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Citations for Millipore Sigma :
1 - 50 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... anti-mouse light chain specific (Millipore AP200P), anti-rabbit (Fisher 31462) ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit light chain specific (Millipore MAB201P) and anti-goat (Invitrogen A27014 ...
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bioRxiv - Biochemistry 2021Quote: ... ferritin (Sigma): R2 was 0.994 ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Bound antibodies were detected with Peroxidase conjugated goat anti-human kappa light chains antibody (A7164-1ML, Sigma). 100 ul TMB(Tetramethylbenzidine,Biopanda TMB-S-001 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Cell Biology 2023Quote: ... The final concentration of ferritin (horse spleen ferritin, type I, Sigma) was 50 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... ferritin (equine spleen, Sigma), and ferrous (iron (II ...
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bioRxiv - Cell Biology 2023Quote: ... cationized ferritin (Sigma-Aldrich) was added in the saline solution at 0.25 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Biophysics 2021Quote: A ferritin-agar phantom was prepared by adding horse spleen ferritin (Sigma, ref. F4503) to a 1.5% agar solution ...
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bioRxiv - Bioengineering 2020Quote: ... horse spleen ferritin nanocages (Sigma), or adeno-associated virus (empty capsids ...
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bioRxiv - Biochemistry 2021Quote: ... Equine Spleen Ferritin (Sigma F4503) without further purification was dissolved in buffer (3.5mM NH4SO4 and 100mMTris at pH7.4) ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 10 nM recombinant TeNT–light chain (Sigma–Aldrich) for 16 h in a CO2 incubator ...
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bioRxiv - Pathology 2020Quote: The light chain 3-phosphatidylethanolamine-conjugate (LC3)-II (Sigma Aldrich, 1:3000) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2019Quote: ... horse spleen ferritin (Sigma-Aldrich, US) was deposited on a thin carbon film (∼5 nm ...
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bioRxiv - Systems Biology 2022Quote: ... Ferritin (F4503) was from Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... the myosin light chain kinase (MLCK) inhibitor ML-7 (10 μM, EMD Millipore), or the Ca2+ inhibitor 2APB (10 μM ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced in 50 ml of 0.8×106 HEK293-6E cells by co-transfection of heavy chain (IgG1) and light chain (Igλ, Igκ) antibody expression plasmids with polyethylenimine (PEI; Sigma-Aldrich). Cells were maintained at 37°C and 5% CO2 in FreeStyle 293 Expression Medium (Thermo-Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
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bioRxiv - Biochemistry 2021Quote: Crystals of ferritin (Sigma F4503 Lot# SLBV4372) were obtained using the microbatch method under oil[21] at room temperature (293 K) ...
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bioRxiv - Microbiology 2020Quote: ... anti-human IgM (μ-chain specific, Sigma, St. Louis, MO) was covalently coupled to NHS HP SpinTrap (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Immunology 2020Quote: ... horse spleen ferritin (1mg/ml; Sigma, Gillingham, UK) was orally administered to aged mice via drinking water on days 0–2 and 7–9 ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... 1:3000 dilution of anti-human IgA (α-chain-specific) (Sigma) or 1:3000 dilution of anti-human IgM (μ-chain-specific ...
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bioRxiv - Immunology 2021Quote: ... or 1:3000 dilution of anti-human IgM (μ-chain-specific) (Sigma) antibody subtypes and 1:6000 dilution of anti-human IgG1 ...
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bioRxiv - Physiology 2021Quote: ... ferritin (iron) from equine spleen – Type I standard (Sigma Aldrich) was diluted to 100 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: 49 mg of cationized ferritin from horse spleen (Sigma-Aldrich F1897) was sonicated for 10-15 seconds and then added to three million DG-75 cells for each condition ...
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bioRxiv - Neuroscience 2022Quote: ... Ferritin antibody was used to stain microglia 34 (1/500, Sigma) and anti-GFAP (1/100 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids encoding heavy and light chains were then co-transfected into the 293T cell line by the polyethylenimine method (408727; Sigma), and antibody were harvested for further study.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cloned into the mammalian cell expression vector as described previously.[50] Both the heavy and light chain constructs were co-transfected into Expi293 cell lines using transfection reagent PEI (Sigma). After 7 days of expression ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with primary antibodies against ferritin (1:3000, Sigma F5012) and PLP (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Ref-erence samples such as horse spleen ferritin (HoSF, (Sigma-Aldrich, F7879), magnetosomes ...
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bioRxiv - Biochemistry 2021Quote: ... 250 µg of B6 heavy and 250 µg of B6 light chain encoding plasmids were co-transfected per liter of suspended HEK293F culture using 293Free transfection reagent (Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected at a density of 3 x 106 with equal amounts of heavy and light chain paired plasmids using polyethyleneimine (PEI, Sigma-Aldrich) at a ratio of 1:3 of total amount of plasmid to PEI ...
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bioRxiv - Immunology 2022Quote: Monoclonal antibody production was performed using 293-6E cells (National Research Council of Canada) by co-transfection of heavy and light chain expression plasmids using 25 kDa branched polyethylenimine (Sigma-Aldrich). Culture supernatants were harvested after an incubation period of 6-7 days at 37°C and 6% CO2 under constant shaking in FreeStyle Expression Medium supplemented with penicillin (20 U/ml ...
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:400 mouse anti-light chain clathrin antibody (Sigma Aldrich, C1985) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...