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No products found
because this supplier's products are not listed.
Manal Ibrahim Kosta, et al.,
bioRxiv - Pathology 2020
Quote:
... VWF antigen was measured with a commercially available enzyme-linked immunosorbent assay kit from Diagnostica Stago.
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No products found
because this supplier's products are not listed.
Meredith J. Sigman, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Clarified lysates were combined with 2 μL of AGO4 antibody (Agrisera) or 2uL of rabbit IgG as a mock IP (Cell Signaling Technology) ...
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No products found
because this supplier's products are not listed.
Nikol Dibus, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lysates were incubated with the PD-1 antibody (1 µg; Exbio #11-176) and mixed with Dynabeads® Protein G ...
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No products found
because this supplier's products are not listed.
Sloan A. Lewis, et al.,
bioRxiv - Immunology 2021
Quote:
Circulating endotoxin was measured from plasma using a Limulus amebocyte lysate (LAL) assay (Hycult Biotech) following the manufacturers protocol.
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No products found
because this supplier's products are not listed.
Rosalie Lipsh-Sokolik, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
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No products found
because this supplier's products are not listed.
Lining Zheng, Sarith R. Bandara, Cecilia Leal,
bioRxiv - Bioengineering 2022
Quote:
... 4T1 cells (ATCC) and HeLa-Luc cells (Signosis) were cultured in full cell media consisting of Dulbecco’s modified Eagle’s medium (Corning) ...
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No products found
because this supplier's products are not listed.
Nirdosh Dadwal, et al.,
bioRxiv - Biochemistry 2021
Quote:
For cell surface expression on Jurkat T cells 0.2 × 106 cells were incubated with anti-CD18 (clone MEM-48, antibodies-online.de), anti-CD184 (CXCR4 ...
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This kit is faster, stable, and more sensitive than the traditional soft-agar assay.
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Yunjeong Kim, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero E6 cells expressing human TMPRSS2 (Vero-TMPRSS2) cells were obtained from Creative Biogene (Shirley, NY)[88] ...
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No products found
because this supplier's products are not listed.
Beerend H.J. Winkelman, et al.,
bioRxiv - Neuroscience 2019
Quote:
... amplified (X3+Cell; FHC, Bowdoinham, ME), digitized at 15 kHz (Power1401 ...
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No products found
because this supplier's products are not listed.
Christine Salaun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Sf9 cells (Oxford Expression Technologies #600100) were plated in 6-well plates and co-transfected with 1.2 μl of baculoFectin II ...
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No products found
because this supplier's products are not listed.
Didem Ilter, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... HEK293T cells were obtained from GenHunter. All cell line were maintained at 37°C and 5% CO2 in the presence of 100 unit/ml penicillin and 100 μg/ml streptomycin (Gibco ...
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No products found
because this supplier's products are not listed.
Jen M. Hope, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
PC12 cells (NeuroScreen-1 subclone, Cellomics, discontinued) were maintained in complete medium (F12K supplemented with 15% horse serum and 2.5% FBS ...
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No products found
because this supplier's products are not listed.
Rosalia Paterno, et al.,
bioRxiv - Neuroscience 2024
Quote:
Concentrated GFP+ MGE cells (∼600 cells per nl) were injected using 30° beveled glass micropipettes (80 μm diameter tip, Witerol 5 μl, Drummond Scientific) prefilled with mineral oil into cortex or hippocampus of recipient pup (P2-4) ...
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No products found
because this supplier's products are not listed.
Lucia Leitner, et al.,
bioRxiv - Developmental Biology 2024
Quote:
Total RNA was extracted from single cell suspensions of 500,000 CSCs or CB-derived cells using the FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit (Favorgen, FABRK001-2), eluted with 40μl RNase-free ddH2O and the RNA concentration was measured with a Nanodrop 2000c spectrophotometer (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Shivani C. Kharod, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cells were treated with primary antibodies: anti-GFP (Aves lab) or anti-FMRP (Abcam ...
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No products found
because this supplier's products are not listed.
