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Citations for Agilent :
1 - 50 of 2407 citations for cAMP dependent protein kinase catalytic subunit alpha PRKACA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
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bioRxiv - Physiology 2024Quote: ... Proline point mutations were made in the Nav1.5 alpha subunit with the QuikChange II site-directed mutagenesis kit (Agilent) and primers from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Catalytic mutants for SpokenTIMs were generated via QuikChange-PCR (Agilent) with templates from wild-type genes and proper mutation primers ...
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bioRxiv - Microbiology 2024Quote: ... to inactivate ExoS ADPRT catalytic activity following the protocol provided by Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). The melting temperature calculation was performed as described previously46.
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). Melting temperature shifts were calculated using the previously described method61.
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bioRxiv - Bioengineering 2024Quote: ... anti-alpha-SMA (Dako), anti-fibronectin (AbCam ...
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bioRxiv - Physiology 2021Quote: ... sections were immunostained with alpha-smooth muscle actin (α-SMA) antibody (Clone 1A4, DAKO, M0851 ...
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bioRxiv - Neuroscience 2020Quote: Site-directed insertions were made in GluN1 (GluN1-a) (NCBI Protein database accession no. P35439) or GluN2A (Q00959) subunits with the QuikChange site-directed mutagenesis kit (Agilent) with XL1-Blue super-competent cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cholesterol-dependent Permeabiliser XF Plasma Membrane Permeabiliser (PMP; Agilent) was used ...
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bioRxiv - Biophysics 2024Quote: ... Troponin subunits were then expressed in BL21(DE3)CodonPlus (Agilent) E ...
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bioRxiv - Cancer Biology 2023Quote: ... 70 kDa subunit (NF-L, 1:500, Agilent, Santa Clara, CA, #M0762). Immunostained sections were counterstained with hemalum.
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bioRxiv - Biophysics 2020Quote: The deletion construct containing the catalytic domain and the interdomain linker (CreCat, residues 127-343) was prepared using QuikChange Site-Directed Mutagenesis Kit (Agilent) from a pET21A vector (Novagen ...
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bioRxiv - Biochemistry 2024Quote: ... were introduced into all TE expression plasmids in place of codons for the catalytic Ser or Cys residues using the QuikChange XL Site-Directed Mutagenesis kit (Agilent), optimized for GC-rich sequences (JuvEV TE S1637amb ...
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bioRxiv - Cancer Biology 2022Quote: ... Estrogen Receptor alpha (ERα, Clone EP1, Dako IR 084), GATA3 (sc-268 ...
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bioRxiv - Physiology 2021Quote: ... sections were immunostained with alpha-smooth muscle actin (α-SMA) antibody (Clone 1A4, DAKO, M0851, Agilent Technologies, Santa Clara, CA, USA). Slides containing gastrocnemius muscle sections were deparaffinized in xylene and rehydrated using a graded ethanol series ...
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bioRxiv - Biophysics 2020Quote: Temperature-dependent turbidity assays were conducted using a UV-Vis spectrophotometer (Cary 100 Bio; Agilent) equipped with a multicell Peltier temperature controller ...
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bioRxiv - Biophysics 2023Quote: Temperature-dependent turbidity assays were conducted in a UV-Vis spectrophotometer (Cary 60 Bio; Agilent) equipped with a Peltier temperature controller ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-alpha Fetoprotein (AFP) (DAKO, Cat. # A0008; 1:300). Cardiomyocytes were immunostained with anti-α-Actinin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... monoclonal mouse anti-human alpha smooth muscle actin (M0851, Dako), monoclonal mouse anti-human CD68 (M0814 ...
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bioRxiv - Neuroscience 2020Quote: ... JNK3 kinase dead (K55R mutant) cDNA was generated by Quickchange (Stratagene). mCherry-tagged JIP3 in FEW vector was as previously described (Holland et al ...
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bioRxiv - Cancer Biology 2021Quote: ... estrogen receptor alpha (ERα) (clone:EP1, DAKO IR 084, ready-to-use), FOXA1 (Santa Cruz Biotechnology sc-6553 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-smooth muscle alpha-actin (α-SMA - Dako GA611, 1:100), anti-ACE2 (Merck SAB3500346 ...
