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Citations for Active Motif :
1 - 50 of 128 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
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bioRxiv - Cancer Biology 2024Quote: RAS GTPase activity in cell lysate of the WT CaSki or ΔPr-A9 cells was determined by RAS GTPase Chemi ELISA Kit (Active Motif, Carlsbad, CA, # 52097) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were sonicated (EpiShear Probe Sonicatore, Active Motif) for 10 cycles with one second intervals and a 20% amplitude using ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Lysates were sonicated (EpiS-hear Probe Sonicatore, Active Motif) for 10 cycles with one second intervals and a 20% amplitude ...
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bioRxiv - Genomics 2019Quote: ... Precleared lysates were incubated with anti-γH2AX (Active Motif, #39117) at 4 °C for overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... lysates were prepared using the Histone Extraction Kit (Active Motif) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear lysates were prepared using the Nuclear Extracts Kit (Active Motif, # 40010). 20-50 ug Proteins were separated by SDS-PAGE and transferred into nitrocellulose membrane using the iBlot Transfer Stacks and the iBlot2 transfer system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear and cytoplasmic protein lysates were prepared using the Nuclear Extract Kit (Active Motif) or Nuclear Complex Co-IP Kit (Active Motif) ...
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bioRxiv - Cell Biology 2021Quote: ... precleared lysates were adjusted to 1X high stringency IP buffer (Active Motif, cat# 37510) with protease/phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2024Quote: 4T1 nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif).
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bioRxiv - Synthetic Biology 2022Quote: The custom rabbit polyclonal antiserum against PARP-1 was generated in-house by using purified recombinant amino-terminal half of PARP-1 as an antigen (now available Active Motif; cat. no. 39559). The custom recombinant antibody-like anti-poly-ADP-ribose binding reagent (anti-PAR ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were transferred to a new tube with 50 μl Protein-G Dynabeads (Active Motif) and further incubated for 1 hr before following the manufacturer’s recommendations for washing of the beads and library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear lysates of each tissue specimen were incubated with 5 μg of H3K27ac antibody (Active Motif, 39133) pre-bound to 50 µL magnetic protein A dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... TransAM p65 NF-κB activation assay was performed using nuclear lysate as per standard protocol (Active Motif). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated and the DNA sheared to an average length of 300-500bp using the EpiShear™ Probe Sonicator (Active Motif, cat # 53051) with an EpiS-hear™ Cooled Sonication Platform (Active Motif ...
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bioRxiv - Genomics 2020Quote: ChIP for small cell numbers was performed with a Low Cell ChIP-Seq Kit (Active Motif) according to the manufacturer’s manual (version A3 ...
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bioRxiv - Microbiology 2019Quote: ... Cell fixation was performed using the fixation reagents and steps from the Low Cell Chip Kit (Active Motif; Cat#53084) as described in the user manual ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Biophysics 2020Quote: A HeLa cell sample (Active Motif/Leica Microsystems) was used for evaluation of the alignment of 2D STED imaging of a realistic biological sample ...
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bioRxiv - Cancer Biology 2023Quote: 2 × 107 cells for H3K27ac (Active Motif, Cat # 39133) and 3 × 107 cells for IRF4 (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2023Quote: ... the Low Cell ChIP-Seq Kit from Active Motif was used (53084) ...
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bioRxiv - Genetics 2023Quote: ... and ∼1 × 107 cells for H3K27ac (Active Motif, 39133). Cells were crosslinked with 1% formaldehyde for 10 min at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then incubated in MAXBlock Blocking medium (Active Motif) supplemented with final concentrations of 0.1% Triton X-100 and 100 mM glycine for 20 minutes at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in 100ul chromatin sheering buffer (Active Motif) and placed on 96 well plate sealed with PCR film (MiniAMP Optical Adhesive Film ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated with primary antibody (H3K4me3, Active motif #39160 ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA from in vitro methylated Jurkat cells were (Active Motif) served as positive control and genomic DNA from H1993 cells served as negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... we followed the ChIP Cell Fixation protocol provided by Active Motif. Cells were fixed by adding 1/10th volume of freshly prepared solution of 11% Formaldehyde (SIGMA #F-8775) ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif, Anti H3 #9715 Cell Signaling), detection protocols have been summarized in Supplemental Table 4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were incubated with primary antibodies in MAXStain Staining medium (Active Motif) at 1:500 overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and transformed into Rapid Trans™ chemically competent TAM1 cells (Active Motif).
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Genomics 2023Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-dCENP-A for Kc167 cells (AB_2793749, 1:100, Active motif), rabbit anti-dCENP-A for ovaries (AB_2793320 ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used: EZH1 (Cell Signalling, 42088) EZH2 (Active Motif, 39933), H3K27m3 (Cell Signalling ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunoprecipitation according to the Low Cell ChIPseq kit (Active Motif, 53084) with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 1:200 dilution of PARP2 antibody (Active Motif, 39743) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl (50 ng) of Drosophila chromatin from S2 cells (Active Motif, #53083) was added as spike-in control ...
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bioRxiv - Microbiology 2021Quote: ... RASGTP levels in cells were quantified using RASGTP ELISA kit (Active Motif, 52097) per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with rabbit polyclonal antibodies against H3K9me3 (1:1000; Active Motif) in the 1XPBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 mM dithiothreitol (DTT)) and sonicated for cell lysis (Q120 Sonicator, Active Motif). After centrifugation for 1 h at 4 °C and 45,850 g ...
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bioRxiv - Genomics 2023Quote: ... 0.5 million cells were processed using the CUT&Tag-IT kit (Active Motif) as per manufacturer’s instructions and the resulting libraries were paired-end sequenced on a NextSeq500 platform (Illumina ...