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No products found
because this supplier's products are not listed.
Yosuke Ito, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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No products found
because this supplier's products are not listed.
Kirsi Savijoki, et al.,
bioRxiv - Microbiology 2019
Quote:
Trypsin-digested peptides were purified using ZipTips (C18) (Millipore), and equal amounts of the peptide samples were subjected to LC-MS/MS using an Ultimate 3000 nano-LC (Dionex ...
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No products found
because this supplier's products are not listed.
Bertrand Fabre, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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No products found
because this supplier's products are not listed.
Tingxu Chen, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... in blocking buffer (1% blocking reagent [Roche] ...
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No products found
because this supplier's products are not listed.
Candace M. Cham, et al.,
bioRxiv - Pathology 2022
Quote:
... Jagged-1 peptide (1 uM, Genscript), Y-27632 (10 uM ...
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No products found
because this supplier's products are not listed.
Richard J Mills, et al.,
bioRxiv - Cell Biology 2021
Quote:
... After 1 h blocking using a 1:1 mix of LI-COR Odyssey Blocking Buffer (LI-COR Biotechnology) and PBS ...
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No products found
because this supplier's products are not listed.
Samuel Rivero-Hinojosa, et al.,
bioRxiv - Cancer Biology 2021
Quote:
HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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Supplied as dialyzed and lyophilized powder.
Cat# LS003702,
100 mg, $28.00
Ask
Jessie J.-Y. Chang, et al.,
bioRxiv - Immunology 2023
Quote:
... supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington), penicillin (100 IU/mL) ...
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No products found
because this supplier's products are not listed.
Daria Amiad Pavlov, et al.,
bioRxiv - Cell Biology 2024
Quote:
... cells were placed in blocking buffer (1:1 Seablock (Abcam) and 0.1% TritonX-100 (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Ameen A. Salahudeen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1 μg/ml JAG1 peptide (Anaspec), 500 nM soluble recombinant NOTCH receptor inhibitor Delta Like Ligand 4 mutant (DLL4E12) ...
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No products found
because this supplier's products are not listed.
Julia D. Berkson, et al.,
bioRxiv - Immunology 2023
Quote:
... 200 μL of blocking buffer (PBST wash buffer, 1% Biorad Blocking Agent) was then added and incubated overnight at 4°C ...
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No products found
because this supplier's products are not listed.
David Kabelik, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and pS6 blocking peptide (1220S, Cell Signaling Technology) antibody eliminated signal (Supplementary Fig ...
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No products found
because this supplier's products are not listed.
Arinze E. Okafor, et al.,
bioRxiv - Cell Biology 2022
Quote:
... blocking reagent (Vector Laboratories, MKB-2213-1) according to the instruction ...
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No products found
because this supplier's products are not listed.
Timothy Recaldin, et al.,
bioRxiv - Immunology 2023
Quote:
... blocking antibodies to TNFa (MAb-1, Biolegend) were added at a concentration of 1 ug/mL ...
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No products found
because this supplier's products are not listed.
Minyong Chen, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and tryptic peptides were generated using Trypsin-Ultra (P8101S, NEB) following the protocol described previously2 ...
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No products found
because this supplier's products are not listed.
Axelle Wilmerding, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 1% Blocking Reagent (BR, Merck 11096176) and 10% goat serum ...
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No products found
because this supplier's products are not listed.
Benjamin J. Meckiff, et al.,
bioRxiv - Immunology 2020
Quote:
... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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No products found
because this supplier's products are not listed.
Koya Yoshizawa, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were co-treated with 10 µM MG132 (Peptide Institute; for blocking anaphase onset) and 100 nM SiR-DNA (Cytoskeleton inc. ...
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No products found
because this supplier's products are not listed.
Endika Prieto-Fernández, et al.,
bioRxiv - Cell Biology 2021
Quote:
... washed with blocking buffer and incubated with fluorochrome-conjugated antibodies (1:100 dilution in blocking buffer) against syndecan-1 (BD Biosciences Cat#: 565943), syndecan-2 (R&D Systems Cat# ...
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No products found
because this supplier's products are not listed.
Anatoly Urisman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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No products found
because this supplier's products are not listed.
Riki Kawamura, Masato Nikaido,
bioRxiv - Neuroscience 2022
Quote:
... and treated with 1% blocking reagent (PerkinElmer) in TBS (Tris-buffered saline ...
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No products found
because this supplier's products are not listed.
Theresa Schmid, et al.,
bioRxiv - Immunology 2023
Quote:
Peptides were generated by custom peptide synthesis (Biomatik) or provided as peptide pools (JPT or Miltenyi BioTec ...
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No products found
because this supplier's products are not listed.
Pravin B. Sehgal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Blocking peptides corresponding to human STAT2 and human giantin (1-469) were purchased from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Nicholas S. Wilcox, et al.,
bioRxiv - Cell Biology 2021
Quote:
... After 1 h blocking with the blocking buffer (Rockland), we probed the membranes sequentially with mouse anti-HuR (clone 3A2 ...
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No products found
because this supplier's products are not listed.
Ryoji Amamoto, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... diluted in Blocking Buffer (1:750, Jackson ImmunoResearch) for 1 hour at RT ...
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No products found
because this supplier's products are not listed.
Inigo San-Millan, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Trypsin (0.25% trypsin, 25-053-CI, Corning) was utilized during culture expansion.
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No products found
because this supplier's products are not listed.
