-
No products found
because this supplier's products are not listed.
Patryk Slusarczyk, et al.,
bioRxiv - Physiology 2022
Quote:
... Ferritin Light Chain (FTL, Abcam, ab69090) and HO-1 polyclonal antibody (ENZO ...
-
No products found
because this supplier's products are not listed.
Edward B. Irvine, et al.,
bioRxiv - Immunology 2022
Quote:
... then 80µL of secondary anti-human Igκ light chain HRP-conjugated antibody (ThermoFisher, A18853) diluted 1:1000 in 0.1% BSA-PBS was added ...
-
No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Goat anti-Human Lambda Light Chain Antibody HRP Conjugated (Bethyl Laboratories) at 1:10000 ...
-
No products found
because this supplier's products are not listed.
Jingwei Zeng, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... anti-mouse light chain specific (Millipore AP200P), anti-rabbit (Fisher 31462) ...
-
No products found
because this supplier's products are not listed.
Yuta Ishizuka, et al.,
bioRxiv - Neuroscience 2022
Quote:
... light chain specific (Jackson ImmunoResearch Labs ...
-
No products found
because this supplier's products are not listed.
Camille N. Pierre, et al.,
bioRxiv - Immunology 2023
Quote:
... Heavy chain and light chain plasmids were obtained from GenScript. Expi293 cells (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Sung Hoon Lee, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Myosin Light Chain 2 (Cell Signaling, 8505S), mDia1 (Cell Signaling ...
-
No products found
because this supplier's products are not listed.
Jared Feldman, et al.,
bioRxiv - Immunology 2021
Quote:
... and kappa light chain (PE anti-human kappa light chain; BD Biosciences) double positivity using a SH800S Cell Sorter (Sony Biotechnology) ...
-
No products found
because this supplier's products are not listed.
Israa Al Jamal, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
-
No products found
because this supplier's products are not listed.
Eduardo Olmedillas, et al.,
bioRxiv - Microbiology 2023
Quote:
Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
-
No products found
because this supplier's products are not listed.
Antonia Sophia Peter, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-mouse Ig light chain lambda APC # 407306 and anti-mouse IgG light chain kappa PE both from Biolegend, San Diego ...
-
No products found
because this supplier's products are not listed.
Quynh T. Phan, et al.,
bioRxiv - Microbiology 2021
Quote:
... anti-light chain antibody (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Leire de Campos-Mata, et al.,
bioRxiv - Immunology 2023
Quote:
... Amplicon from the first PCR reaction were used as templates for cloning into antibody expression vectors (Abvec2.0-IGHG1 for the heavy chain and Abvec1.1-IGKC or Abvec1.1.-IGLC2-XhoI for of the light chains, all from AddGene).
-
No products found
because this supplier's products are not listed.
Ioanna Tzani, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
-
No products found
because this supplier's products are not listed.
Teha Kim, et al.,
bioRxiv - Immunology 2023
Quote:
... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
-
No products found
because this supplier's products are not listed.
Lin Pan, et al.,
bioRxiv - Neuroscience 2022
Quote:
... BoNT-B Light Chain (R&D Systems, cat #AF5420-SP, dilution 1:1000), VAMP2 (Synaptic Systems ...
-
No products found
because this supplier's products are not listed.
Xiaozheng Xu, et al.,
bioRxiv - Immunology 2021
Quote:
... human PD-L1-His (Sino Biological, #10084-H08H), human ICAM-1-His (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Harry B. Gristick, et al.,
bioRxiv - Immunology 2022
Quote:
... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
-
No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and Lambda light chains (Catalog #1060-01, Southern Biotech). After overnight incubation at 4□°C ...
-
No products found
because this supplier's products are not listed.
Paritosh Parashar, et al.,
bioRxiv - Immunology 2022
Quote:
... Samples showing the correct size band for heavy and light chain amplification in the respective wells were subsequently cloned into pAb20-hCHIgG1 (Synbio; for heavy chain) and pAb20-hCK (Synbio; for light chains) through in-fusion cloning (Takara). The ligated product was transformed to DH5-α competent cells ...
-
No products found
because this supplier's products are not listed.
Karina Flores Montero, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Purification of His6-light chain TeTx was done using Ni-NTA resin (Qiagen) in 50 mM Tris HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Sedat Odabas, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Quantitative Real-Time polymerase chain reaction (qRT-PCR; Light-Cycler 480 instrument Roche Diagnostics, Mannheim, Germany) analysis was performed for Collagen Type I (COL1A1) ...
-
No products found
because this supplier's products are not listed.
Padriac Hooper, et al.,
bioRxiv - Biophysics 2024
Quote:
... Ferritin (F4503, Merck & Co.) concentrations were 3.76 to 10.21 µM ...
-
No products found
because this supplier's products are not listed.
