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Citations for Thermo Fisher :
1 - 50 of 6619 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... then 80µL of secondary anti-human Igκ light chain HRP-conjugated antibody (ThermoFisher, A18853) diluted 1:1000 in 0.1% BSA-PBS was added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5-μm frozen sections of uterine myometrium embedded in OCT compound were obtained using Leica CM1510 S cryostat and immunostained for phosphorylated myosin light chain kinase (1:200 rabbit anti-mouse phosphomyosin light chain kinase, Invitrogen) and imaged with the Zeiss Axioskop 40 microscope ...
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bioRxiv - Cell Biology 2022Quote: ... anti-mouse-Clathrin light chain (Invitrogen, 1:2000), anti-mouse-Dynamin2 (Santa Cruz) ...
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...
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bioRxiv - Cell Biology 2021Quote: ... with heavy chain and light chain plasmids using an ExpiCHO™ Expression System (Gibco). Cell culture was harvested after an 8-to 14-day incubation at 37C with humidified atmosphere of 8% CO2 with shaking ...
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bioRxiv - Cell Biology 2022Quote: ... anti-FTL (MA5-32755; Thermo Fisher Scientific), anti-NCOA4 (SAB1409837 ...
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bioRxiv - Immunology 2023Quote: ... 20 µg of Heavy chain and Light chain plasmid was diluted in 1mL OptiMEM(Gibco) and 120 µL polyethyleneimine (1 mg/mL PEI ...
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bioRxiv - Biochemistry 2020Quote: ... Captureselect Biot A-light chain κ HU and light chain λ HU antibodies and Myone Streptavidin T1 beads were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Antibody heavy chain and light chain constructs were transiently expressed with the Expi293 Expression System (ThermoFisher). HC and LC plasmids were cotransfected at a 1:2.5 ratio with transfection reagent FectoPRO (Polyplus ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... and human H-chain ferritin (HHF) (UniProt: P02794) were custom synthesized by Integrated DNA Technologies and cloned into pcDNA3.1 (+) Zeocin (Invitrogen) using restriction cloning or Gibson assembly ...
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bioRxiv - Cell Biology 2021Quote: ... Clathrin light chain (Invitrogen, Cat#: MA5-11860, 1:200 dilution) and Halotag (Promega ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Microbiology 2021Quote: ... plasmids of paired heavy chain (IgA1, IgA2) and kappa light chain together with a J chain were co-transfected into Expi293™ expression system (Thermo Fisher Scientific) at the ratio of 1:1:1 following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... vectors containing an Ig light chain gene and the pcDNA4 (Invitrogen) vectors containing an Ig heavy chain gene were simultaneously transfected into Expi293 cells using Expi293 Expression System Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... synthetic genes for light and heavy chains were obtained from Invitrogen GeneArt ...
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Four hundred micrograms of heavy chain and light chain plasmid were complexed with Expifectamine (ThermoFisher Cat No. A14526) and added to the Expi293 cells ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Immunology 2022Quote: ... 709 and FenAB136 light chain were synthesized commercially (Thermo Fisher Scientific, GeneArt), while the VDJ regions of FenAB208 were cloned out of the IgG vector templates ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Immunology 2022Quote: Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Immunology 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently expressed in Expi-CHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently transfected ExpiCHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2022Quote: Fab generation was initiated with plasmids encoding the truncated heavy chain and light chain of LY-CoV1404 (1 ug/mL each) that were co-transformed into expi293 cells (expifectamine, Gibco) for protein expression (5 days) ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ExpiCHO cells were transfected with equimolar amounts of pLM-M28 light chain and pLM-M28 heavy chain expressing plasmids according to the manufacturer’s protocol (Thermo Fisher). At 12 days post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Bioengineering 2024Quote: ... c-myc epitope tag and the variable heavy chain from mAbCII linked to the variable light mAbCII chain was procured from GeneArt (ThermoFisher). This fragment was cloned into a lentiviral expression plasmid we have previously described (31 ...
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bioRxiv - Immunology 2024Quote: ... The resulting human chimeric heavy and light chain genes were separately cloned in pJET 1.2/blunt vector ( Thermo Fisher Scientific, Waltham, MA, USA). The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding heavy and light chains were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and clone 2 hole light chain plasmid) with ExpiFectamine 293 transfection kit (Thermofisher). The expression and antibody purification protocol was similar to recombinant antibody expression described as above ...
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bioRxiv - Immunology 2021Quote: ... IgGs and 6xHis-tagged Fabs were expressed by transient transfection of paired heavy chain and light chain expression plasmids into HEK293-6E or Expi293 cells (Life Technologies). Fabs and IgGs were purified from transfected cell supernatants using Ni-NTA (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... a heavy chain (HC) and light chain (LC) plasmid was generated for co-expression in HEK293 suspension culture cells (Expi293F cells) (Fisher Scientific). For species specificity swapping experiments ...
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bioRxiv - Biophysics 2022Quote: A 250 mL culture of Expi293 cells at 3e6 cells/mL was transfected with 100 μg each of the heavy chain and light chain plasmids (pMAS478, pMAS481) in 20 mL of Opti-MEM (Life Technologies) supplemented with 200 μL of FectoPRO (Polyplus) ...
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bioRxiv - Immunology 2024Quote: Antibody heavy chain (HC) and light chain (LC) sequence pairs generated from 10X Genomics protocols were synthesized (Geneart, Thermo Fisher Scientific) and cloned into expression vectors containing an IgG1 HC or LC constant region ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... were transfected at cell densities between 8.0×105 to 1.0×106 cells/ml with equal masses of plasmids containing the heavy chain and light chain genes using FreeStyleTM MAX Reagent (ThermoFisher Scientific 16447100); both plasmids were diluted in OptiPROTM SFM (ThermoFisher Scientific 12309050) ...
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bioRxiv - Microbiology 2021Quote: ... The antibody production was conducted by co-transfection of the light chain and heavy chain sequences into Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific, A14635). The culture supernatant was collected at 7 days post-transfection ...
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bioRxiv - Immunology 2020Quote: ... Four µg of the heavy chain and 4 µg of the corresponding light chain were mixed together in 375 µl Opti-MEM (Gibco, ThermoFisher Scientific, UK) before addition of polyethylenimine (PEI MAX (Polysciences ...
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bioRxiv - Cell Biology 2022Quote: Plasmids pcDNA3.4 comprising heavy chains and light chains of antibodies were transiently expressed in ExpiCHO-S cells using the ExpiCHO™ expression system (Thermo Fisher). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were co-transfected by polyethylenimine-transfection to Expi293F™ cell (ThermoFisher, A14527). Cells were cultured at 36.5°C ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Synthetic Biology 2020Quote: ... by diluting heavy and light chain construct DNA in OptiMem serum-free media (Gibco) before the addition of and incubation with FectoPro (Polyplus Transfection ...