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Citations for Addgene :
1 - 50 of 1964 citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Ras GTPase-activating protein-binding protein 1 (G3BP1) phage UbiC G3BP1-GFP-GFP was a gift from Jeffrey Chao (Addgene plasmid # 119950 ...
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bioRxiv - Microbiology 2024Quote: ... and Arf GTPases (Addgene #49578, #39556) using Lipofectamine LTX ...
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bioRxiv - Cell Biology 2022Quote: ... RA was PCR amplified from RA-NES (gift from R. Campbell, Addgene plasmid #61019) (Ding et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was replaced with H-Ras V12 cDNA from pBABE-puro H-Ras V12 (Addgene #9051) to generate pWZL H-Ras V12 blast.
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bioRxiv - Cancer Biology 2020Quote: ... H-Ras V12 (Addgene plasmid # 18749), and ST (Addgene plasmid # 8583 ...
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bioRxiv - Cancer Biology 2022Quote: ... and K-RAS(V12) (Addgene 9052) viruses as previously described36,37 ...
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bioRxiv - Bioengineering 2024Quote: ... and FRB-CFP-Ras (Addgene ID: 87451) are available on Addgene ...
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bioRxiv - Biochemistry 2020Quote: ... Retroviral vectors pBabe-puro Ras V12 (Addgene #1768) and pWZL-Blast-myc (Addgene #10674 ...
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bioRxiv - Neuroscience 2023Quote: pCS2(xtFapb7):mTagBFP2-Ras: BFP from pBAD-mTagBFP2 (Addgene plasmid # 34632 ...
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bioRxiv - Developmental Biology 2023Quote: ... A sgRNA targeting the SYNGAP1 c.1507C site was cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988). The sgRNA together with the HDR template to introduce the 1507C>T mutation ...
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bioRxiv - Developmental Biology 2023Quote: A sgRNA targeting the arginine finger region of SYNGAP1 was cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988). This ...
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bioRxiv - Developmental Biology 2023Quote: A sgRNA targeting the patient-specific mutation in SYNGAP1 was cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988). This ...
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A quantitative characterization of early neuron generation in the developing zebrafish telencephalonbioRxiv - Developmental Biology 2023Quote: pCS2-mKate2-ras was a gift from Caren Norden (Addgene #105938) (Weber et al. ...
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bioRxiv - Microbiology 2021Quote: ... Rab GTPase dominant negative constructs [mCherry-Rab5DN(S34N) and dsRed-Rab7DN] were purchased from Addgene (76, 77). For consistency ...
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bioRxiv - Neuroscience 2024Quote: ... a virus coding for membrane bound GFP and mCherry conjugated to the synaptic protein Synaptophysin was used (AAV5-hsyn-mGFP-2A-synaptophysin-mRuby, Addgene). To visualize VTA/SNc neurons receiving direct SC input ...
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bioRxiv - Biochemistry 2021Quote: ... The RAS clone collection was a gift from Dominic Esposito (Addgene kit 1000000070). GST tagged RAF1-RBD (GST-RAF-RBD ...
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bioRxiv - Cell Biology 2023Quote: ... RA-Sec61β and GB-Dcp1 were gifts from Gia Voeltz (Addgene plasmid #153978, #153979). Halo-OSBP was created by amplifying OSBP from pLJM1-FLAG-GFP-OSBP (Addgene #134659 ...
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A quantitative characterization of early neuron generation in the developing zebrafish telencephalonbioRxiv - Developmental Biology 2023Quote: ... pCS2-LSSmOrange-ras was generated by replacing mKate2 with LSSmOrange coding sequence (Addgene #37131). pCS2-Cre-ERT2 was generated by inserting PCR-generated ERT2 sequence (with pBigTB-CreERT2 (Addgene #149434 ...
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bioRxiv - Neuroscience 2022Quote: ... was injected unilaterally in the A13 while for DREADD experiments viruses for reversibly activating (AVV8-Syn-hM3D-Gq-mCherry; Addgene # 44361), inhibiting (AVV8-hSyn-hM4D-Gi-mCherry ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of plasmid (pBABE-puro, Addgene #1764 or pBabe-puro Ras V12, Addgene #1768) was added to the FuGENE + OPTI-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... RAS variants were obtained from the Target Accelerator Pan-Cancer Mutant Collection (Addgene, cat. 1000000103). Individual liquid cultures of bacteria expressing pDONR plasmids encoding each of the 26 RAS variants included in our assay were pooled according to their OD600 to achieve a balanced representation ...
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bioRxiv - Neuroscience 2023Quote: pCS2(xtFapb7):mTagBFP2-Ras: BFP from pBAD-mTagBFP2 (Addgene plasmid # 34632 ; http://n2t.net/addgene:34632 ; RRID:Addgene_34632) (Subach et al. ...
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bioRxiv - Developmental Biology 2023Quote: zld, grh, or twi cDNA (isoforms RB, RH, and RA, respectively) were cloned into pMT-puro plasmid (Addgene) via Gibson cloning (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... SpCas9 and trans-activating CRISPR RNA (tracrRNA) were expressed using the pX459 plasmid (a gift from Dr. Feng Zhang, Addgene plasmid #62988,(Ran et al. 2013)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled clones were expanded before being transfected with Not1-linearized pWZL-hygro H-Ras V12 plasmid (Addgene plasmid # 18749) and selected with hygromycin for 2-4 weeks to generate stable clone ...
