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No products found
because this supplier's products are not listed.
Pablo Martin-Baniandres, et al.,
bioRxiv - Biochemistry 2023
Quote:
Trx-linker concatemers (1 mg/mL) were incubated with 50,000 units of the catalytic subunit of cAMP-dependent protein kinase (PKA) (NEB)—which recognizes the RRAS motif within the central linker of the Trx-linker nonamer—in protein kinase buffer (50 mM Tris HCl ...
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No products found
because this supplier's products are not listed.
Laura Morano, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... anti-DNA-dependent protein kinase catalytic subunit (DNA-PKcs) phosphorylated at S2056 (Abcam, #ab124918). The antibody against H2AX phosphorylated at S139 (Abcam ...
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No products found
because this supplier's products are not listed.
Sabine Ruegenberg, et al.,
bioRxiv - Biochemistry 2020
Quote:
Protein Kinase A Catalytic Subunit from bovine heart (PKA, EC 2.7.11.11, Sigma) was reconstituted in bi-distilled water containing 6 mg/ml DTT at a concentration of 50 μg/ml ...
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No products found
because this supplier's products are not listed.
Muhammed Jamsheer K, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and incubated with 1.5 μg anti-SnRK1α1 antibody (SNF1-related protein kinase catalytic subunit alpha KIN10 antibody; catalog no.: AS10 919, Agrisera) in 600 μL PBS on a rotator shaker for 1 h at room temperature with gentle shaking ...
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No products found
because this supplier's products are not listed.
Jennifer M. Myers, et al.,
bioRxiv - Immunology 2024
Quote:
... PKA activation in H9c2 cells was measured using SignaTECT cAMP-dependent protein kinase assay system (Promega) according to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Alican Kuşoğlu, et al.,
bioRxiv - Bioengineering 2023
Quote:
Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
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No products found
because this supplier's products are not listed.
Jasper Che-Yung Chien, et al.,
bioRxiv - Genetics 2020
Quote:
... DNA-dependent protein kinase catalytic subunit (DNA-PKcs) antibody was obtained from Santa Cruz (sc-5282, Dallas, TX, USA) and anti-phosphorylated DNA-PKcs was purchased from Abcam (ab124918 ...
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No products found
because this supplier's products are not listed.
Carl-Christian Kolbe, Eicke Latz,
bioRxiv - Biochemistry 2020
Quote:
... Final kinase concentration was 12.5 ng/µl for PRKACA (Thermo Fisher; Cat. # P2912), RPS6KA5 (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Nontawat Chuinsiri, et al.,
bioRxiv - Physiology 2023
Quote:
... a specific inhibitor targeting the alpha subunit of the Gi/o protein (Merck); U73122 ...
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No products found
because this supplier's products are not listed.
Alexis Riché, et al.,
bioRxiv - Plant Biology 2023
Quote:
... β subunit of ATPsynthase AtpB and STT7 kinase as well as anti-phosphothreonine antibodies (Cell Signaling) were used for immunodetection by ECL.
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No products found
because this supplier's products are not listed.
Jakob Mitgau, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the Alpha Screen cAMP assay kit (PerkinElmer) was used according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Zhaoqi Li, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-Pyruvate Dehydrogenase E1-alpha subunit (total) (Proteintech, 18068-1-AP), MPC2 (Cell Signaling Technologies ...
-
No products found
because this supplier's products are not listed.
Wen Li, et al.,
bioRxiv - Physiology 2020
Quote:
Recombinant human Mfge8 and recombinant human laminin subunit alpha-1 were purchased from R&D Systems, www.rndsystems.com ...
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No products found
because this supplier's products are not listed.
Jawad S Khalil, et al.,
bioRxiv - Cell Biology 2023
Quote:
... IgG2b clone 18), IIα (612243, IgG1 clone 40), IIβ (610626, IgG1 clone 45), and catalytic-α subunit (610980, IgG2b clone 5B) from BD biosciences (Wokingham ...
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No products found
because this supplier's products are not listed.
Timothy A. Dinh, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
-
No products found
because this supplier's products are not listed.
Viola Vaňková Hausnerová, et al.,
bioRxiv - Microbiology 2024
Quote:
... Proteins were detected by western blotting using an anti-RNAP β subunit antibody [clone 8RB13] (BioLegend), anti-σ70 antibody [clone 2G10] (BioLegend) ...
