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• Suppression of piriform cortex alters brain-wide dynamics and alleviates seizures in temporal lobe epilepsy

  Huiqi Zhang, et al., bioRxiv - Neuroscience 2023

  Quote: ... or AAV-hSyn-DIO-mCherry (400 nl at titer 7×1012, Addgene, #50459-AAV5) as control ...
• Suppression of piriform cortex alters brain-wide dynamics and alleviates seizures in temporal lobe epilepsy

  Huiqi Zhang, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV-hSyn-DIO-hM3D(Gq)-mCherry (400 nl at titer 7×1012, Addgene, #44361-AAV5) for activation ...
• Suppression of piriform cortex alters brain-wide dynamics and alleviates seizures in temporal lobe epilepsy

  Huiqi Zhang, et al., bioRxiv - Neuroscience 2023

  Quote: ... or AAV-hSyn-DIO-hM4D(Gi)-mCherry (400 nl at titer 7×1012, Addgene, #44362-AAV5) for inhibition ...
• Trans-synaptic molecular context of NMDA receptor nanodomains

  Michael C Anderson, et al., bioRxiv - Neuroscience 2023

  Quote: ... psPAX2 (Addgene plasmid #12260) and pMD2.G (Addgene plasmid #12259 ...
• Trans-synaptic molecular context of NMDA receptor nanodomains

  Michael C Anderson, et al., bioRxiv - Neuroscience 2023

  Quote: ... and pMD2.G (Addgene plasmid #12259) were gifts from Didier Trono ...
• Trans-synaptic molecular context of NMDA receptor nanodomains

  Michael C Anderson, et al., bioRxiv - Neuroscience 2023

  Quote: ... pFUGW spCas9 (Addgene plasmid #131506) and pFUGW mCherry-KASH (Addgene plasmid #131505 ...
• Circuit mechanism underlying fragmented sleep and memory deficits in 16p11.2 deletion mouse model of autism

  Ashley Choi, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV-DIO-tdTomato (0.3 ul, #28306-AAV2, Addgene) was injected into LC ...
• Trans-synaptic molecular context of NMDA receptor nanodomains

  Michael C Anderson, et al., bioRxiv - Neuroscience 2023

  Quote: ... and pFUGW mCherry-KASH (Addgene plasmid #131505) were gifts from Harold MacGillavry ...
• A thalamic hub of action cues coordinates early visual processing and perception

  T. Vega-Zuniga, et al., bioRxiv - Neuroscience 2023

  Quote: Calcium indicator expression in vLGN/IGL neurons was achieved with AAV-hSynapsin1- FLEx-axon-GCaMP6s (1 × 1012 genome copies per ml, Addgene, 112010-AAV5) for retinal expression AAV2.7M8-syn-GCaMP8m viral vectors (1 × 1013 genome copies per ml) ...
• A thalamic hub of action cues coordinates early visual processing and perception

  T. Vega-Zuniga, et al., bioRxiv - Neuroscience 2023

  Quote: ... Tetanus Neurotoxin Light Chain (TeLC) viruses were generated using AAV5-hSyn-FLEX-TeLC-P2A-dTomato (Addgene, 159102, 1 × 1013 genome copies per ml) at ISTA viral facility ...
• A thalamic hub of action cues coordinates early visual processing and perception

  T. Vega-Zuniga, et al., bioRxiv - Neuroscience 2023

  Quote: Anterograde transsynaptic expression was done with AAV1-cre (AAV1.CamKII0.4.Cre.SV40, 7×10¹² vg/mL, Addgene). Retrograde transsynaptic expression was performed with starter vector (AAV-DIO-Ef1a-TVA-FLAG-2A-N2C_G ...
• Molecular tuning of sea anemone stinging

  Lily S He, et al., bioRxiv - Physiology 2023

  Quote: ... Lipscombe (RRID: Addgene_26578) and cacnb2a was a gift from A ...
• Molecular tuning of sea anemone stinging

  Lily S He, et al., bioRxiv - Physiology 2023

  Quote: ... Rat cacna2d1 (RRID: Addgene_26575) and cacna1a were gifts from D ...
• Molecular tuning of sea anemone stinging

