-
No products found
because this supplier's products are not listed.
Lilian M. Fennell, et al.,
bioRxiv - Cell Biology 2019
Quote:
... pOPINK-vOTU (CCHFV OTU, aa1-183) (Addgene plasmid #61589) (Akutsu et al. ...
-
No products found
because this supplier's products are not listed.
Diem Hong Tran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
-
No products found
because this supplier's products are not listed.
Raphael R. Eguchi, et al.,
bioRxiv - Biochemistry 2022
Quote:
... from various snake species were selected from NCBI databases and cloned into a mammalian expression vector containing a C-terminal Avi™ tag followed by a WELQut™ cleavage tag and a rabbit Fc tag by Genscript. Plasmid DNA was prepped and co-transfected with a plasmid expressing the BirA enzyme for in vivo biotinylation into Expi293 cells using FectoPRO® (Polyplus Transfection) ...
-
No products found
because this supplier's products are not listed.
Edward Courchaine, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Congo Red (0.005%, Sigma), or Proteostat (3 μM ...
-
No products found
because this supplier's products are not listed.
Huiru Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
-
No products found
because this supplier's products are not listed.
Eva Fisher, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera) (ab272105, Abcam) was used as positive control (loaded 2.4 ug/lane) ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
-
No products found
because this supplier's products are not listed.
Maria C. Virgilio, et al.,
bioRxiv - Microbiology 2024
Quote:
... sheep (rabbit anti-sheep IgG, Dako), and human (goat anti-human IgG ...
-
No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... expressing Moloney murine leukemia virus gag and pol genes was co-transfected with pLNCX2 vector with the FLAG-APEX2-GARG1060 insert and a plasmid coding for the vesicular stomatitis virus envelope glycoprotein (Takara Bio) using Mirus 2020 DNA transfection reagent (Mirus) ...
-
No products found
because this supplier's products are not listed.
Hangu Nam, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Fc Tag (ACROBiosystems). To obtain comparable live cell counts between conditions ...
-
No products found
because this supplier's products are not listed.
David Smart, et al.,
bioRxiv - Microbiology 2019
Quote:
... and vesicular stomatitis virus (VSV) tag (Biolegend UK Ltd, London, UK) followed by the appropriate secondary peroxidase-conjugated antibody (Sigma ...
-
No products found
because this supplier's products are not listed.
Zeyu Sun, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
-
No products found
because this supplier's products are not listed.
Pan Liu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed with 100 ng Tie2‐Fc (with His tag, R&D systems) in TBST buffer at 4°C for 4 hours ...
-
No products found
because this supplier's products are not listed.
Yuriy Pomeshchik, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Congo Red staining was performed using Congo Red Staining Kit (Merck) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Matthew H. Raymonda, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Kimberley M. Mellor, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... with AAV9-cTnT-Gabarapl1 or AAV9-cTnT-Null virus (10,000 gc/cell; Vector Biolabs) for 24 hours ...
-
No products found
because this supplier's products are not listed.
Olusegun O. Onabajo, et al.,
bioRxiv - Genetics 2020
Quote:
... ACE2 with C-terminal GFP-tag (RG208442) and Myc-DDK tag (RC208442) were purchased from Origene. Empty vectors pMax-GFP (Lonza ...
-
No products found
because this supplier's products are not listed.
Brittany F. Karas, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Ana C. Almeida, et al.,
bioRxiv - Cell Biology 2021
Quote:
... α-sheep-HRP (Jackson ImmunoResearch). Detection was performed with Clarity Western ECL Substrate (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Chris G.C. Jacobs, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... Sheep anti-dioxigenin-AP antibody (Roche) was used 1:5000 in blocking solution [40].
-
No products found
because this supplier's products are not listed.
Jianying Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus mixtures were subsequently mixed at a ratio of 1:1 with PBS-washed sheep blood cells (Hemostat laboratories, Galveston, TX, USA). Aliquots of blood meals were collected to verify the initial virus proportions in the inoculum ...
-
No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... and yellow fever E antibody 3576 (Santa Cruz, sc-58083, for YF17D). The primary antibodies were detected with an Alexa-488 conjugate secondary antibody (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Kenji Ichiyama, et al.,
bioRxiv - Immunology 2023
Quote:
... or anti-c-Myc-Tag (Cell Signaling Technology). The membrane was then probed with appropriate secondary antibodies conjugated to HRP and visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Fang Tian, et al.,
bioRxiv - Biophysics 2021
Quote:
... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
-
No products found
because this supplier's products are not listed.
