng each) with 50 ng pJET1.2-STOP-dsRed (51) (Addgene plasmid # 60944 ...
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bioRxiv - Neuroscience 2020Quote: ... pLenti c-Jun was created by cloning human c-Jun from PMIEG3-c-Jun (a gift from Alexander Dent (Addgene plasmid #40348)[50] using BAMHI and XHOI into pLenti-puro (a gift from Ie-Ming Shih (Addgene plasmid #39481 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were co-transfected with an additional secreted BirA ligase plasmid (50) (Addgene ID 64395) included in the transfection mixture at a ratio of 10 μg DNA:22 μg PEI ...
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bioRxiv - Cell Biology 2022Quote: ... CRISPR-Cas9 was expressed from the pX330-U6-Chimeric_BB-CBh-hSpCas9 plasmid (50) (Addgene 42230) and targeted the N-terminus (first Methionine ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene, 86708). PCR cycling parameters ...
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bioRxiv - Genomics 2024Quote: ... cells were transfected with Casilio-imaging components containing 50 ng of dCas9 plasmid (Addgene #73169), 50 ng of Clover-PUFc plasmid (Addgene #73689) ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 ng reference reporter (pcDNA3.1-Nanoluc-3xFLAG-V5) and 50 ng 3xFLAG tagged constructs (Addgene #87063). Transfection was carried out using Lipofectamine 3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... using 0.1μl Tn5 stock solution (2.8mg/ml purified psfTn5-c006, Addgene plasmid #79107, in 50% Glycerol) previously loaded with standard Illumina Tn5 adapters in tagmentation buffer ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated at 50% confluency and transfected the next day with GFP-LC3 (Addgene #21073) or double transfected with GFP-LC3 and one of the FLAG-hSynj1 constructs (see below) ...
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bioRxiv - Cell Biology 2021Quote: ... The DYNLL1 overexpression cDNA clone expressed from a pcDNA3 vector obtained from Addgene (Plasmid #24265 (50)) was compared to an empty pcDNA3 control ...
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bioRxiv - Cell Biology 2022Quote: ... cell cultures were transfected at 50% confluency with mEmerald-Tomm20-C-10 plasmid DNA (Addgene, 54281), and imaged one day after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Targeting vector was generated by replacing the OsTIR1 sequence in pMGS46 plasmid (Addgene, #126580, from (50)) with AcGFP-PRE/mPRE ...
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bioRxiv - Biochemistry 2024Quote: ... entry clones were cloned into pAG415GPD-ccdB (Addgene plasmid #14146; http://n2t.net/addgene:14146; RRID:Addgene_14146, [50]) using Gateway cloning (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... The resulting amplicon and the pCW57.1 mDux-CA target vector (AddGene, #99284) (Whiddon et al. ...
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bioRxiv - Cell Biology 2022Quote: ... pSLIK CA MLCK was a gift from Sanjay Kumar (Addgene plasmid # 84647). pYFP-Paxillin was a gift from Kenneth Yamada (Addgene #50543).
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bioRxiv - Cancer Biology 2023Quote: ... tFucci(CA)5 was a gift from Atsushi Miyawaki (Addgene plasmid # 153521).
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bioRxiv - Genomics 2024Quote: ... The DUX4-inducible plasmid pCW57.1-DUX4-CA was obtained from Addgene (#99281).
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into the BbsI site of pX459 CRISPR/Cas vector (Addgene). The sequences of all sgRNAs used in this study are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified amplicons were mixed (30 ng each) with 50 ng pJET1.2-STOP-dsRed (51) (Addgene plasmid # 60944; http://n2t.net/addgene:60944; RRID:Addgene_60944), 50 ng pBS-GGAC-ATGC (51 ...
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bioRxiv - Neuroscience 2021Quote: ... single 50 nL stereotactic injections of either AA1-Syn-ChrimsonRtdTomato (Addgene #59171-AAV1; (Klapoetke et al., 2014)) or AAV5-Syn-GFP-Jaws (Addgene #65014-AAV5 ...
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bioRxiv - Developmental Biology 2022Quote: ... was used for PCR reactions with 30-50 ng of pScarlessHD-DsRed plasmid as template (Addgene #64703) or 50 ng of gBlocks or genomic DNA from the same Drosophila strain (BDSC #55821) ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Neuroscience 2024Quote: ... 50 nl of adeno-associated viruses (pAAV-hSyn-DIO-EGFP at titer 1,5 x 10*8; Addgene 50457-AAVrg or retrograde hSyn-DIO- mCherry at titer 3,75 x 10*8 ...
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bioRxiv - Molecular Biology 2021Quote: A codon adapted version of human DUX4 (pCW57.1-DUX4-CA, Addgene plasmid #99281) was cloned into an inducible ...
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-GIT1 (plasmid #15226) and pGFP-Cas (plasmid #50729) were purchased from Addgene. Full length open reading frames (ORFs ...
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bioRxiv - Developmental Biology 2021Quote: DR274 gRNA plasmids and Cas 9 protein were from Addgene (Cambridge Massachussets, USA) and New England Biolabs (Ipswich ...
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bioRxiv - Immunology 2022Quote: NFAT1 CA-RIT (IRES-GFP) retroviral vector was obtained from Addgene (plasmid # 85181). MSCV-Kcna3-Thy1.1 (pMSCV-Thy1.1:F2A:mKcna3[NM_008418.2] ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells within each well were transfected with 50 ng of the LLPS1-iLID::EGFP::FTH1 (Addgene plasmid #122149) construct containing the core FTH1 scaffolding domain attached to iLID for light-inducible interactions ...
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bioRxiv - Developmental Biology 2021Quote: ... Injection mixes were prepared in MilliQ H2O and contained 50 ng/ml Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...
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bioRxiv - Genetics 2021Quote: ... V2.0 vector pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988 (50). HEK293 WT cells were transfected with both sgRNA vectors using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... was performed at 50 °C with a mixture of Bsp119I-digested Lenti-Neo-iCas9 (Thermo FD0124; Addgene #85400), dCas9-KRAB-MeCP2-T2A amplicon ...
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bioRxiv - Developmental Biology 2021Quote: ... The gRNA expression vectors were constructed using a Cas-9 sgRNA vector (Addgene, #68463) as a backbone ...
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bioRxiv - Cell Biology 2022Quote: ... pRK5myc RhoA L63 (RhoA CA - constitutively active) was a gift from Alan Hall (Addgene plasmid 15900 ...
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bioRxiv - Cell Biology 2020Quote: ... a gift from Esteban Dell’Angelica (University of California, Los Angeles, CA; Addgene plasmid #34831). All constructs were validated by sequencing of the full ORF.
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bioRxiv - Cancer Biology 2024Quote: Plasmid expressing constitutively activate RhoA was ordered from Addgene (pSLIK CA RhoA, Plasmid #112894) (MacKay and Kumar ...
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bioRxiv - Cell Biology 2023Quote: ... The FUCCI adenoviral vector was generated by cloning tFucci(CA)5 (Addgene plasmid #153521) into the pShuttle-CMV vector ...
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bioRxiv - Neuroscience 2024Quote: ... vectors containing the promoters encoding EF1α were generated from pAAV-CA-FLEX (#38042; Addgene) by substituting the promoter ...
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bioRxiv - Cell Biology 2024Quote: ... The constitutively active form of NFAT2 was cloned from pENTR11 CA NFAT2 (Addgene #11793) into pEZY3 plasmid (Addgene #18672 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 μL of F-ABM lentiviral mix (1:1:1:1 of Addgene plasmids 27150 ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixes were prepared in MilliQ H O and contained 50 ng/ml Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...