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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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Recombinant Human CD28 (P10747-1) extracellular domain (Met 1-Pro 152), fused with a...
Cat# CD28-3910H,
50ug , USD $298
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
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No products found
because this supplier's products are not listed.
Emily Nestor Truckenbrod, et al.,
bioRxiv - Immunology 2020
Quote:
... a toll-like receptor 3 agonist (InvivoGen). Peptide doses of 50 ...
-
No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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No products found
because this supplier's products are not listed.
Tao Guo, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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No products found
because this supplier's products are not listed.
Athina Kilpeläinen, et al.,
bioRxiv - Immunology 2021
Quote:
... and Goat anti-human IgA (alpha chain specific) (1/20000) (all from Jackson Immunoresearch). Secondary antibodies were incubated for 30 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
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No products found
because this supplier's products are not listed.
Nicole M. Gaudelli, et al.,
bioRxiv - Genomics 2020
Quote:
... and 250IU/mL of recombinant human interleukin-2 (rhIL-2, CellGenix GmbH). Cells were activated with soluble human anti-CD3 (clone OKT3 ...
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No products found
because this supplier's products are not listed.
Madhukar Vedantham, et al.,
bioRxiv - Immunology 2024
Quote:
... Unspecific binding to low-affinity Fc-receptors was blocked by incubating the cells with unconjugated CD16/32 antibody (BioXCell, clone 2.4G2). Cells were subsequently stained for 30 min at 4 °C with antibodies diluted in the FACS buffer ...
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No products found
because this supplier's products are not listed.
Jianwen Zhou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... GFP-tagged proteins were affinity purified by GFP-trap (ChromoTek) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
His-tagged SSRP1 was PCR amplified from SSRP1 pReceiver (GeneCopoeia) using primers encoding an N-terminal His tag and NdeI/NotI cut sites for cloning into pET22b ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Sabrina Wamp, et al.,
bioRxiv - Microbiology 2021
Quote:
... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
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No products found
because this supplier's products are not listed.
S. Venkatesan, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The NMDA receptor–mediated eEPSCs were analyzed as an average of 3 traces with Clampfit (Molecular Devices) and D-APV (50 μM ...
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No products found
because this supplier's products are not listed.
Xinming Wang, et al.,
bioRxiv - Bioengineering 2021
Quote:
... platelet-derived growth factor receptor alpha (pdgfr-α) (Abclonal), CAPG (ProteinTech) ...
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No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... was combined with 3 μg of recombinant human SQSTM1/p62 protein (GeneTex, GTX68012-pro) in buffer containing 20 mM HEPES (pH 7.5 ...
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No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
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No products found
because this supplier's products are not listed.
Aleksandar Antanasijevic, et al.,
bioRxiv - Immunology 2020
Quote:
... Affinity-purified goat anti-human IgG Fc (Bethyl Laboratories, Inc.) and goat anti-Rabbit IgG Fc (Abcam ...
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No products found
because this supplier's products are not listed.
Shaon Basu, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Ida Lindeman, et al.,
bioRxiv - Immunology 2023
Quote:
... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
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No products found
because this supplier's products are not listed.
Kevin Schmidt, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... PenStrep as well as 1 ng/mL human recombinant basic FGF and 5 ng/mL Insulin (both from Growth Medium 3 SupplementPack, C-39350, PromoCell). HEK293FT and HUVECs were kept in T75 flasks (Sarstedt ...
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No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
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No products found
because this supplier's products are not listed.
Tommy Stormberg, Yuri L. Lyubchenko,
bioRxiv - Molecular Biology 2022
Quote:
... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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No products found
because this supplier's products are not listed.
Jenifer P. Suntharalingham, et al.,
bioRxiv - Genetics 2024
Quote:
... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
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No products found
because this supplier's products are not listed.
Samantha R. Mackin, et al.,
bioRxiv - Microbiology 2022
Quote:
... The antigen-coupled microspheres were washed and incubated with heat-inactivated serum samples at an appropriate sample dilution (1:100-1:400 for antibody isotyping and 1:1,000 for all low-affinity FcγRs) overnight in 384-well plates with continuous shaking (Greiner Bio-One). Unbound antibodies were washed away using the magnetic 384-well HydroSpeed Plate Washer (Tecan ...
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No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
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No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were pre-incubated with 3 nM of CypHer5E-tagged anti-VGAT antibodies (131 103CpH, Synaptic Systems, Germany) in the culture medium and incubated in the 5% CO2 incubator for 2 h at 37°C to specifically label inhibitory synaptic vesicles in presynaptic terminals ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
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Cat# HY-P70248-10 μg,
10 μg, USD $170.0
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Dong Yan Zhang, et al.,
bioRxiv - Neuroscience 2024
Quote:
... To determine the binding affinity between APOE-lipoprotein and its receptor LDLR (MedChemExpress), we first labeled APOE3 and APOE4 with RED-NHS 2nd generation ...
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No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2021
Quote:
To investigate Fc receptor binding recombinant Fc receptors with an AviTag were biotinylated using a Bir500 kit (Avidity, Aurora, CO, USA) according to manufacturer’s instructions and purified using a Zeba Spin Desalting Column ...
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No products found
because this supplier's products are not listed.
Arnaud N’Guessan, et al.,
bioRxiv - Genomics 2022
Quote:
... and goat anti-human IgA (alpha chain) DyLight800 (Rockland Immunochemicals) antibodies at a final concentration of 0.1 μg/ml and 1 μg/ml ...
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No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
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No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
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No products found
because this supplier's products are not listed.
Michael J Abrams, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and 0.1 mg/mL insulin (human recombinant, MP Biomedicals 0219390080). To introduce the molecular factors ...
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No products found
because this supplier's products are not listed.
J. Drube, et al.,
bioRxiv - Cell Biology 2021
Quote:
... human complement 5a receptor 1 (C5aR1) with C5aR-agonist (AnaSpec AS65121, in measuring buffer), human muscarinic 1,2,3,4 ...
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No products found
because this supplier's products are not listed.
Filippo Vascon, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Lucas M. Demey, Ritam Sinha, Victor J. DiRita,
bioRxiv - Microbiology 2022
Quote:
... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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No products found
because this supplier's products are not listed.
Saurabh Joshi, et al.,
bioRxiv - Plant Biology 2021
Quote:
... low (IBIDI GmbH) imaging dish containing KPBS buffer and images were captured using Leica DMI6000b epifluorescence inverted microscope equipped with GFP filter cube ...
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No products found
because this supplier's products are not listed.
Eliram Nof, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human airway epithelial cell line Calu-3 (American Type Culture Collection, Manassas, VA) was used in this study (passage number 15-20) ...
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No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...