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Citations for Bio-Rad :
1 - 50 of 71 citations for gp120 HIV HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Epidemiology 2019Quote: ... such as HIV-1 Western Blot (Genetic Systems HIV-1 Western Blot, Bio-Rad Laboratories, Redmond, Washington, US) and/or an immunofluorescent antibody (IFA ...
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bioRxiv - Microbiology 2021Quote: ... HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (Rutsaert et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Immunology 2019Quote: HIV antigens were covalently conjugated to polystyrene beads (Bio-Rad), and binding of IgG to the bead-conjugated HIV-1 antigens was measured in RM plasma samples (29) ...
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bioRxiv - Immunology 2019Quote: ... HIV-1 antigens were conjugated to polystyrene beads (Bio-Rad) as previously described (18) ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Microbiology 2023Quote: HIV-1/O RNA was quantified on a CFX96 Deep-Well (BioRad) by targeting integrase O thanks to primers and TaqMan probe ...
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bioRxiv - Cell Biology 2021Quote: ... for detecting HIV-1 reverse transcription or iQ SYBR Green Supermix (Bio-Rad) for detecting actin.
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bioRxiv - Immunology 2019Quote: ... HIV-1 antigens were covalently coupled to carboxylated fluorescent beads (Bio-Rad Laboratories) and IgG binding to the bead-coupled antigens was measured ...
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Cell Biology 2021Quote: qPCR for HIV-1 TAR and envelope was carried out with IQ Supermix (Bio-Rad). The following primers/probes were used ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: ... All patients were HIV tested using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Immunology 2021Quote: ... HIV DNA copy number was evaluated using the QX-100 Droplet Digital PCR system (Bio-Rad, Hercules, CA). The PCR-positive and PCR-negative droplets for HIV gag and RPP30 were determined and template concentrations were calculated by Poisson analysis ...
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Microbiology 2021Quote: ... or a median 588ng (IQR 488-697ng) (HIV) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900nM ...
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bioRxiv - Microbiology 2023Quote: ... or a median 750 ng (for HIV-1) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900 nM ...
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bioRxiv - Microbiology 2023Quote: HIV-1/N and M strains were quantified by targeting the long terminal repeat (LTR) region using GENERIC HIV Charge virale kit (Biocentric) performed on CFX96 Deep-Well (Biorad) with the followed program RT 10 min at 50°C and 5 min at 95°C ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
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bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
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bioRxiv - Genomics 2019Quote: ... Total HIV-1 DNA content of CD4 T-cell DNA isolates was measured by digital droplet PCR (ddPCR, QX200 platform, Bio-Rad, Temse, Belgium), as described by Rutsaert et al.56 The DNA was subjected to a restriction digest with EcoRI (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Individuals with a positive HIV-1 RNA test and a negative HIV-1 ELISA test (at the time of first RNA positive test) and an evolving Western blot (GS HIV Type 1 Western blot, Bio-Rad, Redmond, WA, USA) pattern indicative of a recent HIV-1 infection were enrolled ...
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bioRxiv - Cell Biology 2023Quote: ... Vector titers were determined by calculating the ratio between the copies of HIV psi and every two copies of RPP30 via QX200 digital droplet PCR system (Bio-Rad, Carlsbad, CA, USA), multiplied by the number of cells transduced and if necessary ...
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
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bioRxiv - Immunology 2021Quote: ... Viral stocks were normalized by p24-Gag content as measured with an Enzyme Linked Immunosorbent Assay test (GenscreenTM HIV-1 Ag Assay; Bio-Rad, Marnes-la-Coquette, France). Virions were used to infect CEM.NKR-CCR5 cells after ELISA-p24 quantification and normalization ...
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bioRxiv - Bioengineering 2024Quote: ... gene and the HIV-1 Psi vector sequence were performed using respectively custom qPCR HEX assay and custom qPCR FAM assay (Bio-Rad, Marnes-la-Coquette, France) with previously published primer sequences.48 qPCR reactions were performed using 200 ng of gDNA and the Master Mix Taqman Universal II (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
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bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...