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No products found
because this supplier's products are not listed.
Brian G. Peterson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... using the sortase A (P94R/D160N/D165A/K190E/K196T) pentamutant56 and Sulfo-Cy5-Maleimide (Lumiprobe) coupled to a Gly-Gly-Gly-Cys peptide (Genscript) ...
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No products found
because this supplier's products are not listed.
Johan M.S. van der Schoot, et al.,
bioRxiv - Bioengineering 2019
Quote:
... containing 1 (Fab’) or 2 (mAb) molar equivalents of tri-mutant sortase A Δ59 (3M srt) (23) ...
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No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
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No products found
because this supplier's products are not listed.
Xiaoling Qiang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant proteins were tested for LPS content by the chromogenic Limulus amebocyte lysate assay (Endochrome; Charles River), and the endotoxin content was less than 0.01 U per microgram of recombinant proteins.
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Menglin Shi, Lei Zhao, Yong Wang,
bioRxiv - Plant Biology 2021
Quote:
The recombinant HPRs was purified with HIS-Select nickel affinity gel filler (CoWin Biosciences, Beijing, China). Briefly ...
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No products found
because this supplier's products are not listed.
Bhaskar Saha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... All GST-tagged proteins were expressed in Escherichia coli SoluBL21 (Genlantis). GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01) ...
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No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
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No products found
because this supplier's products are not listed.
Xiaofei Yang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The block was aspirated and anti-DNA/RNA G-quadruplex Mouse Fab fragment antibody (BG4 antibody, His-Tagged, Lambda antibody AB00174-1.6, Absolute Antibody) with a dilution of 1:300 in 1× PBS with 2% BSA was added to each slide and incubated in a dark (5) ...
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No products found
because this supplier's products are not listed.
Nils Stührwohldt, et al.,
bioRxiv - Plant Biology 2020
Quote:
... His6-tagged proteins by a monoclonal mouse α–His6 antibody from Dianova (Hamburg, Germany), and Flag-tagged proteins by α–Flag antibody directly coupled to horseradish peroxidase (Taufkirchen ...
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No products found
because this supplier's products are not listed.
Francesca Curreli, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Various concentrations of biotinylated recombinant SARS-CoV-2 Spike RBD protein (Bioss Antibodies, Woburn, MA) dissolved in water were manually printed onto the Chip with Biodo bioprinting at 40% humidity via biotin-avidin conjugation ...
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No products found
because this supplier's products are not listed.
Thi K. Tran-Nguyen,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant Human GRP78 protein (Catalog No. SPR-119) were purchased from StressMarq Biosciences (Victoria, BC, Canada). Four different lots (lot#1101 ...
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Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and...
Cat# LS006361,
10 ku, $200.00
Ask
Meiling Zhang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
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No products found
because this supplier's products are not listed.
Reuben A. Saunders, et al.,
bioRxiv - Biophysics 2020
Quote:
... our ssrA-tagged titin protein was reacted at room temperature with three equivalents of ATTO 575Q maleimide (ATTO-TEC; GMBH) for 30 min in buffer A supplemented with 5 mM Tris (pH 8.0) ...
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No products found
because this supplier's products are not listed.
Yamini Ravichandran, et al.,
bioRxiv - Cell Biology 2023
Quote:
... plasmids encoding the GFP- or mCherry tagged protein were microinjected into wound border astrocytes plated on glass bottom dishes (MatTek) and scratched 1h before microinjection ...
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No products found
because this supplier's products are not listed.
Clàudia Salat-Canela, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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No products found
because this supplier's products are not listed.
Sophie J. Hesketh, et al.,
bioRxiv - Cell Biology 2022
Quote:
For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
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No products found
because this supplier's products are not listed.
Siran Zhu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 150 pmol of 6×His-streptavidin (ProteoGenix) was mixed with 5 μl of sieved beads (10% ...
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No products found
because this supplier's products are not listed.
Ahmed Seddek, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Purified recombinant human TOP1 (TopoGEN) was used as positive control.
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No products found
because this supplier's products are not listed.
Dorothea Höpfner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... samples containing 100 ng recombinant protein were run over a 5 μm Jupiter C4 300Å LC column (Phenomenex, Torrance, California) using the 1260 Infinity LC system (Agilent Technologies ...
