-
No products found
because this supplier's products are not listed.
Geoffrey L. Rogers, et al.,
bioRxiv - Immunology 2023
Quote:
... 1 µg of His-tagged HIV-1 JR-CSF gp120 (Immune Technology) was added to cells for 15 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2020
Quote:
Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
-
No products found
because this supplier's products are not listed.
Dana L.E. Vergoossen, et al.,
bioRxiv - Immunology 2020
Quote:
... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
-
No products found
because this supplier's products are not listed.
Andrew J. Suh, et al.,
bioRxiv - Cell Biology 2023
Quote:
... gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (Catalog number 40591-V08H ...
-
No products found
because this supplier's products are not listed.
Alan D. Curtis, et al.,
bioRxiv - Immunology 2021
Quote:
... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
-
No products found
because this supplier's products are not listed.
Zach Troyer, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488). Antibodies were diluted in a blocking buffer provided in the kit ...
-
No products found
because this supplier's products are not listed.
Kurtis Stefan, Artem Barski,
bioRxiv - Genomics 2022
Quote:
... and pLAI-HIV (Addgene #133996)] ...
-
No products found
because this supplier's products are not listed.
Swati Jain, et al.,
bioRxiv - Microbiology 2023
Quote:
... GnTi expressed His-tagged gp140s and gp120s were purified from the harvested and clarified supernatant using Ni-NTA agarose beads (Qiagen) following manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Mher Garibyan, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... HEK293 cells (Takara 632180), C3H/10T1/2 Clone 8 (ATCC# CCL-226) ...
-
No products found
because this supplier's products are not listed.
Mona Rheinberger, et al.,
bioRxiv - Genomics 2022
Quote:
... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
-
No products found
because this supplier's products are not listed.
Anthony J. Rabe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The hexahistidine tag and Sso7dmut were cleaved from HIV-1 IN by addition of his-tagged sortase and GGGC peptide (GenScript). The reaction was exposed to nickel resin to remove sortase and any residual uncleaved IN ...
-
No products found
because this supplier's products are not listed.
Tzu-Hsuan Chang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
No products found
because this supplier's products are not listed.
Nicholas McCaul, et al.,
bioRxiv - Biochemistry 2021
Quote:
... glycans were removed from lysate-derived gp120 or gp160 with Endoglycosidase H (Roche) treatment of the immunoprecipitates as described before (Land et al. ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The HIV-1NL4.3 LTR was cloned into pG5luc (Promega) upstream of the firefly luciferase gene ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Shayan G. Borhani, et al.,
bioRxiv - Bioengineering 2022
Quote:
... recombinant HIV-1 Gp120 was immobilized on high-binding ELISA plates (Greiner #655081). Plates were washed with PBS-T and blocked with a solution of 5% (w/v ...
-
No products found
because this supplier's products are not listed.
Chuan Xu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
-
No products found
because this supplier's products are not listed.
Jia Qin, et al.,
bioRxiv - Epidemiology 2019
Quote:
... such as HIV-1 Western Blot (Genetic Systems HIV-1 Western Blot, Bio-Rad Laboratories, Redmond, Washington, US) and/or an immunofluorescent antibody (IFA ...
-
No products found
because this supplier's products are not listed.
Christian L. Egly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... All HEK293 cells were cultured in HEK293 media consisting of Minimum Essential Media (MEM, Corning) containing 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
-
No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... NL4-3 and NL4-3(AD8) HIV viral stocks were produced by transfection of HEK293 cells with the molecular clone plasmids using TransIT-LT1 (Mirus) transfection reagent ...
-
No products found
because this supplier's products are not listed.
I Sánchez-Cerrillo, et al.,
bioRxiv - Immunology 2024
Quote:
... mouse anti-HIV-1 P24 (Dako), goat anti-human TRAIL (RyD Systems ...
-
No products found
because this supplier's products are not listed.
Andi Pan, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 5 μg gp120 was adjuvanted with 10 μg monophosphoryl lipid A (InvivoGen, vac-mpla) and 10 μg saponin (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Nicholas McCaul, et al.,
bioRxiv - Biochemistry 2021
Quote:
... HeLa cells were transfected with pMQ gp120/gp160 or HA constructs using polyethylenimine (Polysciences) as described before (Hoelen et al. ...
-
No products found
because this supplier's products are not listed.
Lachlan P. Deimel, et al.,
bioRxiv - Immunology 2023
Quote:
... wherein biotinylated gp120 was tetramerised with PE-conjugated strepdavidin (Biolegend).
-
No products found
because this supplier's products are not listed.
John A. Frank, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Transfected cells were infected with reporter virus by applying virus (HIV-RD114env, HIV-VSVg, HIV-SMRVenv) stocks in the presence of polybrene (Santa Cruz Bio, sc-134220) at a final concentration of 4 ug/mL ...
-
No products found
because this supplier's products are not listed.
Ross D Overacker, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Inhibition of HIV-1 integrase was assessed using an HIV-1 integrase assay kit (XpressBio Life Science Products) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Chloé Colas, et al.,
bioRxiv - Immunology 2022
Quote:
... integrated HIV DNA and unspliced HIV RNA was performed by modified nested real-time PCR assay using Taq DNA polymerase (BioLabs) in the first PCR and TaqMax Fast Advanced Master Mix (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Ying Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified HIV particles were fixed using by BD CytoPerm/Cytofix Solution and adhered to poly-L-lysine solution (SIAMG ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Allison Ballandras-Colas, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
-
No products found
because this supplier's products are not listed.
Neil D. Butler, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech. Oligonucleotides were purchases from IDT ...
-
No products found
because this supplier's products are not listed.
Paradise Madlala, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... individuals testing HIV negative by rapid immunoassays (SD Bioline HIV1/2 Elisa 3.0 ...
-
No products found
because this supplier's products are not listed.
Noelle Anderson, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... and sequencing (Illumina Hi-Seq) were performed by Iridian Genomes ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Catherine M. Card, et al.,
bioRxiv - Immunology 2020
Quote:
Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
-
No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... HEK293/GC-A+ and HEK293/GC-B+ cell lines were generated from HEK293 parental cell transfected with plasmids (OriGene, Rockville, MD) containing either human GC-A or GC-B cDNA sequences ...
-
No products found
because this supplier's products are not listed.
Jun Cao, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lenti-Pac™ HIV Expression Packaging Kit (GeneCopoeia) was used for transfection in 293T cell line following the instructions of the kit ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Suwellen S. D. de Azevedo, et al.,
bioRxiv - Immunology 2019
Quote:
... and the Abbott RealTime HIV-1 assay (Abbott Laboratories ...
-
No products found
because this supplier's products are not listed.
Alexandra Maslennikova, et al.,
bioRxiv - Microbiology 2021
Quote:
The levels of HIV-1 core protein Gag in cell supernatants were quantified using HIV-1 p24 ELISA Kit (Zeptometrix, Bufalo NY, USA) in accordance to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Philipp Kolb, et al.,
bioRxiv - Microbiology 2020
Quote:
... αHis-PE (Miltenyi Biotec). Cytotect® (CP Biotest) ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...