-
No products found
because this supplier's products are not listed.
Timothy A Fenton, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The SynGAP1 immunolabeling was performed using a commercially available Anti-SynGAP1 antibody (1:250; PA1-046; ThermoFisher Scientific), incubating overnight at 4°C with orbital agitation ...
-
No products found
because this supplier's products are not listed.
Allison Knupp, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The following primary antibodies were used: Ras-related protein Rab-5A (RAB5A) at 1:500 (Synaptic Systems 108 011); early endosome antigen 1 (EEA1 ...
-
No products found
because this supplier's products are not listed.
Ozgun Erdogan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... or Ras GTPase ELISA Kit (Abcam, ab134640) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Miles A. Keats, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
-
No products found
because this supplier's products are not listed.
Christian A. E. Westrip, et al.,
bioRxiv - Biochemistry 2023
Quote:
GTPase activity of the purified protein was measure using the Promega GTPase-Glo™ kit (Promega, V7681), as per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ali Vural, Stephen M. Lanier,
bioRxiv - Cell Biology 2023
Quote:
... Ras GTPase-activating protein-binding protein 1 (G3BP1) phage UbiC G3BP1-GFP-GFP was a gift from Jeffrey Chao (Addgene plasmid # 119950 ...
-
No products found
because this supplier's products are not listed.
Marcella Birtele, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... SYNGAP1 was immunoprecipitated using 2ug/ml of SYNGAP1 antibody (Cell Signaling 5540) and 3mg/ml of total protein lysate ...
-
No products found
because this supplier's products are not listed.
Swati Mishra, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Ras-related protein Rab-11 (Rab11) at 1:250 (#610656; BD Biosciences); Lysosome associated membrane protein-1 (LAMP1 ...
-
No products found
because this supplier's products are not listed.
Sergio Espinosa-Gil, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and anti-Fas activating antibody (Merck) was diluted in 50% glycerol in PBS.
-
No products found
because this supplier's products are not listed.
Moon Hee Yang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and immunoblotting was performed to detected GTP-bound Ras proteins using anti-K-Ras antibody (Proteintech, 12063-1-AP), anti-RasG12D mutant specific antibody (Cell Signaling Technologies [CST] ...
-
No products found
because this supplier's products are not listed.
Diego Alem, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Ras GTPase Chemi ELISA Kit (Active Motif, 52097) was used to measure Ras GTPase level in organoids ...
-
No products found
because this supplier's products are not listed.
Marc Creixell, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The AXL-activating antibody AF154 (R&D Systems) was used at 300 ng/mL ...
-
No products found
because this supplier's products are not listed.
Hanna van Ooijen, et al.,
bioRxiv - Immunology 2024
Quote:
Plasma-treated plastic chips were coated with antibodies against NK cell activating receptors LFA-1 (BioLegend, clone HI111) and CD16 (BioLegend ...
-
No products found
because this supplier's products are not listed.
Dan Sarni, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The primary antibodies used in these analyses were rabbit anti-H-RAS (Santa Cruz, 1:1,000), mouse anti- CHK1 (Cell signaling ...
-
No products found
because this supplier's products are not listed.
Yudong Gao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mutation of Syngap1 (SYNGAP1-c.2214_2217del) was introduced by site-directed mutagenesis (QuikChange Lightning, Agilent #210518). HEK293T cells were co-transfected with expression vectors using Lipofectamin 3000 (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Irune Guerra San Juan, et al.,
bioRxiv - Neuroscience 2024
Quote:
... retinoic Acid (RA, 1 µM, Tocris) and smoothened agonist (SAG ...
-
No products found
because this supplier's products are not listed.
Hari Krishnamurthy, et al.,
bioRxiv - Immunology 2022
Quote:
The RA panel included anti-RF IgM (Roche Diagnostics ...
-
No products found
because this supplier's products are not listed.
David Koschut, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
-
No products found
because this supplier's products are not listed.
Toshiya Kimura, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and detected with a CCD imager (RAS-4000; GE healthcare). The following primary antibodies were used ...
-
No products found
because this supplier's products are not listed.
Piotr Michaluk, Janosch Heller, Dmitri A. Rusakov,
bioRxiv - Neuroscience 2020
Quote:
Primary antibodies used: post-synaptic protein PSD-95 (mouse, 6G6-1C9, recombinant rat PSD-95, Novus Biologicals, NB110-61643 ...
-
No products found
because this supplier's products are not listed.
Malte S. Kaller, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
-
No products found
because this supplier's products are not listed.
Michael A. Augello, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or activating MACC constructs with 100uL of 1:1 Matrigel (Corning; cat. 354234) and cells (resuspended in 1x PBS ...
-
No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... then 1 µM all-trans retinoic acid (RA, STEMCELL Technologies, 72262) and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2 ...
-
No products found
because this supplier's products are not listed.
Danni Fan, Jing Yang, Nora Cronin, David Barford,
bioRxiv - Biochemistry 2022
Quote:
... GST-tagged GTPases were purified on high affinity GST resin (GenScript). The GST-tags were removed by PreScission protease and separated from the GTPase by a further gel filtration column ...
-
No products found
because this supplier's products are not listed.
Hasan Alhaddad, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Protein extracts were immunoblotted using antibodies against SERPINA3 (1:1000, Genetex), phospho-ERK (Thr202/Tyr204 ...
-
No products found
because this supplier's products are not listed.
