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No products found
because this supplier's products are not listed.
Coral K. Wille, et al.,
bioRxiv - Genetics 2023
Quote:
... 1x PBS) and probed for 1 hour in blocking buffer containing α-NANOG (1:100; Cosmo Bio USA, REC-RCAB001P or 1:1000 ...
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No products found
because this supplier's products are not listed.
Julie Zaworski, et al.,
bioRxiv - Genetics 2020
Quote:
... 1 mM MgCl2) was digested with trypsin using a FASP protocol (Filter Aided Sample Prep from Expedeon) (Wisniewski ...
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No products found
because this supplier's products are not listed.
James B. Bower, Scott A. Robson, Joshua J. Ziarek,
bioRxiv - Biophysics 2024
Quote:
... The dissolved peptide was added to a 1 mL Float-A-Lyzer G2 Dialysis Device from Repligen with a 0.5-1 kDa cutoff ...
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No products found
because this supplier's products are not listed.
Ni-Chen Chang, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... After blocking with 10% Donkey Serum (Equitech-Bio, sd300500) in TBTD for 1 hour ...
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No products found
because this supplier's products are not listed.
Andrew T. Phillips, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Wells were then washed 4 times with TBST and then incubated in 1x Blocking Buffer in PBS with a 1:10,000 dilutions of either β1- (Assay Biotechnology; San Francisco ...
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No products found
because this supplier's products are not listed.
Frank Hidalgo, et al.,
bioRxiv - Biophysics 2022
Quote:
... Peptides were desalted for 4 minutes on a trap column (1 mM ID x 2 cm, IDEX C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific 1112906) ...
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No products found
because this supplier's products are not listed.
Chad R. Simmons, et al.,
bioRxiv - Bioengineering 2023
Quote:
... prepared in the laboratory using an adaption of a previously described protocol.58 Peptide synthesis was performed in a peptide synthesis reaction vessel (ChemGlass, CG-1860) with each reaction step carried out on a Burrel Scientific wrist action shaker ...
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No products found
because this supplier's products are not listed.
Francisco J. Cao-Garcia, et al.,
bioRxiv - Biophysics 2023
Quote:
... After HaloTag ligand functionalization the fluid chambers were passivated for at least 3 h with blocking buffer containing 1% w/v sulfydryl-blocked BSA (Lee Biosolutions) in 20 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Adeline M. Fanni, et al.,
bioRxiv - Neuroscience 2021
Quote:
... the peptide solution was loaded into a quartz cuvette with a path length of 1 mm (Starna cells Inc, Atascadero, CA) and analyzed on an AVIV 410 CD Spectrometer (AVIV ...
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No products found
because this supplier's products are not listed.
Samir M. Abdelmagid, et al.,
bioRxiv - Cell Biology 2020
Quote:
The MicroVue™ Helical Peptide EIA kit (Quidel, San Diego, CA) was used to measure the level of a helical peptide containing the residues 620-633 of the type I collagen α1 chain or more formally carboxy-terminal collagen crosslinks ...
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No products found
because this supplier's products are not listed.
Francesco Caiazza, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and peptides desalted with C18 Desalting Tips (Rainin, Oakland, CA, USA), lyophilized ...
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No products found
because this supplier's products are not listed.
Hem Gurung, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The peptide-buffer mixtures were dispensed and reformatted into 384 well plates (Labcyte) at a volume of 47.5 μl per well ...
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No products found
because this supplier's products are not listed.
Tyson J. Ruetz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10] ...
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No products found
because this supplier's products are not listed.
Chanchan Xiao, et al.,
bioRxiv - Immunology 2021
Quote:
... 0.5×106 CD8+ T cells isolated from health donors were co-cultured with 0.5 × 106 peptide-loaded T2A2 cells stained with 5 µmol/L CFSE (TargetMol), and stimulated with 1 µg/mL anti-human CD28 antibodies (BioLegend ...
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No products found
because this supplier's products are not listed.
Barr Tivon, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 4 µl of 20 mM alkyne-peptide in DMSO were mixed with 10 µl of 5 mM BDP-TMR-azide (Lumiprobe). To the mixture was added 50 µl water ...
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No products found
because this supplier's products are not listed.
Sandeep Adem, et al.,
bioRxiv - Bioengineering 2020
Quote:
Biotin-PEG36-Thr-Phe-Ser-Tyr-Nle-Arg-Trp-Pro-PEG12-Cys (known as peptide) was synthesized by CPC Scientific and Biotin-PEG-SH (known as linker) was purchased from NANOCS. Peptide (2 µM ...
