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No products found
because this supplier's products are not listed.
Hanyu Pan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Yang.17 The PD-1 blocking scFv E27 gene was synthesized by GENEWIZ (GENEWIZ, Suzhou, China) according to the previous report.36 3BNC117 CAR and E27 were cloned into a lentiviral vector (pTRPE-GFP-T2A-mRFP ...
-
No products found
because this supplier's products are not listed.
Anne-Sophie Hafner, et al.,
bioRxiv - Neuroscience 2019
Quote:
... After 30 min in blocking buffer cells were incubated with mouse anti-puromycin (Kerafast, 1:2000) for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Ji-Hoon Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... incubated with primary antibody against spike protein diluted 1:5,000 in blocking buffer (Elabscience, E-AB-V1006) overnight in a 37°C incubator with 5% CO2 injection ...
-
No products found
because this supplier's products are not listed.
Hengqian Ren, et al.,
bioRxiv - Biochemistry 2023
Quote:
... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...
-
No products found
because this supplier's products are not listed.
Nikolas Nikolaou, et al.,
bioRxiv - Neuroscience 2020
Quote:
... This was removed and coverslips were incubated with fresh blocking solution containing anti-GFP 1:1000 (Torrey Pines Biolabs-TP401) and anti-SNRNP70 1:200 (Sigma-AV40276 ...
-
No products found
because this supplier's products are not listed.
Rachel J. Harding, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HTT protein was eluted with 1 cell paste volume of buffer supplemented with 250 μg/mL 3xFLAG peptide (Chempep) run twice over the anti-FLAG resin ...
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No products found
because this supplier's products are not listed.
Chamseddine Ben Brahim, et al.,
bioRxiv - Cell Biology 2020
Quote:
... arrays were incubated 30 min Super G blocking buffer (Grace Biolabs), rinsed in water ...
-
No products found
because this supplier's products are not listed.
Natalie S. Haddad, et al.,
bioRxiv - Immunology 2020
Quote:
... Coupled microspheres were washed four times in a blocking buffer consisting of 1% BSA (Bovine Serum Albumin; Boston Bioproducts; Ashland, MA, USA), 1X PBS (VWR ...
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No products found
because this supplier's products are not listed.
Francesca Boscolo Sesillo, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Slides stained for collagen I were first washed and then incubated in in blocking buffer (10% goat serum, 0.3% Triton x-100, 1% BSA (Gemini Bio-Products, 700-100P) in PBS ...
-
No products found
because this supplier's products are not listed.
Wentian Luo, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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No products found
because this supplier's products are not listed.
Gemma L. Pearson, et al.,
bioRxiv - Cell Biology 2022
Quote:
... blocked for 1 h at room temperature with 1 X Blocking One solution (Nacalai USA Inc; San Diego, CA, USA). Sections were then incubated in the following primary antisera overnight at 4°C in 1 X PBS + 1% tween + 20% Blocking One solution ...
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No products found
because this supplier's products are not listed.
Sherif Salah, et al.,
bioRxiv - Immunology 2022
Quote:
... and membrane protein peptide (by AMSBIO) were procured ...
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No products found
because this supplier's products are not listed.
Simona Giunta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Incubations with primary antibodies were conducted in blocking buffer for 1 h at room temperature using the following antibodies: ACA (1:500; 15-235-0001, Antibodies Incorporated), ATR (1:1000 ...
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No products found
because this supplier's products are not listed.
Carly V. Weiss, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... dissociated with Trypsin/EDTA (Cell Applications Inc.), and plated at approximately 5,000 cells/cm2 ...
-
No products found
because this supplier's products are not listed.
Francisco Santos, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and blocking with 5% goat serum (Abbkine Scientific) and 0.3% Triton X-100 in PBS1x for 1h at RT with agitation ...
-
No products found
because this supplier's products are not listed.
D Muñoz-Reyes, et al.,
bioRxiv - Biochemistry 2022
Quote:
... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
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No products found
because this supplier's products are not listed.
Antonio Dominguez-Meijide, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... a blocking step with 1.5% BSA (NZYTech, Lisbon, Portugal) was performed for 1 hour ...
-
No products found
because this supplier's products are not listed.
Sara R Roig, et al.,
bioRxiv - Cell Biology 2021
Quote:
The following peptides were purchased from EMC microcollections GmbH ...