K. Linda, et al.,
bioRxiv - Neuroscience 2024
Quote:
... primary antibody staining (ALDH1L1, Abcam #ab871170 1:250; S100B, Synaptic Systems #287006 1:250; GFP, Abcam #ab13970 1:1000; Stem121, Takara #Y40410 1:500) was performed in staining buffer (2% normal horse serum ...
-
No products found
because this supplier's products are not listed.
Muhammed Jamsheer K, et al.,
bioRxiv - Plant Biology 2020
Quote:
... HSP90-2 antibody (Heat shock protein 90-2 antibody; dilution: 1:5000; catalog no.: AS11 1629, Agrisera) was used as the loading control ...
-
No products found
because this supplier's products are not listed.
Denisa Bojkova, et al.,
bioRxiv - Microbiology 2022
Quote:
... Protein bands were visualised using IRDye-labeled secondary antibodies at dilution 1:40000 (LI-COR Biotechnology ...
-
No products found
because this supplier's products are not listed.
Parisa Kakanj, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Biotinylated proteins were visualized with α-Streptavidin-HRP antibody (Jackson ImmunoResearch, 1:2000. #016-030-084). For mass spectrometry ...
-
No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 0.5 mM RA and 1 mM 8-bromo-cAMP (Peprotech, 2354843) were added ...
-
No products found
because this supplier's products are not listed.
Danielle M. Spice, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 14 and 17 after RA treatment using QIAshredder (Qiagen, 79654) and RNeasy (Qiagen ...
-
No products found
because this supplier's products are not listed.
Bridget M Curran, et al.,
bioRxiv - Neuroscience 2024
Quote:
... rabbit anti Red Fluorescent Protein (RFP) polyclonal antibodies (Rockland Antibodies, 200-101-379, 1:1000). For NF staining ...
-
No products found
because this supplier's products are not listed.
Yabing Nan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Antibodies against the following proteins were used for western blotting: SAS10 (1:5000, BETHYL Laboratories ...
-
No products found
because this supplier's products are not listed.
Antoine Rebendenne, et al.,
bioRxiv - Microbiology 2021
Quote:
... Infected cells were labelled with a mixture of the mouse monoclonal antibody J2 against dsRNA (Scicons, diluted 1:400) and a rabbit polyclonal antibody directed against the spike protein (Sino Biological Inc, diluted 1:500) in PBS supplemented with 5% GS for 30 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Hongyi Wu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
-
No products found
because this supplier's products are not listed.
Bart C.J. Dirven, et al.,
bioRxiv - Neuroscience 2024
Quote:
... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... with a digital microscope camera (RA-MC120HD, Leica). The medium of the brown adipocytes was replaced with basal culture medium (high-glucose DMEM containing 20% FBS ...
-
No products found
because this supplier's products are not listed.
Hanjie Yu, et al.,
bioRxiv - Microbiology 2023
Quote:
... a rabbit polyclonal antibody to SARS-CoV-2 Spike protein (1:2000 dilution, Abclonal, China), a mouse monoclonal antibody to GAPDH (1:3000 dilution ...
-
No products found
because this supplier's products are not listed.
Rebecca L. Schmitz, et al.,
bioRxiv - Bioengineering 2023
Quote:
... NK cell activating medium was supplemented with 10 ng/mL IL-12 (Invivogen), 50 ng/mL IL-15 ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
... mice were anesthetized with 2.5% Isoflurane (RAS-4 Rodent Anesthesia system, Perkin Elmer) and 150 mg/kg body weight D-Luciferin-K-Salt (PJK GmbH ...
-
No products found
because this supplier's products are not listed.
Jesus Silvas, et al.,
bioRxiv - Microbiology 2021
Quote:
... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
-
No products found
because this supplier's products are not listed.
Meiling Zhao, Sung Eun Kwon,
bioRxiv - Neuroscience 2022
Quote:
... We report data from simultaneous calcium imaging and behavior from 5 SYNGAP1 WT mice (VgatCre/+; SynGAP+/+) and 5 Vgat-Het mice (VgatCre/+; SynGAPfl/+) (Jackson labs) with a C57BL/J6 background ...
-
No products found
because this supplier's products are not listed.
Marissa Lindman, et al.,
bioRxiv - Immunology 2024
Quote:
... Pan-flavivirus E protein antibody (1:100, VWR Enzo, 76285-044), GFAP (1:250 ...
-
No products found
because this supplier's products are not listed.
Jashodeep Datta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... αPD-1 antibody (BioXCell, Clone #BE0273 ...
-
No products found
because this supplier's products are not listed.
Coraline Chéneau, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... antibodies in Protein LoBind tubes (Eppendorf). Thereafter ...
-
No products found
because this supplier's products are not listed.
Changnan Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Forward genetic screens for fat-7p::GFP activating or suppressing mutants after ethyl methanesulfonate (EMS)-induced random mutagenesis were performed as described previously3,58 ...
-
No products found
because this supplier's products are not listed.
Kathryn H. Gunn, Saskia B. Neher,
bioRxiv - Biophysics 2023
Quote:
... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
-
No products found
because this supplier's products are not listed.
Lucas Bayonés, et al.,
bioRxiv - Physiology 2022
Quote:
... Transient transfections with the plasmid dynamin I K44A-EGFP (a dynamin-I GTPase-defective dominant-negative mutant) were performed using a Nucleofector 4D device (Lonza, Switzerland), according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Suchira Gallage, et al.,
bioRxiv - Physiology 2023
Quote:
... pLNC-ER: RAS vector and packaging vectors using 1mg/mL of linear 25 kDa polyethylenimine (PEI, Polysciences). 24 hours following transfection ...