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Citations for GE Life Sciences :
1 - 50 of 4645 citations for cAMP dependent protein kinase catalytic subunit alpha PRKACA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Biochemistry 2020Quote: Purified wt-Nsp15 and catalytic-deficient Nsp15 H235A were resolved over a Superdex-200 (GE Healthcare) gel filtration column using SEC Buffer to isolate the hexamer ...
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bioRxiv - Biophysics 2020Quote: ... a cAMP to which the fluorescent dye Cy3B (GE Healthcare, Frankfurt, Germany) was linked via an aminohexylthio spacer to position 8 of the adenosine moiety (64 ...
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bioRxiv - Cell Biology 2020Quote: NRVMs at a confluence of 50% were transfected with either control non-targeting siRNA or pooled siRNA targeting both AMPKα1 and AMPKα2 catalytic isoforms (AMPKα1/α2 siRNA) (from GE Healthcare 50nM) using lipofectamine RNAimax transfection reagent (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... without Alpha-Thioglycerol (GE Healthcare Life Sciences Cat. # SH30228.01); 1X GlutaMAX-I ...
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bioRxiv - Biophysics 2023Quote: ... cells were starved for cAMP competency on 27 mm nitrocellular lose filter pads (GE HealthCare) using the protocol described in [21].
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... and the σA subunit were expressed and purified by chromatography on HisTrap HP (GE Healthcare) Ni2+-affinity columns as described (Hu et al. ...
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bioRxiv - Immunology 2021Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare) as described previously (Sok et al. ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare). Concentrations of purified mAbs was determined using a NanoDrop One (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Genetics 2022Quote: ... Antibody–protein interactions were revealed using chemiluminesence (RPN2108, GE Healthcare), and relative protein expression was quantified using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescently labelled subunits were assessed by visualizing an SDS-PAGE gel with Typhoon FLA 9500 (GE Healthcare) with LPG filter and 100 μm pixel size ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fully formed complexes and disassembled subunits were separated on a Superose 6 10/300 column (GE Healthcare) equilibrated in SEC buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Detection of radiolabelled mt-DNA subunits was performed using an Amersham™ Typhoon™ Biomolecular Imager (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Plasma antibodies were purified using Protein A Sepharose resin (GE Healthcare), eluted from the resin with 0.1 M glycine at pH 2.5 and buffer exchanged into 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... The undigested antibodies were removed with Protein A Sepharose (GE Healthcare) and the flow through was collected ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified via HiTrap Protein A HP columns (GE Healthcare) run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare) ...
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bioRxiv - Genetics 2020Quote: ... using native Msh5 antibody and Protein A Sepharose beads (GE Healthcare). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The antibody was purified by protein G affinity column chromatography (GE Healthcare) (Supplementary Fig ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from culture supernatant with Protein G Sepharose (GE Healthcare). One mg anti-IL-4Rα or an isotype control (rat IgG2a anti-trinitrophenol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were revealed using the appropriate HRP-conjugated secondary antibodies (GE healthcare). Proteins were then identified using Immobilon Western (Millipore ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-chromatin complexes were immobilized on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2000 RU ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified by Protein A affinity chromatography column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-treated samples were next incubated with Protein A-Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... PON1 monoclonal antibody was coupled to protein G-Sepharose 4B beads (GE Healthcare) and eluted as described previously[69] ...
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bioRxiv - Immunology 2022Quote: ... Protein bands bound by antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific) ...