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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
His-tagged SSRP1 was PCR amplified from SSRP1 pReceiver (GeneCopoeia) using primers encoding an N-terminal His tag and NdeI/NotI cut sites for cloning into pET22b ...
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No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... his-tagged extracellular domain of the growth hormone receptor (GHR, BioVision 7477-10) was immobilized on the Ni-NTA sensor tip by dipping the tips in a 2.5 μg/ml solution of GHR for 400 s ...
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No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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No products found
because this supplier's products are not listed.
Rui Fujii, et al.,
bioRxiv - Cell Biology 2024
Quote:
Sch91–183–6xHis was detected using mouse monoclonal anti-His antibody (AB10597733, cat. # D291–3; MBL International, Woburn, MA, USA). A C-terminal fragment of Sch9–5HA was detected using a mouse monoclonal anti-HA antibody (AB2565007 ...
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No products found
because this supplier's products are not listed.
Amit Rahi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HRP-tagged mouse/rabbit antibodies (Jackson laboratories) were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher).
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No products found
because this supplier's products are not listed.
Marianna Ioannou, et al.,
bioRxiv - Immunology 2021
Quote:
... 500nM and 1uM of human recombinant Histone 3 (Cayman chemical) and 1ng/ml ...
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No products found
because this supplier's products are not listed.
Young Rock Chung, et al.,
bioRxiv - Immunology 2023
Quote:
... Goat anti-mouse IgG antibody tagged with streptavidin-HRP (Southern Biotech, 7105-05) was diluted 1:400 in blocking buffer and incubated for 1 hr at room temperature ...
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No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
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No products found
because this supplier's products are not listed.
Alexandre B. Leitão, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Hourinaz Behesti, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 100 ng/ml recombinant human/mouse FGF8b (Tocris) between days 1-11 (range tested ...
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No products found
because this supplier's products are not listed.
Matthew D. Cain, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant mouse IFNα (PBL Assay Science, 12100-1) was administered intranasally ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Filippo Vascon, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
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No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
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No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... goat anti-mouse IgG h+l HRP-tagged antibody (Bethyl Laboratories) diluted 1:20000 ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Keval Bollavaram, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals). Membranes were imaged with a LI-COR Odyssey scanner.
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No products found
because this supplier's products are not listed.
Jason Wallach, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and a Venus-tagged N-terminal β-arrestin2 using 3:1 ratio of TransiT-2020 (Mirus). 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs) ...
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No products found
because this supplier's products are not listed.
Casimir Bamberger, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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No products found
because this supplier's products are not listed.
Ivan Martinez-Valbuena, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant α-synuclein (rPeptide) was thawed from −80 ◦C storage ...
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No products found
because this supplier's products are not listed.
Máté Fischer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
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No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
because this supplier's products are not listed.
Huilei Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... An adenovirus used to overexpress C-terminal His-tagged LOXL2 was purchased from Vector Biolabs. Vitamin E (α-Tocopherol ...
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No products found
because this supplier's products are not listed.
Priyadharshini Devarajan, et al.,
bioRxiv - Immunology 2022
Quote:
... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
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No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were pre-incubated with 3 nM of CypHer5E-tagged anti-VGAT antibodies (131 103CpH, Synaptic Systems, Germany) in the culture medium and incubated in the 5% CO2 incubator for 2 h at 37°C to specifically label inhibitory synaptic vesicles in presynaptic terminals ...
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No products found
because this supplier's products are not listed.
Belloy Marcy, et al.,
bioRxiv - Animal Behavior and Cognition 2024
Quote:
... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
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No products found
because this supplier's products are not listed.
Gangming Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The degron-tagged L1 larvae were plated on NGM plates with 500 μM indole-3-acetic acid (IAA; Alfa Aesar, 10171307). Worms were placed on IAA plates and collected at young adult stage for further analysis.
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Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and...
Cat# LS006361,
10 ku, $200.00
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Meiling Zhang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
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No products found
because this supplier's products are not listed.
Gongshi Bai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... FAM-tagged TelG probe (PNA Bio F1005) was diluted (1:100 ...
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No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
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No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...