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Citations for Addgene :
651 - 667 of 667 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library set A and B (Addgene, 92379 and 92380) separately ...
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bioRxiv - Genetics 2022Quote: ... the NotI/XbaI PCR fragment of eggpl-CDS (GFP tag) was cloned into the NotI/XbaI sites of pJFRC28-10 × UAS-IVS-GFP-p10 vector (Addgene Plasmid, cat. no. 36431). The primer pairs used for PCR validation were as following:
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bioRxiv - Neuroscience 2021Quote: ... Synaptophysin1-mCherry plasmid was generated by replacing the pHluorin-tag in Synaptophysin1-pHluorin (gift from L. Lagnado, Addgene plasmid # 24478, (Granseth et al, 2006)) with mCherry from pmCherry-N1 (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: Human PUM1 full-length cDNA was amplified by PCR and subcloned in a pRK5 plasmid containing the Myc tag sequence (Addgene, pRK5-Myc-Parkin #17612) at the N-terminal by using SalI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... RBM41 C-terminal fragment (259–413) and full-length 65K were cloned into MAC-tag-N vector (Addgene #108078, a gift from Markku Varjosalo) using Gateway cloning as described (Liu et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... The TaDa Dam-only plasmid was generated by In-fusion cloning of mNeonGreen and E.coli Dam methylase into a linearized plasmid pHR-SFFV-dCas9-BFP-KRAB (a gift from Stanley Qi & Jonathan Weissman, Addgene plasmid # 46911), with MluI and SbfI restriction enzymes ...
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bioRxiv - Cell Biology 2023Quote: His-tagged Set9 protein expression was induced overnight at room temperature with 1 mM IPTG using BL21(DE3) Escherichia coli transformed with pET28 Set9 plasmid (Addgene plasmid #24082). Cells were pelleted at 7000 rpm for 20 minutes at 4°C using rotor JA-10 ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells using the plasmids pRSFDuet-1 His6-SUMO-CoiA1_CoiB and pETDuet-1 Coi_CoiSA(ED) (Addgene ID #208761 and #208762), following the method previously reported.10 For the preparation of methyllanthionine standard ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Developmental Biology 2022Quote: ... and AGO2 ORFs (p3XFLAG-myc-CMV-AGO2, gift from Dr. E. Chan) (Lian, Li et al., 2009) were obtained from Addgene (#50360 and #21538, respectively). For BiFC assays ...
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bioRxiv - Cell Biology 2021Quote: For CRISPR-mediated SERPINE1 knockout and transactivation the following lentivectors were used: pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE (Addgene 139086, Legut et al, 2020) and pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE (Addgene 139090 ...
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bioRxiv - Physiology 2019Quote: ... with a hairpin sequence: CCT AAG GTT AAG TCG CCC TCG CTC TAG CGA GGG CGA CTT AAC CTT AGG (Addgene plasmid 1864, Cambridge, MA, USA). Oligos were obtained from ITDDNA USA (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... a gift from Zuoshang Xu)63 and cloned in frame with the N-terminal 6- His-SUMO-TEV-site into the SspI site of pET 6His-SUMO-TEV LIC (1S) (Addgene 29659, a gift from Scott Gradia) using the HiFi Assembly kit (NEB) ...
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bioRxiv - Genetics 2019Quote: ... PRDM9 with a C-terminal V5 or N-terminal YFP tag for expression in mammalian cells (pLENTI CMV/TO Puro DEST backbone vector, Addgene plasmid # 17293; Campeau et al., 2009) were described previously (Altemose et al. ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells transiently co-expressing individual GFP-tagged RUSH reporter and GalT-mCherry were cultured in complete DMEM supplemented with16 nM His-tagged streptavidin (in-house purified using Addgene #20860, a gift plasmid from A. Ting)(Howarth et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and subcloned in the pBabe-puro GFP-N-term pCDNA-T0-FRT-Streptag (41) and pSBbi-PUR Sleeping Beauty-based expression vectors (Addgene #6023, a gift from E. Kowarz) (42) ...
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bioRxiv - Neuroscience 2021Quote: Plasmids containing the Escherichia coli MscL sequence in the WT form and with the G22S mutation were obtained from Francesco Difato (Addgene plasmids #107454 and #107455)56 ...