Peng He, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ...
-
No products found
because this supplier's products are not listed.
Sunsook Hwang, et al.,
bioRxiv - Immunology 2023
Quote:
... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
-
No products found
because this supplier's products are not listed.
Shijian Zhang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
-
No products found
because this supplier's products are not listed.
Yan Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
-
No products found
because this supplier's products are not listed.
Amanda M. Travis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and C-terminal MYC tag was placed in front of human NPHP3 (GeneCopoeia GC-H2370). All mutations to replace the lipidated cysteine with alanine were made using PCR mutagenesis (Weiner et al. ...
-
No products found
because this supplier's products are not listed.
Deniz Durmusoglu, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... A primary antibody (c-myc tag Polyclonal Antibody, HRP, Bethyl Laboratories) solution was prepared at 1:10000 dilution in 1xTBS with 0.5% Tween-20 buffer ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... anti-mouse protein C polyclonal sheep IgG conjugated with horse-radish peroxidase (GeneTex, Irvine, CA) was incubated for 1 h ...
-
No products found
because this supplier's products are not listed.
Anja M. Touma, et al.,
bioRxiv - Biophysics 2022
Quote:
... construct with a C-terminal eGFP tag and N-terminal FLAG tag was generated and subcloned into a shuttle vector provided by Vector Laboratories, Burlingame ...
-
No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
-
No products found
because this supplier's products are not listed.
Min Jiang, Changyin Fang, Yongping Ma,
bioRxiv - Immunology 2023
Quote:
... Mouse anti-c-MYC-tag and anti-Flag-tag mAb were purchased from Proteintech(cat# 6003-2-lg/66008-3-lg ...
-
No products found
because this supplier's products are not listed.
Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... sheep anti-TGM2 (Novus Biologicals, AF5418), and rat anti-GFAP (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
David W. Hawman, et al.,
bioRxiv - Immunology 2020
Quote:
An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
-
No products found
because this supplier's products are not listed.
Poorwa Kamal Badola, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Supernatant were used for analysis through GC-MS (PerkinElmer Mass Spectrometer Clarus SQ 8 C). The calibration curve was made using Synthetic IAA (Sigma).
-
No products found
because this supplier's products are not listed.
Francisco J. Salguero, et al.,
bioRxiv - Microbiology 2020
Quote:
... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ...
-
No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Mohamed H. Elsafi Mabrouk, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 20% FCS (Lonza), 2 mM L-Glutamine ...
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2023
Quote:
... Env trimers with C-terminal avi-tags (Avidity) were biotinylated and conjugated to streptavidin labeled with different fluorochormes ...
-
No products found
because this supplier's products are not listed.
Berenike Kircher, et al.,
bioRxiv - Microbiology 2022
Quote:
... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
-
No products found
because this supplier's products are not listed.
Colten Lankford, Jon Houtman, Sheila Baker,
bioRxiv - Biochemistry 2021
Quote:
... Lysates were quantified by BCA assay and equal total protein concentrations were analyzed by western blotting against the SNAP-tag with membranes imaged for SNAP-HCN1 conjugated SNAP-Cell TMR-star (600 nm channel on LI-COR Odyssey FC) prior to blocking.
-
No products found
because this supplier's products are not listed.
Kamyab Javanmardi, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The plasmid expressing the VSV-G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The lentiviral plasmid used to generate the HEK-293T stable cell lines expressing the human ACE2 gene (GenBank ID NM_021804 ...
-
No products found
because this supplier's products are not listed.
Carolin Gerke, et al.,
bioRxiv - Microbiology 2023
Quote:
... FCS (PAN Biotech), 1 % (v/v ...
-
No products found
because this supplier's products are not listed.
Fangzhu Zhao, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 25 µL/well of diluted samples were then incubated with 25 µL/well of pseudotyped virus for 1 h at 37 °C in 96-well half- area plates (Corning, 3688). After that ...
-
No products found
because this supplier's products are not listed.
Madlen Merten, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
-
No products found
because this supplier's products are not listed.
Kuan-Ting Huang, et al.,
bioRxiv - Biochemistry 2022
Quote:
Fluorescence stereomicroscope (e.g., Leica M165 FC)