Keng Hou Mak, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and were maintained in mesothelial cell growth medium (MSO-1, Zen-Bio, Inc.). Human cervical adenocarcinoma cell line HeLa from American Type Culture Collection was maintained in DMEM medium supplemented with 10% fetal bovine serum ...
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No products found
because this supplier's products are not listed.
Bruno Raposo, et al.,
bioRxiv - Immunology 2022
Quote:
... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
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No products found
because this supplier's products are not listed.
Maria Tokuyama, et al.,
bioRxiv - Immunology 2019
Quote:
... Ro-SSA antigen was purchased (Arotec Diagnostics) and HA and tetanus proteins were obtained from ImmuneTechnology Corp ...
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No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIV antigen GP120 was purchased from Immune Technology Corporation (IT-001-022p) ...
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No products found
because this supplier's products are not listed.
Julia Bitencourt, et al.,
bioRxiv - Immunology 2021
Quote:
... The antigens used were purified protein derivative (PPD) from M.tb (Statens Serum Institut (SSI), Denmark ...
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No products found
because this supplier's products are not listed.
Rueyhung R. Weng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5% human platelet lysate (PLS1, Compass Biomedical), 70 ng/mL IL-15 (AF-200-15 ...
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No products found
because this supplier's products are not listed.
Samarpan Maiti, et al.,
bioRxiv - Cell Biology 2023
Quote:
Immunoblot analyses: Lysates of cells (20–100 μg) were subjected to SDS-PAGE and transferred onto a nitrocellulose membrane (GVS Life Science) with a wet blot transfer system (VWR #BTV100) ...
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No products found
because this supplier's products are not listed.
Paul A. Rowley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
Human brain and testes tissue total protein lysates were purchased from ProSci Incorporated (catalogue numbers 1303 and 1313 ...
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No products found
because this supplier's products are not listed.
Milène Vandal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The lysate was incubated with 100 U/mL salt-active nuclease (Arcticzymes) at 37°C for 1 h and then centrifuged at 2000 xg for 15 min ...
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No products found
because this supplier's products are not listed.
Aleksi Isomursu, et al.,
bioRxiv - Biophysics 2020
Quote:
Cells that were collected for protein lysates were cultured on commercial hydrogel-coated 6-well plates (Matrigen, SW6-EC-0.5/SW6-EC-8/SW6-EC-50). These gels were similarly coated with 10 μg/ml of fibronectin before use.
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No products found
because this supplier's products are not listed.
Eva Mittler, et al.,
bioRxiv - Microbiology 2020
Quote:
... cleared U2OS-Cas9 lysates were pre-incubated with the protease inhibitor E-64 (20 μM; Peptides International) for 20 min at room temperature (when indicated) ...
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No products found
because this supplier's products are not listed.
William Tyler Frantz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells from BMP-KO samples as well as non-melanocyte lineage keratinocytes were then removed ...
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No products found
because this supplier's products are not listed.
Sanam Shafaatalab, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The cells were counted using the CellDrop automated cell counter (Froggabio, ON). The lysis buffer used contained 100 mM HEPES (pH= 8-8.5 ...
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No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... All secreted proteins were produced by using the transient Icosagen Cell Factory system with CHOEBNALT-85 cells and the QMCF Technology (Icosagen Cell Factory OÜ, Tartu, Estonia). Cells were maintained in a 50:50 mixture of 293 SFM II (Gibco ...
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No products found
because this supplier's products are not listed.
Zixuan Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell counting kit-8 (M4839, AbMole).
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No products found
because this supplier's products are not listed.
K.G. Daniels, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... T cells were cryopreserved in RPMI1640 (UCSF cell culture core) with 20% human AB serum (Valley Biomedical, #HP1022HI) and 10% DMSO ...
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No products found
because this supplier's products are not listed.
M.A. Andres, et al.,
bioRxiv - Neuroscience 2022
Quote:
Human neural progenitor cells (hNPCs) (Neuromics, MN) were plated on Matrigel (Corning ...
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No products found
because this supplier's products are not listed.
Oihana Iriondo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... cells were moved to hypoxic incubators (Nuaire NU-5831 for 4% O2 ...
