-
No products found
because this supplier's products are not listed.
Eric Hee Jun Lee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Membranes were then blocked for 1 hour at room temperature in blocking buffer containing 5% PhosphoBLOCKER blocking reagent (Cell Biolabs) in TBST ...
-
No products found
because this supplier's products are not listed.
Leandro de Souza Silva, et al.,
bioRxiv - Immunology 2023
Quote:
... glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2 ...
-
No products found
because this supplier's products are not listed.
Karina Kinghorn, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Membranes were blocked for 1 hr with OneBlock Western-CL Blocking Buffer (Genesee), and incubated with primary antibodies diluted in OneBlock (Supplementary Table S8 ...
-
No products found
because this supplier's products are not listed.
Sudha Silwal Gautam, et al.,
bioRxiv - Bioengineering 2020
Quote:
... calcitonin gene-related peptide (CRGP, 1:800, guinea pig polyclonal; Progen Biotechnik GmbH), or vascular endothelial cell marker von Willebrand factor (vWF ...
-
No products found
because this supplier's products are not listed.
Elad Horwitz, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... A custom library of kinase inhibitors was purchased from TargetMol. Cell counts for long term viability assays were carried using CellDrop (Denovix ...
-
No products found
because this supplier's products are not listed.
Logan R.J. Bailey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Primary antibody was applied overnight at 4°C in blocking buffer (α-Actinin, Sigma A7811, 1:2500; AURKB, Sigma A5102, 1:1000; GADD45A, CST 4632, 1:1000; GAPDH, Fitzgerald 10R-2932 ...
-
No products found
because this supplier's products are not listed.
Kailash Venkatraman, et al.,
bioRxiv - Biophysics 2023
Quote:
... Cells were back-diluted 1:100 in fresh CSM containing 2% glucose or 3% glycerol and shaken in a plate reader (Tecan) for 48 hours ...
-
No products found
because this supplier's products are not listed.
Vinko Besic, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Rabbit polyclonal antibodies (PABPC1, Atlas Antibodies #HPA045423; p70 S6 kinase α (H-160), Santa Cruz #sc-9027 ...
-
No products found
because this supplier's products are not listed.
Nikolai N. Sluchanko, et al.,
bioRxiv - Biochemistry 2023
Quote:
Crystals were briefly soaked in cryo-solution containing 20% glycerol (Hampton Research, USA) immediately prior to diffraction data collection and flash-frozen in liquid nitrogen ...
-
No products found
because this supplier's products are not listed.
Carlos J. Díaz Osterman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Lentiviral vectors for GFP-FAK wildtype and FAK kinase-dead (R454) in pCDH-CMV-MCS-Puro (System Biosciences) were used as described (Chen et al. ...
-
No products found
because this supplier's products are not listed.
Nithya Gajendran, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... After blocking the membranes with 5% blotto (G-Biosciences Blot-Quikblocker # 786-011) in phosphate-buffered saline containing 0.1% (v/v ...
-
No products found
because this supplier's products are not listed.
I.R. Akberdin, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Another target of calmodulin is Ca2+/calmodulin-dependent protein kinase kinase 2 (CAMKK2) that phosphorylates AMPK Thr172 thereby activating the kinase (Abbott et al., 2009). In turn ...
-
No products found
because this supplier's products are not listed.
Yanzhuo Kong, et al.,
bioRxiv - Microbiology 2022
Quote:
... and glycerol content was measured using Megazyme glycerol assay kit (Megazyme, Wicklow, Ireland).
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
A peptide (EpiGentek) containing the SARS-CoV-2-specific furin motif and tagged with His and biotin at the N- and C-terminals ...
-
No products found
because this supplier's products are not listed.
Jing Zeng, et al.,
bioRxiv - Genetics 2023
Quote:
... and 100 ng ml-1 Preclinical FMS-like Tyrosine Kinase 3 Ligand (FLT3L) (CellGenix, cat# 1415-050). HSPCs were electroporated with 3xNLS-SpCas9:sgRNA or 3xNLS-HiFi- SpCas9:sgRNA RNP immediately ...
-
No products found
because this supplier's products are not listed.
Emily K. Sims, et al.,
bioRxiv - Pathology 2019
Quote:
... Three of the donors with type 1 diabetes had detectable random serum C-peptide and 13 were classified by nPOD as C-peptide negative (random serum C-peptide <0.017nmol/L via TOSOH immunossay) (12) ...
-
No products found
because this supplier's products are not listed.