  Lily S He, et al., bioRxiv - Physiology 2023

  Quote: ... Dolphin (RRID: Addgene_107424).
• Bi-allelic variants in WDR47 lead to neuronal loss causing a rare neurodevelopmental syndrome with corpus callosum dysgenesis in humans

  Efil Bayam, et al., bioRxiv - Neuroscience 2023

  Quote: ... method into pDONR221 entry vector and then recombined into a yeast low-copy number CEN destination vector (Addgene, 70) to obtain pAG413-promGPD-WDR47 (pSF634) ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: Plasmid p L4665 (a gift from Andrew Fire, Addgene #1667) carrying the C ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: The sequences encoding STIM1 and RspA-NFAST were amplified by PCR from respectively the human STIM1-YFP plasmid (Addgene #19754, a gift from A. Rao) and the pAG573 FRB-RspA(N)-IRES-mTurquoise214 plasmid ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... LifeActGFP (Addgene #58470).
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following plasmids were from Addgene: ER-StayGold (Addgene #186296) ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... OMM-CaM*-CFAST10 was generated by amplifying by PCR the sequence encoding mutated Calmodulin (CaM*) from the plasmid pCMV CEPIA3mt (Addgene #58219). The CaM*-encoding fragment was then ligated between the OMM-targeting sequence and the CFAST10-encoding sequence in the OMM-short-CFAST10 plasmid described above.
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... mCherry-Drp1 (Addgene #49152), Tubuline-GFP (Addgene #56450) ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... the M13-encoding sequence was amplified by PCR from the plasmid pCMV CEPIA3mt (Addgene #58219) and ligated between the ER-membrane targeting sequence and the NFAST-encoding sequence in the above described ER-NFAST plasmid ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... the pCAG:myr-Venus sequence (#32602, Addgene, Watertown, MA, USA) was amplified by PCR using the following primers (Integrated DNA Technologies ...
• Ribosome Quality Control Mechanism Mitigates the Cytotoxic Impacts of Ribosome Collisions Induced by 5-Fluorouracil

  Susanta Chatterjee, et al., bioRxiv - Cell Biology 2023

  Quote: The pSpCas9(BB)-2A-GFP (Addgene, Cat. 48138) plasmid was linearized using BbsI (Thermo Fisher Scientific ...
• The Neuroprotective Effect of Short-chain Fatty Acids Against Hypoxia-reperfusion Injury

  Anjit K. Harijan, et al., bioRxiv - Neuroscience 2023

  Quote: ... PCDNA3-FlipGFP (Casp3 cleavage seq) T2A mCherry obtained from Addgene (Catalogue ID 124428). Cobalt(II ...
• Oncogenic PIK3CA corrupts growth factor signaling specificity

  R.R. Madsen, et al., bioRxiv - Systems Biology 2023

  Quote: The BTK-PH domain was obtained from Addgene construct #51463 (a gift from Dr Tamas Balla) ...
• Improving the power of drug toxicity measurements by quantitative nuclei imaging

  Alesya M. Mikheeva, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Lentiviral preparation was performed as described previously using pLentiPGK Hygro DEST H2B-mRuby2 (Addgene #90236)49 and then cells were transduced to achieve at least 30% transduction rate ...
• All-optical reporting of inhibitory receptor driving force in the nervous system

  Joshua S. Selfe, et al., bioRxiv - Neuroscience 2023

  Quote: ... which was a gift from Mingshan Xue (Addgene plasmid #114369) (Messier et al. ...
• All-optical reporting of inhibitory receptor driving force in the nervous system

  Joshua S. Selfe, et al., bioRxiv - Neuroscience 2023

  Quote: ... which was a gift from Karl Deisseroth (Addgene plasmid #55647) (Fenno et al. ...
• Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

  Louis Richevaux, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5.Syn.ChrimsonR-tdTomato.WPRE.bGH (AAV5-Chrimson, Penn Vector Core, Addgene 59171P) induced neuronal expression of the red light gated channelrhodopsin Chrimson fused to the tdTomato marker ...
• Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

  Louis Richevaux, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5.Syn.Chronos-GFP.WPRE.bGH (AAV5-Chronos, Penn Vector Core, Addgene 59170P) was used to induce neuronal expression of the blue light induced channelrhodopsin Chronos fused to the GFP marker and under the control of the Synapsin promoter ...
• Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

