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Citations for Addgene :
51 - 100 of 725 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... where the gpdA promoter and the trpC terminator together with the HDV self-cleaving sequence were amplified from vector pFC334 (AddGene ID # 87846)33 and LacZ alpha gene was amplified from the MoClo ToolKit vector pICH41308 (AddGene ID # 47998)55 ...
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bioRxiv - Neuroscience 2024Quote: ... we used pD454-SR mouse alpha-synuclein (Addgene plasmid, 89075; http://n2t.net/addgene:89075; RRID: Addgene_89075). Large 250 ml bacterial cultures were left overnight at 37°C with constant shaking at 600 rpm to allow for αSyn expression.
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bioRxiv - Genetics 2022Quote: ... We started with w+attB (previously deposited into AddGene, plasmid #30326), which has a fully functional white gene under control of a basal Hsp70Bb promoter (Hsp70Bb::white ...
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bioRxiv - Bioengineering 2024Quote: ... Puro2ABFP-WPRE was amplified from pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene plasmid #67974 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.5 million cells were resuspended in Mirus nucleofector solution and electroporated with 5 ug of px458 plasmid (Addgene plasmid #48138) containing a small guide RNA (see Table S1 for oligonucleotide information ...
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bioRxiv - Cell Biology 2022Quote: ... mCh-alpha-tubulin63 was a gift from Gia Voeltz (Addgene plasmid # 49149; http://n2t.net/addgene:49149; RRID:Addgene_49149). EGFP-DCX64 was a gift from Joseph Gleeson (Addgene plasmid # 32852 ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234 ...
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bioRxiv - Biophysics 2023Quote: The EGFP-alpha-synuclein gene was amplified from Addgene plasmid # 40822 (EGFP-alpha-synuclein-WT was a gift from David Rubinsztein (http://n2t.net/addgene:40822; RRID:Addgene_40822). The forward (5’-GCCCATGGTGAGCAAGGGCGAGGAG-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... was generated by Gibson assembly of the following fragments: the EF-1 alpha promoter (from Addgene #2261), the Kozak-3XFLAG fragment (from c3GIC9 ...
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bioRxiv - Developmental Biology 2021Quote: ... the lentiviral vector pHAGE hSPC-eGFP-W given from Darrell Kotton (Addgene plasmid # 36450 ...
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bioRxiv - Cell Biology 2022Quote: ... The lentiviral backbone vector pKLV2-U6gRNA5(BbsI)-ccdb-PGKpuroBFP-W (AddGene: 67974) (Tzelepis et al. ...
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bioRxiv - Microbiology 2022Quote: ... gRNAs targeting BCL2 or BCL-W were cloned in pLentiGuide (Addgene #117986) that was linearized with BsmBI (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... FU-dio PSD95-mCherry-W was a gift from Elly Nedivi (Addgene plasmid # 73919 ...
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bioRxiv - Cancer Biology 2024Quote: pLenti.CAG.H2B.Dendra2.W was a gift from Rusty Lansford (Addgene plasmid #51005; RRID:Addgene_51005). N-cad WT or W161A mutated DNA were amplified by PCR from pCAG-Ncad WT or W161A-HA (Kon et al. ...
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bioRxiv - Cancer Biology 2024Quote: pLenti.CAG.H2B.Dendra2.W was a gift from Rusty Lansford (Addgene plasmid #51005; RRID:Addgene_51005). N-cad WT or W161A mutated DNA were amplified by PCR from pCAG-Ncad WT or W161A-HA (Kon et al. ...
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bioRxiv - Genetics 2023Quote: ... pHD-DsRed-attP-w+ was a gift from Kate O’Connor-Giles (Addgene plasmid # 80898 ...
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bioRxiv - Cell Biology 2023Quote: ... pHAGE NFkB-TA-LUC-UBC-GFP-W lentiviral plasmid (Addgene plasmid # 49343) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Cancer Biology 2021Quote: ... Firefly luciferase reporters and λNHA-LacZ (a gift from W. Filipowicz; Addgene #115363) (Pillai et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... pHAGE PGK-GFP-IRES-LUC-W was a gift from Darrell Kotton (Addgene plasmid #46793 ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs were cloned into the pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W lentiviral vector (Addgene). sgRNA sequences were designed to target the following genes ...
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bioRxiv - Cell Biology 2024Quote: ... and FusionRed-SiT-15 (gift from Michael W. Davidson, Addgene; plasmid# 56133 [67]).
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bioRxiv - Biophysics 2021Quote: ... were transfected in DMEM with 2 % (v/v) FBS with 12 μg of pHR-CMV-TetO2 construct vector alongside 11 μg envelope plasmid (pMD2.G; Addgene #12259) and 11 μg packaging plasmid (psPAX2 ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Microbiology 2022Quote: ... A repair template encoding TIR1 under the control of the alpha tubulin promoter (pTUB1) and a CAT expression cassette was amplified from Addgene plasmid #87258 using oligos P1/P2 ...