-
No products found
because this supplier's products are not listed.
Mireia Rovira, et al.,
bioRxiv - Immunology 2022
Quote:
... Peptides were purified using SDB-RPS columns (Affinisep). Briefly ...
-
No products found
because this supplier's products are not listed.
Rick G. Kim, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Biotinylated peptides were searched using exogenous biotin (ExBio) on lysine as a variable modification ...
-
No products found
because this supplier's products are not listed.
Eric E. Gardner, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 0.05% Triton X-100 in 1X PBS pH 7.2 supplemented with mouse-on-mouse blocking reagent (Vector Biolabs; 1:50) and Fc receptor blocker reagent (Innovex Biosciences ...
-
No products found
because this supplier's products are not listed.
Jhansi L. Leslie, et al.,
bioRxiv - Microbiology 2019
Quote:
... each plate had a positive control consisting of toxin coated wells reacted with mouse TcdA monoclonal antibody TGC2 diluted 1:5,000 in blocking buffer (antibodies-online.com ABIN335169). The optical density at 410nm and 650nm was recorded on a VersaMax plate reader (Molecular Devices ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... antibodies blocking the type I IFN receptor (mouse anti-human IFNAR2 antibody, clone MMHAR-2, PBL Assay Science Cat#21385-1) or isotype control (Ultra-LEAF Purified mouse IgG2a ...
-
No products found
because this supplier's products are not listed.
Nikola Cousin, et al.,
bioRxiv - Immunology 2021
Quote:
OT-1 effector T cells were generated by ex vivo culture of total Ly5.1+ OT-1 splenocytes in T cell medium supplemented with 1 ng/ml SIINFEKL peptide and 100 U/ml recombinant mouse IL-2 (ImmunoTools) for 72 h ...
-
No products found
because this supplier's products are not listed.
Gregory A. Breuer, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Blocking was performed in TBS-T with 5% BSA (Gold Biotechnology) for 1h at room temperature ...
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No products found
Pooja Khanna, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Membranes were blocked with Advanblock blocking buffer (Advansta R-03726-E10) for 1 hour at room temperature and incubated at 4°C in primary antibody overnight ...
-
No products found
because this supplier's products are not listed.
Wei Ding, Walter A. Koltun, Gregory S. Yochum,
bioRxiv - Cell Biology 2024
Quote:
... The cover slips were then incubated with the following primary antibodies diluted in blocking/staining buffer overnight at 4°C: anti-MUC5B (1:200, Atlas Antibodies, HPA008246); anti-E-cadherin (1:200 ...
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No products found
because this supplier's products are not listed.
Sebastian Boland, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Immunostaining for 2 hours was performed with the following primary antibodies (1:100 dilution in blocking buffer): GM2 (Tokyo Chemical Industry Cat#2576), LAMP1 (Cell Signaling Technology Cat#9091) ...
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No products found
because this supplier's products are not listed.
Douek-Maba Orit, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... followed by an overnight incubation at 4°C in blocking solution with anti phospho-histone 3 (PhH3) antibody or anti-caspase 3 (Cas3) antibody (both 1:300; Lifespan Bioscience. Washington US). Next ...
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No products found
because this supplier's products are not listed.
Pragya Parashara, et al.,
bioRxiv - Cell Biology 2024
Quote:
... PLK1PBD was concentrated to 6 mg/ml and mixed with two times molar excess of S54ph peptide The mixture was incubated on ice for at least 1 h before setting up crystallisation trays with Morpheus® (Molecular Dimensions) screen using the ART Robbins Crystal Gryphon crystallisation robot in 96-well sitting drop MRC plates at 18ºC ...
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The CY3 (Red) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically...
Cat# 5276-60UG,
0.3 mg, USD $290.0
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Amber L. Altrieth, et al.,
bioRxiv - Cell Biology 2023
Quote:
Collagen hybridizing peptide (CHP) conjugated to 5-carboxyfluorescein (5-FAM) (Advanced BioMatrix) was solubilized per manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Ishraq Alshanqiti, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Michael Caterina at Johns Hopkins University) and guinea pig anti-calcitonin gene-related peptides (CGRP) (1:1,000; Peninsula Laboratories International, San Carlos, CA, USA)) ...
-
No products found
because this supplier's products are not listed.
