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Citations for PerkinElmer :
1 - 50 of 50 citations for gp120 HIV HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Immunology 2023Quote: ... Pelleted particles were resuspended in D10 medium and the concentration of HIV p24 was quantitated by using an HIV-1 p24 ELISA Kit (Perkin Elmer, Boston, MA, USA) [44 ...
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bioRxiv - Molecular Biology 2019Quote: ... The DuPont HIV-1 p24 Core Profile ELISA kit (Perkin Elmer, Waltham ...
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bioRxiv - Immunology 2021Quote: ... Viral titers were quantified by HIV-1 p24 AlphaLISA assay (PerkinElmer; AL291F) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Viral stocks were titrated using HIV-1 P24 ELISA assay (#NEK05001KT, Perkin Elmer) and the concentration was 200 ng/ml.
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Immunology 2019Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
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bioRxiv - Microbiology 2023Quote: ... 0.45 µm-filtered and used for HIV-1 CAp24 quantification by ELISA (released CAp24) (Perkin Elmer). Cell lysates were analyzed by western blotting.
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293-ACE2 cells were seeded in 384-well white plates (PerkinElmer, USA), allowed to attach overnight followed by addition of pseudoviral particles in the presence or absence of varying concentrations of GRFT ...
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bioRxiv - Microbiology 2021Quote: ... Gag p24 antigen concentration was measured with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Molecular Biology 2021Quote: ... Levels of HIV-1 gag p24 in culture supernatants were measured by ELISA as instructed by manufacturer (PerkinElmer).
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bioRxiv - Microbiology 2021Quote: Concentration of HIV in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2022Quote: ... by measuring the Gag p24 antigen concentration with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Replication-competent HIV-1 stocks were normalized by p24Gag levels determined by ELISA (PerkinElmer Life Sciences, Inc., Boston, MA, USA).
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells stably transfected with LRRK2 plasmids were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).