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Citations for Electron Microscopy Sciences :
1 - 50 of 856 citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Forward genetic screens for fat-7p::GFP activating or suppressing mutants after ethyl methanesulfonate (EMS)-induced random mutagenesis were performed as described previously3,58 ...
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bioRxiv - Systems Biology 2022Quote: ... We then bound the chips to a 24×50×1.5mm glass slide (Fisherbrand 12-544-EP) after activating both surfaces for 10s at 100W in a plasma oven (EMS Quorum 1050X). We found that carefully optimizing plasma parameters and controlling humidity were critical for good binding ...
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bioRxiv - Cell Biology 2024Quote: ... Protein A conjugated with colloidal gold (1/30, Electron Microscopy Sciences) was added for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... prior to incubation with Gold-conjugated secondary antibodies (Transmission Electron Microscopy Sciences, 25100, 1:50) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... the grids were incubated with secondary antibodies conjugated to 10 nm or 6 nm gold particles (Cytodiagnostics, 1:20, and Electron Microscopy Sciences, 1:40) in PBS containing 0.1% gelatin at 21 °C for 1 h ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µL of protein was applied to Quantifoil grids (EMS) that were glow-discharged for 30s at 15 mA ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaned by standard acetone precipitation (EM grade acetone; EMS). The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 15nm or 18nm gold secondary antibodies (EMS). Grids were washed ...
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bioRxiv - Microbiology 2020Quote: ... Each protein was deposited on a CF-400-CU grid (Electron Microscopy Sciences) that had been plasma cleaned for 30 seconds in a Solarus 950 plasma cleaner (Gatan ...
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bioRxiv - Synthetic Biology 2020Quote: Purified protein samples applied to CF300-Cu carbon coated grids (Electron Microscopy Sciences) were prepared for negative stain transmission electron microscopy ...
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bioRxiv - Microbiology 2021Quote: ... After that, Samples were infiltrated in graded mixtures (1:3, 1:1, 3:1) of resin (EMS, Resin Mixture ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Biophysics 2024Quote: ... a 20-μL drop of 1% phosphotungstic acid (1%; Electron Microscopy Sciences SKU 19502-1), and three 20-μL drops of deionized water were sequentially placed on a piece of parafilm ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Neuroscience 2021Quote: ... the beads with enriched proteins were washed three times with 80% ethanol (Electron Microscopy Sciences Cat. #15055) followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were deposited on formvar/carbon-coated 200-mesh copper grids (FCF200-Cu, Electron Microscopy Sciences) for 1 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2.2µL drop of protein was deposited on a Quantifoil R1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Biochemistry 2022Quote: ... A 3.5 μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences) that had been glow discharged for 10 seconds using an easiGlow Glow Discharge Cleaning System (PELCO) ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2024Quote: ... A 3.5-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Cleaning System (Ted Pella) ...
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bioRxiv - Plant Biology 2023Quote: ... nrpe1-1 (EMS), and drm1-2 (SALK_031705 ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.1% tanic acid (EMS),1% glutaraldehyde (EMS) in anhydrous acetone (EMS ...
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bioRxiv - Neuroscience 2020Quote: ... Girds were immersed in 15µl beads of secondary antibody (Electron microscopy sciences, goat-anti-rabbit 15 nm ...
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bioRxiv - Cell Biology 2020Quote: ... atgl-1(xd310) and atgl-1(xd314) were generated by EMS. xdIs182 (Prab-3::Cre/Podr-1::RFP ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then embedded in 1:1 Embed-812 (EMS 14120):acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Immunology 2024Quote: ... A 3.5-μL drop of protein was deposited on a Quantifoil Au-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 40 seconds using a PELCO easiGlow Cleaning System (Ted Pella) ...