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Citations for Takara Bio :
1 - 50 of 698 citations for cAMP dependent protein kinase catalytic subunit alpha PRKACA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Plant Biology 2024Quote: Methylation-dependent restriction enzyme McrBC from TaKaRa (Code:1234A) and methylation-sensitive restriction enzyme PvuI from NEW ENGLAND BioLabs (R3150S ...
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bioRxiv - Cancer Biology 2020Quote: ... and T4 polynucleotide kinase (Takara). The extension reaction was performed with the AccuPower® DNA Sequencing Kit (Bioneer) ...
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bioRxiv - Developmental Biology 2020Quote: ... and catalytic core (residues 105–411) of SlPP2C3 were cloned into Y2H ‘prey’ vector pGADT7 (Clontech). Full-length SlPYLs and SlSnRK2s were cloned into the ‘bait’ vector pGBKT7 (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... ΔHEL2i and ΔHEL2 DICER and their catalytic mutants (CM) were generated by InFusion mutagenesis (Takara Bio) on pDONR-DICER and pDONR-DICER catalytic mutant vectors ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa) and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa), and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The α1-subunit genes were inserted at the PH promoter of vectors already containing the corresponding β1-subunit proteins using In-Fusion® HD Cloning Kit (Takara Bio, USA Inc.) and control sequenced ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2021: Escherichia coli DH5 alpha (Takara (Korea)) ...
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bioRxiv - Cell Biology 2021Quote: ... and then phosphorylated by T4 polynucleotide kinase (Takara, 2021S). pX459 was digested with BbsI ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by conducting a T4 polynucleotide kinase reaction (TAKARA). After phosphorylation ...
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bioRxiv - Genetics 2020Quote: ... 5′-phosphorylated by T4 polynucleotide kinase (TaKaRa, Shiga, Japan), self-ligated by T4 ligase (TaKaRa ...
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bioRxiv - Plant Biology 2023Quote: ... and end-labeled by T4 polynucleotide kinase (Takara Bio) and [γ-32P]-ATP (PerkinElmer) ...
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bioRxiv - Neuroscience 2023Quote: ... Red Fluorescent Protein Antibody (DsRed, 1:500, Clontech). The brain sections were incubated with antibodies in blocking solution at 4°C temperature overnight ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequent ligation using T4 polynucleotide kinase (Takara, Kusatsu, Japan) and T4 DNA ligase (Ligation mix #6023 ...
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bioRxiv - Genetics 2024Quote: ... and 1 μL of T4 Polynucleotide Kinase (10 U/μL; TAKARA) were added ...
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bioRxiv - Microbiology 2024Quote: ... The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase, Takara Bio), seven Ns and partial sequencing adapters were added in the primer as the barcode ...
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bioRxiv - Plant Biology 2020Quote: ... the YFP protein was detected using the GFP antibody (Clontech) at 1/5,000th and the UGPase antibody (Agrisera ...
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bioRxiv - Neuroscience 2023Quote: ... Green Fluorescent Protein (GFP) antibody (JL-8, 1:500, Clontech), Red Fluorescent Protein Antibody (DsRed ...
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bioRxiv - Plant Biology 2021Quote: ... Strands with a 5′ monophosphate were radiolabeled with T4 polynucleotide kinase (Takara) and [γ-32P]ATP ...
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bioRxiv - Genetics 2020Quote: ... a stepwise RNA end repair was carried out using T4 polynucleotide kinase (TaKaRa). A 3′-dephosphorylation and 5′-phosphorylation reaction was performed using T4 PNK enzyme (TaKaRa) ...
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bioRxiv - Plant Biology 2020Quote: ... YFP-CESA6 protein was detected using anti-GFP antibody (Takara, catalog # 632381) and SEC12 was detected using anti-SEC12 antibody (Bar-Peled and Raikhel ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech ...
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bioRxiv - Biochemistry 2021Quote: ... T4 polynucleotide kinase and TransIT®-LT1 Reagent were purchased from TAKARA (Shiga, Japan). SB203580 was purchased from Adipogen Life Science (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... X-alpha-Gal (X-α-gal) and aureobasidin A (AbA) were obtained from TaKaRa. Plasmids ...
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bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
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bioRxiv - Neuroscience 2024Quote: ... Confluent HEK293 cells were transfected using a mixture of 50 μl of Opti-MEM I containing a total of 0.9 μg of cDNA constructs encoding hGluN1/hGluN2 subunits and GFP (for identification of successfully transfected cells; pQBI 25, Takara) in a 1:1:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Neuroscience 2024Quote: ... was amplified by PCR and inserted into the Cre-dependent AAV hSyn FLEx vector using BamHI/KpnI restriction sites.pTet-on (transactivator) was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... corresponding to these sgRNA sequences (LpAsp_For and LpAsp_Rev as well as LpCht_For and LpCht_Rev) were phosphorylated by T4 polynucleotide kinase (TAKARA) followed by annealing and cloning into BbsI digested pSPneogRNAH vector (Zhang and Matlashewski ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Biochemistry 2024Quote: N-terminally HA-tagged IPMK (WT or the kinase deficient D144A mutant63) were cloned into pLPCX (Clontech). VSVg pseudotyped MLV was prepared by transfecting pLPCX-HA-IPMK along with pMLV-Gag-Pol and pVSVg into HEK293T cells using PolyJet transfection reagent (SignaGen) ...
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bioRxiv - Molecular Biology 2024Quote: ... EYFP-Mito (EYFP fused to mitochondrial targeting sequence of cytochrome C oxidase subunit VIII) and EYFP-ER (EYFP fused to Calreticulin ER-targeting sequence) were from Clontech (Takara Bio, Kusatsu, Japan); Vector for CRISPR/Cas9-mediated knock-out of BCL-2 was plentiCTRSPRv2 (Addgene #52961 ...
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bioRxiv - Microbiology 2020Quote: The VZV thymidine kinase gene (ORF36) was inserted into the Lenti-X pLVX-Puro vector (Clontech, Mountain View, CA) with XhoI and EcoR I sites ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
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bioRxiv - Microbiology 2023Quote: ... inverse PCR amplification was conducted with oligonucleotides and templates listed in Table S1 and the resulting PCR products were treated with T4 Polynucleotide Kinase (Takara) and ligated with a DNA ligation kit (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... synthesized 5’ and 3’ fragments of the target RNA were 32P-radiolabeled at the 5’ end using T4 polynucleotide kinase (Takara). After gel purification ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary oligonucleotides (0.1 nmole each) corresponding to the sgRNA sequences (LpBBS1gRNA3’UTR48F and LpBBS1gRNA3’UTR48R) were phosphorylated by T4 polynucleotide kinase (TAKARA), annealed ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then stained using primary antibodies against the reporter proteins mCherry (Clontech 632543 RRID: AB_2307319, 1:250) and against the panneuronal marker NeuN (millipore MAB377 RRID ...
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bioRxiv - Plant Biology 2020Quote: ... GFP-TSN-interacting proteins and native TSN were detected by mouse α -GFP (monoclonal antibody JL-8; Clontech) and rabbit α-TSN antibodies at final dilutions of 1:1,000 and 1:5,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The expression of Dunk protein was confirmed by Western blot using c-Myc Monoclonal Antibody (Takara, Cat# 631206). Then ...
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bioRxiv - Plant Biology 2021Quote: ... NPR1-GFP proteins were detected by reacting the blots with the mouse monoclonal anti-GFP monoclonal antibody (Clontech, USA) and horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...