-
No products found
because this supplier's products are not listed.
Brian R. Graziano, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio) per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Priyanka Dutta Gupta, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the cells were harvested and cell lysates processed using recommended buffers (cGAMP detection kit, Cayman Chemical) and then used for incubation with anti-cGAMP antibody and detection conjugate for 2 hours or overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
-
No products found
because this supplier's products are not listed.
W.J. Venema, et al.,
bioRxiv - Immunology 2021
Quote:
... S-antigen (α-mGFP, TA180076, Origene, to detect the fusion protein S-antigen-GFP ...
-
No products found
because this supplier's products are not listed.
Kira Allmeroth, et al.,
bioRxiv - Physiology 2022
Quote:
... Mice expressing the cre recombinase under the control of the human cytomegalovirus minimal promoter (CMV-cre+/-) were purchased from Charles River Laboratories (Sulzfeld ...
-
No products found
because this supplier's products are not listed.
Sierra Fox, et al.,
bioRxiv - Genetics 2019
Quote:
... Cell lysate was resuspended in transposition reaction mix (Illumina) and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Claudio Schmidli, et al.,
bioRxiv - Biophysics 2019
Quote:
... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
-
No products found
because this supplier's products are not listed.
Jessica R. Eisenstatt, et al.,
bioRxiv - Genetics 2020
Quote:
... Cell lysates were generated by homogenizing cells with a FastPrep-24 5G homogenizer (MP Biomedicals) and a fraction of the lysate was aliquoted for input ...
-
No products found
because this supplier's products are not listed.
Nathan A. Dahl, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
-
No products found
because this supplier's products are not listed.
Paul J. Hoover, et al.,
bioRxiv - Immunology 2023
Quote:
... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
-
No products found
because this supplier's products are not listed.
Kelly Urbanek, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Cells were imaged for reovirus antigen and DAPI using a Lionheart FX automated imager (BioTek) equipped with a 20X air objective ...
-
No products found
because this supplier's products are not listed.
Muriel E. Babey, et al.,
bioRxiv - Neuroscience 2024
Quote:
... with 10% human platelet-derived lysate (Stem Cell Technologies, Cat#06960) and 1% Penicillin-Streptomycin solution (Pen-Strep ...
-
No products found
because this supplier's products are not listed.
Jeffrey R. Johnson, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel) and proteins were transferred to nitrocellulose membranes ...
-
No products found
because this supplier's products are not listed.
Ling Wei, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Cell lysates were clarified in filter plates (Pall Cat# 8075) at 3500 rpm for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Koen M.A. Dreijerink, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Total cell lysates were analyzed through immunoblotting using anti-menin (A300-105A, Bethyl) and anti-alpha tubulin (CP06 Calbiochem ...
-
No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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No products found
Nithya Gajendran, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Lentivirus particles expressing human α-syn under cytomegalovirus (CMV) promoter (Applied Biological Materials, Inc, Canada) was used to re-express α-syn in SK-MEL-28 KO cells as described previously30 ...
-
No products found
because this supplier's products are not listed.
Manutsanun Inthawong, et al.,
bioRxiv - Immunology 2022
Quote:
... A T cell antigen control pool (CEF, Mabtech AB, 3616-1) at a final concentration of 1 μg/ml was used as a source of control antigens ...
-
No products found
because this supplier's products are not listed.
Christopher M. Schafer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Incubation of cell lysates with polyubiquitin-affinity beads (Calbiochem: #662200-1KIT) was carried out according to the manufacturer’s protocol followed by immunoblotting with an anti-ERG Ab (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
Nuno Apóstolo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1:50 donkey anti-mouse IgG antigen-binding fragments (Jackson ImmunoResearch). Sections were then washed in PBS-0.5%Triton X-100 at RT and incubated at 4°C overnight with primary antibodies in PBS containing 5% NHS ...
-
No products found
because this supplier's products are not listed.
Brandon Novy, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Fluorescence of both lysate and intact cell assays measured with a Spectra Max Gemini XPS (Molecular Devices), excitation 561 nm and emission 610 nm ...
-
No products found
because this supplier's products are not listed.
Eul Sik Jung, et al.,
bioRxiv - Neuroscience 2020
Quote:
Cell lysates were prepared using RIPA buffer (Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
Sylvia Varland, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar) and denatured at 95°C for 5 min ...
-
Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in...
Cat# LK003265,
5 ea, $50.00
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Adam K. Wade-Vallance, et al.,
bioRxiv - Immunology 2022
Quote:
... Biosearch Technologies]) or control antigen (ovalbumin; OVA [Worthington Biochemicals]) in 20µl PBS by subcutaneous injection ...
-
No products found
because this supplier's products are not listed.
Steven V. John, et al.,
bioRxiv - Cell Biology 2024
Quote:
... gondii surface antigen 1 (SAG1, rabbit, 1:300; Genetex GTX38936) overnight at 4°C and then thoroughly washed with DPBS ...
-
No products found
because this supplier's products are not listed.
Tingting Geng, et al.,
bioRxiv - Immunology 2021
Quote:
... Human embryonic kidney 293 cells transformed with T antigen of SV40 (HEK293T, # CRL-3216) and Vero cells (monkey kidney epithelial cells, # CCL-81) were purchased from American Type Culture Collection (ATCC) (Manassas ...
-
No products found
because this supplier's products are not listed.
Peter S J Bailey, et al.,
bioRxiv - Cell Biology 2020
Quote:
OGDHc activity was measured in whole cell lysates using a Biovision ketoglutarate dehydrogenase activity assay (Biovision K678), according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Maria C. Sterrett, et al.,
bioRxiv - Genetics 2022
Quote:
... Lysates were placed in a Mini Bead Beater 16 Cell Disrupter (Biospec) for 6 × 1 min at 25°C with ice submersion intervals of 1 minute between rounds ...