Sung Hee Jo, et al.,
bioRxiv - Plant Biology 2019
Quote:
... 1 μM flg22 peptide (Cat No: FLG22-P-1; Alpha Diagnostics, Inc., San Antonio, TX, USA) was used as a positive control (Bethke et al. ...
-
No products found
because this supplier's products are not listed.
Yong Zou, et al.,
bioRxiv - Plant Biology 2023
Quote:
... peptide grade), diisopropylcarbodiimide (DICI, peptide grade), piperidine (PIP, peptide grade), and trifluoroacetic acid (TFA, purity 99%) were all from Iris Biotech, GmbH ...
-
No products found
because this supplier's products are not listed.
Elsio Wunder Jr., et al.,
bioRxiv - Microbiology 2020
Quote:
... the arrays were probed at 1/100 dilution in protein array blocking buffer (GVS) supplemented with E ...
-
No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
-
No products found
because this supplier's products are not listed.
Martijn R. Molenaar, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Human LRAT peptide (AA 1-39) was synthesized by Bio-Synthesis (Lewisville, Texas, USA).
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The in vitro kinase assay (HTRF KinEASE-STK S1, CisBio 62ST1PEB) was optimized to the linear reaction range of the enzymes Lats1 (Carna 01 −123 ...
-
No products found
because this supplier's products are not listed.
James E. Keener, et al.,
bioRxiv - Biophysics 2022
Quote:
... nanodiscs were gently mixed 19:1 v/v with neat glycerol carbonate at >90% purity (Tokyo Chemical Industry Co., Inc.).17–18 The mixture was incubated for several minutes at room temperature prior to native MS.
-
No products found
because this supplier's products are not listed.
Katrin Schrenk-Siemens, et al.,
bioRxiv - Neuroscience 2019
Quote:
... before blocking solution (10% goat serum (PAN Biotech) in PBS ...
-
No products found
because this supplier's products are not listed.
Amanda Janesick, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... then incubated in a blocking buffer (ScyTek AAA999) for 30 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Giulio Giuliani, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Unspecific sticky sites were blocked by a blocking solution composed of 0.1% Triton X-100 1% glycine (SERVA 23390.02) 10% goat serum (Euroclone ECS0200D) in PBS for 1 hour at RT ...
-
No products found
because this supplier's products are not listed.
K. Joannou, et al.,
bioRxiv - Immunology 2021
Quote:
... Blocking steps were done with Normal Mouse Serum (Cedarlane: CLS303) and/or Normal Rat Serum (STEMCELL Technologies ...
-
No products found
because this supplier's products are not listed.
Ana Maia, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Membranes were blocked with Rockland blocking buffer (Biomol GmbH, Hamburg, Germany) for 1 hour at RT and incubated with the primary antibodies (Supplementary Table 6 ...
-
No products found
because this supplier's products are not listed.
Daniel J. Boesch, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 5% (w/v) glycerol was applied to a Quantifoil 300-mesh R1.2/1.3 grid (Quantifoil, Micro Tools GmbH, Germany), which had been pre-treated with glow discharge using Pelco EasiGlow immediately before use ...
-
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...
Cat# LS004194,
100 mg, $42.00
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Matthias Felten, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Proteolysis into peptides was accomplished using 0.5% (w/w) Trypsin (sequencing grade; Worthington Biochemical) (37 °C ...
-
No products found
because this supplier's products are not listed.
Jennifer E. Wu, et al.,
bioRxiv - Immunology 2023
Quote:
... P14 transgenic mice expressing a TCR specific for the LCMV peptide DbGP33-41 were bred in-house and backcrossed onto the C57BL/6 background (Charles River); recipient WT C57BL/6 mice were purchased from Charles River ...
-
No products found
because this supplier's products are not listed.
Ting-Yu Lin, et al.,
bioRxiv - Physiology 2022
Quote:
... Recombinant kinase WEE1 was purchased from BPS Biosciences. Recombinant PIK3CG was purchased from Thermo Fisher.
-
No products found
because this supplier's products are not listed.
Hanyu Pan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Yang.17 The PD-1 blocking scFv E27 gene was synthesized by GENEWIZ (GENEWIZ, Suzhou, China) according to the previous report.36 3BNC117 CAR and E27 were cloned into a lentiviral vector (pTRPE-GFP-T2A-mRFP ...
-
No products found
because this supplier's products are not listed.
Sherif Salah, et al.,
bioRxiv - Immunology 2022
Quote:
... and membrane protein peptide (by AMSBIO) were procured ...
