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No products found
because this supplier's products are not listed.
Li Chang, et al.,
bioRxiv - Plant Biology 2019
Quote:
... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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No products found
because this supplier's products are not listed.
Efi Makdasi, et al.,
bioRxiv - Immunology 2021
Quote:
The following His-tagged recombinant proteins were purchased from Sino Biologicals: B.1.1.7 rS1-SARS-CoV-2 spike S1 [Δ 69-70 ...
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No products found
because this supplier's products are not listed.
Chen Liu, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Konstantinos Nakos, et al.,
bioRxiv - Cell Biology 2019
Quote:
Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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No products found
because this supplier's products are not listed.
Li Chang, et al.,
bioRxiv - Plant Biology 2019
Quote:
... His-tagged recombinant protein was purified by TALON Superflow (GE Healthcare Life Sciences, Pittsburgh, PA, USA) according to the manufacturer’s description ...
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No products found
because this supplier's products are not listed.
Mingu Kang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
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No products found
because this supplier's products are not listed.
Joy S. Xiang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
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No products found
because this supplier's products are not listed.
Tatiana P. Soares da Costa, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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No products found
because this supplier's products are not listed.
Sieglinde De Cae, et al.,
bioRxiv - Microbiology 2023
Quote:
... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
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No products found
because this supplier's products are not listed.
Anthony J. Rabe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The hexahistidine tag and Sso7dmut were cleaved from HIV-1 IN by addition of his-tagged sortase and GGGC peptide (GenScript). The reaction was exposed to nickel resin to remove sortase and any residual uncleaved IN ...
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No products found
because this supplier's products are not listed.
Daniele Tedesco, et al.,
bioRxiv - Biophysics 2021
Quote:
... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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No products found
because this supplier's products are not listed.
Sarah Wazir, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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No products found
because this supplier's products are not listed.
Zheqiong Yang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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No products found
because this supplier's products are not listed.
Tianhu Sun, et al.,
bioRxiv - Plant Biology 2022
Quote:
... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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No products found
because this supplier's products are not listed.
Mitro Miihkinen, et al.,
bioRxiv - Cell Biology 2021
Quote:
Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
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No products found
because this supplier's products are not listed.
David G. Saliba, et al.,
bioRxiv - Immunology 2019
Quote:
His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ...
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No products found
because this supplier's products are not listed.
Jorge El-Azaz, et al.,
bioRxiv - Plant Biology 2019
Quote:
... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
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No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
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No products found
because this supplier's products are not listed.
Jessica N. Spradlin, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Recombinant Flag-tagged RNF114 was used as bait to precipitate pure recombinant p21 (Origene Technologies Inc. ...
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No products found
because this supplier's products are not listed.
Wei Kong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ...
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No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Kathleen M. Mulvaney, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the complex was biotin-labeled via incubation with recombinant sortase A5 enzyme (Active Motif) and a custom tri-glycine biotin peptide (Thermo Fisher) ...
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No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
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No products found
because this supplier's products are not listed.
M. T. Gyparaki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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No products found
because this supplier's products are not listed.
Jianan Zhang, et al.,
bioRxiv - Biophysics 2024
Quote:
... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Farah Haque, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
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No products found
because this supplier's products are not listed.
Tania Christova, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant GST-tagged LTK (SignalChem #L11-11G) was incubated in the absence or in the presence of an equal amount of recombinant His-tagged IGF-1R (SignalChem #I02-11H ...
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No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... or DIV12 neurons in the presence of recombinant protein (see recombinant protein below) or C1q-blocking antibody (Recombinant Anti-C1q antibody (ab182451)) / IgG (Normal Rabbit IgG,Cell Signaling Technology). For LPS induced activation of microglia ...
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No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
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No products found
because this supplier's products are not listed.
Zeynep Tarcan, et al.,
bioRxiv - Biochemistry 2021
Quote:
Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
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No products found
because this supplier's products are not listed.
Nurit P. Azouz, et al.,
bioRxiv - Immunology 2023
Quote:
... glycerol) with 250 ng total protein from cell extracts or 120 ng recombinant GST-tagged OVOL1 protein (Abnova #H00005017-P01) and non-fluorescent competitor containing the wild-type or mutant binding site for 30 minutes at room temperature and cross-linked at 120 mJ/cm2 ...
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No products found
because this supplier's products are not listed.
Jason S Presnell, William E Browne,
bioRxiv - Developmental Biology 2021
Quote:
... Recombinant Cas9 protein (PNA Bio) and sgRNAs were injected at concentrations of 400 ng/µl of Cas9 protein and 100 ng/µl for each sgRNA ...
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No products found
because this supplier's products are not listed.
Meisam Nosrati, et al.,
bioRxiv - Biochemistry 2019
Quote:
... His-tagged RmtC proteins were detected by immunoblotting with a rabbit anti-6×His antibody (α6×His; Proteintech; 10001-0-AP) overnight at 4 °C ...
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Cat# Sortase-162,
200µg : USD $1873
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
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No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Christin Naumann, et al.,
bioRxiv - Plant Biology 2021
Quote:
... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2020
Quote:
... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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No products found
because this supplier's products are not listed.
Roshina Thapa, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
The bacterial expression vectors pReceiver-B11 for His-NSMCE2 and His-PFDN2 recombinant protein expression were purchased from Genecopoeia, Inc (Rockville ...
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No products found
because this supplier's products are not listed.
Yoshiaki Kamiyama, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL, NY). In addition ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...