Bijal A. Parikh, et al.,
bioRxiv - Immunology 2019
Quote:
... Fc receptor blocking was performed with 2.4G2 (anti-FcγRII/III) hybridoma (American Type Culture Collection) culture supernatants ...
-
No products found
because this supplier's products are not listed.
Marcin Pęziński, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and 100 nM Actistain conjugated to AlexaFluor 555 (in blocking buffer; catalog no. PHDH1, Cytoskeleton), followed by three washes with PBS ...
-
No products found
because this supplier's products are not listed.
Emilio Boada-Romero, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Experiments using anti-PS blocking antibody were performed in µ-Slide Angiogeneis chambers (IBIDI, 81506) and cell numbers were scaled down accordingly.
-
96 well glass bottom plate. Black polystyrene frame with #1 glass(0.13-0.16mm), with lid,...
Cat# P96-1-N,
20/case, $226.00
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William Bloxham, et al.,
bioRxiv - Biophysics 2021
Quote:
... cells were split using trypsin onto glass-bottom dishes (In Vitro Scientific, D35-20-1.5-N) coated with poly(D-lysine ...
-
The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues...
Cat# B23111, SKU# B23111-4mg,
4mg, $299.00
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Caillan Crowe-McAuliffe, et al.,
bioRxiv - Biochemistry 2020
Quote:
... RqcH-GS-FLAG3 was eluted by addition of 200 μL opening buffer containing 0.1 mg/mL poly-FLAG peptide (Biotool, Bimake) for 45 min on a turning wheel ...
-
No products found
because this supplier's products are not listed.
Natalie Schindler, et al.,
bioRxiv - Genetics 2022
Quote:
... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
-
No products found
because this supplier's products are not listed.
Matthew P. Ostrowski, et al.,
bioRxiv - Microbiology 2021
Quote:
... sequences were designed to remove N-terminal signaling peptides and to add a histidine tag for immobilized metal affinity chromatography (IMAC) (in many cases using the Lucigen MA101-Expresso-T7-Cloning-&-Expression-System) ...
-
No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
The linear optical properties of the GRAB peptide sensors expressed in HEK293T cells were measured using a Safire 2 plate reader (Tecan). Cells were harvested and transferred to black-wall 384-well plates containing either saline alone or saline containing the corresponding peptides ...
-
No products found
because this supplier's products are not listed.
Bibekananda Sahoo, et al.,
bioRxiv - Immunology 2023
Quote:
... The bound proteins were then reconstituted on-column into peptidisc22 or amphipol by incubation in 5ml of washing buffer supplemented with 10mg peptidisc peptide (Peptidisc Lab) or amphipol A8-35 (Anatrace) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Xue Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
-
No products found
because this supplier's products are not listed.
Yihong Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Culture medium containing 2 % supplements and different concentration of FOL-026 peptide was added and images recorded with an inverted microscope (Nikon Eclipse TE2000-U ...
-
No products found
because this supplier's products are not listed.
Jennifer E. Wu, et al.,
bioRxiv - Immunology 2023
Quote:
... P14 transgenic mice expressing a TCR specific for the LCMV peptide DbGP33-41 were bred in-house and backcrossed onto the C57BL/6 background (Charles River); recipient WT C57BL/6 mice were purchased from Charles River ...
-
No products found
because this supplier's products are not listed.
Yee Jher Chan, et al.,
bioRxiv - Bioengineering 2024
Quote:
... the cells were removed with trypsin and the height profile of the matrix was analyzed using a digital microscope (Olympus DSX 110).
-
No products found
because this supplier's products are not listed.
Fenghui Zhao, et al.,
bioRxiv - Molecular Biology 2021
Quote:
The purified peptide 20–GLP-1R–Gs–Nb35 complex (3.5 μL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Duy Lan Huong Bui, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
-
No products found
because this supplier's products are not listed.
Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
-
No products found
because this supplier's products are not listed.
Safiye Aktas, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Math-1 (Bioss), and calretinin (Bioss ...
-
No products found
because this supplier's products are not listed.
Sodai Yoshimura, et al.,
bioRxiv - Neuroscience 2024
Quote:
... with an 1× objective (EC Plan-Neofluar 1×/0.025 M27, Carl Zeiss, Jena, Germany). The infarct area was quantified using the AxioVision Rel 4.8 software (Carl Zeiss ...