-
No products found
because this supplier's products are not listed.
Simona Giunta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Incubations with primary antibodies were conducted in blocking buffer for 1 h at room temperature using the following antibodies: ACA (1:500; 15-235-0001, Antibodies Incorporated), ATR (1:1000 ...
-
No products found
because this supplier's products are not listed.
Ji-Hoon Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... incubated with primary antibody against spike protein diluted 1:5,000 in blocking buffer (Elabscience, E-AB-V1006) overnight in a 37°C incubator with 5% CO2 injection ...
-
96 well glass bottom plate. Black polystyrene frame with #1 glass(0.13-0.16mm), with lid,...
Cat# P96-1-N,
20/case, $226.00
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Aleksandra Marconi, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The specimens were positioned in 100% glycerol in glass-bottomed dishes (Cellvis) and imaged using a Leica M165FC with Leica DFC7000T camera ...
-
No products found
because this supplier's products are not listed.
Francisco Santos, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and blocking with 5% goat serum (Abbkine Scientific) and 0.3% Triton X-100 in PBS1x for 1h at RT with agitation ...
-
No products found
because this supplier's products are not listed.
Boram Lee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The fluorescent peptide–dextramers were synthesized by Immudex, Copenhagen ...
-
No products found
because this supplier's products are not listed.
Nikolas Nikolaou, et al.,
bioRxiv - Neuroscience 2020
Quote:
... This was removed and coverslips were incubated with fresh blocking solution containing anti-GFP 1:1000 (Torrey Pines Biolabs-TP401) and anti-SNRNP70 1:200 (Sigma-AV40276 ...
-
No products found
because this supplier's products are not listed.
Eric E. Gardner, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 0.05% Triton X-100 in 1X PBS pH 7.2 supplemented with mouse-on-mouse blocking reagent (Vector Biolabs; 1:50) and Fc receptor blocker reagent (Innovex Biosciences ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... antibodies blocking the type I IFN receptor (mouse anti-human IFNAR2 antibody, clone MMHAR-2, PBL Assay Science Cat#21385-1) or isotype control (Ultra-LEAF Purified mouse IgG2a ...
-
No products found
because this supplier's products are not listed.
Chamseddine Ben Brahim, et al.,
bioRxiv - Cell Biology 2020
Quote:
... arrays were incubated 30 min Super G blocking buffer (Grace Biolabs), rinsed in water ...
-
No products found
Pooja Khanna, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Membranes were blocked with Advanblock blocking buffer (Advansta R-03726-E10) for 1 hour at room temperature and incubated at 4°C in primary antibody overnight ...
-
No products found
because this supplier's products are not listed.
Francesca Boscolo Sesillo, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Slides stained for collagen I were first washed and then incubated in in blocking buffer (10% goat serum, 0.3% Triton x-100, 1% BSA (Gemini Bio-Products, 700-100P) in PBS ...
-
No products found
because this supplier's products are not listed.
Douek-Maba Orit, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... followed by an overnight incubation at 4°C in blocking solution with anti phospho-histone 3 (PhH3) antibody or anti-caspase 3 (Cas3) antibody (both 1:300; Lifespan Bioscience. Washington US). Next ...
-
The CY3 (Red) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically...
Cat# 5276-60UG,
0.3 mg, USD $290.0
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Amber L. Altrieth, et al.,
bioRxiv - Cell Biology 2023
Quote:
Collagen hybridizing peptide (CHP) conjugated to 5-carboxyfluorescein (5-FAM) (Advanced BioMatrix) was solubilized per manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Hugo Belda, et al.,
bioRxiv - Microbiology 2024
Quote:
... Kinase activity was assessed after addition of 12µl Kinase Detection reagent by measuring luminescence on a multi-mode microplate reader FLUOstar Omega (BMG Labtech). Data were analysed using GraphPad Prism version 10 and IC50s were calculated from a four-parameters logistical fit of the data.
-
No products found
because this supplier's products are not listed.
Thomas J. Cahill, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... The hearts were then orientated according to dorsal-ventral surface aspect and mounted in 50% glycerol/PBS in glass-bottomed dishes (Mattek). Imaging was performed using an Olympus FV 1000 or Zeiss LSM 780 scanning confocal microscope ...
-
No products found
because this supplier's products are not listed.
Bijal A. Parikh, et al.,
bioRxiv - Immunology 2019
Quote:
... Fc receptor blocking was performed with 2.4G2 (anti-FcγRII/III) hybridoma (American Type Culture Collection) culture supernatants ...