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No products found
because this supplier's products are not listed.
Sumeet Nayak, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... spotted (2.5μl of 105 cells/ml PBS cell suspension) and lysed on positively charged microscope slides (Globe Scientific, #1358W) by 7.5μl spreading buffer (0.5%SDS ...
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No products found
because this supplier's products are not listed.
Shisu Zhao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cell counting kit 8 (CCK8) experiment for cell viability assay were performed using the CCK8 Kit (TargetMol, MA, USA). After a treatment ...
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No products found
because this supplier's products are not listed.
Barbara Oldrini, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 4-5 weeks old immunodeficient nu/nu mice were then intracranially injected with the sorted cells (5 x105 cells) using a stereotactic apparatus (Stoelting). After intracranial injection ...
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No products found
because this supplier's products are not listed.
Ryan S. Dhindsa, et al.,
bioRxiv - Genomics 2020
Quote:
... strained through 40 um cell strainers (MTC Bio) to remove cell clumps and collected for single-cell RNA-sequencing (scRNA-seq ...
-
No products found
because this supplier's products are not listed.
Juline Poirson, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Cells were treated with 1 µM MLN4924 (Chemietek) for 24 hours ...
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No products found
because this supplier's products are not listed.
Midori Ohta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... coding sequences optimized for human cell expression were synthesized (GENEWIZ). Sequences encoding Myc-tagged GIP-2 ...
-
No products found
because this supplier's products are not listed.
Ramireddy Bommireddy, et al.,
bioRxiv - Immunology 2022
Quote:
... and then 0.5% chicken red blood cells (Lampire Biological Laboratories) were added to determine HAI titers ...
-
No products found
because this supplier's products are not listed.
Makaía M. Papasergi-Scott, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were treated with 5 μM CGP55845 (Hello Bio, Inc.). At 48 hours post-infection cells were harvested by centrifugation ...
-
No products found
because this supplier's products are not listed.
Frank W. Soveg, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were seeded on #1.5 12-mm coverslips (Bioscience Tools) coated with 10 μg/mL poly-L-ornithine hydrobromide (Sigma ...
-
No products found
because this supplier's products are not listed.
Stephen K. Dolan, et al.,
bioRxiv - Microbiology 2023
Quote:
... the cells were grown in Turbo Broth medium (Molecular Dimensions) (1 L ...
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No products found
because this supplier's products are not listed.
Oded Danziger, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cell lines were routinely tested (Boca Scientific #50-168-5641) negative for Mycoplasma contamination ...
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No products found
because this supplier's products are not listed.
Tamara A. Potapova, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Transfected cells were selected with 1 mg/ml G418 (A.G. Scientific).
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No products found
because this supplier's products are not listed.
Tugsan Tezil, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Histones were extracted from HEK293T cells by EpiQuik Total Histone Extraction Kit (Epigentek), following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Elisa Matas-Rico, et al.,
bioRxiv - Cancer Biology 2021
Quote:
T cell migration was measured using 48-well chemotaxis chambers (Neuro Probe, Inc.) equipped with 5 µm-pore polycarbonate membranes (8 µm-pore for melanoma cells) ...
-
No products found
because this supplier's products are not listed.
Harry C. Tjondro, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Degranulated MPO (Dg-MPO) and cell death were monitored longitudinally in supernatants using ELISA (ICL LAB) and lactate dehydrogenase (LDH ...
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No products found
because this supplier's products are not listed.
Yusuke Kato, et al.,
bioRxiv - Plant Biology 2023
Quote:
... sulfur-starved cells were labeled with 5 μ Ci mL-1 [35S]Na2SO4 (American Radiolabeled Chemicals) in the light at 50 µmol photons m-2s-1 in the presence of 10 µg mL-1 cycloheximide ...
-
No products found
because this supplier's products are not listed.
JN Rashida Gnanaprakasam, et al.,
bioRxiv - Immunology 2022
Quote:
... and glutamate in blank medium (without cells) and spent medium were measured by the bioanalyzer (YSI 2900). The metabolite consumption and production were determined by calculating the difference between blank and spent medium ...