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bioRxiv - Pathology 2024Quote: ... The fluorescence of SNARF-5F were measured in a time dependent manner using Synergy Neo2 (Agilent/BioTek) and the data analyzed offline as described previously (Wasano et al. ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against glial fibrillary acidic protein (GFAP) (Dako, Denmark) and for oligodendrocytes ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was visualized with a secondary HRP conjugated antibody (Dako) followed by development with ECL (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... Mutations were incorporated into AChR subunits using the Quickchange II site directed mutagenesis kit (Agilent Technologies, CA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... with the number of cycles dependent upon the outcome of quantification from a Bioanalyzer DNA 1000 chip (Agilent). We purified all samples post-capture using the MinElute PCR Purification Kit according to manufacturer specifications and eluted into 30 μL.
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bioRxiv - Molecular Biology 2022Quote: ... Generation of the kinase inactive variants was achieved using QuikChange PCR (Agilent).
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bioRxiv - Genomics 2024Quote: ... HLA-DR alpha-chain clone TAL.1B5 dilution 1:200 (M0746, Dako), CD68 clone PG-M1 dilution 1:100 (M0876 ...
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bioRxiv - Pathology 2024Quote: ... The fluorescence intensities of mVenusp.H148Q/p.I152L and mTq2 were simultaneously measured in a time-dependent manner using Synergy Neo2 (Agilent/BioTek) and the data analyzed offline as described previously (Wasano et al. ...
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bioRxiv - Biophysics 2023Quote: ... temperature-dependent turbidity assays were also conducted in the Schuster Lab in a UV-Vis spectrophotometer (Cary 3500; Agilent) equipped with a multicell Peltier temperature controller ...
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bioRxiv - Biochemistry 2023Quote: ... and Y402F Pyk2 FERM−kinase mutants were generated using the QuikChange (Agilent Genomics) site-directed mutagenesis strategy ...
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bioRxiv - Biochemistry 2022Quote: ... Pyk2 FERM– kinase mutants in pNAM004 were generated using the QuikChange (Agilent Genomics) site-directed mutagenesis strategy ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... 90 µl of ATP reagent was added to each well and ATP-dependent bioluminescence (RLU1) was measured after one minute as relative light units (RLU) by Synergy H1 plate reader (BioTek, Agilent). Two wells without cells were used as a blank (RLU0) ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific antibody binding was blocked by Serum-free protein block (Dako). Samples were incubated for 12 hr in a solution containing the following primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... membranes were probed with antibody recognizing core protein (Dako cat. no. B0586). For the detection of HBV DNA ...
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bioRxiv - Genomics 2023Quote: ... Non-specific antibody binding was blocked with Protein Block Serum-Free (Dako) for 10 minutes ...
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bioRxiv - Genetics 2022Quote: ... Glial Fibrillary Acidic Protein (GFAP) antibody (Z0334, Agilent Dako, 1:2000, rabbit), MAP2 antibody (188 004 ...
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bioRxiv - Genetics 2022Quote: ... Glial Fibrillary Acidic Protein (GFAP) antibody (Z0334, Agilent Dako, 1:2000, rabbit), MAP2 antibody (188 004 ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies: Glial fibrillary acidic protein (GFAP 1:100, DakoCytomation, cat. Z0334); S100 (β-subunit ...
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bioRxiv - Genetics 2023Quote: ... we inserted a wild-type histone array sequence containing the 5 replication-dependent histone genes into a pBluescript II KS+ vector (Agilent #212207) and introduced mutations using a Q5 Site-Directed PCR Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... or mVenusH148Q/I152L and mTq2 (for I-/Cl- antiport assay) were measured in a time dependent manner using Synergy Neo2 (Agilent/BioTek) and the data analyzed offline as described previously (Wasano et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Gα subunit plasmids were purchased from cdna.org (Bloomsburg University, Bloomsburg, PA. 4A insertion mutations were introduced to Gα subunits using the QuikChange Mutagenesis Kit (Agilent Technologies) and oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... rabbit glial fibrillary acidic protein antibody (anti-GFAP,1:500; Dako, Glostrup, Denmark), goat ionized calcium binding adaptor molecule 1 antibody (anti-Iba1,1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity number (RIN) was >8 for all samples (Agilent 1200 bioanalyzer, Alpha Metrix Biotech). Labeling of total RNA (100 ng ...