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No products found
because this supplier's products are not listed.
Selena Alonso, et al.,
bioRxiv - Immunology 2020
Quote:
... A total of 16 recombinant proteins were selected for the analysis: diphtheria toxoid (Corynebacterium diphtheriae, Alpha Diagnostic DTOX15-N-500), tetanus toxin (Clostridium tetani ...
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No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
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No products found
because this supplier's products are not listed.
Junjie Xu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The probe was tagged to opal 570 dye from Akoya Biosciences (Marlborough ...
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No products found
because this supplier's products are not listed.
Anil H. Kadam, et al.,
bioRxiv - Bioengineering 2022
Quote:
... recombinant hu TGF-β1(ProSci, Poway, CA), anti-TGF-β1 IgY-Biotin (R&D Systems ...
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No products found
because this supplier's products are not listed.
Yukiko Yamaguchi, et al.,
bioRxiv - Immunology 2022
Quote:
... containing 100 U/mL recombinant human IL-2 (rhIL-2, Novartis Oncology) and 0.5 ng/mL recombinant human IL-15 (rhIL-15, CellGenix). For CAR lentiviral transduction ...
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No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
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No products found
because this supplier's products are not listed.
Anne-Marinette Cao, et al.,
bioRxiv - Biophysics 2021
Quote:
The pcDNA plasmid encoding SNAP-tagged human mGluR2 was a gift from Cisbio Bioassays (Codolet ...
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No products found
because this supplier's products are not listed.
Álvaro Inglés-Prieto, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were treated with recombinant human GDNF (Shenandoah Biotechnology) and human GFRα1 (R&D Systems ...
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No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
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No products found
because this supplier's products are not listed.
Nawar Naseer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days to promote differentiation into hMDMs ...
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No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron. In vitro transcribed single guide RNAs (sgRNAs ...
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No products found
because this supplier's products are not listed.
Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
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No products found
because this supplier's products are not listed.
Jonathan M Downie, et al.,
bioRxiv - Genetics 2019
Quote:
... HEK293T cells were incubated in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HI FBS, Genesee Scientific), and 1% pen/strep (P/S ...
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No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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No products found
because this supplier's products are not listed.
Jamin Jung, et al.,
bioRxiv - Cell Biology 2021
Quote:
... a spinning disk confocal microscope (UltraVIEW VOX, Perkin-Elmer) equipped with an oil immersion objective Plan-Apochromat 100x/1.57 Oil-HI DIC (Carl Zeiss) was used ...
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No products found
because this supplier's products are not listed.
April H. Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
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No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
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No products found
because this supplier's products are not listed.
Lindsay G. Serene, et al.,
bioRxiv - Microbiology 2023
Quote:
L929 fibroblasts were rapidly thawed in a 37°C water bath before being washed once in DMEM and 10% HI-FBS and transferred to a T-25 tissue culture treated flask (Sarstedt, Nümbrecht, Germany) in 10 mL DMEM and 10% HI-FBS with 100 U/mL penicillin/streptomycin ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
C López-Haber, et al.,
bioRxiv - Cell Biology 2020
Quote:
Recombinant lentiviruses encoding shRNAs were produced by co-transfection of 293T cells (obtained from American Type Culture Collection, Mannassas, VA) with packaging vectors pDM2.G and pSPAx2 using calcium phosphate precipitation (Marks et al ...
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No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
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No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
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No products found
because this supplier's products are not listed.
Yeongjoon Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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No products found
because this supplier's products are not listed.
Lien D. Nguyen, et al.,
bioRxiv - Neuroscience 2022
Quote:
Total protein was extracted using RIPA buffer (Boston Bioproducts) supplemented with Complete ...
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No products found
because this supplier's products are not listed.
Joël S. Bloch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Protein Complex Suite (NeXtal) and PEG/Ion Suite (Hampton Research). Precipitant solution from crystallization screen was dispensed at 1:1 macromolecular complex/precipitant solution ratio in a total drop volume of 400 nL at room temperature ...
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No products found
because this supplier's products are not listed.
Claire S. Chung, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... protein samples were spotted on carbon-coated Formvar grid (Ted Pella). The samples were stained with nanoW/uranyl acetate before air drying ...