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Recombinant Human Interleukin 3 Receptor Alpha Low Affinity His tagged
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Creative Biomart Sponsored
Recombinant Human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305), fused with a...Cat# IL3RA-3178H, 20.0 µg, USD $499.0 Ask
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Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of General Virology doi: 10.1099/jgv.0.001231Quote: ... His-tagged recombinant human CD63 protein was obtained from ThermoFisher Scientific Sino Biological (China).The expression and purification of the CD63 LEL domain were performed by the same method used for nsP1a and nsP1a/4 ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Genomics 2017Quote: ... and 100 ng/mL recombinant human interleukin-3 (PeproTech), with a density of 0.1 million cells per milliliter of medium ... -
GE Life Sciences
No products found because this supplier's products are not listed.Cited in Fusion of a functional glutaredoxin to the radical-generating subunit of ribonucleotide reductasebioRxiv - Biochemistry 2018Quote: The recombinant His-tagged protein was first isolated by metal-chelate affinity chromatography using ÄKTA prime system (GE Healthcare): the supernatant was loaded on a HisTrap FF Ni Sepharose column (GE Healthcare) ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in Synthetic Lethality of Wnt Pathway Activation and Asparaginase in Drug-Resistant Acute LeukemiasbioRxiv - Cancer Biology 2018, published in Cancer Cell doi: 10.1016/j.ccell.2019.03.004Quote: ... supplemented with 20% FBS and recombinant human interleukin-3 (R&D systems, Minneapolis, MN), recombinant human interleukin-6 (R&D systems ... -
Qiagen
No products found because this supplier's products are not listed.Cited in CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... His-tagged proteins were purified by Ni-NTA agarose affinity chromatography (Qiagen) and treated with TEV protease to remove the tag ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2017, published in Journal of Experimental Botany doi: 10.1093/jxb/ery005Quote: ... The purification of soluble recombinant his-tagged proteins was done using the TALON Metal Affinity Resin (TAKARA) as described by the supplier ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020, published in eLife doi: 10.7554/eLife.65365Quote: ... Recombinant His-tagged and biotinylated human ACE2 protein (Sino Biological, Cat # 10108-H08H-B) was immobilized on a Streptavidin (SA ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
abcam
No products found because this supplier's products are not listed.Cited in Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypesbioRxiv - Biochemistry 2019, published in eLife doi: 10.7554/eLife.47544Quote: ... anti-alpha integrin alpha 3 #ab190731 (Abcam, Cambridge, MA), anti-Mfg-e8 #MAB2767 (R&D systems ... -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... and recombinant interleukin-2 (20U/ml; Hoffmann-La Roche). Fresh medium containing IL-2 was added twice per week ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... to transfect GFP-tagged human tumor necrosis factor receptor superfamily 10b (GFP-TNFRSF10B/GFP-DR5) plasmid (OriGene) into the cell lines ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017Quote: ... and 5 ng/mL recombinant human interleukin 7 (Miltenyi Biotec). Cells were passaged weekly with vigorous pipetting and filtering through a 70-µm membrane and cocultured again with a fresh monolayer of OP9-DL1 cells ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Zoology 2020, published in PLOS Neglected Tropical Diseases doi: 10.1371/journal.pntd.0009074Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ... -
ImmunoTools
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... recombinant human Interleukin-2 (rh IL-2) (20 ng/mL, ImmunoTools) subsequently referred to as primed stimulation ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... Recombinant human interleukin-1β (IL-1β; 10 ng/mL, 8900; Cell Signaling Technology) was used as a positive control ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... retinoid-related orphan receptor alpha (RORα) and melatonin receptor 1A (MT1) from Santa Cruz Biotechnology (Santa Cruz ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... mouse anti-human estrogen receptor α (Dako), provided at ready-to-use concentration ... -
Promega