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No products found
because this supplier's products are not listed.
Pankaj Sharma, et al.,
bioRxiv - Immunology 2023
Quote:
... and hemagglutinin stalk protein (HA2 subunit) were purchased from Sino Biologicals Inc (Beijing ...
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No products found
because this supplier's products are not listed.
A. Sanluis-Verdes, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Arylphorin subunit alpha-like (Demetra) and hexamerin (Ceres) were produced by Genscript, utilizing the baculovirus expression system in insect cells ...
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No products found
because this supplier's products are not listed.
Anisha D’Souza, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Recombinant Human Adenosine triphosphate synthase subunit alpha (ATP5A)-Glutathione S-transferase (GST) (N-term) full- length recombinant protein was obtained from Novus Biologicals Inc ...
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No products found
because this supplier's products are not listed.
Lourds M. Fernando, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... a mouse monoclonal proteasome 20S core particle antibody that recognizes alpha 1,2,3,4,5,6,7 subunits (1:200, Enzo Life Sciences, Cat. No. BML-PW8195) and rabbit GFP antibody (1:500 ...
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No products found
because this supplier's products are not listed.
Alexandre P. Martin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and APEX2-dependent protein biotinylation by Western blotting using Streptactin-HRP (Bio-Rad). A similar strategy and gRNA were used to insert a HaloTag at the C-terminus of endogenous Notch2.
-
No products found
because this supplier's products are not listed.
Yuki Yoshida, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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No products found
because this supplier's products are not listed.
Joseph M. Varberg, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and thiamine-dependent repression was validated by immuno blotting of whole cell extracts using anti-HA antibodies (Roche, 3F10).
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No products found
because this supplier's products are not listed.
Catherine W. Cai, et al.,
bioRxiv - Immunology 2020
Quote:
... and B6.129S6-Cybbtm1Din/J mice lacking the gp91phox catalytic subunit of NADPH oxidase (gp91phox KO, The Jackson Laboratory) were obtained directly from the vendor or maintained as breeding colonies within the Hoft laboratory ...
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No products found
because this supplier's products are not listed.
Émilie Lavallée, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and PRKACA (GS5566) were purchased from Qiagen (Hilden, DEU). Cells were transfected with Lipofectamine RNAiMAX transfection reagent (#13778500 ...
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No products found
because this supplier's products are not listed.
Min Jung Kwon, et al.,
bioRxiv - Neuroscience 2021
Quote:
... dibutyryl-cAMP (db-cAMP; 100⎧M; Calbiochem) and 100 ⎧g/ml of recombinant ONCM ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex). Antibody binding was detected with goat anti-mouse IgG mAb conjugated with AlexaFluor 647 fluorochrome (Sigma) ...
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No products found
because this supplier's products are not listed.
Erwan Lambert, et al.,
bioRxiv - Neuroscience 2021
Quote:
... anti-NA/K ATPase alpha subunit (a5, DSHB, 1/100), anti-rhodopsin (4C5, DSHB, 1/200) and anti-GFP (132004, Synaptic System, 1/100). After washing they were incubated overnight at 4°C with Alexa 555 Phalloidin anti-F-actin (A34055 ...
-
No products found
because this supplier's products are not listed.
Nashwa Cheema, Jessie M. Cameron, David A. Hood,
bioRxiv - Cell Biology 2021
Quote:
... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Cristian V. Crisan, et al.,
bioRxiv - Microbiology 2023
Quote:
... and secondary antibodies against rabbit (for Hcp) and mouse (for RNA polymerase subunit alpha) (1:5000, LI-COR Biosciences). The membrane was imaged using a Biorad ChemiDoc imager and analyzed in FIJI.
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No products found
because this supplier's products are not listed.
Apurva T. Prabhakar, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... CKII alpha’ Antibody 1:1000 (Bethyl; catalog no. A300-199A).
-
No products found
because this supplier's products are not listed.
Yu-Chen Yen, et al.,
bioRxiv - Biophysics 2023
Quote:
... was then mixed with d2-labeled cAMP and anti-cAMP cryptate-labeled antibody provided in the kit in a 384-well plate (Corning, REF3824). The plate was sealed and incubated at room temperature for 1 hour ...
-
No products found
because this supplier's products are not listed.
