Elizabeth V. K. Ledger, Andrew M. Edwards,bioRxiv - Microbiology 2023
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... or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch). Antibody incubations were carried out statically for 1 h at room temperature in the dark ...
Mark A. Skylar-Scott, et al.,bioRxiv - Bioengineering 2020
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Wild type PGP1-iPSCs were dissociated using EDR and seeded into anti-adhesive treated V-bottom 96-well plates (Globe Scientific, #120130) at a density of 1,000 cells/96-well well in EBCM + 10 μM Y-27632 for 12 h to generate organoid cores.
Abdoulie O. Touray, et al.,bioRxiv - Microbiology 2023
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... ChIP was performed with a total of 15 µg of sonicated chromatin and 10 µg of Mab anti-V5 (BioShop Canada Inc.). The antibodies were cross-linked to Protein G Mag Sepharose Xtra (GE Healthcare ...
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... LaG-16 anti-GFP nanobodies32 were recombinantly produced in house and labeled with α-Biotin-ω-(succinimidyl propionate) 24(ethylene glycol) (Iris Biotech) as previously described33,34 ...
Beatriz A. Osuna, et al.,bioRxiv - Microbiology 2019
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The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
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Calpain-2 immunostaining was performed using a rabbit anti-human calpain-2 (10 µg/ml, catalog No. RP-3 Calpain-2; Triple Point biologics, Forest Grove, OR). CD68 immunostaining was performed using a rabbit anti-human CD68 antibody (1:200 ...