No products found because this supplier's products are not listed.Cited in Differential activation of G-protein-mediated signalling by synthetic cannabinoid receptor agonistsbioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... Fc-receptors were blocked using human BD Fc block (BD biosciences). Cells were then labeled with a surface antibody panel which included CD9 ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.Cited in The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019, published in Molecular Microbiology doi: 10.1111/mmi.14370Quote: ... Nitrocellulose membranes were challenged with an anti-His-HRP antibody (Alpha Diagnostics) and a GeneGnome instrument (SynGene ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... interleukin-1 receptor-associated kinase 1 and 4 (IRAK 1/4; IRAK 1/4 inhibitor, 0.1 μM; Tocris); TANK-binding kinase 1 (TBK1 ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: Calu-3 human adenocarcinoma airway epithelial cells (HTB-55™, ATCC®, USA) were cultured in alpha minimum essential medium (αMEM, Corning®, USA) supplemented with fetal bovine serum (10% ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Biophysical Chemistry doi: 10.1016/j.bpc.2019.106193Quote: The activity of recombinant human procaspase-3 and of recombinant human purified caspase-3 (Enzo Life Sciences, Lisboa, Portugal) was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC ... -
Proteintech
No products found because this supplier's products are not listed.Cited in PHAROH lncRNA regulates c-Myc translation in hepatocellular carcinoma via sequestering TIARbioRxiv - Cancer Biology 2021Quote: ... Recombinant human TIAR (Proteintech) was added at indicated concentrations to the probe (∼2 fmol ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0208287Quote: ... recombinant human epidermal growth factor (EGF) (Lonza, CC-4017). Metabolic assay reagents ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019Quote: ... (2) receptors obtained from Illumina sequencing of the transcriptome of the main olfactory epithelium ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
PerkinElmer
No products found because this supplier's products are not listed.Cited in Cannabis sativa Terpenes are Cannabimimetic and Provide Support for the Entourage Effect HypothesisbioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing the human CB1 receptor were obtained from PerkinElmer (#ES-110-C) and utilized for western blots (CB1-CHO-C2 ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ... -
PBL Assay Science
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2017, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1815365116Quote: ... Human interferon alpha 2a (11101-2) was purchased from PBL Assay Science. -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... transforming growth factor beta2 (TGFβ2) 1ng/ml (human-recombinant, Biovision) and fibroblastic growth factor FGF2 5ng/ml (human-recombinant ... -
SignalChem
No products found because this supplier's products are not listed.Cited in Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 proteinbioRxiv - Neuroscience 2017, published in eLife doi: 10.7554/eLife.24779Quote: ... Immunoprecipitated myc-PER2 were incubated with GST-tagged recombinant human SIK3 (aa.1-307) (SignalChem) in kinase assay buffer (50 mM Tris-HCl ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007848Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020, published in Scientific Reports doi: 10.1038/s41598-020-71548-zQuote: ... or recombination activating gene 2 (RAG2) (rat) and interleukin-2 receptor subunit gamma (IL2RG) on the Non-obese Diabetic (NOD) strain (mice) (Jackson Laboratory, Stock No: 005557) and on the Sprague Dawley (SD ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in Glia doi: 10.1002/glia.23792Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in eLife doi: 10.7554/eLife.47172Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ... -
Molecular Devices
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in Nature Communications doi: 10.1038/s41467-019-13244-9Quote: ... and data was collected at 10 kHz sampling and low-pass filtering at 3 kHz (Axon Digidata 1440A and pClamp 10 software; Molecular Devices). -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... All plasmids were propagated in DH5-alpha E.Coli (BIOLINE) and prepared for transfection using a Plasmid Maxi kit (QIAGEN). -
Bethyl
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2020, published in eLife doi: 10.7554/eLife.57659Quote: ... affinity-purified goat anti-human IgG Fc (Bethyl Laboratories, Inc.) and goat anti-rabbit IgG Fc (Abcam ... -
MP Biomedicals
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in Scientific Reports doi: 10.1038/s41598-018-32820-5Quote: ... After embedded in low melting agarose (3%, MP Biomedicals), brains were sectioned at 100 μm thickness with a Leica VT1200S vibratome ... -
Invivogen
No products found because this supplier's products are not listed.Cited in DDX3 is exploited by Arenaviruses to suppress type I interferons and favor their replicationbioRxiv - Microbiology 2017Quote: Human IFN-I bioactivity in tissue culture supernatants was measured with reference to a recombinant human IFN-β standard (InvivoGen) using HEK-BlueTM IFN-α/β cell line (InvivoGen ... -
Eurogentec
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2018, published in Biopolymers and Cell doi: 10.7124/bc.00099EQuote: ... 22-mer oligonucleotide 5′-d[AG3(T2AG3)3]-3′ of human telomeric DNA (Tel22, PDB ID: 143D) from Eurogentec SA (Belgium ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... HRP-tagged secondary antibodies were from Jackson ImmunoResearch; fluorescent Alexa-tagged antibodies were from Molecular Probes.