-
No products found
because this supplier's products are not listed.
Nicholas M. Chesarino, Michael Emerman,
bioRxiv - Evolutionary Biology 2021
Quote:
... Cell lysate was measured for luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech). Raw luciferase values were normalized to 2,000 mU RT activity for each virus ...
-
No products found
because this supplier's products are not listed.
Dongying Chen, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... To harvest protein lysate from IBIDI µSlide ...
-
No products found
because this supplier's products are not listed.
Kelly Ceyzériat, et al.,
bioRxiv - Pathology 2022
Quote:
... under the control of a cytomegalovirus promoter (ViraPower Adenovirus Expression System, Invitrogen). The AAV-Tau was verified by sequencing and the production of Tau by western blot ...
-
No products found
because this supplier's products are not listed.
Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Antigen retrieval was performed using Tissue-Tek antigen retrieval solution (IHC World) for 1 hour in a steamer ...
-
No products found
because this supplier's products are not listed.
Scott Johnson, et al.,
bioRxiv - Physiology 2023
Quote:
... Recombinant adenovirus encoding N-terminally FLAG-tagged mouse PNPLA3 under the control of a cytomegalovirus (CMV) promoter (Ad-FLAG-PNPLA3) was custom generated by Vector Biolabs. A CMV-null adenovirus (Ad-Null)] was also obtained for use in control experiments ...
-
No products found
because this supplier's products are not listed.
Jacob T. Martin, et al.,
bioRxiv - Immunology 2020
Quote:
... The vector with antigen-bearing component was transfected into FreeStyle 293F cells using polyethylenimine (Polysciences, Inc) as described previously 7 ...
-
No products found
because this supplier's products are not listed.
Vikas A. Tillu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cleared cell lysates were prepared using a continuous flow cell disruptor (Constant Systems Limited, UK) at pressure range 25 – 30 kPsi with subsequent addition of 0.5 % w/v Triton X-100 (Cavin1 purification ...
-
No products found
because this supplier's products are not listed.
Dobromir Szadkowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... and cell lysate filtered through 0.45 μm Polysulfone filter (Filtropur S 0.45, Sarstedt). The cleared cell lysate was loaded onto a 5mL HiTrap Chelating HP column (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Andrea R. Shiakolas, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
-
No products found
because this supplier's products are not listed.
Lorène Gonnin, et al.,
bioRxiv - Microbiology 2023
Quote:
... the luciferase activities were determined for each cell lysate with an Infinite 200 Pro (Tecan, Männedorf, Switzerland) and normalised based on β-galactosidase (β-Gal ...
-
No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cell lysates were prepared using the sample preparation kit (Protein Simple) for an automated capillary western blot system ...
-
No products found
because this supplier's products are not listed.
Tiphany Chrun, et al.,
bioRxiv - Immunology 2021
Quote:
... Abundance of viral antigen was assessed using the influenza NP staining (HB-65 mAb, Bio X Cell, Lebanon, USA) and was scored as described previously (25) ...
-
No products found
because this supplier's products are not listed.
Júlio Souza dos-Santos, et al.,
bioRxiv - Immunology 2022
Quote:
Antigen-specific IgG (Cat. No. 1015-05, Southern Biotech, AL, USA), IgA (Cat ...
-
No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Antigen for immunization was prepared by mixing NP-OVA (Biosearch Technologies), 10% KAL(SO4)2 dissolved in PBS at a ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Maibam R. Singh, Jozsef Vigh, Gregory C. Amberg,
bioRxiv - Neuroscience 2021
Quote:
... we used transgenic mice expressing ChR2 fused to YFP under the control of mouse thymus cell antigen 1 (Thy 1) promoter (stock #007612, Jackson laboratory), which specifically express ChR2 in SNr GABA neurons ...
-
No products found
because this supplier's products are not listed.
Marina Utkina, et al.,
bioRxiv - Genomics 2024
Quote:
... Visualization of the antigen-antibody complexes was performed using an Olympus FV3000 Scanning Confocal Microscope (Olympus corporation, Tokyo, Japan).
-
Cat# HY-P0277-500 μg,
500 μg, USD $216.0
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Heather Ann Bruce, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cell culture expressing antigen- B was supplemented with 5 mM Kifunensine (MedChemExpress) to inhibit mannosidase I activity ...
-
No products found
because this supplier's products are not listed.
Wei Deng, et al.,
bioRxiv - Microbiology 2020
Quote:
... an antigen retrieval kit (AR0022, Boster) was applied to the sections at 37°C for 1 min ...
-
No products found
because this supplier's products are not listed.
Jesse M. Hall, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antigen specific antibody titers to PT (List Biological Laboratories #180) were measured by coating ELISA plates with 50 μl of antigen per well ...
-
No products found
because this supplier's products are not listed.
Asmaa H. Mahmoud, et al.,
bioRxiv - Immunology 2024
Quote:
All bacteria-cell lysates were treated with propidium monoazide (PMA, Biotium, Fremont, CA, USA), a photoreactive dye that effectively eliminates PCR amplification of DNA from dead cells ...
-
No products found
because this supplier's products are not listed.
Bahareh Haddad Derafshi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... we dissociated stem cells into single cells with Accutase (Innovative Cell Technologies) and seeded at ~ 40K cells into one 24 well plate pre-coated with Matrigel (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Alícia C. Piffer, et al.,
bioRxiv - Immunology 2021
Quote:
... HUVECs cells (2.5×105 cells/mL - PromoCell), resuspended in free endothelial medium ...