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bioRxiv - Molecular Biology 2022Quote: pHAGE NFkB-TA-LUC-UBC-GFP-W was a gift from Darrell Kotton (Addgene plasmid # 49343 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5μg lentiviral guide expression vector pKLV2-U6gRNA5(BbsI)PGKpuro-2A-BFP-W (Addgene #67974) was linearised with 100 U of Bbs I-HF (NEB ...
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bioRxiv - Cancer Biology 2023Quote: PDXTs were transduced with a Cas9-expressing pKLV2-EF1a-BsdCas9-W lentiviral vector (Addgene) and selected with 10 µg/mL blasticidin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles for cas9 and HSP90-alpha were produced using HEK293T cells by con-transfection of transgene plasmid and pMD2.G (Addgene# 12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Physiology 2024Quote: ... Pparg1 or Pparg2 in NIH-3T3 cells was performed by transfecting pcDNA3.1(-) rat C/EBP alpha (#12550, Addgene, Watertown, MA, USA), pcDNA-mC/EBPb (#49198 ...
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bioRxiv - Neuroscience 2023Quote: ... mice were stereotaxically injected under isoflurane anesthesia (1% v/v in oxygen, 1 L/min) with 250 nL of AAV8-hSyn-DIO-hM3d(Gq)-mCherry (Addgene, Catalog # 44361-AAV8) at a rate of 50 nL/min using a 1000 nL Hamilton syringe bilaterally in the dorsal striatum (coordinates ...
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bioRxiv - Genetics 2021Quote: ... These DNA fragments were subsequently assembled into a XbaI/AscI-linearized w+attB plasmid (Addgene, Watertown ...
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bioRxiv - Physiology 2021Quote: ... H441 cells were transfected with Wt or D88N HSP90 (Kindly provided by W. Sessa, Addgene plasmids #22487 or #22480 ...
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bioRxiv - Physiology 2021Quote: ... RRID:CVCL_0023) were identically transfected with GFP-eNOS (provided by W. Sessa, Addgene plasmid #22444; RRID:Addgene_22444) and/or mCherry-HSP90 (provided by D ...
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bioRxiv - Cancer Biology 2024Quote: A Cas9-expressing lentivirus stock was produced by transfecting pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) into HEK293T cells together with packaging vectors (psPax2 and pMD2.G) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs: 12251, 12253, 12259) (67) ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Biophysics 2024Quote: ... and 6x-His-TEV-Snc1 were co-expressed (if crosslinked, also transformed with pEVOL-pAzF AddGene #31186, 2mM AzF and 0.2% v/v arabinose) and induced with IPTG ...
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bioRxiv - Microbiology 2020Quote: ... pMIG-w was a gift from Luk Parijs (Addgene plasmid # 12282; http://n2t.net/addgene:12282; RRID:Addgene_12282) [54] ...
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bioRxiv - Cancer Biology 2022Quote: ... by cotransfecting 112.5 µg pHAGE PGK-GFP-IRES-LUC-W (a gift from Darrell Kotton (Addgene plasmid #46793;http://n2t.net/addgene:46793 ...
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bioRxiv - Molecular Biology 2024Quote: Cell lines stably expressing Cas9 were generated by transduction using pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) lentiviral particles in the presence of 8μg/ml polybrene (Merck ...
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bioRxiv - Biophysics 2023Quote: The full-length α-synuclein monomer (1−140) expression plasmid (pET21a-alpha-synuclein) was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 51486). The α-synuclein monomer was expressed and purified following the previous method20,21 ...
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bioRxiv - Microbiology 2020Quote: ... and v) pcDNA3.1 (originally obtained from Addgene, does not contain ITRs) was used to allow the same total molar mass to be the same for each transfection group.
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bioRxiv - Molecular Biology 2023Quote: ... CAST V and inverted repeat constructs were sub-cloned from Addgene plasmids #127922 and #127924 to generate pIF1005 ...
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bioRxiv - Neuroscience 2023Quote: ... 2.5 mm V) and retrograde AAV-Syn-jGCaMP8f-WPRE virus (Addgene) was injected using a Nanoject ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/5: pAAV2/5 was a gift from Melina Fan (Addgene plasmid # 104964 ...
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bioRxiv - Cancer Biology 2022Quote: ... melanoma cells were infected with a lentivirus made using pHAGE-PGK-GFP-IRES-LUC-W (Addgene # 46793) containing coding sequences for green-fluorescent protein and firefly luciferase ...
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bioRxiv - Genetics 2023Quote: ... pHD-DsRed-attP-w+ was a gift from Kate O’Connor-Giles (Addgene plasmid # 80898; http://n2t.net/addgene:80898; RRID:Addgene_80898). We then removed the Hsp70 promoter by digesting with PmeI and XhoI ...
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bioRxiv - Biophysics 2024Quote: ... The resulting construct (pHAGE-EF1aL-nlsGFP-W) with full plasmid map and sequence are available from Addgene, plasmid #126688 ...