Alexandra Weiss, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Slides were then placed in secondary antibodies, Alpha-Bungarotoxin AF555 1:500 (ThermoFischer, #B35451) and Donkey anti-Rabbit AF488 1:450 (Jackson ImmunoResearch, #711-545-152), diluted in PBS1X for 2.5 hours at room temperature ...
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No products found
because this supplier's products are not listed.
Leyla Dogan, et al.,
bioRxiv - Bioengineering 2021
Quote:
... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
-
No products found
because this supplier's products are not listed.
I. Deniz Derman, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Alpha Tubulin (TUBA4A) mouse monoclonal antibody (Origene) and MUC5AC monoclonal antibody (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Pravin Kesarwani, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... followed by Tris base (pH 9.0) dependent protein antigen retrieval (Vector Labs, Burlingame, CA) at 95°C ...
-
No products found
because this supplier's products are not listed.
Madhusoodhanan Suresh Kumar Meena Kumari, et al.,
bioRxiv - Immunology 2024
Quote:
... IFN-I receptor subunit 1 (IFNAR1) blocking antibody (MAR1-5A3) and IgG isotype control antibody (MOPC-21) from BioXCell. S ...
-
No products found
because this supplier's products are not listed.
Nathan Hodson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and visualized using a Fluorochem E Imaging system (Protein Simple; Alpha Innotech, Santa Clara, CA). Bands were quantified using Protein Simple AlphaView SA software and normalized to Ponceau S and a gel control (identical generic sample run on every gel).
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No products found
because this supplier's products are not listed.
Zhenglin Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Pyruvate kinase M-2 (PKM2) (ABclonal, A16700), α-SMA (ABclonal ...
-
No products found
because this supplier's products are not listed.
Arnaud N’Guessan, et al.,
bioRxiv - Genomics 2022
Quote:
... and goat anti-human IgA (alpha chain) DyLight800 (Rockland Immunochemicals) antibodies at a final concentration of 0.1 μg/ml and 1 μg/ml ...
-
No products found
because this supplier's products are not listed.
Jenifer P. Suntharalingham, et al.,
bioRxiv - Genetics 2024
Quote:
... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
-
No products found
because this supplier's products are not listed.
Vinko Besic, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Rabbit polyclonal antibodies (PABPC1, Atlas Antibodies #HPA045423; p70 S6 kinase α (H-160), Santa Cruz #sc-9027 ...
-
No products found
because this supplier's products are not listed.
Koji Iwamoto, Satomi Matsuoka, Masahiro Ueda,
bioRxiv - Molecular Biology 2023
Quote:
... the concentration gradient of cAMP was generated using FemtoJet (Eppendorf): DB+ containing 10 μM cAMP was filled in a glass micropipette (Femtotips ...
-
No products found
because this supplier's products are not listed.
Vargas Alexandra, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and alpha-tubulin (Active Motif, 39527). Horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibodies were from Invitrogen ...
-
No products found
because this supplier's products are not listed.
Dorota Raj, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
-
No products found
because this supplier's products are not listed.
Harry Klein, et al.,
bioRxiv - Plant Biology 2021
Quote:
... anti-GT1 (1:75) and anti-YSPTSPS repeat S2Pho (RNA Pol II, B1 subunit; 1:200, Diagenode). The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20 ...
-
No products found
because this supplier's products are not listed.
Zane Kliesmete, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... Primary antibodies (chicken alpha-GFP, Aves Labs: GFP-1010 and rabbit alpha-Ki67 ...
-
No products found
because this supplier's products are not listed.
Monika Chodasiewicz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... cAMP binding assays to G-actin were performed with the Monolith NT.115 (Nanotemper, Munich, Germany) using 50 nM NTred-maleimid 2nd generation labelled G-actin and increasing concentrations of cAMP ...
-
No products found
because this supplier's products are not listed.
Laura Radić, et al.,
bioRxiv - Microbiology 2024
Quote:
Antibodies were loaded onto a protein A biosensor (Sartorius) for 300 s with a concentration of 10 μg/mL ...
-
No products found
because this supplier's products are not listed.
Wonkyung Oh, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... was coated with hPD-L1-His protein and anti-PD-L1 antibody and anti-mouse IgG specific HRP conjugated secondary antibodies (SouthernBiotech, Birmingham, AL, USA) were added ...