  Louis Richevaux, et al., bioRxiv - Neuroscience 2023

  Quote: Projecting neurons were labeled with retrograde fluorescent tracers (Retrobeads, Lumafluor, and pAAV-CAG-tdTomato, Addgene 59462P). Fluorescent beads were stored at 4°C before use ...
• Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes

  Bruno A. Cisterna, et al., bioRxiv - Cell Biology 2023

  Quote: ... pMDLg/pRRE (Addgene #12251), and pLentiCRISPRv2-PFN1sgRNA (500 ng each ...
• Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes

  Bruno A. Cisterna, et al., bioRxiv - Cell Biology 2023

  Quote: The following DNA constructs (Addgene) were used in this study ...
• Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes

  Bruno A. Cisterna, et al., bioRxiv - Cell Biology 2023

  Quote: ... pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
• Non-canonical contribution of human Dicer helicase domain in antiviral innate immune response

  Morgane Baldaccini, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 0.33 µg of the pVSV envelope plasmid (Addgene #8454) and 1.33µg of pSPAX2 packaging plasmid (Addgene #12260 ...
• Non-canonical contribution of human Dicer helicase domain in antiviral innate immune response

  Morgane Baldaccini, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and 1.33µg of pSPAX2 packaging plasmid (Addgene #12260) using Lipofectamine 2000 reagent (Invitrogen ...
• An AAV capsid reprogrammed to bind human Transferrin Receptor mediates brain-wide gene delivery

  Qin Huang, et al., bioRxiv - Neuroscience 2023

  Quote: ... macaque: NM_001257303.1) into the lentiviral backbone pLenti-EF-FH-TAZ-ires-blast (Addgene #52083) with EcoRI/SalI sites.
• An AAV capsid reprogrammed to bind human Transferrin Receptor mediates brain-wide gene delivery

  Qin Huang, et al., bioRxiv - Neuroscience 2023

  Quote: ... the coding sequence of the extracellular region of human TfR1 (residues 89–760) were amplified by PCR and inserted into pCMV6-XL4 FLAG-NGRN-Fc (Addgene #115773) with EcoRV and XbaI sites ...
• Diversity and connectivity of principal neurons in the lateral and basal nuclei of the mouse amygdala

  Zsófia Reéb, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAVrg-CAG-GFP (Addgene, 37825-AAVrg ...
• Diversity and connectivity of principal neurons in the lateral and basal nuclei of the mouse amygdala

  Zsófia Reéb, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5-CAMKII-eGFP (Addgene 50469-AAV5 ...
• An AAV capsid reprogrammed to bind human Transferrin Receptor mediates brain-wide gene delivery

  Qin Huang, et al., bioRxiv - Neuroscience 2023

  Quote: ... The desired region of plasmid pCMV6-XL4 FLAG-NGRN-Fc (Addgene #115773) was amplified by PCR ...
• An AAV capsid reprogrammed to bind human Transferrin Receptor mediates brain-wide gene delivery

  Qin Huang, et al., bioRxiv - Neuroscience 2023

  Quote: ... and pVSV-G) and vector plasmid (pLenti-EF-FH-TAZ-IRES-blast, a gift from Yutaka Hata, Addgene plasmid # 52083) encoding the entire coding sequences of TFRC or Tfrc (human ...
• Precise Therapeutic Targeting of Distinct NRXN1^+/- Mutations

  Michael B. Fernando, et al., bioRxiv - Neuroscience 2023

  Quote: ... hiPSCs were instead co-transduced with TetO-Ascl1-puro (Addgene #97330) or TetO-Ascl1-neo (Addgene #TBD) ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... Teto-DLX2-hygro (Addgene catalog no. 97330) and FUdeltaGW-rtTA (Addgene ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... and NGN2 (Addgene 99378) lentiviruses ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... NPCs were transduced with rtTA (Addgene 20342) and NGN2 (Addgene 99378 ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... as above) hiPSC-derived NPCs expressing dCas9-KRAB for CRISPRi were transduced with rtTA (Addgene 20342) and NGN2 (Addgene 99378 ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... and NGN2 (Addgene 99378) lentiviruses and grown as described above ...
• Common genetic variation impacts stress response in the brain

  Carina Seah, et al., bioRxiv - Genetics 2023

  Quote: ... pools of mixed gRNA vectors (Addgene 99374), either targeting relevant genes or scramble controls ...