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No products found
because this supplier's products are not listed.
Takafumi Kato, et al.,
bioRxiv - Pathology 2021
Quote:
... PRR4 cDNA without a signal peptide sequence (corresponding to amino acids 17 to 134) was cloned into the pM-secSUMOstar Vector (7121, LifeSensors). SUMOstar-PRR4 vectors were transfected into Expi293 cells (1 mg of DNA per liter of transfection ...
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No products found
because this supplier's products are not listed.
Alexander T. Hilditch, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
Solid-phase peptide synthesis (SPPS) reagents were purchased from Cambridge Reagents with the exception of N,N’-diisopropylcarbodiimide (DIC) purchased from Carbosynth. Rink amide MBHA resin and Fmoc-protected amino were purchased from Merck ...
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No products found
because this supplier's products are not listed.
Erin M. Euliano, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Near-infrared fluorescent peptides were made by coupling 2 equivalents N-hydroxysuccinimide (NHS) ester-functionalized ATTO 647N (ATTO-TEC, Siegen, Germany) to the N-terminus with 4 equivalents of DIEA overnight protected from light ...
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No products found
because this supplier's products are not listed.
Jonathan M Budzik, et al.,
bioRxiv - Microbiology 2019
Quote:
Immunostaining for LC3 was performed with blocking and permeabilization buffer (0.3% Triton X-100, 2% bovine serum albumin) and anti-LC3 (clone 2G6, Nanotools #0260-100/LC3-2G6) at a dilution of 1:200 for 3 hours at room temperature ...
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No products found
because this supplier's products are not listed.
Shivani Sachdev, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
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No products found
because this supplier's products are not listed.
Mateo F. Espinoza, et al.,
bioRxiv - Cell Biology 2022
Quote:
... After each injection the MuDPIT column was washed with buffer A and then the peptides separated over a 25 cm analytical column (100 μm ID, 5 μm tip pulled on a Sutter Instruments T-2000 tip puller) packed with 3 μm C18 resin (Phenomenex ...
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No products found
because this supplier's products are not listed.
Khomgrit Morarach, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1% BSA and 1% DRAQ7 (Biostatus). Tomato+ cells were sorted by flow cytometry on a BD Influx™ cell sorter and collected in ice-old PBS containing 0.04% BSA.
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No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... PDE11A (FabGennix PD11-112 at 1:100; Aves custom PDE11A4 #1 at 1:10,000; Fabgennix PPD11A-140AP at 1:1000; Fabgennix PPD11A-150AP at 1:500), NF-L (Cell Signaling #2837 at 1:10,000) ...
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No products found
because this supplier's products are not listed.
Lisa Maria Metz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... GPVI (JAQ1-FITC, #M011-1, Emfret Analytics, 1:10), integrin α5 (CD49e ...
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No products found
because this supplier's products are not listed.
Marie E Jönsson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 mM DTT) using a 1 ml tissue douncer (Wheaton). The homogenate was carefully layered on top of a sucrose cushion (1.8 M sucrose ...
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No products found
because this supplier's products are not listed.
Laurent Jacob, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Rabbit anti–mouse LYVE-1 (11-034, AngioBio, 1:800), Goat anti– human PROX1 (AF2727 ...
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No products found
because this supplier's products are not listed.
Wensheng Chen, Darrell Pilling, Richard H. Gomer,
bioRxiv - Immunology 2022
Quote:
... or 1 or 10 μM IN-1 (AdooQ, Irvine, CA) from a 10 mM stock in DMSO (VWR ...
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No products found
because this supplier's products are not listed.
Nahima Saliba, et al.,
bioRxiv - Biophysics 2023
Quote:
... Tubing (FEP 1/16” OD, 1/100” ID, Cole-Parmer) was connected from the pump to the microfluidic chip for solution perfusion.
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No products found
because this supplier's products are not listed.
Marijke I. Zonneveld, et al.,
bioRxiv - Immunology 2020
Quote:
... and 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 Sanquin) in the presence or absence of milk EVs or EV depleted control ...
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No products found
because this supplier's products are not listed.
Jonas L. Ravn, et al.,
bioRxiv - Microbiology 2022
Quote:
... were inoculated separately with a starting OD600= 0.1 or in different ratios (1:1 and 1:10) in Delft medium + 2% beechwood glucuronoxylan (Megazyme, Ireland) for 120 h at RT ...
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No products found
because this supplier's products are not listed.
