Labshake search
Citations for Becton, Dickinson and Company :
1 - 50 of 364 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
-
bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells containing Atto488-tagged protein (530 nm) were detected by flow cytometry on a FACSCalibur (BD Biosciences ...
-
bioRxiv - Cancer Biology 2020Quote: FITC-tagged AnnexinV (BD-Biosciences), PI(Sigma ...
-
bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
-
C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
-
bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
-
bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
-
bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
-
bioRxiv - Immunology 2023Quote: ... and recombinant IFNγ (BD Pharmingen) as a standard ...
-
bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
-
bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
-
bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
-
bioRxiv - Genetics 2020Quote: ... and the amounts of recombinant ACE2-GFP and dACE2-GFP proteins in lysates were quantified by densitometry analysis (Imagelab, BD Biosciences) of Western blots ...
-
bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
-
bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Systems Biology 2022Quote: Recombinant human EGF was purchased from BD Biosciences (San Jose ...
-
bioRxiv - Cancer Biology 2022Quote: ... Recombinant human EGF was purchased from BD Biosciences ...
-
bioRxiv - Systems Biology 2021Quote: ... and anti-active Caspase3- V450 tagged antibody)) were procured from BD Biosciences.
-
bioRxiv - Neuroscience 2023Quote: ... Alexa-488 tagged cytochrome c antibody (BD Biosciences, Franklin Lakes, USA), as well as the secondary antibody goat-anti-rabbit Alexa 568 (1:1000 ...
-
bioRxiv - Immunology 2024Quote: ... the cells were tagged using a Sample Multiplexing Kit from BD. Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen, 556104). We generated truncation plasmids based on full-length proteins ...
-
bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
-
bioRxiv - Immunology 2022Quote: ... Cells from each subjzect were sample tagged using Sample Multiplexing Kit (BD Biosciences) which contains oligonucleotide cell labeling ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then stained for fluorophore-tagged antibodies against Ly6G (BD Bioscience # 5605991), phospho c-Jun (Ser73 ...
-
bioRxiv - Immunology 2021Quote: ... recombinant human IL-12 (10 ng/ml, BD Biosciences) was added on days 5 ...
-
bioRxiv - Physiology 2021Quote: ... Human recombinant TNFα (10-100 ng/ml, BD Biosciences), BAPTA-AM (100 μM ...
-
bioRxiv - Cell Biology 2021Quote: ... Other reagents include: recombinant basement membrane (rBM; BD Biosciences), Cultrex 3D Culture Matrix Laminin I (R&D systems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... CAR-T cells were stained with fluorescent tagged anti-CD3 (BD Biosciences, Cat#557851), anti-NGFR (Miltenyi Biotech ...
-
bioRxiv - Systems Biology 2019Quote: ... Soluble mouse recombinant IL10 and IFNγ were procured from BD Biosciences (San Diego ...
-
bioRxiv - Microbiology 2021Quote: Recombinant human TNF-α (Cat. # 554618) was purchased from BD. Biosciences ...
-
bioRxiv - Bioengineering 2022Quote: ... Recombinant baculovirus was generated using the Baculogold system (BD Biosciences) and Trichoplusia ni cells were infected for protein production ...
-
bioRxiv - Physiology 2023Quote: ... We purchased human recombinant TNFα (10 ng/ml) from BD Biosciences (San Jose ...
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor (EGF; BD Biosciences), human recombinant basic fibroblast growth factor (bFGF ...
-
bioRxiv - Molecular Biology 2023Quote: ... Recombinants were selected for on LB agar (1.0% bactotryptone (BD), 0.5% yeast extract (Alfa Aesar) ...
-
bioRxiv - Immunology 2020Quote: ... in the presence of recombinant IL2 and BD GolgiPlug (BD Biosciences) for 12 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... Human recombinant growth factors PDGF and EGF were purchased from BD Biosciences ...
-
bioRxiv - Microbiology 2020Quote: ... PAO1 recombinant strains were selected on Pseudomonas Isolation Agar (PIA, BD) containing 100 µg ml−1 gentamicin ...
-
bioRxiv - Bioengineering 2023Quote: ... Recombinant TNFα was purchased from R&D Systems (cat #: 210TA100/CF) and recombinant MCP-1 was purchased from BD Biosciences (cat # ...
-
bioRxiv - Cell Biology 2022Quote: ... and fluorescently tagged antibodies to label MuSCs: integrin-α7-PE (Ablab) and CD34-Alexa647 (BD Biosciences). The cell suspension was sorted by FACS to deplete non-muscle lineage cells and enrich MuSCs (Lin-/integrin-α7+/CD34+/ GFP-) ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclei were then tagged with propidium iodide and purified using flow-assisted cell sorting (FACS; BD FACS Aria II ...
-
bioRxiv - Microbiology 2020Quote: ... Tn5 insertion recombinants were selected on Mueller Hinton (MH) agar (BD Bacto) supplemented with 7 μg.mL-1 kanamycin ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were labeled with fluorescently tagged antibodies and then were analyzed using BD LSR II (BD Bioscience) and FlowJo Software (FlowJo ...
-
bioRxiv - Cancer Biology 2020Quote: Antibodies utilized were directed against TFRC (PE-tagged anti-CD71 clone M-A712, BD Biosciences, San Jose, CA) and Glycophorin A (PE-tagged-anti-CD235a clone REA175 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the pCRKL (Y207) specific Alexa Fluor® 647 tagged antibody (cat#560790, Becton, Dickinson and Company, NJ, US) was used as per the manufacturer’s protocol in a Beckman Coulter Gallios flow cytometer (Beckman Coulter Inc. ...
-
bioRxiv - Neuroscience 2021Quote: ... All expression constructs were tagged C-terminally with hexahistidine tags in the baculoviral transfer vector pAcGP67A (BD Biosciences). Proteins were purified using Ni-NTA agarose (Qiagen ...