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Citations for Polysciences :
1 - 50 of 172 citations for Recombinant Mouse Dickkopf Homolog 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with 8 μ of plasmid DNA (SF-tagged LRRK2 WT, or SF-tagged LRRK2 G2019S) per 10cm2 dish using polyethyleneimine (Polysciences) solution ...
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bioRxiv - Neuroscience 2021Quote: ... 2500 sterilized FITC-tagged microspheres (Polysciences Inc., PA, USA) suspended in a 100µl of PBS were slowly injected into the CCA using a 33G hypodermic needle (TSK Laboratory International ...
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bioRxiv - Immunology 2023Quote: ... or Myc-tagged constructs by using polyethylenimine (PEI, Polysciences, 23966). After 24 hr later ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with the Strep-tagged constructs using polyethylenimine (PEI, Polysciences), in a ratio of 3:1 for PEI:DNA ...
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bioRxiv - Genetics 2019Quote: ... then transfected with the corresponding SF-TAP-tagged DNA constructs using PEI reagent (Polysciences) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Bioengineering 2023Quote: ... separate samples of 10 kPa solution were made substituted with Nile blue-tagged acrylamide (Polysciences Inc. #25395-100) at 1:1000.
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bioRxiv - Biophysics 2021Quote: ... Cells in a single dish were transfected with 1.25 μg of c-term EGFP tagged hTMEM175 plasmid using 3.75 μg of PEI 25 k (Polysciences, Inc). Electrophysiological recordings were performed 48-72 hr after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: HEK293TR cells were transfected with pcDNA4/TO encoding SNAP-tagged β2AR and A2AR with PEI transfection reagent (Polysciences Europe GmbH) (PEI:DNA ratio of 3:1) ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T were seeded in 10-cm dishes then transfected with 10 ug of myc-tagged hnRNPA1 and hnRNP A1dm mutant using polyethyleneimine (PolySciences). Cells were treated with 100 uM 4-SU 16 hours prior to UV-crosslinking ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were co-transfected with both plasmids containing the Venus fragments-tagged bait and prey ORF (100 ng for each) which were pre-mixed and incubated with polyethylenimine (PEI) (Polysciences Inc.) and OptiMEM (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... by pre-mixing C-terminal AVI-tagged ZM197-ZM233V1V2 or C-terminal AVI-tagged CAP256-wk34c80-RnS-3mut-2G with a furin expression plasmid using Polyethylenimine (PEI) 40K (Polysciences, Inc.) or Turbo293 (Speed BioSystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then grown to optical density of 1.2×106 cells/mL at 37 °C and transfected with Flag-Tagged plasmids using PEI (Polysciences, 23966-2). After 48 h of transfection ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293FT cells were grown at 1 x 106 cells/mL and transfected with C-terminal FLAG-tagged WT or active-site mutants cloned in pcDNA3.1 using PEI-max (Polysciences #24765-100). Cells were treated with 10 mM sodium butyrate for ∼16 h after transfection and cultured for two additional days ...
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bioRxiv - Biophysics 2021Quote: ... carboxylated 3 µm silica beads (Polysciences) were coated with a mixture of biotinylated pentameric FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs70 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µm polystyrene beads (Polysciences) suspended in 1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: For HIV-1 virus growth assay pNL4-3 and its mutated versions pNL4-3 TC-1G and pNL4-3 TC-3G were transfected into freshly seeded 293T cells using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfection was done with 3 μg polyethylenimine (Polysciences) per 3.5 cm dish 1 day after plating ...
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bioRxiv - Immunology 2021Quote: ... fixed 18 hrs later with 3% formaldehyde (Polysciences) in PBS ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... using polyethylenimine (PEI, 3 μg/ml, Polysciences, 23966), with 1 μg of the indicated plasmids ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Hek293T-cells were transfected with pNL4-3 or pCH058/2960 or pNL4-3 and pMM310 (pBlam-Vpr) via polyethylenimine (Polysciences Inc.)[67] ...
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bioRxiv - Pathology 2019Quote: ... Sections were washed with PBS/5% goat serum 3 times for 3 minutes each and fixed with Aqua-Mount (Polysciences, Warrington, PA). Control kidney sections lacked primary antibodies.
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Polybead Amino Microsphere 3 μm latex beads (Polysciences INC) were labeled with the fluorescent dye DyLight680 mono-N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2020Quote: ... OVA:BSA-coated 3 µm latex beads (Polysciences, Warrington, PA), or OVA-specific MHC-II peptides for 15–30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... BioMag Plus Concanavalin A coated beads (Polysciences: 86057-3) were equilibrated with Binding buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... from Bangs Laboratories and Latex polymer 3-micron beads (17134-15) from Polysciences. Murine IFNγ (315-05 ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...