Lukasz Chrobok, et al.,
bioRxiv - Neuroscience 2021
Quote:
... glucagon-like peptide 1 (GLP-1; 1µM, Bachem), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX ...
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No products found
because this supplier's products are not listed.
John E. La Marca, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... dissociation with 1× trypsin (Lonza #BE02-007E), washing again with TC-PBS ...
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No products found
because this supplier's products are not listed.
Corey Tan, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-CD40L blocking Ab (clone MR-1, BioXcell).
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No products found
because this supplier's products are not listed.
M. Martin VandenBroek, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Blocking and incubations with Caprin-1 primary (Proteintech, 15112-1-AP, 1:500 dilution) and Alexa488 conjugated secondary antibodies were performed with buffers containing RNaseOUT RNase inhibitor (both ThermoFisher) ...
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No products found
because this supplier's products are not listed.
Joshua Johnson, et al.,
bioRxiv - Physiology 2020
Quote:
... PD-1 blocking antibody (R&D Systems/Techne, #AF1086) was included at the 10 µg/ml ...
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No products found
because this supplier's products are not listed.
Kirsten L. Viola, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Aβ1-42 (American Peptide or Peptides International) or FAM-Aβ1-42 (Anaspec ...
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No products found
because this supplier's products are not listed.
Hsin-I Huang, et al.,
bioRxiv - Immunology 2021
Quote:
... these were co-cultured 1:1 in complete RPMI containing OVA peptide (5 mg/mL, InvivoGen) and recombinant TGFβ (1 ng/mL ...
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No products found
because this supplier's products are not listed.
Shan Huang, et al.,
bioRxiv - Pathology 2019
Quote:
... After blocking with 1% bovine serum albumin (BSA) (VWR, Radnor, PA, US), the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA ...
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No products found
because this supplier's products are not listed.
Dheva Setiaputra, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and 1 µM of Shield-1 peptide (Clontech, Mountain View CA) for 4-6 hours ...
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No products found
because this supplier's products are not listed.
Jose Paulo Lorenzo, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Trypsin-digested peptides in the supernatant were dried in a SpeedVac (Eppendorf) for 3h at 30 °C ...
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No products found
because this supplier's products are not listed.
Ranjie Xu, et al.,
bioRxiv - Neuroscience 2019
Quote:
... PSD95 antibody (2.5 μg/ml) was incubated with five times PSD95 blocking peptide (12.5 μg/ml, Synaptic System) or equivalent amount of PBS at RT for 30 minutes before applying secondary antibodies to the brain tissues as suggested by the manufacturer instructions.
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No products found
because this supplier's products are not listed.
Michelle Ly, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Embryos were injected with 1 mM RGDS peptide (Tocris Bioscience) in water ...
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No products found
because this supplier's products are not listed.
Damon A. Hofman, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or 1 μg/μl 3x FLAG peptide (ApexBio) at 37 °C for 15 min with shaking at 1,000 RPM The two elution fractions were pooled and samples were prepared with 4x LDS sample buffer and 10× sample-reducing agent (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Kwadwo A. Opoku-Nsiah, et al.,
bioRxiv - Biochemistry 2021
Quote:
Peptides were synthesized by Fmoc solid phase peptide synthesis on a Syro II peptide synthesizer (Biotage) at ambient temperature and atmosphere on a 12.5 μM using either pre-loaded Wang resin or Rink amide resin (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
... 100 µM TIRAP (TLR2 and TLR4) inhibitor peptide or control peptide (Novus Biologicals) were added to neutrophils at 1 h before incubation.
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No products found
because this supplier's products are not listed.
Katarzyna B. Leszczynska, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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No products found
because this supplier's products are not listed.
Qing Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the blocking buffer (1x Fish Gelatin Blocking Agent (22010, BioTium), 0.5% Triton x-100 in PBS ...
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No products found
because this supplier's products are not listed.
Rémi Porte, et al.,
bioRxiv - Immunology 2022
Quote:
... then 100 ng/ml of synthetic competence-stimulating peptide 1 (CSP-1; Eurogentec) was added for 12min at 37°C to activate transformation machinery ...
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No products found
because this supplier's products are not listed.
Zhipeng Li, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and incubated in blocking solution (1% BSA, 0.3M glycine in DPBS) for 30 min ...
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No products found
because this supplier's products are not listed.
Yan Hu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... followed by an enzymatically digested in 1% trypsin (MP Biomedicals, 153571) for 1-2 hours at 37 °C ...
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No products found
because this supplier's products are not listed.
Manjunatha Chandana, et al.,
bioRxiv - Microbiology 2021
Quote:
... In-solution trypsin (SCIEX) digestion was carried out for 200 μg total protein ...
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No products found
because this supplier's products are not listed.
Ran Yan, Douglas T. Fearon,
bioRxiv - Immunology 2022
Quote:
... followed by blocking with 1% BSA (Calbiochem, 2930) and 10% normal goat serum (Thermo ...
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No products found
because this supplier's products are not listed.
Cecilia Perez-Borrajero, et al.,
bioRxiv - Biochemistry 2021
Quote:
The lyophilized TOST-1 and PID-1 peptides were purchased from Proteogenix at 95 % purity ...
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No products found
because this supplier's products are not listed.
Qinghuang Lin, et al.,
bioRxiv - Zoology 2024
Quote:
The trypsin-digested peptides are dried by vacuum freeze-drying with Strata X C18 (Phenomenex). Dissolve the peptides in 0.5 M TEAB and thaw the labeling reagent ...