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bioRxiv - Developmental Biology 2023Quote: We cloned a 691 bp fragment from the kni regulatory region (kni- RA) in pHP-Dest-eGFP (Plásmido #24566 Addgene) (Boy et al. ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Biophysics 2022Quote: ... lentivirus was generated using pcDNA/TO/GFP H-Ras V12 (gift of Yasuyuki Fujita)[43] and pLenti CMV TetR Blast (Addgene plasmid 17492), and cells were selected based on fluorescent expression.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... NotI/XbaI PCR fragment of coding sequences of csw-RA was inserted into the NotI/XbaI sites of pJFRC28-10XUAS-IVS-GFP-p10 vector (Addgene Plasmid #36431), and then the construct was inserted into attp2 site.
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bioRxiv - Neuroscience 2020Quote: ... we injected ∼0.5 μl of anterograde trans-synaptic virus (AAV1-hsyn-Cre, Addgene Cat# 105553-AAV1) into the right DMS (bregma 0 mm ...
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bioRxiv - Biochemistry 2021Quote: The RAS binding domain of C-RAF kinase (RAF-RBD) (Brtva et al., 1995) and full-length human RASSF5 from the RAS clone collection were obtained from Addgene (Raf1-RBD: #13338, RASSF5: #70545). The RAS clone collection was a gift from Dominic Esposito (Addgene kit 1000000070) ...
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bioRxiv - Immunology 2023Quote: Pseudovirus expressing Wuhan-Hu-1 SARS-CoV-2 S protein were produced by co-transfection of plasmids encoding a GFP protein (Addgene, 11619), a lentivirus backbone (VRC5602 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pEGFP-LC3 (EGFP-LC3: microtubule associated protein 1 light chain 3 fused with enhanced green fluorescent protein) (Addgene, 24920) at 0.1 µg of the plasmid (in 50 µl Opti-MEM® Reduced Serum Media ...
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bioRxiv - Neuroscience 2023Quote: For Synaptic plasticity experiments C57Bl/6-Jax mice were injected with AAV-Chronos (pAAV-Syn-Chronos-GFP, Addgene 59170-AAV5) virus in area S2 at locations and concentrations given in Table M2.
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bioRxiv - Neuroscience 2020Quote: ... was mediated by a co-administration of a trans-synaptic CAV2-Cre virus (Plateforme de Vectorologie de Montpellier (PVM)) and a pAAV8-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene; plasmid #44362) while that of the mChrerry reporter alone was mediated by co-administration of the CAV2-Cre virus and a pAAV8-hSyn-DIO-mCherry virus (Addgene ...
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bioRxiv - Immunology 2020Quote: ... and the full-length protein (IFT20, aa 1-132) in-frame with the tags into the pEGFP-N1 (#6085-1 Addgene) and pGEX-6P-2 vectors (#27-4598-01Addgene) ...
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bioRxiv - Biochemistry 2024Quote: ... The tag-free wildtype (Wuhan-hu-1) nucleocapsid protein plasmid was purchased from Addgene (#177937), and the plasmid encoding the Strep-tagged nucleocapsid protein was a kind gift from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Vimentin was first amplified from from human cDNA encoding isoform 1 (VIM-201, protein 466aa) and cloned in to the KIF1A(1-365)-GFP-SspB plasmid (Addgene #174629) (Nijenhuis et al. ...
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bioRxiv - Biochemistry 2024Quote: Human interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) gene (Gene ID: 3434) was obtained from Addgene in the plasmid vector pET28a_IFIT1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with Protein A/G-MNase fusion protein (Addgene, Cat #123461) at 700 ng/mL for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...
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bioRxiv - Neuroscience 2024Quote: ... a 448 bp human synapsin-1 promoter element driving the neuronal specific expression of 4) the fluorescent protein mTagBFP2 (Addgene plasmid #191566 pLKO.1-TRC mTagBFP2) fused to the plasma membrane targeting sequence CAAX2 (Addgene plasmid #162247 ...
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bioRxiv - Cell Biology 2020Quote: ... and WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) cDNA was a gift from Noboru Mizushima (Addgene plasmid # 38272) (Itakura & Mizushima ...
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bioRxiv - Cancer Biology 2024Quote: ... Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01, Addgene). Mutants of His- ...
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bioRxiv - Systems Biology 2022Quote: ... or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene) to account for differences in infection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... to label hyaluronic acid-based ECM we designed AAV expression vector carrying link protein hyaluronan and proteoglycan link protein 1 (HAPLN1, Gene ID: 12950) fused with mScarlet subcloned from the plasmid pCytERM_mScarlet_N1 (Addgene plasmid # 85066). Clones were verified by sequencing analysis and used for the production of adeno-associated particles as described previously (Mitlöhner et al ...
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bioRxiv - Biochemistry 2022Quote: ... full-length FUS with an N-terminal histidine tag and maltose-binding protein fusion (pTHMT FUS 1-526, AddGene #98651), and FUS RGG3 with N-terminal histidine tag and MBP fusion (pTHMT FUS RGG3 (453-507) ...
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bioRxiv - Cell Biology 2024Quote: ... Short hairpin RNAs (shRNAs) targeting WSB2 or BCL-2 family proteins were subcloned into the pLKO.1 puro vector (Addgene) for gene KD ...
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bioRxiv - Plant Biology 2024Quote: ... The CDS of the prey protein (CLPS1) was cloned and inserted into the pET His6 MBP TEV LIC cloning vector (1 M) (#29656, Addgene) cloning vector to generate fusion constructs with an MBP tag ...
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Hippocampal efferents to retrosplenial cortex and lateral septum are required for memory acquisitionbioRxiv - Neuroscience 2020Quote: ... enhanced yellow fluorescent protein (eYFP) or enhanced green fluorescent protein (eGFP): AAV1.CaMKIIa.eNpHR3.0.eYFP.WPRE.hGH (Addgene, #26971), AAV1.CaMKII.eYFP ...