Qiujie Wang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-FTL (10727-1-AP) was from Proteintech (Shanghai, China), and anti-GSTZ1 (GTX106109 ...
-
No products found
because this supplier's products are not listed.
Dora Mahecic, et al.,
bioRxiv - Biophysics 2020
Quote:
... The excitation was then imaged onto the sample using a tube lens (fTL = 350 mm, 49-289-INK, Edmund Optics) and an objective lens (APON60XOTIRF, Olympus), resulting in a final magnification of ∼116x ...
-
No products found
because this supplier's products are not listed.
Stefano R. Bonissone, et al.,
bioRxiv - Immunology 2020
Quote:
... Gel bands for heavy and light chains were excised and in-gel digested using trypsin (Promega), chymotrypsin (Promega) ...
-
No products found
because this supplier's products are not listed.
Torben Schiffner, et al.,
bioRxiv - Immunology 2024
Quote:
Amplified IgG heavy- and light-chain variable regions were sequenced on an Illumina MiSeq (600-base v3 kit; Illumina) and reads corresponding to the same plate/well location were combined into consensus sequences ...
-
No products found
because this supplier's products are not listed.
Zachary M. Earley, et al.,
bioRxiv - Immunology 2022
Quote:
... beads selectively bound to IgA through kappa light chain were separated from the supernatant with EasySep magnetic stand (StemCell technologies). Supernatant was discarded and beads washed 3 times ...
-
No products found
because this supplier's products are not listed.
Pragya D Yadav, et al.,
bioRxiv - Microbiology 2021
Quote:
... encoding the light and heavy chain genes of ZRC3308-A7 and ZRC3308-B10 were used for transfection in CHOK1SVGSKO (Lonza, Switzerland) cell line by electroporation following pre-optimized parameters using Neon transfection system (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Sachin Yadav, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the cells were labeled with a rat anti-CPM antibody (in-house) and microbead-conjugated anti-rat immunoglobulin kappa light chain antibody (130-047-401, Miltenyi Biotec). This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Pei-Shan Sung, et al.,
bioRxiv - Immunology 2022
Quote:
... and images were captured by a light microscope with polarized light (Nikon). Quantification of collagen was performed by MetaMorphTM ...
-
No products found
because this supplier's products are not listed.
Jeremias N. Brand, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... using light microscopes (Leica DM2500 ...
-
No products found
because this supplier's products are not listed.
Awatef Allouch, et al.,
bioRxiv - Immunology 2022
Quote:
... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
-
No products found
because this supplier's products are not listed.
Hsiao-Fan Lo, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... light chain specific (Genetex, GTX221666; Jackson Lab, 211-032-171), which do not detect IgG heavy chain.
-
No products found
because this supplier's products are not listed.
Ying Dong, et al.,
bioRxiv - Immunology 2022
Quote:
... anti-mouse lambda light chain (Novus Biologicals, Catalog number NB7552), anti-FLAG (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
-
No products found
because this supplier's products are not listed.
Zachary R. Crook, et al.,
bioRxiv - Bioengineering 2024
Quote:
... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
-
No products found
because this supplier's products are not listed.
Francesco Vallania, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Cells were frozen in 10% DMSO/10% heat inactivated (HI) human AB serum (Corning) for later flow cytometry staining ...
-
No products found
because this supplier's products are not listed.
Felix J. Flomm, et al.,
bioRxiv - Microbiology 2022
Quote:
Lattice light-sheet microscopy was performed on a Zeiss Lattice Light Sheet 7 (Carl Zeiss) as part of an early adaptor program ...
-
No products found
because this supplier's products are not listed.
Gabriele Cerutti, et al.,
bioRxiv - Biochemistry 2021
Quote:
... by co-transfection heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37 °C degree shaker at 125RPM and 8% CO2.
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Theodora A. Constantin, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Light emission was measured using a VICTOR Light luminometer (PerkinElmer). For LNCaP/Luc lysates ...
-
No products found
because this supplier's products are not listed.
Ali Akbar Karkhaneh Yousefi, et al.,
bioRxiv - Biophysics 2023
Quote:
... using 10% Human Fn (Human Fn, Promocell) in PBS ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Xin Su, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 100μg of anti-Rat-kappa light chain (MAR18.5, BE0122; BioXCell) on day 1 ...
-
No products found
because this supplier's products are not listed.
Qian Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
-
No products found
because this supplier's products are not listed.
Hyung-Woo Jeon, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Light properties were measured by LI-250A light meter (LI-COR) equipped with LI-190R quantum sensor (LI-COR ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
-
No products found
because this supplier's products are not listed.
Jürgen Graf, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Emission light was separated from excitation light using a 670-nm dichroic mirror (670 DCXXR, Chroma Technology), short-pass filtered at 680 nm and detected by a photomultiplier tube (12 bit ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...