Salla Kyheröinen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Phospho-Ser3 cofilin antibody (11139-1, 1:1000) was from Signalway antibody. The following secondary antibodies were from Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Mari Takalo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... IP-1 ELISA (Cisbio) assay was then used according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Julia Böhme, et al.,
bioRxiv - Immunology 2020
Quote:
The activity of caspase-1 was determined using fluorescent inhibitor of caspase-1 (FAM-Flica Caspase-1 Assay kit, ImmunoChemistry Technologies). Staining procedure was performed according to manufactures protocol ...
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No products found
because this supplier's products are not listed.
Hartwig Seitter, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 µM TTX (Biotrend, BN0518), pH = 7.4 with NaOH (S2770) ...
-
No products found
because this supplier's products are not listed.
Hualin Fu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Hemin (Absolute Antibody, 1D3, 1:100). The following secondary antibodies and dilutions have been used in this study ...
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No products found
because this supplier's products are not listed.
Wenhao Xu, et al.,
bioRxiv - Microbiology 2023
Quote:
... 1-μl capillaries (Microcaps, Drummond Scientific) were heat-sealed at one end and filled with buffer (control ...
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No products found
because this supplier's products are not listed.
Cheng Wu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Microglass pipettes filled with 1 μl Aβ-555 peptide solution were connected to a microinjection system (BASi, USA). Aβ40-555 were used among hypertension groups and Aβ42 555 were used among APP/PS1 groups ...
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No products found
because this supplier's products are not listed.
Tommaso Montecchi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... SEB and SEE peptides (2μg/ml) (Toxin Technology) or 1% BSA for controls ...
-
No products found
because this supplier's products are not listed.
Linda Balabanian, et al.,
bioRxiv - Biophysics 2021
Quote:
... Cells were then washed with Blocking Buffer: 2% (w/v) BSA (BioShop Canada Inc., Burlington, ON), 0.2% (w/v ...
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No products found
because this supplier's products are not listed.
Anuj kumar Murmu, et al.,
bioRxiv - Genomics 2022
Quote:
The predicted peptide sequence of Mucin 2 of indigenous duck was derived by Edit sequence (Lasergene Software, DNASTAR) and then aligned with the peptide of other chicken breed and avian species using Megalign sequence Programme of Lasergene Software ...
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No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated flagellin peptides were conjugated on to Streptavidin coated 4um polystyrene beads (Spherotech, Inc. Cat # PAK-4067-8K). Beads conjugated with each individual peptide have different fluorescent intensity in the APC/Cy7 channel so that they can be differentiated from each other by flow cytometry ...
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No products found
because this supplier's products are not listed.
Jonathan R. Soucy, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The collected spinal cords were minced into ~2 mm pieces and transferred to a 0.2% (v/v) trypsin solution in Hibernate®-A without Ca2+ (BrainBits, LLC) at 37 °C to dissolve the neural tissue ...
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Cat# AG044-1,
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Hanieh Ghassabian, et al.,
bioRxiv - Microbiology 2020
Quote:
... α-UL44 mAb (Virusys Corporation, #P1202-1; 1:100); α-pp65 mAb (Virusys Corporation ...
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No products found
because this supplier's products are not listed.
Magdalena Ziółkowska, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Squares of approximately 1 × 1 × 1 mm were attached to aluminium pins (Gatan metal rivets, Oxford instruments) with very little amount of cyanacrylamide glue ...
-
No products found
because this supplier's products are not listed.
Hua He, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... or NKX2-1 antibody (Seven Hills Bioreagents, WRAB-1231, 1:1000) followed by incubation with TrueBlot HRP-Conjugated secondary antibody (Rockland Immunochemicals Inc ...
-
No products found
because this supplier's products are not listed.
Dimitre R. Simeonov, et al.,
bioRxiv - Immunology 2020
Quote:
... 1 photomultiplier tube (PMT), 40x (NA = 1.3 ...
-
No products found
Mercedes Lachén-Montes, et al.,
bioRxiv - Systems Biology 2022
Quote:
... and 1% penicillin/streptomycin (ABM) and grown in a 5% CO2 humidified atmosphere at 37°C ...
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No products found
because this supplier's products are not listed.
Megan Mey, et al.,
bioRxiv - Neuroscience 2023
Quote:
... TrkB ser478 (1:1,000; Biosensis, CA), LHCGR (1:500 ...
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No products found
because this supplier's products are not listed.
Mário Hüttener, et al.,
bioRxiv - Microbiology 2021
Quote:
... 10 g l-1 tryptone and 5 g l-1 yeast extract) with vigorous shaking at 200 rpm (Innova 3100, New Brunswick Scientific). The antibiotics used were chloramphenicol (Cm ...